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50 results about "Oscillatoria" patented technology
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Oscillatoria is a genus of filamentous cyanobacterium which is named after the oscillation in its movement. Filaments in the colonies can slide back and forth against each other until the whole mass is reoriented to its light source. It is commonly found in watering-troughs waters, and is mainly blue-green or brown-green. Oscillatoria is an organism that reproduces by fragmentation. Oscillatoria forms long filaments of cells which can break into fragments called hormogonia. The hormogonia can grow into a new, longer filament. Breaks in the filament usually occur where dead cells (necridia) are present. Oscillatoria uses photosynthesis to survive and reproduce. Each filament of oscillatoria consists of trichome which is made up of rows of cells. The tip of the trichome oscillates like a pendulum. in reproduction, it takes place by vegetative means only. Usually the filament breaks into a number of fragments called homogonia. Each hormogonium consist of one or more cells and grow into a filament by cell division in one direction.
Enrichment, domestication and screening method and application of aerobic degradation microbial community of polycyclic aromatic hydrocarbon
InactiveCN101654657AGood oxygen degradation performanceTreatment using aerobic processesMicroorganismsVitamin CSorbent
The invention discloses an enrichment, domestication and screening method and application of an aerobic degradation microbial community of polycyclic aromatic hydrocarbon. An open glass bottle added with the polycyclic aromatic hydrocarbon and a basic medium is added with soil polluted by petroleum to enrich and domesticate aerobic degradation microbes of the polycyclic aromatic hydrocarbon; after30 days, a conical flask added with polycyclic aromatic hydrocarbon, a basic medium, trace liquid metal, vitamin c solution and an adsorbent is inoculated with the soil after the enrichment and domestication, and the soil is cultured in an oscillator at a temperature of between 25 and 30DEG C and a speed of 100r / min for 5 to 7 days, and then is subjected to circular transfer inoculation; and theaerobic degradation microbial community capable of highly efficiently removing the polycyclic aromatic hydrocarbon can be obtained after the circulation times is more than 8. The method can enrich, domesticate and screen the microbial community with good aerobic degradation performance on the polycyclic aromatic hydrocarbon, and aerobic degradation rates of the microbial community on naphthaline,phenanthrene, fluorine and pyrene are respectively 5.46mg.L<-1>.d<-1>, 0.48mg.L<-1>.d<-1>, 0.05mg.L<-1>.d<-1> and 0.06mg.L<-1>.d<-1>.
Owner:BEIJING NORMAL UNIVERSITY
Separation purification process and application of anaerobic degradation pure culture of polycyclic aromatic hydrocarbon
InactiveCN101195811AGood anaerobic degradation performanceBacteriaContaminated soil reclamationVitamin CPurification methods
Owner:BEIJING NORMAL UNIVERSITY
Bacillus polymyxa DYr4.4 with broad spectrum antibacterial activity and preparation method and application
ActiveCN108148793AHigh antibacterial activityHigh antagonistic activityBiocideBacteriaDiseaseCulture fluid
The invention discloses bacillus polymyxa DYr4.4 with broad spectrum antibacterial activity and a preparation method and application. The number is CCTCC M 2018023. The method comprises the steps thatA, a single bacterial colony of a bacterial strain is selected under the aseptic environment, inoculated to a culture solution and placed in a constant-temperature culture oscillator, the temperatureand rotation speed are adjusted, and oscillation culture is conducted; B, a fermentation solution is placed in each bottle, the bottles are placed in a large-amplitude constant-temperature freezing shaking table, and oscillation culture is conducted; C, the culture solution is prepared, wherein solutions are added to a tank in sequence, a tank body is sealed, the culture solution is heated and disinfected, an inoculation port is disinfected by igniting alcohol, 2 L of fermentation solution prepared in the last step is poured into the tank under the condition that the alcohol burns continuously, and the rotation speed of a stirrer is adjusted; D, the fermentation solution is filtered, an auxiliary material namely light calcium carbonate powder is added to the filtered fermentation solution, the mixed solution is placed in a centrifugal spray drying tower and dried, and dry powder is collected. The bacillus polymyxa DYr4.4 is applied to preparation of medicine for treating or preventingrust diseases of pear trees. Dry powder fermentation of the bacterial strain has the remarkable anti-disease effect on the rust diseases of the pear trees, the prevention effect reaches 67.09% or above, and leaf falling of the pear trees in advance is remarkably inhibited.
