41 results about "Chromoprotein" patented technology

An object of the present invention is to provide a red or orange fluorescent protein, which is characterized in that the difference (stokes shift) between an excitation peak value (wavelength of maximum absorption) and a fluorescence peak value (wavelength of maximum fluorescence) is greatened, so that the maximum fluorescence can be obtained by the maximum excitation. The present invention provides a novel fluorescent protein monomerized by introducing a mutation into a florescent protein derived from Fungia sp., and a novel chromoprotein and fluorescent protein derived from Montipora. sp.

The present invention provides nucleic acid molecules encoding a fluorescent and chromo-proteins and mutants, variants and derivatives thereof, as well as proteins and peptides encoded by these nucleic acids. The nucleic acid molecules and proteins of interest are isolated from non-Aequorea Hydrozoa species. The proteins of interest include yellow fluorescent protein, phiYFP, from Phialidium sp., green fluorescent protein hydr1GFP and purple chromoprotein, hm2CP from hydroid medusae of sub-order Anthomedusae. Also of interest are proteins that are substantially similar to, or derivatives, or homologues, or mutants of, the above-referenced specific proteins. Also provided are fragments of the nucleic acids and the peptides encoded thereby, as well as antibodies specific to the proteins and peptides of the invention. In addition, host-cells, stable cell lines and transgenic organisms comprising above-referenced nucleic acid molecules are provided. The subject protein and nucleic acid compositions find use in a variety of different applications and methods, particularly for labeling of biomolecules, cell or cell organelles. Finally, kits for use in such methods and applications are provided.

The invention relates to a silk reeling method for GFP familial chromoprotein silkworm colorful cocoons, wherein cocoon is dried at a temperature of less than 70 DEG C, and obtained dried cocoons are cooked for 30 minutes in a solution containing 0.5 percent of sodium silicate and 0.1 percent of sodium hydroxide at a temperature of 42 DEG C, placed in mild water at a temperature of between 50 and 55 DEG C, and subjected to cocoon brushing and general subsequent technologies such as filament guiding, twisting and ensheathing, cross winding, coiling, drying and silk rereeling to complete the process of silk reeling. The invention skillfully breaks through the technical bottleneck of large-scale silk reeling of the GFP familial chromoprotein silkworm colorful cocoons by utilization of temperature control and change of the chemical compositions of the cocoon cooking solution, establishes a reliable method which is suitable for performing large-scale silk reeling on the GFP familial chromoprotein silkworm colorful cocoons, and provides the novel method for actual practicality of the GFP familial chromoprotein silkworm colorful cocoon varieties.

The invention particularly discloses an inclusion compound for improving the photostability of chromoprotein and application of the inclusion compound. The inclusion compound is prepared from an inclusion formed by at least one polyphenol molecule or partial groups of the polyphenol molecule in a cyclodextrin cavity; a polyphenol-cyclodextrin inclusion can improve the photostability of the chromoprotein, thereby helping the chromoprotein keep natural luster and activity; a protecting effect of the polyphenol-cyclodextrin inclusion is remarkably superior to a direction effect of polyphenol, and adverse influence of direct contact between the polyphenol and the chromoprotein can be reduced; the polyphenol-cyclodextrin inclusion can be mixed with the chromoprotein; sugar or sugar alcohol also can be added as an accessory in a mixing process and then a mixture is applied to a formula of food or cosmetics. According to the inclusion compound disclosed by the invention, pretreatment of the chromoprotein is avoided, and the structure of the chromoprotein is not changed, so that photodegradation of the chromoprotein is remarkably reduced, accurate luster of the chromoprotein also can be maintained, and important realistic significance for application of the chromoprotein in the cosmetics and the food is realized.

