76 results about "Fungia" patented technology

The invention relates to Agrobacterium mediated transformation of moulds comprising species of the fungal sub-divisions Ascomycotina, Basidiomycotina, Deuteromycotina, Mastigomycotina, and Zygomycotina.Examples demonstrate the transformation of Aspergillus awamori (both protoplasts and conidia), Aspergillus nidulans, Aspergillus niger, Colletotrichum gloeosporioides, Fusarium solani pisi, Neurospora crassa, Trichoderma reesei, Pleurotus ostreatus and Agaricus bisporus (all conidia), and Fusarium graminearum (both conidia and rehydrated freeze dried ATCC material).Especially for Aspergillus awamori the transformation frequency is much higher than with conventional mould transformation techniques.It has further been found that not only one expressable gene can be introduced into these moulds, but even multiple copies of such gene, which, moreover, can be targeted e.g. in the chromosomal pyrG locus, as exemplified for A. awamori. These multiple copies can be of a gene encoding a desired, homologous or heterologous, protein.

This invention relates to a method of using walnut shell as matrix for mushroom cultivation. Walnut shells contain vegetable tannin that inhibit reproduction of true fungus, so ignore possibility of it as mushroom medium. This method includes medium heat sterilization, vaccination. Medium preparation methods are : (1) smash the walnut shell, in size of diameter below 1 mm to 10mm, at room temperature water immersion walnut shells 24-72 hours to reduce tannin content, walnut shells and water quality ratio (1 : 2) - (1:8); (2) take walnut shell, wood and wheat bran as compost, quality ration of: the walnut shell 50% -70%, 10% -38% sawdust, wheat bran 18% -10%, by adding glucose 0.5% -1% and Gypsum 1%. This invention use abandoned walnut shells as materials, not rely on higher-value raw materials, will brought facilitating role for walnut producing farmers, walnut processing enterprises and mushroom cultivation.

The present invention is based on a plurality of benefits from the extracts of mycelia of individual fungal species, and mixtures of species, to provide an armamentarium of defenses from multiple stressors in order to help bees survive a complex of symptoms collectively called colony collapse disorder (CCD). More particularly, the present invention utilizes specific concentrations of extracts from pure cultured mycelium from mushroom forming fungi to reduce harmful viruses in bees and to increase the longevity of bees.

The present invention discloses one kind of low molecular Brazil mushroom polysaccharide and its preparation process and application in antagonizing tumor metastasis. The low molecular Brazil mushroom polysaccharide extracted from the sporophore of edible fungus Brazil mushroom has homogeneous glucosan in molecular weight of 48,000 as the main component. It is prepared with sporophore of Brazil mushroom, and through crushing, leaching in distilled water, precipitating in alcohol to obtain coarse Brazil mushroom polysaccharide product, deproteinizing, chromatographic separation in DEAE650M cellulose column, gradient eluting with NaCl solution, chromatographic separation of eluted liquid in Toyopearl HW-65F column, eluting with NaCl solution, chromatographic separation of eluted matter in Toyopearl HW-50S column, eluting with NaCl solution, and drying.

The invention discloses a method for cultivating wood log cultivated ganoderma lucidum spore powder with rich selenium, pertaining to the field of cultivating edible (medicinal) fungi. The method mainly includes: mixing the inorganic selenium, ganoderma lucidum fungi and ganoderma lucidum culture medium according to certain proportion, through biotransformation of ganoderma lucidum fungi, improving the content of organic selenium in spore powder. The steps for cultivating wood log cultivated ganoderma lucidum spore powder with rich selenium are as follows: adding sodium selenite containing inorganic selenium for cultivating ganoderma lucidum fungi with rich selenium, adding sodium selenite for preparing ganoderma lucidum culture medium with rich selenium, inoculating ganoderma lucidum fungi, inorganic selenium entering the interior of the wood logs through ganoderma lucidum mycelium to realize biotransformation, cultivating ganoderma lucidum with rich selenium and ganoderma lucidum spore powder with rich selenium. The content of the organic selenium in the spore powder cultivated by this method can reach to 51mg / kg-130mg / kg. After people take the spore powder, the level of blood selenium and immunity can be improved greatly, and the spore powder has good effect of illness treatment and health care.