Owner:HUAZHONG AGRI UNIV
Process for the removal of calcium ions from the brine by marine cyanobacteria
InactiveUS6812011B2Economical simplePractical and convenientFungiBacteriaMicrobiologySynechocystis sp.
Owner:COUNCIL OF SCI & IND RES
Method for constructing artificial algal crusts by utilizing mixed algae
InactiveCN102613065ARapid multiplicationLarge biomassClimate change adaptationCultivating equipmentsNaviculaRefrigerated temperature
The invention discloses a method for constructing artificial algal crusts by utilizing mixed algae. The method comprises the following steps of: selecting sufficiently developed algal crusts on the desert, collecting the algal crusts, and grinding the algal crusts after air-drying the algal crusts; sieving bare sand with a 0.2mm sieve, then adding 1 liter of BG11 liquid culture medium to 100g of bare sand, and collecting the algae together with the grains of sand fixed on the algae three weeks later, wherein the inoculum size is 50g dry soil per liter of culture medium, the culture temperature is the room temperature, the illumination intensity is 4000lux, and in the cultured mixed algae, the dominant species are filamentous algae which are mainly microcoleus, lyngbya and phormidium and account for 80% of the total biomass, and species such as oscillatoria, chlorococcum humicola, chlorella, chlamydomonas, Navicula, hantzschia and the like account for 20% of the total biomass; grindingthe collected algae into alga powder after air-drying the collected algae, and putting the alga powder in a refrigerator at minus 20 DEG C; and carrying out inoculation on the bare sand when the soiltemperature is 20 DEG C, wherein the inoculum size is 80g.m<-2>, and the moisture applied daily is 3L.m<-2>. The method successfully avoids the problems of purification and separation of the algae, and has wide applicability.
Owner:XINJIANG INST OF ECOLOGY & GEOGRAPHY CHINESE ACAD OF SCI
Method of domesticating and screening benzene series anaerobic degradation flora
InactiveCN101016532AGood anaerobic degradation performancePigmenting treatmentBacteriaVitamin CTrace metal
The invention discloses a domestication screening method and appliance of benzene series anaerobic degraded flora in polluted soil and water biological degraded disposing technical domain, which comprises the following steps: adding electron acceptor, sodium sulfide and BTEX mixture into polluted soil; sealing and stewing at three months; adding basic culture medium, trace metal liquid and vitamin C solution into anaerobic box filled with nitrogen; domesticating soil; switching domestication soil into anaerobic bottle with mass ratio of water and soil in the anaerobic box; adding into BTEX, electron acceptor and sodium sulfide with mass ratio; culturing 7-9 days in rotating oscillator with the temperature at 20+-2 deg.c; circling at least 9 times; getting the degraded flora. This invention can screen good anaerobic degraded property microbe flora, which can make degraded speed range of BTEX at 1.6-3.5mg.L-1.d-1.
