50 results about "Specific lysis" patented technology

The invention relates to molecules inhibiting biologically active compounds and further comprising moieties specifically cleavable by a reagent produced by a target cell. The invention relates to inhibitors that bind, inhibit, suppress, neutralize, or decrease activity of a biologically active agent. Inhibitors comprise at least one moiety that bind, inhibit, suppress, neutralize, or decrease activity of a biologically active agent and at least one moiety that can be cleaved specifically by a reagent produced by target cells. The cleavage deactivates the inhibitor. Following cleavage, the active agent is liberated into the local environment. Administration of the inhibitor alone or together with the active agent suppress the compound's activity until it reaches the proximity of a target cell. Targeted specific release enables the agent concentration in specific site to reach levels that have desired therapeutic effects without systemic toxicity.

The invention relates to a reagent and a process for the identification and counting of biological cells in a sample. This reagent comprises a cell lysing agent selected from at least one detergent in a concentration capable of specifically lysing a given type of cells in the sample, and a stain capable of marking the intracellular nucleic acids of the remaining unlysed cells. Application in particular for the identification and counting of cells using an automated analysis system based on flow cytometry.

Methods and reagents are provided for specifically targeting biologically active compounds such as antiviral and antimicrobial drugs, or prodrugs containing the biologically active compound to specific sites such as specific organelles in phagocytic mammalian cells. The biologically active compound or prodrug is linked to a microparticle with a linker that is non-specifically or specifically cleaved inside a phagocytic mammalian cell. Alternatively, the biologically active compound or prodrug is impregnated into a porous microparticle or coated on a nonporous microparticle, and then coated with a coating material that is non-specifically or specifically degraded inside a phagocytic mammalian cell. The prodrug contains the biologically active compound linked to a polar lipid such as ceramide with a specific linker such as a peptide that is specifically cleaved to activate the prodrug in a phagocytic mammalian cell infected with a microorganism. A microparticle linked antimicrobial drug or prodrug may be used for killing a microorganism infecting a phagocytic mammalian cell in vivo or in vitro.

The invention discloses a phage for preventing and controlling soil-borne bacterial wilt, and an application thereof. The phage is a special Ralstonia solanacearum phage NN-742, and the special Ralstonia solanacearum phage NN-742 is preserved in China Center for Type Culture Collection on March 6, 2018 with the preservation number of CCTCC NO: M 2018102, and is named Podoviridae phage. The pathogen-specific phage can efficiently lyse bacteria to make the bacteria dead when used in biological control, and is nontoxic to the environment; and the phage has a strong specificity, does not destroy other normal florae, and can keep soil microbes balanced. The phage can specifically lyse bacterial wilt pathogens, and can be used to prevent and control tomato soil-borne bacterial wilt .

A recombinant adenovirus configurator deleted EIA coding sequence in human adenovirus type 5 genom and its application to diagnosing and treating tumor are disclosed. The site-directed mutation, PCR augmentation, enzyme severing, linking, subcloning, transfection, and single-cloning purification of recombinant adenovirus technologies, and the deleted EIA (382-1630 nt) sequence are used to screen the recombinant adenovirus configurator of being unable to express the EIA function protein. It can specifically kill tumor cells, but not normal cells, and has synergestic action to chemicotherapeutic medicines.

The invention discloses a lytic phage strain NJ-P3 capable of preventing and controlling tomato bacterial wilt, the classification name of the lytic phage strain NJ-P3 is Podoviridae phage, and the lytic phage strain NJ-P3 is preserved in the China Center for Type Culture Collection (CCTCC) on March 6, 2018, and is assigned with the accession number of CCTCC NO:M 2018099. The invention further discloses applications of the phage NJ-P3 in preventing and treating soil-borne bacterial wilt of tomato and applications of the phage NJ-P3 in preparing phage preparations for preventing and treating soil-borne bacterial wilt of tomato. The phage can realize specific splitting of pathogenic bacteria of bacterial wilt, when the temperature is 20-40 DEG C and the pH is 5-9, the phage has the highest activity and the strongest splitting capacity, the bactericidal ability is shown, the indoor antibacterial test shows that the antibacterial rate achieves 72.4%, and thus the phage can be used for preventing and treating soil-borne bacterial wilt of tomato. When the phage provided by the invention is used for preventing and treating soil-borne bacterial wilt of tomato, the disease prevention rate for the potted plant achieves 60%, and the biocontrol rate for the field achieves 85.19%.