The invention relates to a method for promoting growth of sweet sorghum in a saline-alkali land with arbuscular mycorrhizal fungi and an organic fertilizer, sweet sorghum seeds are sown in the saline-alkali land, the arbuscular mycorrhizal fungi are inoculated and the organic fertilizer is applied simultaneously, and conventional field management is performed in the growing process of the sweet sorghum until harvesting. According to the method, the buffering performance and adsorption capacity of the soil can be enhanced by the aid of the actions of adsorption, complexing, reduction, volatilizing and the like of the organic fertilizer, the toxic action of saline ions is reduced, and the plant growth is promoted; the effectiveness of the saline ions is reduced by the aid of the chelation, the hypha immobilization function and the like of the arbuscular mycorrhizal fungi, the plant tolerance is improved in various ways of improving the plant nutriture, changing the rhizosphere physical and chemical properties, changing the root morphology, adjusting the expression of some salt-resistant genes and the synthesis of related proteins and the like, and the arbuscular mycorrhizal fungi and the organic fertilizer are applied simultaneously, so that saline-alkali stress can be relieved synergistically.

The invention relates to a method for promoting growth of sweet sorghum in saline-alkali soil through arbuscular mycorrhizal fungi. The method includes the steps of preparing and applying an arbuscular mycorrhizal fungi agent. When the arbuscular mycorrhizal fungi agent is prepared, a mixture of farmland soil and organic fertilizer serves as a substrate, and the organic fertilizer and decomposed products of the organic fertilizer can be used as a fungi protecting agent to have a buffering effect on saline ions in soil around the arbuscular mycorrhizal fungi in the early period when the fungi agent is applied to the saline-alkali soil so as to help the arbuscular mycorrhizal fungi to resist saline-alkali stress; the symbiotic relationship is smoothly formed between the arbuscular mycorrhizal fungi and the sweet sorghum, the mineral nutrition of the sweet sorghum is remarkably improved, the sweet sorghum can be helped to resist saline-alkali stress, and the growth of the sweet sorghum is promoted.

The invention discloses a preparation method for algae endophytic fungi exocellular polysaccharide, and relates to a biological method for synthesizing polysaccharide. According to the preparation method, purified polysaccharide is separated from blue algae endophytic fungi DT06 serving as production strains through shaking table or liquid-state submerged cultivation fermentation, wherein the blue algae endophytic fungi DT06 are used as the production strains; the strains are collected at December 20, 2010 with a collection number of CGMCC 4460; and the preparation method specifically comprises the following steps of: 1, performing liquid fermentation by using the blue algae endophytic fungi DT06; 2, performing exocellular polysaccharide separation; and 3, purifying the polysaccharide to finally prepare the pure fungi exocellular polysaccharide product which is shown as the following structural formula. The endophytic fungi DT06 used in the method are separated from the blue algae; and the defects of narrow raw material source, low probability of separation and high production cost existing in the prior art are overcome.

The invention relates to the field of Rhodeus sinensis propagation, in particular to an artificial propagation technology. The invention mainly relates to an artificial propagation technology for Rhodeus sinensis. The artificial propagation technology for the Rhodeus sinensis comprises infrastructure equipment, parent fish selection, parent fish temporary rearing, parent fish maturing acceleration, parent fish spawning, ovum pickup and incubation, wherein the parent fish maturing acceleration, the parent fish spawning, the ovum pickup and the incubation are the core of the artificial propagation technology for the Rhodeus sinensis. According to the artificial propagation technology for the Rhodeus sinensis provided by the invention, a square pond of 1 mu or a cement pit of 10-50m<2> is selected, and is equipped with micropores to enhance oxygen. Parent fishes are characterized in that lateral lines are incomplete, and the back upper place of each spiracle of each parent fish has shoulder patches. Male fishes are red in eye socket, and bright in body color; the abdomen of each female fish has a strip ovipositor. 4.5g / l of VC is added to temporary rearing water; before put, the parent fishes are soaked in 5mmg / l of potassium permanganate for 10 minutes; the density of the potassium permanganate is lower than 0.5kg / m<3>, and a toad of 50g is cultivated in every 4m<2> of water to prevent fungal diseases. 10g / kg of minced earthworm meat and spirulina powder is fed. The female-male ratio of the parent fishes is 1:1, oxytocic is injected after the parent fishes are narcotized by 30mg / lMS-222, the ovum pickup is performed by using Sinanodonta woodiana or unio douglasiae which is 8-10cm wide, and the ratio of the Sinanodonta woodiana or the unio douglasiae to the fishes is 2:3. The Sinanodonta woodiana or the unio douglasiae is harvested once every two days, the ovum-contained Sinanodonta woodiana or the ovum-contained unio douglasiae is moved to a hatching pond, and corresponding ovum-free Sinanodonta woodiana or corresponding ovum-free unio douglasiae is supplemented.

