37results about How to "Increase Lutein Content" patented technology

The invention discloses a yellow cartfish feedstuff which comprises animal protein, plant protein, fat source, minerals and vitamin additives, wherein the minerals include the salts of Cu, Fe, Mn and Zn, characterized in that, calculated by the content of dried solid (by weight percent), the feed contains 5-10% of maize protein meal.

The invention discloses silkworm feed. The silkworm feed comprises the following components in weight part: 50-65 parts of mulberry leaves, 10-12 parts of dandelion leaves, 12-21 parts of vegetable leaves, 7-11 parts of corn gluten meal, 3-5 parts of multivitamins, 1-3 parts of carotene, and 0.5-0.8 parts of a mildew preventive. The mulberry leaves are used as a main raw material. Various vegetable leaves are added to achieve complete nutrition. The dandelion leaves can clear away heat and toxic materials and resist against viruses. The multivitamins and the carotene are essential nutrient substances for organisms. The silkworm feed can improve immunity, meet requirements for growth of the silkworm, and reduce diseases.

The invention provides a hydroxytyrosol composition used for protecting eyesight. The composition is characterized in that the composition is prepared by preparing an active component by mixing hydroxytyrosol, hyaluronic acid, phytoxanthin, proanthocyanidins and taurine according to a certain ratio, and then preparing oral preparations with the active component and a proper amount of common auxiliary materials. The composition has functions of improving eyesight, and improving eye and visual functions. The composition is used for eyesight protection, asthenopia improvement, prevention and adjuvant therapy for macular degeneration, macular degenerative diseases, and diabetic retinopathy, optic atrophy, and optic nerve degeneration, and the like.

The invention discloses a tagetes erecta particles production method capable of increasing xanthophyll content, which belongs to the field of natural pigment processing. According to the method, the picked fresh tagetes erecta is subjected to two-time fermentation by using a lactic acid bacteria diluent, physical extruding, drying and dehydrating, and water cooling, and then the material is subjected to crushing granulation under inert gas protection to obtain the tagetes erecta particles. A plate and frame filter as well as membrane filtration are used in order for re-covering residual tagetes erecta and xanthophyll in waste water after physical extruding, and the xanthophyll content during tagetes erecta processing is increased. The xanthophyll content in the produced tagetes erecta particles is high, and the waste water can be better processed.

The invention relates to application of a sll0659 gene to synthesis of synechocystis carotenoids. The nucleotide sequence of the sll0659 gene is shown in SEQ ID No.1. The invention provides application of the sll0659 gene in synechocystis PCC6803, which has an important effect on synthesis of synechocystis PCC6803 beta-carotene. By knocking out the sll0659 gene in synechocystis PCC6803, the lutein content of the synechocystis PCC6803 is increased by 22.3%, zeaxanthin content is increased by 31.3%, the echinenone content is reduced by 34.3%, and the percentage content of beta-carotene is reduced by 1.3%. Meanwhile, HPLC detection results show when synechocystis PCC6803 involved in the invention is compared with wild synechocystis PCC6803, an obvious peak exists in the carotenoid components in the sll0659 gene knockout mutant, and the obvious peak is determined to be alpha-carotene by contrasting the obvious peak with a standard product and detecting.

The invention relates to the technical field of aquatic animal feed and particularly relates to a feed for culturing pelteobagrus fulvidraco. The feed is prepared from the following raw materials in parts by weight: 30-50 parts of fish meal, 60-80 parts of vital gluten, 50-55 parts of corn flour, 5-10 parts of shrimp shell meal, 10-15 parts of blood meal, 0.02-0.025 part of yeast powder, 3-5 parts of barbed skullcap herb, 10-20 parts of stringy stonecrop herb, 2-5 parts of Japanese milkwort herb, 1.1-1.14 parts of tangerine peels, 12-15 parts of whey powder, 0.03-0.04 part of phytase, 0.2-0.25 part of mountain ebony barks, 0.1-0.3 part of Chinese tallow leaves and 1-1.1 parts of compound vitamin. By virtue of the feed, the intake of pelteobagrus fulvidraco can be improved; the non-specific immunity and anti-oxidization capability of pelteobagrus fulvidraco are enhanced; the content of total carotenoids and the content of xanthophyll in back and belly skins of fish bodies are improved, and the body color of pelteobagrus fulvidraco can be deepened.

The invention discloses a preparation method of a high-yielding lutein transgenic chlorella. The preparation method aims to transfer an IPP (Isopentenyldiphosphate) isomerase encoding gene to an objective chlorella to obtain a transgenic chlorella, wherein the lutein yield of the transgenic chlorella is higher than that of the objective chlorella; and the amino acid sequence of the IPP isomerase is the sequence 2 in the sequence table. Tests show that, the method provided by the invention, can transfer an IPP (Isopentenyldiphosphate) isomerase encoding gene expression box to a wild-type chlorella to obtain the transgenic chlorella of which lutein content is increased by 30.95 percent maximumly compared with the wild-type chlorella and the lutein yield is increased by 36.77 percent maximumly compared with the wild-type chlorella; and the transgenic chlorella is the high-yielding lutein transgenic chlorella.