The invention belongs to the technical field of molecular biology and particularly relates to a method for extracting DNA from a plant rich in polysaccharide and polyphenol. The provided method for extracting DNA from the plant rich in polysaccharide and polyphenol comprises the following steps: using a specific lysis solution to lyse a cell, extracting the DNA with Tris phenol-chloroform mixed solution, chloroform and a sodium perchlorate combination solution, and purifying the extracted DNA through a self-made adsorption column membrane, thereby obtaining the high-quality genomic DNA. The purity of the DNA extracted through the method for extracting DNA from the plant rich in polysaccharide and polyphenol is high, the impurity is low, the DNA is further prevented from being damaged by asuperoxide anion free radical or active oxygen, the completeness of the extracted genomic DNA is guaranteed, and the method is favorable for the scientific researches like construction of the gene library, PCR (Polymerase Chain Reaction) analysis, and Southern hybridization.

The invention discloses toxic Microcystis cracking cyanophage and a separation method and application thereof. The toxic Microcystis cracking cyanophage is characterized in that the cyanophage is a MaSSC-P strain, is classified and named as Microcystis aeruginosa single-stranded DNA cyanophage MaSSC-P, collected at the China General Microbiological Culture Collection Center on 25th May, 2015 and numbered as CGMCC No.10598. A purified cyanophage suspension is added into a Microcystis water sample, and the toxic Microcystis cracking cyanophage has specific cracking effect on Microcystis in the water sample and has cracking effect on Microcystis aeruginosa, Microcystis wesenbergii and Microcystis viridis. The toxic Microcystis cracking cyanophage has the advantage of being capable of safely, efficiently and quickly cracking toxic Microcystis in fresh water.

The Staphylococcus aureus bacteriophage phi11 endolysin has two peptidoglycan hydrolase domains (endopeptidase and amidase) and a SH3b cell wall-binding domain. In turbidity reduction assays, the purified protein can lyse untreated staphylococcal mastitis-causing pathogens, S. aureus and coagulase negative staphylococci (S. chronogenes, S. epidermis, S. hyicus, S. simulans, S. warneri, and S. xylocus), making it a strong antimicrobial protein and an effective candidate for treating multidrug-resistant staphylococci. Lytic activity is maintained at the pH (6.7) and the ‘free’ calcium concentration (3 mM) of milk. Truncated endolysin-derived proteins, containing just the endopeptidase domain, also lyse staphylococci, in the absence of the SH3b-binding domain.

A method is for prevention and/or biological control of wilt caused by Ralstonia solanacearum, by use of suitable bacteriophages. In addition a method uses the structural characterisation, genome sequence and activity of three specific lytic bacteriophages of R. solanacearum. Podovirus presents an elevated stability between 4° C. and 30° C. in an aqueous medium in the absence of a host. As a result of the high level of stability, lytic activity, elevated specificity towards R. solanacearum and the absence of activity against the microbiota associated with the plants to be protected, bacteriophages are used for the biological control of R. solanacearum in river courses and irrigation water, as well as in a method for preventing and/or controlling the wilt produced by the bacteria, in which at least one of the bacteriophages, or combinations thereof, are delivered to the plants and/or the soil in the irrigation water.

A mutant prourokinase plasminogen activator (M5) was developed to make prouPA less subject to spontaneous conversion to tcuPA in blood at therapeutic concentrations. Two-chain M5 was shown to form complexes with C1-inhibitor, which was the principal inhibitor of tcM5 in plasma. The effect of supplemental additions of C1-inhibitor on fibrinolysis and fibrinogenolysis by M5 was determined. Supplemental C1-inhibitor restored the stability of high-dose M5 and prevented fibrinogenolysis but not fibrinolysis, the rate of which was not compromised by the inhibitor. Due to higher dose tolerance of M5 in the presence of supplemental C1-inhibitor, the rate of fibrin-specific lysis reached that achievable by nonspecific fibrinolysis, which is the maximum possible for a plasminogen activator. Plasma C1-inhibitor stabilized M5 in plasma by inhibiting tcM5 which would otherwise greatly amplify non-specific plasminogen activation causing more tcM5 generation from M5. This unusual dissociation of inhibitory effects, whereby fibrinogenolysis and not fibrinolysis is inhibited, has significant implications for improving the safety and efficacy of fibrinolysis. Methods of reducing bleeding and non-specific plasminogen activation during fibrinolysis by administering M5 along with exogenous C1-inhibitor are disclosed.