Owner:TSINGHUA UNIV
Separation and purification method and application of aerobic degradation pure culture of polycyclic aromatic hydrocarbon
InactiveCN101654656AGood oxygen degradation performanceTreatment using aerobic processesMicroorganismsVitamin CPurification methods
The invention discloses a separation and purification method and application of aerobic degradation pure culture of polycyclic aromatic hydrocarbon, which belongs to the technical field of biodegradation treatment on polluted soil and water. A bottom flat for an agar solid culture medium taking the polycyclic aromatic hydrocarbon as unique carbon source and energy is prepared in a bechtop, and isprovided with a top flat for the agar solid culture medium containing an aerobic degradation microbial community of the polycyclic aromatic hydrocarbon, and the culture medium flats prepared are cultured in an incubator for 10 to 15 days; in the bechtop, a conical flask added with the polycyclic aromatic hydrocarbon, a basic medium, trace liquid metal, vitamin c solution and an adsorbent is inoculated with the microbial community in the medium flat, and the microbial community is cultured in an oscillator at a temperature of between 25 and 30DEG C and a speed of 100r / min for 5 to 7 days, and then is subjected to circular transfer inoculation; and the aerobic degradation pure culture of the polycyclic aromatic hydrocarbon can be obtained after the circulation times is more than 8. The method can separate and purify to obtain the microbial pure culture with good aerobic degradation performance on the polycyclic aromatic hydrocarbon.
Owner:BEIJING NORMAL UNIVERSITY
Bacillus cereus strain CZBC1 capable of dissolving pond oscillatoria, and its application
ActiveCN103352010AThe effect of bacteriolysis is obviousApparent solubilityBacteriaMicroorganism based processesBacillus cereusMicrobiology
The invention discloses a Bacillus cereus strain CZBC1 capable of dissolving pond oscillatoria. The strain is Bacillus cereus, is preserved in China Center for Type Culture Collection in Wuhan, China on April 9, 2013 as Bacillus cereus CZBC1, and has a preservation number of CCTCC NO:M2013130. The Bacillus cereus strain CZBC1 has obvious dissolving and eliminating effects on a plurality of dominant oscillatoria in culture water. The invention also discloses an application of the Bacillus cereus strain CZBC1 capable of dissolving pond oscillatoria in the dissolving of the pond oscillatoria.
Owner:SOUTH CHINA SEA FISHERIES RES INST CHINESE ACAD OF FISHERY SCI
Stenotrophomonas maltophilia strain CZRST19 capable of dissolving pond oscillatoria, and its application
ActiveCN103352011AApparent solubilityEliminationBacteriaMicroorganism based processesStenotrophomonas maltophiliaMicrobiology
The invention discloses a monad strain CZRST19 capable of dissolving pond oscillatoria. The monad strain CZRST19 is preserved in China Center for Type Culture Collection in Wuhan, China on April 9, 2013 as Stenotrophomonas maltophilia strain, and has a preservation number of CCTCC NO:M2013129. The Stenotrophomonas maltophilia strain CZRST19 has obvious dissolving and eliminating effects on a plurality of dominant oscillatoria in culture water. The invention also discloses an application of the Stenotrophomonas maltophilia strain CZRST19 capable of dissolving pond oscillatoria in the dissolving of the pond oscillatoria.
Owner:SOUTH CHINA SEA FISHERIES RES INST CHINESE ACAD OF FISHERY SCI
Oxidoreductase activity testing method
The invention relates to the technical field of medicine biological detection, and provides a dehydrogenase activity detection method which can monitor oxidoreductase activity in real time in accordance with color change of a novel tetrazole monosulfonate detection reagent. A tetrazole monosulfonate detection system is adopted, and a good linear dose relationship is presented in detection of dehydrogenase activity; and an EC50 curve is obtained in accordance with an absorbance value of a reactant at 450nm, and dehydrogenase activity evaluation is implemented. The dehydrogenase activity detection method provided by the invention is highly sensitive and is convenient to operate; and the detection method is broad in application range, covering dehydrogenase of various organisms, including archaebacteria (extreme thermophilic bacteria and extreme halophilic bacteria), bacteria (lactobacillus, nitrifying bacteria, escherichia coli, diplococcus pneumoniae and the like), various cellular structure bio-actinomycetes, cyanobacteria (oscillatoria, chroococcus, nostoc and the like), most unicellular algae (chlamydomonas, green algae and the like), fungi (edible fungi, saccharomycetes, mould and the like) as well as animals and plants.