The invention discloses a feed additive for laying hens, capable of improving egg quality and prepared by comprehensively utilizing coconut shells. The feed additive is prepared from the following raw materials in parts by weight: 20-23 parts of Chinese chestnuts, 11-14 parts of Chinese flowering quinces, 9-12 parts of laver, 5-6 parts of kaempferiae, 10-15 parts of fresh milk, 15-17 parts of tangerine peel, 9-12 parts of cloves, 6-7 parts of licorice roots, an appropriate quantity of coconut shells, an appropriate quantity of lactic acid bacteria, an appropriate amount of hydrochloric acid of 0.2mol / L, an appropriate amount of ethanol of 60%, an appropriate amount of acetone, and an appropriate amount of water. The feed additive disclosed by the invention can improve the quality of eggs, can improve the production performance of the laying hens, and has the efficacies of immunity resistance and disease resistance.

The invention discloses a broiler chicken feed additive capable of promoting growth and prepared by comprehensively utilizing coconut shells. The feed additive is prepared from the following raw materials in parts by weight: 25-28 parts of red grapes, 10-15 parts of gordon euryale seed powder, 12-14 parts of sunflower seeds, 14-17 parts of ulva lactuca, 4-5 parts of cushaw petals, 15-17 parts of orange peel, 9-12 parts of cloves, 6-7 parts of licorice roots, an appropriate quantity of coconut shells, an appropriate quantity of lactic acid bacteria, an appropriate amount of activated dry yeast, an appropriate amount of 0.2mol / L hydrochloric acid, an appropriate amount of 60% ethanol, an appropriate amount of acetone and an appropriate amount of water. The feed additive disclosed by the invention can promote the broiler chickens to eat, the growth speed of the broiler chickens is high, the fattening effect is obvious, the meat quality of the broiler chickens is sweet and fragrant, and the meat of the broiler chickens is rich in various nutrient values.

The invention discloses a laying hen feed additive capable of improving stress ability and prepared by comprehensively utilizing coconut shells. The feed additive is prepared from the following raw materials in parts by weight: 30-34 parts of water chestnuts, 9-12 parts of shiitake mushrooms, 1-2 parts of grape seed oil, 10-12 parts of coconut juice, 15-17 parts of orange peel, 9-12 parts of cloves, 6-7 parts of licorice roots, an appropriate quantity of coconut shells, an appropriate quantity of lactic acid bacteria, an appropriate amount of 0.2mol / L hydrochloric acid, an appropriate amount of 60% ethanol, an appropriate amount of acetone, an appropriate amount of soybean isoflavone and an appropriate amount of water. Through the adoption of the feed additive disclosed by the invention, the stress ability of the laying hens can be improved, and the production efficiency of the laying hens can be improved.

The invention discloses a preparation method of a high-yielding lutein transgenic chlorella. The preparation method aims to transfer an IPP (Isopentenyldiphosphate) isomerase encoding gene to an objective chlorella to obtain a transgenic chlorella, wherein the lutein yield of the transgenic chlorella is higher than that of the objective chlorella; and the amino acid sequence of the IPP isomerase is the sequence 2 in the sequence table. Tests show that, the method provided by the invention, can transfer an IPP (Isopentenyldiphosphate) isomerase encoding gene expression box to a wild-type chlorella to obtain the transgenic chlorella of which lutein content is increased by 30.95 percent maximumly compared with the wild-type chlorella and the lutein yield is increased by 36.77 percent maximumly compared with the wild-type chlorella; and the transgenic chlorella is the high-yielding lutein transgenic chlorella.

The invention provides a method used for extracting and separating xanthophyll in marigold. The method comprises following steps: firstly, enzyme is adopted for hydrolysis of marigold flower powder soas to obtain an enzymatic hydrolysate; the enzymatic hydrolysate is subjected to salting out extraction; an obtained product is allowed to stand or is subjected to centrifugation phase separation soas to obtain an upper organic solvent phase and a lower inorganic salt water phase, and the upper organic solvent phase is subjected to evaporation so as to obtain xanthophylls. Compared with conventional extraction method, the advantages are that: the advantages of enzyme-salting out extraction and enzyme-microwave auxiliary salting out extraction are adopted, direct extraction of xanthophyll infresh marigold flowers is realized, steps of fresh marigold flower fermentation, granulation pretreatment process are avoided; and in addition, salting out extraction is capable of realizing phase separation, so that extraction and primary extraction on xanthophyll are realized, primary purifying of target products is realized, enzyme and microwave action is capable of increasing effective component extraction yield effectively, simplifying production technology, and shortening production period.

