50 results about "Synechocystis sp." patented technology

This invention relates to new use of marine cyanobacteria lyngbya, Oscillatoria, Sprulina, Anabaena and Synechocystis deposited with ATCC having accession numbers ATCC PTA-4602 and 4603 for the removal of calcium ions from sea-brine and sub-soil brine having a density range 10 to 25.5° Be', by culturing the cyanobacteria, inoculating the cyanobacteria culture to raw brine of 10 to 25.5° Be', filtering the resultant mixture to obtain a brine having less calcium, and to separate the cyanobacteria which can be reused if desired.

The traditional column chromatography process for purifying recombinant proteins has high production costs and cumbersome steps. Non-chromatographic purification methods based on affinity tags can reduce costs and increase target protein yields, but the cost of tag removal is high. In order to solve the above problems, the present invention proposes a simple and efficient purification method that only needs centrifugation and cracking steps. The method uses CipA inclusion body protein and intein protein (Synechocystis sp. PCC6803 DnaB, Ssp DnaB), and utilizes the self-assembly function of CipA and the self-cleavage function of DnaB under weak acid conditions to realize protein purification. The target protein can be any protein to be expressed and purified, and the purity after purification can reach more than 95%. In addition, the present invention also optimizes the connecting peptide between CipA and DnaB, which further improves the cleavage efficiency of DnaB and reduces the influence of CipA on the activity of the target protein. This method has the potential to be applied to the industrial production of enzymes. Key words: protein purification method, inclusion body protein CipA, self-cleavage protein DnaB.

The invention discloses a synechococcus engineering bacterium and a preparation method and application thereof. The synechococcus engineering bacterium is a cesA gene-deficient Synechococcus sp. PCC 7002 mutant; the plasmid or genome of the mutant is integrated with 7 cellulose synthesis related genes (cmcase, ccp, acsA, acsB, acsC, acsD and bglxA) of Gluconacetobacter xylinum ATCC 53582; and the7 genes are started and expressed by a promoter PcpcBA of a cpcBA gene of Synechococcus sp. PCC 7002 or Synechocystis sp. PCC6803. The cellulose content of the engineering bacterium accounts for about 13% of the dry weight of the cell wall; and simultaneously, the cellulose produced by the synechococcus engineering bacterium has a short growth cycle and does not have the pollution problem of lignin in wood, the extraction and purification processes can be greatly simplified, and the degree of pollution to the environment can also be greatly reduced correspondingly, thus the synechococcus engineering bacterium is an ideal biological engineering bacterium for producing cellulose and is further used as a raw material for producing biological ethanol.