Owner:HANGZHOU JENNIFER BIOTECH CO LTD
Preparation method of black fungus apple vinegar
InactiveCN104726315AIncrease productionSmall footprintVinegar preparationNutritive valuesBiotechnology
The invention discloses a preparation method of black fungus apple vinegar, which comprises the following steps: 1. inoculating a black fungus strain in a basal culture medium, and culturing at 28 DEG C for 7 days; 2. inoculating the activated strain into a sterilized liquid culture medium, standing at room temperature for 24 hours, putting into an oscillator, and culturing at 30 DEG C at the rate of 150 rpm for 8 days; 3. carrying out wall-breaking homogenization on the strain subjected to amplification culture twice, blending with apple juice subjected to heating sterilization, inoculating yeast to perform alcohol fermentation, and inoculating vinegar bacteria to perform acetic fermentation; and 4. after the fermentation finishes, filtering to obtain the black fungus apple vinegar. By utilizing the submerged fermentation technique, the method has the advantages of high productive capacity, small occupied area, short period and high controllability. The obtained product ingeniously combines the health care functions of the black fungus and fruit vinegar, has abundant nutrients and good taste, has the functions of nourishing and strengthening the body, enhancing the immunity and the like, and has higher nutritive value.
Owner:QINGDAO XIUXIAN FOODS
Preparation method of ammonia nitrogen adsorbent by utilizing southern magnolia and application of adsorbent
InactiveCN101745368APretreatment is simple and easyImprove adsorption efficiencyOther chemical processesWater/sewage treatment by sorptionSorbentSewage treatment
The invention discloses a preparation method of an ammonia nitrogen adsorbent by utilizing southern magnolia and the application for removing ammonia nitrogen in water body; southern magnolia leaves are used as raw material, and then 30-100 meshes of the adsorbent is obtained through collection, washing, airing, drying, crushing and sieving of the raw material; the adsorbent is mixed with ammonia nitrogen solution according to a certain proportion, the mixture is oscillated for 20 hours on an oscillator, so as to effectively remove the ammonia nitrogen in the water body. In the invention, the waste is reutilized, the raw material cost is low, the preparation and adsorption process are simple, and the method is suitable for sewage treatment of ammonia nitrogen with middle-low concentration.
Owner:INST OF SOIL SCI CHINESE ACAD OF SCI
Glycolipid fraction from cyanobacteria for treatment of diseases of the oral cavity
Owner:BLUEGREEN BIOTECH
Glycolipid Mixture with Anti-Inflammatory Activity Obtained from Oscillatoria Planktothrix
It was observed that a highly purified preparation of polar glycolipids extracted from cells of the cyanobacterium Oscillatoria Planktothrix is characterized by the presence of at least one species of high molecular weight glycolipid comprising one or more units of rhamnose. In such mixture, having a level of nucleic acid contamination lower than (or equal to) 3%, an inhibitory activity toward ATP synthase (ATP-SX) was identified which is capable of decreasing the level of extracellular ATP and thereby the extent of the inflammatory response. The glycolipid mixture is especially useful in inflammation induced by ischemic stimuli, cancer or autoimmune diseases (such as multiple sclerosis, inflammatory bowel disease (Crohn's disease, ulcerative colitis), psoriasis, rheumatoid arthritis, diabetes, autoimmune thyroiditis, systemic lupus erythematosus), in addition to systemic inflammatory states such as systemic inflammatory syndrome (SIRS), sepsis, vasculitis, or in localized inflammatory states, such as neurodegenerative diseases or asthma.
