35 results about "Rare species" patented technology

The invention relates to the field of genetic resource conservation, particularly relates to the field of animal paternity identification, and discloses a method of establishing an animal paternity identification system on basis of whole genome STR. The method comprises the following steps: 1) looking up whole genome STR loci; 2) preliminarily screening the STR loci; 3) carrying out a primer design; 4) randomly sampling animal populations and extracting genome DNA; 5) verifying the population diversity of the STR loci; 6) carrying out Hardy-Weinberg equilibrium inspection on the STR loci with genetic polymorphism, and screening out the STR loci with the genotype frequency distribution which meets the Hardy-Weinberg equilibrium; 7) evaluating the forensic medicine application value of the STR loci; 8) establishing the STP animal paternity identification system. The method is convenient to operate; the constructed paternity identification system has a paternity exclusion probability of more than 0.99, and is capable of accurately carrying out animal paternity identification, therefore, the paternity identification system can be used for guiding the breeding of rare species, and the identification and breeding of economic species.

The invention relates to a method for constructing a sinocyclocheilus rhinocerous heart cell line. The method comprises the following steps: 1, obtaining of sinocyclocheilus rhinocerous heart tissues, wherein sinocyclocheilus rhinocerous is sterilized through combination of penicillin and ethyl alcohol; 2, primary culture, wherein a culture solution M199 which contains glutamine, penicillin, streptomycin, streptomycin sulphate and amphotericin is adopted for culture, and the osmotic pressure of the culture solution is 280-330 mOsm / l; 3, secondary culture, wherein when the tenth generation is reached, cell culture fluid is replaced with base culture fluid, and the sinocyclocheilus rhinocerous heart cell line is constructed successfully. According to the method for constructing the sinocyclocheilus rhinocerous heart cell line, the obtained sinocyclocheilus rhinocerous heart cell line is in the fibrous form and can conduct continuous passage and be directly applied to research of biological characteristics, requirements of genetic resource preservation and theoretical research conducted on the rare species, namely the sinocyclocheilus rhinocerous, can be satisfied, meanwhile, the heart cell line is the first heart cell line of cave fishes in China, and the foundation is laid for cave adaptability research at the cellular level.

The invention discloses a kit used for a paternity test of giant pandas. 68 allelic genes of 11 STR gene loci, obtained by microsatellite study, of the giant pandas are taken as typing standard substances, and parameters of annealing temperature and magnesium ion concentration in a PCR process of the STR gene loci are optimized, wherein the typing standard substances correspond to the allelic genes of the gene loci one by one, so that the microsatellite typing can be performed accurately. The kit of the invention can perform the paternity test and genetic monitoring accurately on species groups of captive giant pandas, complete genealogy, reorganize blood relationship and provide very useful molecular markers for the study in the aspects such as the molecular evolution of the giant pandas, the population migration and the behavioral ecology of wild giant pandas and the like in future, and has very significant theoretical and practical significance on the formulation and implementationof the conservation and breeding plans of rare species.

The invention relates to a method for field culture of cordyceps sinensis and vegetation recovery in alpine-cold cordyceps growing areas. Cordyceps sinensis is an insect herb which is formed by hepialus larvae (host) after being infected by fungus cordyceps sinensis and is a special product of a biological chain of fungus, insects and plant in a special habitat. The invention belongs to Field Culture of Wild Medicine biological engineering and has the advantages of proliferating fungus and the hosts in alpine-cold road and field growing areas, increasing and protecting alpine meadow and alpineshrub and meadow vegetation, compensating the activity of the biological chain, developing geographical (biological) resources of cordyceps sinensis habitat which is difficult to simulate by human, decreasing natural enemy hazard to protect the rare species of cordyceps sinensis with stable production and good quality, and protecting and improving the fragile alpine meadow vegetation. The methodadopts different species of hosts for egg-increasing assistance by levels and regions to increase the hatching rate and the larva density; the fungus is separated and purified, and the fungus source is provided through ferment, so that the infection rate of insects by fungus is improved; meanwhile, the favorite plant of host larva is compensated, and growth regulator and special fertilizer are applied to protect the alpine meadow vegetation.

The invention relates to a propagation method of fruit trees, and discloses a propagation method of wufu persimmon seedlings. The method comprises the steps of cleaning and disinfecting wufu persimmonpulp and then preparing the wufu persimmon pulp into fruit leaven; putting wufu persimmon seeds into the fruit leaven to be soaked, and promoting seed germination and rooting; after transplanting, proportioning quantitative clean water for the fruit leaven, watering bud seedlings periodically, using the fruit leaven to provide original nutrients for seedlings, and finally enabling the seedlings to grow into normal and robust plants. In this way, the endangered rare species is rescued. By means of the propagation method of the wufu persimmon seedlings, the survival rate of the wufu persimmon seed-raising seedlings exceeds 80%, the limit of non-propagation of the wufu persimmon seedlings is broken through, and the propagation method is significant in protecting, studying, protecting, applying and popularizing the species.

The invention relates to a construction method of a kidney cell line of Schizothorax taliensis. The method comprises following steps: 1), obtaining of kidney tissue of the Schizothorax taliensis: potassium permanganate and alcohol are combined to disinfect the Schizothorax taliensis; 2), primary culture: an L-15 or DMEM / F12 culture solution containing cell growth factors, penicillin, streptomycin and amphotericin B is adopted for culture, and osmotic pressure of the culture solution is 280-300 mOsm / L; 3), subculture: when subculture is performed to the tenth generation, the cell culture fluid is replaced with a basic culture solution, and the kidney cell line of the Schizothorax taliensis is constructed successfully. With the adoption of the construction method, the form of the obtained kidney cell line of the Schizothorax taliensis is fibroid, the kidney cell line can realize continuous passage, can be directly applied to research of biological characteristics, can meet the requirements for germplasm resource preservation and theoretical research of the Schizothorax taliensis as a rare species and is the first cell line of the Schizothorax taliensis, thereby laying the foundation for research of plateau adaptability of the cell level of the Schizothorax taliensis.

The invention relates to an oil removing device, in particular to a horizontal-type oil removing device for rare earth feed liquid. The invention aims to provide the horizontal-type oil removing device for the rare earth feed liquid. In order to achieve the technical aim, the horizontal-type oil removing device for the rare earth feed liquid comprises a filter cartridge, a feeding device, an oil absorption device, an L-shaped connecting plate, a piston, a long-stroke air cylinder I and the like. The oil absorption device is arranged at the right side of the feeding device. The L-shaped connecting plate is arranged at the left side of the feeding device. The lower end of the L-shaped connecting plate is connected with the top of the filter cartridge in a welding mode. The piston is arranged inside the filter cartridge and is further matched with the filter cartridge, and the piston and the filter cartridge are arranged to be movably connected. The horizontal-type oil removing device for the rare earth feed liquid is of a horizontal-type structure, no high and large space can be occupied, there is no need for enterprises to set up tall workshops, and the occupation of business capital is reduced.