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Preparation method for algae endophytic fungi exocellular polysaccharide

A technology of endophytic fungi and exopolysaccharides, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of limited raw material sources, difficult separation, high production costs, etc.

Inactive Publication Date: 2012-07-11
HEBEI UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The technical problem to be solved by the present invention is: to provide a preparation method of exopolysaccharide of algal endophytic fungus, the endophytic fungus DT06 used is isolated from cyanobacteria, which overcomes the lack of wide sources of raw materials in the prior art and is not easy to separate and the disadvantages of high production costs

Method used

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  • Preparation method for algae endophytic fungi exocellular polysaccharide
  • Preparation method for algae endophytic fungi exocellular polysaccharide
  • Preparation method for algae endophytic fungi exocellular polysaccharide

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Experimental program
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Effect test

Embodiment 1

[0055] The producing bacterium is cyanobacterium endophytic fungus DT06. This strain has been patent preserved in China General Microorganism Culture Collection Center. The preservation date is: December 20, 2010, and the preservation number is: CGMCC4460. The nucleotide sequence of this strain is shown in the sequence list . The name of the strain is Simplicillium lanosoniveum (J.F.H.Beyma) Zare & W.Gams var.tiamjinienss Q.L.Dong.

[0056] Slant medium formula: glucose 0.5g, yeast extract 0.05g, peptone 0.1g, agar 1g and water 100ml, make a test tube slant, pick mycelium and insert it into the slant.

[0057] The first step, cyanobacteria endophyte TD06 liquid fermentation

[0058] The formula of the fermentation medium is: sucrose 30g / L, sodium nitrate 2g / L, potassium dihydrogen phosphate 0.5g / L, potassium chloride 0.1g / L, magnesium sulfate heptahydrate 0.1g / L, iron sulfate 0.01g / L;

[0059] The cyanobacteria endophyte DT06 is subjected to liquid fermentation, and the sha...

Embodiment 2

[0068] The producing bacterium is cyanobacterium endophytic fungus DT06. This strain has been patent preserved in China General Microorganism Culture Collection Center. The preservation date is: December 20, 2010, and the preservation number is: CGMCC4460. The nucleotide sequence of this strain is shown in the sequence list . The name of the strain is Simplicillium lanosoniveum (J.F.H.Beyma) Zare & W.Gams var.tianjinienss Q.L.Dong.

[0069] Slant medium formula: glucose 0.75g, yeast extract 0.075g, peptone 0.15g, agar 1.25g and water 100ml, make a test tube slant, pick mycelium into the slant.

[0070] The first step, cyanobacteria endophyte TD06 liquid fermentation

[0071] The formula of the fermentation medium is: sucrose 45g / L, sodium nitrate 2.5g / L, potassium dihydrogen phosphate 0.75g / L, potassium chloride 0.5g / L, magnesium sulfate heptahydrate 0.3g / L, iron sulfate 0.05g / L ;

[0072] The cyanobacteria endophyte DT06 is subjected to liquid fermentation, and the shaker ...

Embodiment 3

[0081] The producing bacterium is cyanobacterium endophytic fungus DT06. This strain has been patent preserved in China General Microorganism Culture Collection Center. The preservation date is: December 20, 2010, and the preservation number is: CGMCC4460. The nucleotide sequence of this strain is shown in the sequence list . The name of the strain is Simplicillium lanosoniveum (J.F.H.Beyma) Zare & W.Gams var.tianjinienss Q.L.Dong.

[0082] Slant medium formula: glucose 1g, yeast extract 0.1g, peptone 0.2g, agar 1.5g and water 100ml, make a test tube slant, pick mycelium and insert it into the slant.

[0083] The first step, cyanobacteria endophyte TD06 liquid fermentation

[0084] The formula of the fermentation medium is: sucrose 60g / L, sodium nitrate 3g / L, potassium dihydrogen phosphate 1g / L, potassium chloride 1g / L, magnesium sulfate heptahydrate 0.5g / L, iron sulfate 0.1g / L;

[0085] The cyanobacteria endophyte DT06 is subjected to liquid fermentation, and the shaker fer...

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Abstract

The invention discloses a preparation method for algae endophytic fungi exocellular polysaccharide, and relates to a biological method for synthesizing polysaccharide. According to the preparation method, purified polysaccharide is separated from blue algae endophytic fungi DT06 serving as production strains through shaking table or liquid-state submerged cultivation fermentation, wherein the blue algae endophytic fungi DT06 are used as the production strains; the strains are collected at December 20, 2010 with a collection number of CGMCC 4460; and the preparation method specifically comprises the following steps of: 1, performing liquid fermentation by using the blue algae endophytic fungi DT06; 2, performing exocellular polysaccharide separation; and 3, purifying the polysaccharide to finally prepare the pure fungi exocellular polysaccharide product which is shown as the following structural formula. The endophytic fungi DT06 used in the method are separated from the blue algae; and the defects of narrow raw material source, low probability of separation and high production cost existing in the prior art are overcome.

Description

technical field [0001] The technical scheme of the invention relates to the synthesis of polysaccharides by using biological methods, in particular to a method for preparing exopolysaccharides of algal endophytic fungi. Background technique [0002] Plant endophytic fungi can generally synthesize a variety of special natural extracellular products, and the structures and properties of extracellular products produced by different endophytic fungi are also different. [0003] CN1594587 discloses the preparation and application of a fungal extracellular polysaccharide complex, which is to extract the extracellular polysaccharide complex from the culture solution of a Cordyceps fungus-Corvicomyces chinensis. The source of raw materials for this invention is not extensive, and the production cost is high ; CN1974753 discloses a fermentation and separation method that can obtain a large amount of fungal exopolysaccharides. The disadvantage of the inventive method is that the produ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/04C12R1/645
Inventor 董庆霖陈博邢向英
Owner HEBEI UNIV OF TECH
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