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Weissella confuse strain with antibacterial and antioxidant activity and application thereof

Antioxidant activity, technology of Weissella, applied in the field of microbiology

Active Publication Date: 2020-07-03
CHINA PHARM UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

As a new lactic acid bacterium, Weissella is widely used in fermented food, but there are few reports on its use as a probiotic. More attention is needed on the basic research on the probiotic activity of this type of bacterium

Method used

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  • Weissella confuse strain with antibacterial and antioxidant activity and application thereof
  • Weissella confuse strain with antibacterial and antioxidant activity and application thereof
  • Weissella confuse strain with antibacterial and antioxidant activity and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Pathogen antagonism test

[0024] The antagonistic activity of fusion Weissella YM5S2 against pathogenic bacteria was detected by punching method. The fusion Weissella YM5S2 was inoculated in MRS liquid medium, and incubated at 37° C. for 24 hours. The bacteria-containing liquid medium was centrifuged at 12000 r / min for 10 min, and the supernatant was filtered through a 0.22 μm filter membrane to obtain a cell-free supernatant. Escherichia coli, Staphylococcus aureus, and Salmonella typhimurium were inoculated in nutrient broth medium respectively, and cultured at 37°C for 18 hours as indicator bacteria for later use. Mix 100 μL of indicator bacteria solution into 100 mL of sterile nutrient broth solid medium, and pour it into a petri dish. Take 60 μL of the above-mentioned cell-free supernatant and add it to the well (5mm) of the plate containing the indicator bacteria. After culturing overnight at 37°C, measure and record the diameter of the inhibition zone.

[002...

Embodiment 2

[0027] Antioxidant activity detection

[0028] The fusion Weissella YM5S2 was inoculated in MRS liquid medium, and incubated at 37° C. for 24 hours. The bacteria-containing liquid medium was centrifuged at 12,000 r / min for 10 min, and the supernatant was filtered through a 0.22 μm filter membrane to obtain a cell-free supernatant.

[0029] a. DPPH free radical scavenging activity

[0030] 0.4 mL of the above cell-free supernatant was mixed with 1.6 mL of freshly prepared 0.2 mM DPPH (ethanol as solvent) solution, and reacted in the dark for 25 minutes. The same amount of MRS medium was used to replace the cell-free supernatant as the control group. OD after reaction 517 The value of the control group is A 0 , the experimental group is denoted as A 1 . Then the DPPH free radical scavenging rate is: (A 0 -A 1 ) / A 0 ×100%. Each group of experiments was performed in parallel three times.

[0031] The DPPH free radical scavenging rate of Weissella YM5S2 fusion was 44.65%...

Embodiment 3

[0036] antibiotic resistance

[0037] Antibiotic resistance was evaluated using the Kirby-Bauer disk diffusion method according to the testing standards of the American Clinical and Laboratory Standards Institute (CLSI). The drug susceptibility test results were divided into the following three levels: resistant (Resistant), intermediate (Intermediate) and sensitive (Susceptible). As shown in Table 1, the fusion Weissella YM5S2 is sensitive to most antibiotics, has no drug resistance, and has high safety.

[0038] Table 1. Antibiotic resistance of Weissella fusion YM5S2

[0039]

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PUM

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Abstract

The invention discloses Weissella confuse YM5S2 with antibacterial and antioxidant activity and an application thereof. The Weissella confusa YM5S2 is preserved in China General Microbiological Culture Collection Center (CGMCC) on January 07th, 2020 at the address of Institute of Microbiology, Chinese Academy of Sciences, No.3, Yard No.1, Beichen West Road, Chaoyang District, Beijing, and the accession number is CGMCC No:19308. The Weissella confusa YM5S2 with antibacterial and antioxidant activity can inhibit growth and propagation of enteric pathogenic bacteria such as salmonella typhimuriumand the like in vitro, has no hemolytic property, is sensitive to most antibiotics, has high safety and has high survival rate in gastric acid and intestinal high-bile salt environments.

Description

[0001] Technical field [0002] The invention belongs to the field of microorganisms, and in particular relates to a fusion Weissella strain with antibacterial and antioxidative activities and its application. Background technique [0003] Intestinal infection is one of the main factors threatening human intestinal health. Common intestinal pathogens include Escherichia coli, Staphylococcus aureus, Salmonella and so on. At present, antibiotics are commonly used to treat intestinal pathogenic bacterial infections, but the abuse of antibiotics has led to the increasingly serious problem of bacterial drug resistance. Studies have shown that probiotics play an important role in regulating the balance of intestinal flora, reducing the colonization of pathogenic bacteria, enhancing the immune function of the body, and helping to prevent and treat intestinal pathogenic bacterial infections. [0004] Most of the currently known probiotics are lactic acid bacteria. Common lactic acid...

Claims

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Application Information

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IPC IPC(8): C12N1/20A61K35/741A61P31/04A61P39/06C12R1/01
CPCA61K35/741A61P31/04A61P39/06C12R2001/01C12N1/205Y02A50/30
Inventor 储卫华王文倩谢巧根
Owner CHINA PHARM UNIV
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