Phage for preventing and controlling soil-borne bacterial wilt, and application thereof

A technology of phage and bacterial wilt, applied in the direction of virus/bacteriophage, phage, application, etc., can solve the problems of ignoring the biocontrol potential of bacteria and viruses

Active Publication Date: 2018-10-19
NANJING AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But the biocontrol potential of a much smaller but abundant population in the rhizosphere - bacteria and viruses - has been overlooked

Method used

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  • Phage for preventing and controlling soil-borne bacterial wilt, and application thereof
  • Phage for preventing and controlling soil-borne bacterial wilt, and application thereof
  • Phage for preventing and controlling soil-borne bacterial wilt, and application thereof

Examples

Experimental program
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Embodiment Construction

[0024] (1) Isolation and identification of phage

[0025] 1. Isolation and purification of phage

[0026] Host bacteria: Ralstonia solanacearum, QL-Rs1115 with strong pathogenicity (Wei et al.

[0027] Phage NN-P42 was screened from the rhizosphere soil of vegetable greenhouses in Nanning, Guangxi (108°21′E, 22°49′N) where tomato has been planted for a long time and the incidence of bacterial wilt is relatively serious. Its separation and purification process is as follows:

[0028] Medium:

[0029] NA liquid medium: glucose 10g, peptone 5g, yeast extract 3g, yeast extract 0.5g, deionized water 1000mL, adjust pH to 7.2-7.4, autoclave at 115°C for 30min.

[0030] NA solid medium: add 1.8% (g / 100ml) agar powder to NA liquid medium.

[0031] NA semi-solid medium: add 0.8% (g / 100ml) agar powder to NA liquid medium.

[0032] SM buffer: take MgSO 4 ·7H 2 O 2.0g, NaCl 5.8g, 1mol / L Tris-HCl (pH7.5) 50mL, 2% gelatin 5mL, add deionized water, dilute to 1000mL, autoclave at 121℃ f...

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Abstract

The invention discloses a phage for preventing and controlling soil-borne bacterial wilt, and an application thereof. The phage is a special Ralstonia solanacearum phage NN-742, and the special Ralstonia solanacearum phage NN-742 is preserved in China Center for Type Culture Collection on March 6, 2018 with the preservation number of CCTCC NO: M 2018102, and is named Podoviridae phage. The pathogen-specific phage can efficiently lyse bacteria to make the bacteria dead when used in biological control, and is nontoxic to the environment; and the phage has a strong specificity, does not destroy other normal florae, and can keep soil microbes balanced. The phage can specifically lyse bacterial wilt pathogens, and can be used to prevent and control tomato soil-borne bacterial wilt .

Description

technical field [0001] The invention belongs to an environment-friendly biocontrol agent, relates to a brachyphage NN-P42 and its application, and is specially used for preventing and treating soil-borne bacterial wilt. Background technique [0002] Tomato bacterial wilt is a bacterial vascular disease caused by Ralstonia solanacearum), which is devastating to Solanaceae crops. Ralstonia solanacearum has a wide range of hosts, strong environmental adaptability, large variation, and complex intraspecific inheritance. At present, bacterial wilt occurs in most parts of our country, but the incidence is serious in the south. Traditional control measures such as resistant varieties, grafting crop rotation, and chemical pesticides have problems in research and development, unstable effects, damage to soil ecology, and food safety. It is urgent to develop environmentally friendly control measures. [0003] The microbial community in the rhizosphere of plants has the "instinct" ab...

Claims

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Application Information

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IPC IPC(8): C12N7/00A01N63/00A01P1/00C12R1/92
CPCA01N63/00C12N7/00C12N2795/00021C12R2001/91C12N1/00
Inventor 韦中王孝芳王佳宁徐阳春沈其荣
Owner NANJING AGRICULTURAL UNIVERSITY
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