Industrial liquid-solid combined fermentation method for bacillus cereus preparation for dissolving oscillatoria in aquaculture pond

Abstract

The invention discloses an industrial liquid-solid combined fermentation method for bacillus cereus preparation for dissolving oscillatoria in an aquaculture pond. According to the invention, industrial liquid fermentation and solid fermentation systems of a microbial inoculum are taken as the platform, a production technology process including seed solution culturing, liquid-solid combined fermentation of thalli, drying and smashing of the fermented product, and packaging to obtain the finished product is established, a system solution for the industrial liquid-solid combined fermentation production of the bacillus cereus preparation for dissolving oscillatoria is provided, and the relevant microbial inoculum product is formed. According to the method, the characteristics of high efficiency and high purity and the liquid fermentation method are reserved, the low-cost effect of the solid fermentation method is also considered, and thus the production cost of the microbial inoculum is reduced; meanwhile, the influences on the algae-lytic characteristic and other ecological characteristics of the bacillus cereus preparation caused by the industrial scale are avoided, and the microbial inoculum product can be applied to the aquaculture pond for preventing and controlling cyanobacterial bloom.

Description

technical field [0001] The invention belongs to the technical field of microbial preparations, and in particular relates to an industrialized liquid-solid composite fermentation method for dissolving Bacillus cereus preparations for cultivating Oscillator algae in ponds. Background technique [0002] With the rapid development of the aquaculture industry, the eutrophication of the aquaculture water body is increasing day by day, leading to a large number of blue-green algae blooms and high-frequency outbreaks of blue-green algae blooms (Cao Yucheng et al., 2007). Cyanobacteria consume a large amount of oxygen and can release cyanobacterial toxins, which deteriorate water quality, cause poisoning and death of aquatic animals, and have a huge impact on the water environment and breeding benefits (Peng Congcong et al., 2011). Studies have shown that the use of alginolytic bacteria can specifically inhibit harmful microalgae and achieve the effect of optimizing the microalgal co...

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Problems solved by technology

Although some scholars have reported on the fermentation process of Bacillus cereus and the optimization of fermentation conditions of alginolytic bacteria (Wang Haiyun et al., a strain of Bacillus cereus and its preparation method and application, application number 201410223612.7; Zhu Jie et al. Solid fermentation and application of algal bacteria, application number 201210308126.6; Sun Mei et al., a method and application of Bacillus megaterium and its solid fermentation preparation bacterial agent, application number 201310413693.2; Rong Xiaojun et al., A kind of Bacillus cereus and its Preparation and application, application number 201010602326.3; Ye Jiangyu et al., Research on optimization of fermentation conditions of algicidal bacteria by response surface method, 2011; The above-mentioned Bacillus cereus, and not all strains of Bacillus cereus have algicidal properties. In addition, there are many reports on the fermentation of alginolytic bacteria, most of which are of research nature, and have not yet met the needs of industrialized fermentation production of bacterial agents. , and there is no industrial production product of related bacterial agents

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