The invention relates to a culture medium for culturing white rot fungi secretion laccase, which relates to a culture medium which cultures fungi secretion function enzyme. The invention solves the problems that white rot fungi laccase which is cultured by the existing culture medium has low laccase yield, which can not meet the requirements of producing laccase in large amount. The culture medium for culturing white rot fungi secretion laccase of the invention comprises 35-45mL corn meal leaching solution, 0.43-0.45g ammonium tartrate, 0.19-0.21g KH2PO4, 0.05-0.056g MgSO4.7H2O, 10.0-10.2mg CaCL2, 0.8-1.2mL inorganic solution, 0.4-0.6mL vitamin solution, and 0.2mL buffer solution of HAc-NaAc whose PH is 7 in each 1000mL fluid medium. The enzyme activity of laccase which is secreted by white rot fungi which is cultured by the invention is 5 times more than the existing culture mediums.

The subject invention relates to novel systems, materials and methods for aseptic production of fungi on a large scale. In particular, the subject invention provides systems, materials and methods for producing endomycorrhizal fungal propagules, including spores and hyphal mycelium, using a two-stratum system supplemented with plant hormones and other natural growth stimulators.

The invention provides a method for promoting accumulation of triterpene in betula platyphylla suk. Suspension cell by utilizing endophytic fungi elicitor. The method utilizes endophytic fungi separated from betula latyphylla suk. bark, the endophytic fungi is prepared into fungi elicitor, appropriate concentration of fungi elicitor is added in appropriate stage of betula platyphylla suk. suspension cell growth, after induction is carried out for a period of time, cells are collected, and endophytic fungi capable of effectively improving accumulation of triterpene is screened out as phomopsis sp. On the basis, induction effect of polysaccharide elicitor and protein elicitor prepared by the fungi is further optimized; 40Mug / ml of polysaccharide elicitor is added on the eighth day of betula platyphylla suk. cell culture, triterpene content in cell after induction for one day reaches up to 29.47mg / g, being 1.78 times that of the control group; 80Mug / ml of fungus protein elicitor is added on the eighth day of betula platyphylla suk. cell culture, induction culture is carried out for four days, and triterpene content in cell reaches up to 34.49mg / g, being 2.47 times that of the control group. In the invention, the type and concentration of the added elicitor, growth stage of betula platyphylla suk. suspension cell added with appropriate elicitor and induction time of elicitor are determined, induction technology of effectively improving accumulation of triterpene in betula platyphylla suk. suspension cell is formed, and important theory and technical basis are laid for betula platyphylla suk. triterpene production by utilizing cell engineering technology, thus the invention has wide application potential and promotion prospect.

The invention provides white fungus and bird's nest ferment fluid having the effect of resisting oxidation and a preparation method of the white fungus and bird's nest ferment fluid. The white fungus and bird's nest ferment fluid having the effect of resisting oxidation comprises the following components in parts by weight: 30-50 parts of white funguses, 20-30 parts of bird's nests and 30-50 parts of opuntia ficus-indica fruits. According to the white fungus and bird's nest ferment fluid and the preparation method thereof disclosed by the invention, the white funguses rich in fungus polysaccharide, the bird's nests rich in animal glycoprotein, and the opuntia ficus-indica fruits rich in vegetable protein are selected as fermenting raw materials, so that the prepared white fungus and bird's nest ferment fluid has good effect of resisting oxidation. Besides, the ferment fluid prepared through collocation and fermentation of the raw materials is comprehensive in nutrition and rich and unique in taste.