The invention belongs to the field of protein engineering, in particular to a method for separating and preparing medical phycocyanin from alga. The method comprises the steps of processing alga plants by an ultrasonic assistant extraction technology at low temperature, extracting alga protein by a protein extraction buffer solution to obtain a coarse extract; then adjusting pH of the coarse extract to be acidic, standing, centrifuging clear liquid and taking supernatant and adjusting pH to be neutral; or adjusting pH of the coarse extract to be acidic, performing membrane filtration after standing to obtain a filtrate; and subjecting the filtrate to hydroxyapatite column chromatography, wherein the moving phase is the protein extraction buffer solution, collecting eluting peak of phycocyanin, and then carrying out spray-drying to obtain the medical phycocyanin. The method for separating and preparing the medical phycocyanin from the alga provided by the invention overcomes the deficiency that the yield is low and the cost is high in the prior art for extracting the phycocyanin. The alga chromoprotein composite with the phycocyanin purity of A620 / A280 of more than 2.0 and the recover rate of the phycocyanin of more than 25% is obtained for the first time, and therefore, the method for separating and preparing the medical phycocyanin from the alga provided by the invention simplifies the separating steps, and is simple to operate and reduces the cost. The property of the phycocyanin is stable.

The invention discloses a bacillus gene traceless knockout / knockin plasmid and method, and a kit. The genome of the bacillus gene traceless knockout / knockin plasmid contains only one kind of resistance gene marked with a positive selection marker, a replicon capable of being duplicated in escherichia coli, a thermo-sensitive type replicon capable of being duplicated in bacillus, at least one I-Scel enzyme cutting site and only one kind of chromogenic protein gene, wherein promoters in front of the resistance gene and the chromogenic protein gene are both constitutive promoters in bacillus. The invention further provides a helper plasmid used for improving the knockout / knockin efficiency of the bacillus gene traceless knockout / knockin plasmid, and resistance gene and chromogenic protein gene in the genome of the helper plasmid are different from those of the knockout / knockin plasmid. By the adoption of the two plasmids, one or more target DNA sequences in the genome of bacillus can be modified continuously or iteratively, and the plasmids can be applied to multiple fields including bacillus genetic modification, metabolic process researching, functional genome researching and industrial application researching.

The invention discloses a method for separating and purifying sulodexide crude drugs from a heparin by-product, and relates to the technical field of medicine. The method for separating and purifyingthe sulodexide crude drugs from the heparin by-product comprises the steps that a heparin sulfate and dermatan sulfate crude product is obtained after the heparin by-product is dissolved by adopting high-temperature precipitation separation; and then, a crude product solution is oxidized, precipitated and dried through a hydrogen peroxide-ozone system to obtain sulodexide. According to the methodfor separating and purifying the sulodexide crude drugs from the heparin by-product, the hydrogen peroxide-ozone system is adopted during oxidation, and the oxidation effect can be improved to effectively remove chromoprotein and impurities. During precipitation, compared with a traditional low-temperature precipitation method, a high-temperature precipitation method is adopted, the titer recoveryrate is increased by 10%, using of a centrifugal machine and ion exchange resin is further reduced, compared with traditional hydrogen peroxide oxidation treatment, 60% time is shortened, the productquality and production efficiency are improved, the method for separating and purifying the sulodexide crude drugs from the heparin by-product is suitable for large-scale industrial production, the period is short, the cost is low, the heparin by-product is sufficiently and effectively used, the product purity is higher, and the stability is higher.

The invention discloses a phathopoiesis related gene (photosensitive pigment-protein gene) from Xanthomonas causing cabbage black rot. The photosensitive pigment-protein gene provided by the invention contains one of the following nucleotide sequences: 1) DNA sequence shown in SEQ ID No.1, 2) DNA sequence containing over 80% homology with DNA sequence shown in SEQ ID No. 1. The invention also involves the application of the genes in plant disease prevention and cure, as well as the application for drug target in plant disease prevention and cure.

Disclosed is a denatured spirulina of being removed the unpleasant smell of spirulina, of maintaining its nutrients undestroyed, of being changed the unpleasant deep blue color from light blue to rose pink, and of showing higher suspensible nature to cold water, characterized in that spirulina is suspended in distilled or purified water to destroy its cell membranes by osmolytic treatment, so the chromoprotein phycocyanin leaks out of the cell membranes; chromoprotein in the spirulina is partially denatured by heating the resulting mixture; condensing, condensing under reduced pressure or finally freeze-dried the resulting mixture.