Owner:BLUEGREEN BIOTECH
Method for preparing ammonia nitrogen sorbent by utilizing abandoned strawberry leaves and application of sorbent
InactiveCN101733077APretreatment is simple and easyHigh adsorption rateOther chemical processesWater contaminantsSpecific adsorptionSorbent
Owner:INST OF SOIL SCI CHINESE ACAD OF SCI
Industrial liquid-solid combined fermentation method for bacillus cereus preparation for dissolving oscillatoria in aquaculture pond
InactiveCN105087426AGuaranteed purityLow cost effectBacteriaMicroorganism based processesBacillus cereusOscillatoria
The invention discloses an industrial liquid-solid combined fermentation method for bacillus cereus preparation for dissolving oscillatoria in an aquaculture pond. According to the invention, industrial liquid fermentation and solid fermentation systems of a microbial inoculum are taken as the platform, a production technology process including seed solution culturing, liquid-solid combined fermentation of thalli, drying and smashing of the fermented product, and packaging to obtain the finished product is established, a system solution for the industrial liquid-solid combined fermentation production of the bacillus cereus preparation for dissolving oscillatoria is provided, and the relevant microbial inoculum product is formed. According to the method, the characteristics of high efficiency and high purity and the liquid fermentation method are reserved, the low-cost effect of the solid fermentation method is also considered, and thus the production cost of the microbial inoculum is reduced; meanwhile, the influences on the algae-lytic characteristic and other ecological characteristics of the bacillus cereus preparation caused by the industrial scale are avoided, and the microbial inoculum product can be applied to the aquaculture pond for preventing and controlling cyanobacterial bloom.
Owner:SOUTH CHINA SEA FISHERIES RES INST CHINESE ACAD OF FISHERY SCI
Microbial water purifying agent for weever farming water and preparation method of microbial water purifying agent
InactiveCN104278020ASuitable for online processingEasy to settleMicroorganism based processesOn/in inorganic carrierBiotechnologyLeucothrix
The invention discloses a microbial water purifying agent for weever farming wastewater and a preparation method of the microbial water purifying agent. The preparation method comprises the following steps: adding 40-60 parts by weight of magnetic silica beads with surface modification of C8 and C18 alkyl chains into 200-300 parts by weight of water; adding 200-300 parts by weight of a microbial reagent comprising 50-80 parts of oscillatoria, 40-80 parts of rhodomicrobium, 40-60 parts of nitrosococcus, 30-50 parts of nitrospina, 30-40 parts of crenothrix, 30-40 parts of pseudomonas aeruginosa, 3-6 parts of leucothrix, 3-6 parts of beggiatoa, 40-60 parts of zooflagellates, 40-60 parts of suctorians, 40-50 parts of dunaliella and 40-50 parts of blue-green algae; and further adding 80-100 parts by weight of a beef extract peptone agar culture medium for culture. By putting the microbial water purifying agent disclosed by the invention into a weever farming pond, the purpose of purifying the water in the farming pond can be achieved.
Owner:SHANGHAI KUIYA ENVIRONMENTAL PROTECTION TECH
Glycolipid fraction from cyanobacteria for treatment of diseases of the oral cavity
The present invention relates to the preparation and use of a glycolipid fraction from Oscillatoria Planktothrix sp., for the treatment and / or prevention of bacterial gum diseases primarily caused by: Actinobacillum actinomycetemconcomitans, Porphyromonas gingivalis, Tannerella forsythia, Treponema denticola and even more preferably by Porphyromonas gingivalis. Said gum diseases, in particular gingivitis and periodontitis (pyorrhea), are primarily caused by a pro-inflammatory response to components of P. gingivalis, leading to destruction of periodontal tissue, and are often accompanied by osteoclastogenesis (increased number of osteoclasts responsible for destruction of bone tissue), and by chronic infection.
Owner:BLUEGREEN BIOTECH
Fermentation method of alternaria tenuissima
InactiveCN107988084ARealize industrial productionFungiMicroorganism based processesFungicidePetri dish
The invention discloses a Fermentation method of alternaria tenuissima. The fermentation method comprises the following steps: taking out an alternaria tenuissima culture HZ-1 stored at 4 DEG C; picking up part of bacterial colony and inoculating the same to a fresh PDA culture medium to be cultured, then picking up part of the bacterial colony from the culture medium and inoculating the same to afresh PDA culture medium, and repeating the step 2-3 times to obtain an activated HZ-1 culture which is vigorous in growing power; taking the HZ-1 culture cultured for 7d in a petri dish, perforating8mm bacterial cakes by a perforator, and inoculating the bacterial cakes 10 / L into the PDA liquid culture medium; and culturing the cakes in an oscillator, and filtering the mixture to obtain a HZ-1fermenting liquid. According to the fermentation method, the culture HZ-1 is reproduced massively, thereby laying a theoretical foundation for industrial production and further popularization and application of a biological fungicide.