The invention discloses a method for planting fungi hexagona apiaria (CCTCC NO.M208121) sporocarp for a rare drug. The method comprises the following steps: the cultivation of a separated and purified hexagona apiaria seed, the cultivation of hypha in a fungus sack, the cultivation of the hexagona apiaria sporocarp and the harvest of the hexagona apiaria sporocarp. The planting of the hexagona apiaria sporocarp through a sack material needs about 2 to 3 months from the production of a strain, inoculation, fungus cultivation, fruiting cultivation to the harvest of the sporocarp; the method has short production period and rapid effect, realizes large-scale cultivation of the hexagona apiaria sporocarp, fundamentally solves the problem of insufficient wild resources, provides abundant drug resources for further research of the active components, the pharmacological action, a pharmaceutical mechanism and a toxicity test of the hexagona apiaria sporocarp, and lays a foundation for better developing and utilizing the hexagona apiaria.

The invention discloses a dendrobium officinale kimura et migo endophytic fungi strain NT66G01 and application thereof. The strain NT66G01 belongs to claviceps in taxonomy, is named Claviceps sp., and is preserved in China Center for Type Culture Collection on July 9, 2014, and the preservation number is CCTCC NO.M2014334. Genome 18s rDNA base sequence of the strain is shown in SEQ ID No.1. Experiment shows that fungus elicitor culture medium and biological bacterial fertilizer prepared by the dendrobium officinale kimura et migo endophytic fungi strain NT66G01 has obvious promotion function to growth of dendrobium officinale kimura et migo, and the dendrobium officinale kimura et migo endophytic fungi strain NT66G01can be used for artificial cultivation of the dendrobium officinale kimura et migo to improve yield of the dendrobium officinale kimura et migo and has obvious application value.

The invention relates to a method for genetic transformation of germinating conidiophore of Curvularia lunata Boed. The method comprises the following steps: culturing the germinating conidiophore of the Curvularia lunata Boed as a receptor by a slide suspension method; co-culturing agrobacterium containing an epiphyte conversion vector system and the germinating conidiophore; and screening transformants with resistibility on an antibiotic culture medium. The system uses the Curvularia lunata Boed as initial bacteria, spore is scratched by 0.9 percent of physiological saline after the Curvularia lunata Boed is cultured on the PDA culture medium for 5 to 7 days, and is used for infection of the agrobacterium after the spore is germinated by the slide suspension culture method. The infected germinating conidiophore obtains resistant transformant by co-culture, bacteriostasis culture and anti-selective agent screening culture of continuous two generations, so that a Curvularia lunata Boed mutant base is constructed to provide rich screening strain sources for weak pathogenicity and pathogenic defective Curvularia strain, and meanwhile can be used for cloning related gene of Curvularia lunata Boed pathogenicity so as to lay a foundation for further investigating the pathogenesis mechanism of the Curvularia lunata Boed strain.

The invention discloses a method for promoting side slope green retrieval through mycorrhiza inoculation. Firstly, stolons of plants undergo cuttage in a matrix mixed with arbuscular mycorrhizal fungi, and watering and maintenance are performed for propagation expanding; the plants obtained through the propagation expanding are taken out, the stolons are sheared into stem sections with adventitious roots and the matrix; fermentation broth of ectotrophic mycorrhiza, side slope plant seeds and an organic fertilizer for sterilization are taken and mixed to obtain a mixture; a base material is spray-seeded on a side slope; the obtained plant stem sections and the mixture are mixed and spray-seeded; arbor seedlings are transplanted, the ectotrophic mycorrhiza and / or the arbuscular mycorrhizal fungi are taken to wrap broken roots of the arbor seedlings or the ectotrophic mycorrhiza and / or the arbuscular mycorrhizal fungi is scattered to pits, the arbor seedlings are planted in the pits; the stem sections spray-seeded with a bacterium carrying agent can be used for inoculating the arbuscular mycorrhizal fungi to all the side slope plants, the side slope plants and the spray-seeded stolon section plants grow rapidly, the demand for rapid green retrieval at the earlier stage of side slope green retrieval can be met, mycorrhiza fungi inoculation is specifically conducted on the arbor seedlings, and the demand for keeping vegetation for a long period of the side slope can be met.

