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Compound for improving photostability of chromoproteins and application thereof

A pigment protein and photostability technology, which is applied in applications, medical preparations containing active ingredients, food science, etc., can solve the problems that stabilization technologies and methods need to be developed and cannot meet the requirements of phycocyanin photostability, etc. achieve the effect of eliminating adverse effects

Active Publication Date: 2017-07-14
上海鲜可生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the shelf life of food and cosmetics is as long as 2-3 years. The existing technology shows the effect of vitamin C alone. After 6 months of shelf life simulation experiments, only about 10% of phycocyanin remains, indicating that vitamin C alone still cannot meet the needs of cosmetics. and the requirements for the photostability of phycocyanin in the food field, more stabilization techniques and methods are yet to be developed

Method used

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  • Compound for improving photostability of chromoproteins and application thereof
  • Compound for improving photostability of chromoproteins and application thereof
  • Compound for improving photostability of chromoproteins and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Example 1: Compound preparation and comparative test for improving the photostability of phycocyanin (see the appendix figure 1 )

[0040] 1. Experimental platform

[0041] Xenon lamp aging instrument, the wavelength range is 290nm-800nm, the experimental condition is 340nm, the irradiation intensity is 0.35W / m2, the blackboard temperature is 45℃, and the light irradiation is 6h, which can simulate the shelf life of 6 months. Visually observe color and appearance, and detect absorbance at 620nm.

[0042] 2. Light protection effect

[0043] Phycocyanin 400ppm, dissolved in deionized water, placed at 4°C, protected from light, as a control, standard sample 1;

[0044] Phycocyanin 400ppm, dissolved in deionized water, subjected to xenon lamp aging, is sample 2;

[0045] Phycocyanin 400ppm, vitamin C 100ppm, dissolved in deionized water, subjected to xenon lamp aging, sample 3;

[0046] Phycocyanin 400ppm, arginine 100ppm, dissolved in deionized water, subjected to xen...

Embodiment 2

[0060] Example 2: Preparation of a compound for improving the photostability of phycocyanin

[0061] Weigh 5g of glycine, 1g of vitamin C, 0.6g of phycocyanin, and 92g of xylooligosaccharide, dissolve in 1L of deionized water, stir to dissolve, pre-freeze at -45°C, and sublimate under reduced pressure at 20°C to obtain a mixed freeze-dried powder. The mixture can be applied in any formulation of food, cosmetics, providing active or blue shade. It is prepared into an aqueous solution containing 400 ppm of phycocyanin, and according to the xenon lamp aging method of the present invention, the residual rate of phycocyanin is 35%.

Embodiment 3

[0062] Example 3: Preparation of a compound for improving the photostability of phycocyanin

[0063] Weigh 1 g of glycine, 1 g of sodium ascorbate, 2 g of phycocyanin, and 96 g of trehalose, dissolve in 1 L of deionized water, stir to dissolve, pre-freeze at -45°C, and sublimate under reduced pressure at 20°C to obtain a mixed freeze-dried powder. The mixture can be applied in any formulation of food, cosmetics, providing active or blue shade. It is prepared into an aqueous solution containing 400 ppm of phycocyanin, and according to the xenon lamp aging method of the present invention, the residual rate of phycocyanin is 39%.

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Abstract

The invention discloses a compound for improving photostability of chromoproteins and an application thereof. The compound is a mixture comprising amino acids and vitamin C, wherein the amino acids are any one types from acidic amino acids, neutral amino acids, alkaline amino acids and amino acid derivatives, and the vitamin C is one or more of vitamin C and vitamin C salt. When the amino acid-vitamin C mixture is compounded with a chromoprotein, saccharides or sugar alcohol is added as auxiliary materials. The compound can eliminate adverse influences of the amino acids on the chromoproteins and provide a photo-protective effect on the chromoproteins, which is significantly better than single application of vitamin C, thereby maintaining the natural color and activity of the chromoproteins. The compound is free of pretreatment on the chromoproteins and does not change the structure of the chromoproteins, so that the compound has important practical significance in application of the chromoproteins in cosmetics and foods.

Description

technical field [0001] The invention mainly relates to the technical field of cosmetics and food, in particular, the invention relates to the technical field of improving the photostability of pigment protein. Background technique [0002] Due to its physiological activity, safety and natural color, natural active pigments have good application prospects in cosmetics, food, health care products and other fields. However, natural active pigments generally face the problem of poor stability, especially a type of pigment protein: that is, pigments composed of chromophores and protein subunits. Due to the combination with proteins, their stability is even worse. Phycocyanin and phycoerythrin are two typical pigment proteins. [0003] Phycocyanin is mainly present in cyanobacteria, red algae and cryptophyta, and is one of the light-harvesting pigments for photosynthesis of these algae, especially in spirulina with a content as high as 10%-20% (Hao Wei, Pan Ling. Research progre...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K8/67A61K8/44A61K8/64A61K8/60A61Q19/00A23L33/175A23L33/15A23L29/30A23L5/41
CPCA23V2002/00A61K8/44A61K8/447A61K8/60A61K8/645A61K8/676A61K2800/43A61Q19/00A23V2250/708A23V2200/048A23V2250/0616A23V2250/0606A23V2250/0622A23V2250/0618
Inventor 侯沛卢玲巧
Owner 上海鲜可生物技术有限公司
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