The invention discloses a preparation method of high-purity spirulina blue. The preparation method is characterized by adopting spirulina powder as a raw material, and performing continuous productionincluding extraction, filtration, salting out, desalination concentration, micro-encapsulation, spray drying and mixing to obtain spirulina blue, which is blue powder. Phycocyanin is dark blue powder, is separated from spirulina, and looks beautiful. The main component of spirulina blue is phycocyanin, which belongs to porphyrin cyanophycin and is one of infrequent chromoproteins in the nature. The phycocyanin has bright color, is protein itself with abundant nutrients, has complete amino acid composition, and is high in amino acid content. The preparation method is short in production periodand doesn't cause secondary pollution. The obtained spirulina blue is high in purity, contains 71.3% of phycocyanin, has the purity A620 / A280 reach 3.6, and can meet the requirement of food and cosmetic industry.

The invention discloses a compound for improving photostability of chromoproteins and an application thereof. The compound is a mixture comprising amino acids and vitamin C, wherein the amino acids are any one types from acidic amino acids, neutral amino acids, alkaline amino acids and amino acid derivatives, and the vitamin C is one or more of vitamin C and vitamin C salt. When the amino acid-vitamin C mixture is compounded with a chromoprotein, saccharides or sugar alcohol is added as auxiliary materials. The compound can eliminate adverse influences of the amino acids on the chromoproteins and provide a photo-protective effect on the chromoproteins, which is significantly better than single application of vitamin C, thereby maintaining the natural color and activity of the chromoproteins. The compound is free of pretreatment on the chromoproteins and does not change the structure of the chromoproteins, so that the compound has important practical significance in application of the chromoproteins in cosmetics and foods.

The invention discloses a reagent for coloration of protein in gel electrophoresis as well as a using method and application. The reagent comprises a first component and a second component, wherein the first component is the solution formed by dissolving the compound with isothiocyanic acid groups into a solvent for protecting the isothiocyanic acid groups; the second component is the alkaline loading buffer which does not contain amino groups; the using method comprises the steps as follows: mixing the first component and the second component to form treating fluid and mixing the treating fluid with a to-be-tested protein sample, or mixing the second component with the to-be-tested protein sample and then adding the first component, and heating to form the chromoprotein sample solution. According to the invention, the reagent can be used in gel electrophoresis, and has the advantages of simplicity and easiness in use, reliability in effect, low usage amount, easiness in production and processing, wide application range, no pollution, time conservation and labor conversation, capabilities of performing real-time observation and being used for pre-colorating of the protein in gel electrophoresis, and the like, and a protein electrophoresis pattern has no changes which can be observed.

The invention discloses an application of an LHAP1 protein and its coding gene in the regulation of plant photosynthesis. The transmembrane protein LHAP1 localized in chloroplast is found, and it is found that the LHAP1 deletion mutant plant (lhap1) of the LHAP1 gene has a yellow-green leaf color and a reduced photosynthetic efficiency. The regulation effect of the LHAP1 in the transport process of the plant light-capturing chromoprotein LHCII is determined through the separation of the LHAP1 and the identification and analysis of gene functions, so the content of the LHCII protein can be regulated by using the gene actual production through a transgenic means.

The present invention provides methods for production and purification of active chromoproteins produced by Actinomadura sp. 21G792. The chromoproteins are useful for developing pharmaceutical compositions and treating diseases such as cancer or bacterial infections.

The present invention relates to the preparation of adsorbent, and is especially porous chitosan-amino acid copolymer ball as the macromolecular chromoprotein adsorbing medium. The present invention has the advantages including opening one way of introducing ¿CCOOH into chitosan microballoon, and synthesizing one kind of porous copolymer ball with high adsorption capacity on macromolecular chromoprotein.