The invention discloses a sclerotium oyster-cap fungus donghua tiger milk number 1bacterial strain and it application. The bacterial strain belongs to basidiomycetes oyster-cap fungus section, alternate name is tiger-milk bacterium, nuclear ear mushroom,poria cocos wolf oyster-cap fungus, nanyang oyster-cap fungus and it is preserved on September 25th, 2005 year whose entreasure identification serial number is CGMCC No.1545. It has strong anti- polygamy, bacterial filament upgrowth temperature is 18 to 38 DEG C and the most suitable temperature is 24 to 27 DEG C, bacterial filament upgrowth ph is 5.5 to 6.8 and the most suitable Ph is 5.8 to 6.5. The bacterial strain is a typical high temperature variety which can grow in simple shade in hot summer. The fresh mushroom has delicious flavor, good mouthfeel and keeping quality identity and the dried mushroom has good color and fragrance. The sclerotium oyster-cap fungus donghua tiger milk number 1 has abundant nutrition ingredient and is not only edible but also a treasure dual purpose large scale fungi both for food and medicine. Also the fungi is an excellent health care food and has the function of prolong life and health care beauty treatment for elder on condition of long-term consumption.

The invention discloses a rice medium formula and culture method of Bursaphelenchus xylophilus endoparasitic fungus Esteya vermicola, and belongs to the technical field of microorganisms. Compared with a conventional PDA solid culture method and a PDB liquid culture method, a rice medium preparation and culture method of the Bursaphelenchus xylophilus endoparasitic fungus Esteya vermicola not onlyshortens the culture time of the bacteria, but also obtains a high-purity, high-density fungal spore suspension. The method is applied to the control of Bursaphelenchus xylophilus, and has significant economic, ecological and social benefits.

Therapeutic compositions made from the herb Tribulus terrestris and methods of making and using the same are provided. The therapeutic compositions include an enriched extract having an increased spirostanol saponin content that is prepared from the harvested Tribulus terrestris L. The enriched extract is prepared using discrete hydrolysis, separation and enrichment steps. The resulting therapeutic may be combined with a cream base and is useful for treating bacterial, fungal, and viral infections, particularly gynecologic infections. This product was also found to be very successful in suppository form for the treatment of vulvo-vaginal, vulvo-hemorrhoidal and colonic conditions.

Nucleotide or amino acid sequences that may be used in the detection and / or identification for an ochratoxigenic fungus or in the construction of an atoxigenic strain of an ochratoxigenis fungus. The fungus may be of the genus Aspergillus, species carbonarius, niger, alliaceus, or foetidux. The fungus may also be of the genus Penicillium, species verrucosum.

The invention belongs to the technical field of organisms and discloses a method for removing explant endophyte during plant tissue culturing. The cuttage soil can be disinfected by 50% of carbendazim, cuttage twigs are disinfected by sterile water solution containing 200 mg / L of amphotericin and 100 mg / L of streptomycin sulfate, and new semi-lignified twigs can be treated in advance by sterile water solution containing 100 mg / L of amphotericin and 50 mg / L of streptomycin sulfate. Accordingly, bacterial and fungi on the surface of and inside the explant such as aspen can be effectively removed, and sufficient vigor of the explant can be kept.

The invention discloses a penicillium rubens-yielding strain of decomposed fungus of wheat straws in cold region and a fermental cultivation method and an application thereof and belongs to the fieldof separation, preparation and application of decomposed fungus of wheat straws in cold region. The invention firstly discloses a separated penicillium rubens-F1 strain, the microbial preservation number of which is CGMCC No. 15100. The separated penicillium rubens-F1 strain can grow at different temperatures and still realizes vigorous growth in a low temperature condition. The invention furtheroptimizes related parameters of the fermental cultivation method of the separated fungi strain. The separated fungi strain can ferment the strains in the low temperature condition and can be independently applied to or jointly applied to fermenting the straws with an EM inoculant; by fermenting the wheat straws for 25 days at the low temperature after the fermenting liquor of the strain is mixed with the EM bacterial solution, the straw fermenting effect is improved obviously, and the fermenting rate is increased by 43.9%.

The invention discloses a new species Xylariaceae sp. DPZ-SY43 of mangrove rhizosphere cellulose decomposed fungi and application thereof. Xylariaceae sp. DPZ-SY43 is preserved in the China General Microbiological Culture Collection Center (CGMCC) with a preservation number of CGMCC No:4871 on May 16, 2011 at Institute of Microbiology, Chinese Academy of Sciences, No.3, No.1 West Beichen Road, Chaoyang District, Beijing, China. The fungus has the activity of producing cellulase, can be used for producing cellulase, and thus has important value in production and utilization of cellulase. Furthermore, the Xylariaceae sp. DPZ-SY43 can be used for preparing biological organic matter degrading bacterial manure.