Owner:QINGHAI UNIVERSITY
A Stenotrophomonas maltophilia strain czrst19 for dissolving Oscillatoria in ponds and its application
ActiveCN103352011BEliminationStrong specificityBacteriaMicroorganism based processesStenotrophomonas maltophiliaMicrobiology
Owner:SOUTH CHINA SEA FISHERIES RES INST CHINESE ACAD OF FISHERY SCI
Photosynthetic type microbial preparation for aquaculture
InactiveCN105884046AImprove survival rateDisinfect thoroughlyWaste water treatment from animal husbandryBiological water/sewage treatmentWater qualityAnabaena
The invention discloses a photosynthetic type microbial preparation for aquaculture. The photosynthetic type microbial preparation for aquaculture is prepared from the following components in parts by weight: 8-10 parts of chroococcoid, 6-8 parts of oscillatoria, 6-8 parts of nostoc, 5-7 parts of anabaena, 4-5 parts of spiral seaweeds, 1-2 parts of rhodospirillum, 1-2 parts of rhodop seudanonas palustris and 1-2 parts of red micro bacteria. In this way, the photosynthetic type microbial preparation for aquaculture has the advantages of being complete in disinfection, cutting off viral infection, purifying water quality, achieving harmless standards, being natural and free of toxicity, increasing an aquaculture survival rate, and the like, and has a wide market prospect on popularization of the photosynthetic type microbial preparation for aquaculture.
Owner:苏州市相城区阳澄湖镇东枪塘水产养殖专业合作社
Method for simultaneously purifying and detecting avian leukosis and avian salmonellosis by utilizing one hatching egg and application of method
The invention belongs to the field of animal husbandry and veterinary and in particular relates to a method for simultaneously purifying and detecting avian leukosis and avian salmonellosis by utilizing one hatching egg and application of the method. The method comprises the following steps: feeding breeder flocks in single cages and encoding; collecting primary hatching eggs of the breeder flocks; enabling the primary hatching eggs to correspond to numbers of the hatching eggs one by one; disinfecting the surfaces of the hatching eggs and opening egg shells from hatching egg air chamber ends;sucking egg white and yolks and putting into PE (Polyethylene) tubes respectively and marking; putting egg white and yolk samples into a refrigerator and freezing and thawing for a plurality of times; taking the frozen and thawed egg white; detecting an ALV virus antigen through an avian leukosis ELISA (Enzyme Linked Immunosorbent Assay) detection method; taking the frozen and thawed yolks and adding normal saline and chloroform; after uniformly mixing on an oscillator, centrifuging and layering a solution, wherein a lower layer is an organic solvent, a middle layer is denatured lipid proteinand an upper layer is an extracted antibody; and sucking the antibody of the upper layer and detecting a salmonellosis antibody through a plate agglutination method; and eliminating chicken flock individuals with positive detection results of the ALV virus antigen and the salmonellosis antibody.
Owner:INST OF ANIMAL SCI & VETERINARY HUBEI ACADEMY OF AGRI SCI
Method for extracting streptomyces alga dissolution active substances
InactiveCN108048490AImprove securityNo secondary pollutionMicroorganismsMicroorganism based processesOperabilityMicrocystis
The invention provides a method for extracting streptomyces alga dissolution active substances, and belongs to the field of eutrophic water body bioremediation. An isometric absolute ethyl alcohol isused for repeatedly extracting streptomyces fermentation liquor filtrate, after centrifugalization is completed, a rotary evaporator is used for concentrating an organic phase to be dry, crude substances are obtained, then methyl alcohol is used for dissolving the crude substances, and silica gel column chromatography separation is carried out; the components with efficient alga dissolution activity are subjected to high-performance liquid chromatographic separation, and the alga dissolution active substances are obtained. Adopted streptomyces comes from an eutrophic water body, the alga dissolution active substances secreted by the streptomyces are secondary metabolites, high safety is achieved, and no secondary pollution is caused to the water body. The alga dissolution active substancescan be used for treatment of the eutrophic water body caused by typical harmful algae such as microcystis, anabaena, aphanizomenon and oscillatoria. The extraction method is simple in process, high in operability, low in production cost, good in alga dissolution effect and suitable for large-scale production.