Five protein families cooperate to form the warhead structure that characterizes enediyne compounds, both chromoprotein enediynes and non-chromoprotein enediynes. The protein families include a polyketide synthase and thioesterase protein which form a polyketide synthase catalytic complex involved in warhead formation in enediynes. Genes encoding a member of each of the five protein families are found in all enediyne biosynthetic loci. The genes and proteins may be used in genetic engineering applications to design new enediyne compounds and in methods to identify new enediyne biosynthetic loci.

The invention discloses a construction method of an adeno-associated virus expression vector, and the method comprises the following steps: obtaining a first gene sequence and a second gene sequence, the first gene sequence comprising a nuclear localization protein gene sequence, a chromogenic protein gene sequence and a receptor protein gene sequence; introducing an enzyme digestion site into the first gene sequence, and performing enzyme digestion treatment on the first gene sequence and the second gene sequence; wherein the second gene sequence carries a restriction enzyme cutting site; and connecting the first gene sequence and the second gene sequence after enzyme digestion treatment to obtain the adeno-associated virus expression vector. The invention also discloses a preparation method of the adeno-associated virus, a cell marking method and an expression vector. In this way, marking and positioning of whole-brain specific type neurons in a specific brain region can be achieved.

The present application provides a chromoprotein (shCP), comprising amino acid sequences with greater than 96% consistency of SEQ ID NO: 1. The chromoprotein is derived from Stichodactyla haddoni and has an absorption spectrum of 350˜650 nm. The present application also provides a nucleic acid sequence, comprising a nucleic acid sequence encoding the amino acid sequence of the shCP. The present application further provides a vector, comprising the nucleic acid sequence of the shCP. The present application still provides a host, including the vector carried with the nucleic acid sequence of the shCP.

The invention discloses an application of an LHAP1 protein and its coding gene in the regulation of plant photosynthesis. The transmembrane protein LHAP1 localized in chloroplast is found, and it is found that the LHAP1 deletion mutant plant (lhap1) of the LHAP1 gene has a yellow-green leaf color and a reduced photosynthetic efficiency. The regulation effect of the LHAP1 in the transport process of the plant light-capturing chromoprotein LHCII is determined through the separation of the LHAP1 and the identification and analysis of gene functions, so the content of the LHCII protein can be regulated by using the gene actual production through a transgenic means.

The invention belongs to the field of biological medicaments, and relates to a protection effect of a light-harvesting chromoprotein, namely, phycocyanin of algae on lens epithelial cells. The phycocyanin can obviously inhibit the reduction of the survival ratio of the lens epithelial cells and the increment of the content of reactive oxygen species (ROS) and malonaldehyde (MDA) caused by sodium selenite, and also can obviously inhibit the structure damage and the cell apoptosis of the lens epithelial cells caused by the sodium selenite; and therefore, the phycocyanin has a remarkable protection effect on the lens epithelial cells.

The invention discloses a whole-cell biosensor for detecting p-nitrophenol and a detection method, the whole-cell biosensor for detecting p-nitrophenol comprises a host cell and a pPNP-mrfp plasmid or a pPNP-ami1CP plasmid located in the host cell, the nucleotide sequence of the pPNP-mrfp plasmid is shown as SEQ ID NO: 1, the nucleotide sequence of the pPNP-ami1CP plasmid is shown as SEQ ID NO: 2, and the nucleotide sequence of the pPNP-mrfp plasmid or the nucleotide sequence of the pPNP-ami1CP plasmid is shown as SEQ ID NO: 3. The nucleotide sequence of the pPNP-amilCP plasmid is as shown in SEQ ID NO: 2, and the physical map of the pPNP-mrfp plasmid and the physical map of the pPNP-amilCP plasmid are as shown in figure 1. When the whole-cell biosensor is used for detecting extracted paranitrophenol in soil, the combination of paranitrophenol and repressor protein pobR eliminates the combination of pobR and manipulating gene locus (pobO), reverse fluorescent protein or chromoprotein genes are transcribed to generate fluorescent protein or chromoprotein, and by detecting the fluorescence intensity value or absorbance value, the content of paranitrophenol in the soil can be detected. The rapid and high-throughput detection of the extracted p-nitrophenol in the soil is realized.