The invention provides a vibrio campbellii phage capable of effectively lysing vibrio campbellii and application of the vibrio campbellii phage. The vibrio campbellii phage has a specific nucleotide sequence. The vibrio campbellii phage provided by the embodiments can specifically lyse the vibrio campbellii, the titer is high, so that vibrio campbellii disease can be effectively controlled in aquaculture after the vibrio campbellii phage is used alone or in combination.

The present invention relates to a milk-coagulating enzyme of bacterial origin and a process for producing cheese by using this enzyme, namely, an enzyme that is produced by a bacterium belonging to the genus Paenibacillus and exhibits milk-coagulating activity, characterized by having the following enzymological properties: 1) function: having an activity of coagulating milk to form a curd; 2) substrate specificity: acting on κ-casein as a substrate and specifically cleaving Thr94-Met95 in the presence of calcium; 3) optimum pH: 6.0-7.0, and 4) molecular weight: 35,000-37,000 Da when measured by SDS-PAGE; a process for producing the enzyme; a process for producing cheese; and a cheese-like food product.

The invention belongs to the technical field of biology, and relates to a phage isolate capable of specifically cracking Vibrio parahaemolyticus and application of the phage in the field of sea cucumber disease prevention, so that the economic benefit of sea cucumber breeding enterprises is improved, and food safety is guaranteed from the source. The vibrio parahaemolyticus phage vB_VpaP_VP-ABTNL-1 has a preservation number of CGMCC No.17991. The vibrio parahaemolyticus phage provided by the invention is applied to the prevention of the stichopus japonicus disease and can effectively prevent the infection of the vibrio parahaemolyticus in the breeding process.

A mutant prourokinase plasminogen activator (M5) was developed to make prouPA less subject to spontaneous activation during fibrinolysis. C1-inhibitor complexes with tcM5. The effect of C1-inhibitor on fibrinolysis and fibrinogenolysis by M5 was determined. Supplemental C1-inhibitor restores the stability of M5 but not that of prouPA. Clot lysis by M5 with supplemental C1-inhibitor showed no attenuation of the rate of fibrinolysis, whereas fibrinogenolysis was prevented by C1-inhibitor. Due to higher dose tolerance of M5 with C1-inhibitor, the rate of fibrin-specific lysis reached that achievable by nonspecific fibrinolysis without inhibitor. Plasma C1-inhibitor stabilized M5 in plasma by inhibiting tcM5 and thereby non-specific plasminogen activation. At the same time, fibrin-specific plasminogen activation remained unimpaired. This unusual dissociation of effects has significant implications for improving the safety and efficacy of fibrinolysis. Methods of reducing bleeding and non-specific plasminogen activation during fibrinolysis by administering M5 along with exogenous C1-inhibitor are disclosed.

The invention discloses a rice bacterial brown streak germ phage and an application thereof. The classification term of the phage is rice bacterial brown streak germ phage AP1 (Acidovorax avenae subsp.Avenae phage AP1), and the preservation number is CCTCC M 2014599. The application comprises an application of the rice bacterial brown streak germ phage in preparation of a phage preparation used for inhibiting rice bacterial brown streak germs, an application for inhibiting the rice bacterial brown streak germs and an application of preventing and treating the rice bacterial brown streak germs. The rice bacterial brown streak germ phage disclosed by the invention can specifically crack the rice bacterial brown streak germ, the burst size is about 160PFU/infected cells, and the cracking performance is stronger, so that the rice bacterial brown streak germ phage can be used for preventing and treating rice bacterial brown streak caused by the rice bacterial brown streak germs.

The present invention relates to a new cocktail of bacteriophages with specific lytic activity against Salmonella enteritidis, Salmonella typhimurium and Salmonella paratyphi B., for reducing, eliminating and/or preventing them in farm animals and animals from the poultry sector, such as poultry, hens and breeding hens, in addition to eggs. It may be administered as an additive in the feed, in water or by spray. Moreover, the cocktail may be used as a disinfectant in work areas of farms and abattoirs, and in processed foods, without affecting the organoleptic properties of the product.