The invention discloses immature orange fruit endophytic fungi for generating hesperidinase. The bacterial strain is Aspergillus tamarii( Aspergillus tamarii ) ZS002, and is preserved in the China General Microbiological Culture Collection Center(CGMCC) on June 28, 2019, with the preservation No. of CGMCC NO.18105. In the scheme, enzymes generated by microorganisms are used as a catalyst to catalyze transformation of the hesperidin into hesperetin, the reaction efficiency is high, the selectivity is high, few by-products exist, the steps are simple, and the immature orange fruit endophytic fungi is green, environmental-friendly and pollution-free.

The invention relates to a DNA sequence of a fungus variety as well as a molecular identification method for identifying a variety of the fungus by using the DNA sequence. An ITS1-5.8S-ITS2 sequence of the dictyophora indusiata is as shown by SEQ NO.1; an ITS1-5.8S-ITS2 sequence of dictyophora duplicate is as shown by SEQ NO.2; and an ITS1-5.8S-ITS2 sequence of the dictyophora phalloidea is as shown by SEQ NO.3. When in identification, a genome of a to-be-identified sample is firstly extracted, an ITS1-5.8S-ITS2 fragment is amplified in a PCR manner, then cloning and sequencing are performed,the obtained sequence is compared separately with the sequences shown by SEQ NO.1, SEQ NO.2 and SEQ NO. 3 so as to determine the variety of the dictyophora indusiata, dictyophora duplicate or dictyophora phalloidea, the variety of the dictyophora indusiata, dictyophora duplicate and dictyophora phalloidea can be accurately distinguished and determined, so that a reliable technology can be providedfor identifying the species, protecting the varieties, controlling the quality of the strain, effectively breeding the strain and artificially cultivating the dictyophora.

The invention discloses a method for promoting growth of orchidaceae through Ceratobasidium sp.. A symbiotic relationship is established between a strain of ceratobasidium and protocorm / rhizomes and seedlings of the orchidaceae to promote growth of the orchidaceae, wherein the strain of ceratobasidium is Ceratobasidium sp. of ceratobasidiaceae, has the Latin name of Ceratobasidium sp., the GenBankregistration number of MG669582 and the biological preservation number of CGMCC No.15373. Through the promoting function of the Ceratobasidium sp. on growth of the orchidaceae, a foundation is laid for application of the Ceratobasidium sp. in efficient breeding of orchidaceae and resource protection of wild orchidaceae.

The present invention discloses a fusarium oxysporum strain and belongs to the technical field of pathogenic microorganism isolation. The fusarium oxysporum is fusium oxysporum Hs-f (Fusarium sp.), isdeposited on August 29, 2019 in China General Microbiological Culture Collection Center and has a deposit number of CGMCC NO:18130, the address is No.3, No.1 courtyard, Beichen West Road, Chaoyang District, Beijing, and a unit asking for deposition is Peanut Institute of Shandong province. The peanut rot pathogen fungus is isolated and purified from peanut rot disease seed kernels. Inoculation experiments prove that the fusarium oxysporum strain has high pathogenicity, is also free of tissue specificity, and has pathogenicity to peanut roots, stems, leaves and seed kernels.

The invention relates to a method for field culture of cordyceps sinensis and vegetation recovery in alpine-cold cordyceps growing areas. Cordyceps sinensis is an insect herb which is formed by hepialus larvae (host) after being infected by fungus cordyceps sinensis and is a special product of a biological chain of fungus, insects and plant in a special habitat. The invention belongs to Field Culture of Wild Medicine biological engineering and has the advantages of proliferating fungus and the hosts in alpine-cold road and field growing areas, increasing and protecting alpine meadow and alpineshrub and meadow vegetation, compensating the activity of the biological chain, developing geographical (biological) resources of cordyceps sinensis habitat which is difficult to simulate by human, decreasing natural enemy hazard to protect the rare species of cordyceps sinensis with stable production and good quality, and protecting and improving the fragile alpine meadow vegetation. The methodadopts different species of hosts for egg-increasing assistance by levels and regions to increase the hatching rate and the larva density; the fungus is separated and purified, and the fungus source is provided through ferment, so that the infection rate of insects by fungus is improved; meanwhile, the favorite plant of host larva is compensated, and growth regulator and special fertilizer are applied to protect the alpine meadow vegetation.