The invention discloses an introduction and cultivation technique of pigment marigold in a high altitude area, in particular an introduction and cultivation technique for an area at an altitude between 1,700 and 2,200 meters. The method comprises the steps of pretreatment of seeds of pigment marigold, seedling raising, transplanting, management and harvesting. The method adopts physical and chemical method to pretreat the seeds to achieve a germination percentage of the seeds of 90 percent and a uniform seedling raising means to provide high quality seedlings for large scale planting. By planting pigment marigold in a high altitude area, the yield of fresh flowers and content of xanthophylls in the fresh flower are greatly improved. The cultivation method, compared with the prior method, contributes to construction of standard pigment marigold plantations, carrying out standard planting demonstration of pigment marigold and improving the technological content of the pigment marigold, enables farmers to learn planting technologies of pigment marigold as quickly as possible and obtain high quality pigment marigold products, and greatly improves economic benefits.

The invention provides a hydroxytyrosol composition used for protecting eyesight. The composition is characterized in that the composition is prepared by preparing an active component by mixing hydroxytyrosol, hyaluronic acid, phytoxanthin, proanthocyanidins and taurine according to a certain ratio, and then preparing oral preparations with the active component and a proper amount of common auxiliary materials. The composition has functions of improving eyesight, and improving eye and visual functions. The composition is used for eyesight protection, asthenopia improvement, prevention and adjuvant therapy for macular degeneration, macular degenerative diseases, and diabetic retinopathy, optic atrophy, and optic nerve degeneration, and the like.

The invention relates to application of a sll0659 gene to synthesis of synechocystis carotenoids. The nucleotide sequence of the sll0659 gene is shown in SEQ ID No.1. The invention provides application of the sll0659 gene in synechocystis PCC6803, which has an important effect on synthesis of synechocystis PCC6803 beta-carotene. By knocking out the sll0659 gene in synechocystis PCC6803, the lutein content of the synechocystis PCC6803 is increased by 22.3%, zeaxanthin content is increased by 31.3%, the echinenone content is reduced by 34.3%, and the percentage content of beta-carotene is reduced by 1.3%. Meanwhile, HPLC detection results show when synechocystis PCC6803 involved in the invention is compared with wild synechocystis PCC6803, an obvious peak exists in the carotenoid components in the sll0659 gene knockout mutant, and the obvious peak is determined to be alpha-carotene by contrasting the obvious peak with a standard product and detecting.

A method and equipment for cultivating chlorella capable of increasing lutein production. The method includes 1) an initial heterotrophic step, adding a heterotrophic culture solution with a pH of 6 to 8 in a fermenter, and inoculating chlorella in the fermentation tank. In the tank, the culture temperature is 27-32°C, the pH is controlled in the range of 6-8, the DO value is controlled in the range of 20%-40%, and the algae cell density is 5-50×106 / mL; 2) Autotrophic-heterotrophic In the nutrient cycle stage, the initial heterotrophic culture solution obtained in step 2) is transferred to autotrophic culture, the culture temperature is 27-32°C, the carbon dioxide ventilation rate is 0.03-0.05VVM, and the DO value is also controlled in the range of 20%-40%. Cultivate until the increase in lutein content is less than 1.5-3% / h, transfer to a fermenter to continue heterotrophy, after 18-36 hours of heterotrophy, then transfer to the autotrophic culture stage, such an autotrophic-heterotrophic cycle.

The invention relates to a preparation method of lutein microcapsules and a lutein microcapsule instant beverage, and belongs to the field of foods. According to the lutein microcapsules, lutein is used as a core material, sodium caseinate is used as a wall material, the embedding rate of lutein in the microcapsules is as high as 97%, the dissolving speed of the microcapsules in water is 44 s, the quality of the microcapsules is good, raw materials are food-grade foods or are from foods, and are non-toxic and high in safety, the microcapsules can be eaten, and the digestibility and the absorptivity of the lutein microcapsules are better than those of natural lutein. According to the lutein microcapsule instant beverage disclosed by the invention, the lutein microcapsules, yellow corn grits and apricot kernels are used as main raw materials, and steviol glycoside is used as sweetening agent. The beverage is good in mouth feel, the content of main nutrient substances including zeaxanthin and lutein is also very high, only a small quantity of the beverage needs to be brewed every day, and the intake 10mg / day of the lutein by a healthy adult, suggested by WHO / FAO, can be met; and the beverage contains coarse food grains namely corn and a non-cane sugar sweetening agent, so that the beverage is the unique choice of health-care foods for middle-aged people, old people and patients suffering from diabetes.