Owner:无锡市拜沃特环保科技有限公司 +1
Industrial multistage communicated liquid fermentation method for bacillus cereus preparation for dissolving oscillatoria in pond
InactiveCN105039209ARealize fermentation productionFree from pollutionBacteriaMicroorganism based processesSludgePrawn
The invention discloses an industrial multistage communicated liquid fermentation method for a bacillus cereus preparation for dissolving oscillatoria in a pond. The industrial multistage communicated liquid fermentation method is characterized in that by taking an inocula industrial liquid fermentation system as a platform, and taking the guarantee of growth performance and algae-lytic characteristic of algicidal bacteria preparation products as an objective, a production process flow of products, including seed liquid culture, multistage communicated fermentation tank culture, bacterial sludge preparation as well as carrier compounding, drying, crushing and packaging, is established, a system solution for industrial liquid fermentative production of the bacillus cereus preparation for dissolving the oscillatoria is provided, and relevant inocula products are formed. According to the method, the demand of the inocula products on industrial liquid fermentative production can be met; on the other hand, the condition that the algae-lytic characteristic and the growth performance of the bacillus cereus preparation products formed by the method disclosed by the invention are influenced by industrial and scale production is avoided; the bacillus cereus preparation products still have good specificity of dissolving the oscillatoria, have no dissolving effect on high-quality green alga and diatom and no adverse effect on culture of prawns, and can be applied to culture production.
Owner:SOUTH CHINA SEA FISHERIES RES INST CHINESE ACAD OF FISHERY SCI
Method for domesticating and screening degrading flora for petroleum hydrocarbon from seawater
The invention discloses a method for domesticating and screening a degrading flora for petroleum hydrocarbon from sweater, which belongs to the technical field of the biological degradation treatment of the seawater polluted by spilled oil, which comprises the following steps: adding the seawater into an open glass bottle into which crude oil, a basic culture medium, a trace metal liquid and a vitamin c solution are added, carrying out the enrichment and the domestication of the degrading flora for the petroleum hydrocarbon for 10-12 months, and then, introducing an enriched and domesticated seawater sample into a conical flask into which the crude oil, the basic culture medium, the trace metal liquid and the vitamin c solution are added; culturing the seawater sample for 6-8 days in an oscillator at a temperature of 10-15 DEG C and a rotational speed of 100 r / min, and then, carrying out circular transfer, so as to obtain the degrading flora for efficiently removing the petroleum hydrocarbon in the seawater polluted by the spilled oil after circulation times are more than 6 times.
Owner:BEIJING NORMAL UNIVERSITY
A kind of microbial water purifying agent for black fish culture water and preparation method thereof
ActiveCN104276665BSuitable for online processingEasy to settleBiological water/sewage treatmentSynechococcusThiothrix
Owner:贺兰县晶诚水产养殖有限公司
Application of fumaric acid in inhibiting growth of blue-green algae
InactiveCN113016802AWide variety of sourcesLow priceBiocideAnimal repellantsAcetic acidEcological safety
The invention provides application of the fumaric acid in inhibiting the growth of the blue-green algae. In a blue-green algae water area with a certain concentration, the growth of the blue-green algae can be inhibited for a long time by applying 40mg / L fumaric acid. Compared with common small molecule acid inhibitors (acetic acid and hydrochloric acid), the inhibitor has the advantages of good inhibition effect, no secondary pollution and high ecological safety. In addition, fumaric acid not only can inhibit the water bloom dominant species microcystis aeruginosa, but also has an inhibiting effect on oscillatoria, and has general applicability to blue-green algae. Fumaric acid can effectively control blue-green algae for a long time, and is low in cost, environment-friendly and suitable for being popularized to the society.
Owner:NANJING UNIV +1
Microbial water clarifying agent for Chinese softshell turtle cultivation water and preparation method thereof
ActiveCN104276663AEasy to settleImprove purification efficiencyBiological water/sewage treatmentThiothrixParamecium sp.
The invention discloses a microbial water clarifying agent for Chinese softshell turtle cultivation water and a preparation method thereof. The preparation method comprises the following steps: adding 20-40 parts by weight of magnetic silica spheres with C8 and C18 alkyl chain-modified surfaces into 200-400 parts by weight of water; adding 80-100 parts by weight of microbial reagents, wherein the microbial reagents comprise the following raw materials: 50-70 parts of rhodopseudomonas palustris, 50-70 parts of oscillatoria cyanobacteria, 10-30 parts of nitrosospira, 10-30 parts of nitrosolobus, 10-20 parts of thiobacillus dentrificans, 10-20 parts of leptothrix, 1-3 parts of thiothrix, 1-3 parts of beggiatoaceae, 30-70 parts of paramecium, 30-70 parts of ciliate, 20-40 parts of platymonas subcordiformis and 20-40 parts of blue algae; and then adding 40-60 parts of by weight of beef extract peptone agar medium for culturing. The microbial water clarifying agent can achieve the purpose of purifying the cultivation water after being added into a Chinese softshell turtle culture pond.
Owner:上海乾界生物科技有限公司
Method for extracting flavonoid substances in penthorum chinense pursh
The invention discloses a method for extracting flavonoid substances in penthorum chinense pursh. The method comprises the steps that (1), the penthorum chinense pursh is smashed into fine powder withthe particle size of being 40 mesh or above; (2), the fine powder is dried for 4-6 hours at the temperature of 60-80 DEG C; (3), a methyl alcohol solution is added, a nanometer metal material is added, micro waves are applied, after uniform mixing, water-bath extraction is conducted for 1.5 hours at the extraction temperature of 60-80 DEG C by using a refluxing device, and then the mixture is taken out and subjected to vacuum filtration to obtain filter liquor; (4), pretreated polyamide resin and macroporous resin are weighed, the filter liquor obtained in the step 3 is added to obtain a mixture, the mixture is placed into an oscillator for oscillation for 12 hours at the temperature of 30 DEG C, elution is conducted by using a solvent, an eluant is collected, concentrated and evaporatedto dryness, and then the extracts are obtained.
Owner:SICHUAN UNIV
Method for increasing egg laying amount of arma chinensis
The invention relates to a method for increasing the egg laying amount of arma chinensis. The method comprises the following steps that 1) newly laid arma chinensis eggs are placed in a centrifuge tube; 2) after an ethanol solution is added into the centrifuge tube in step 1), and the centrifuge tube is placed in an oscillator for three-time oscillation treatment; 3) after a formaldehyde solutionis added into the centrifuge tube in step 2), the centrifuge tube is placed in the oscillator for oscillation treatment; 4) after the ethanol solution is added into the centrifuge tube in step 3), thecentrifuge tube is placed in the oscillator for oscillation treatment; 5) after the obtained arma chinensis eggs in step 4) are rinsed thoroughly with a sterilized deionized water, the surface moisture is sucked dry with sterilization filter paper. The method for increasing the egg laying amount of arma chinensis has the advantages that after the arma chinensis eggs treated through the experimental steps are hatched into adult arma chinensis, under the conventional breeding conditions of a laboratory, the egg laying amount is effectively increased, thereby being conductive to the expanding propagation of arma chinensis population, and saving the economics, time and labor cost of arma chinensis raising.
Owner:INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI
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