33 results about "Aberrant spleen" patented technology

The invention discloses a colorectal cancer early hepatic metastasis animal model and a construction method and application thereof. Cell suspension concentration of the colorectal cancer early hepatic metastases animal model is 2.5*107 / ml, about 0.1ml of colorectal cancer cells are injected intrasplenically for 3min; a Vetbond 3M tissue adhesive is adopted for sealing an incision, and then the spleen is put back. The colorectal cancer early hepatic metastases animal model in different metastatic characteristics is constructed innovatively; differences of in-vitro nuclein uptake ratios of cancer cells in different metastatic potentials are verified; statistic differences of biological characteristic indexes of different cancer strains and metastatic nodules are found; early hepatic metastatic tumor development biological characteristics are reflected completely, and a novel method is provided for discovery and diagnosis of early hepatic metastasis.

The invention discloses traditional Chinese medicine composition for treating the Alzheimer's disease as well as a preparation method and an application of the traditional Chinese medicine composition. The traditional Chinese medicine composition comprises milkvetch roots, Chinese angelica, red peony roots and prince's-feather fruits. Research indicates that the traditional Chinese medicine composition can improve the spatial learning ability and the spatial exploration ability of Kunming mice suffering from the Alzheimer's disease, increase visceral indexes of brains and spleens of the mice suffering from the Alzheimer's disease, reduce the permeability of Alzheimer's disease blood brain barriers, decrease Alzheimer's disease brain tissue hippocampal Abeta, IL-6 and IL-12 protein expression levels and have no obvious effect on weight of Alzheimer's disease models, namely, the Kunming mice. The results indicate that the traditional Chinese medicine composition is good in treatment effect on the Alzheimer's disease, so that the traditional Chinese medicine composition has the broad application prospect in the field of treatment of the Alzheimer's disease.

The invention discloses an application of Yigong powder in preparation of drug for treating splenomegaly and mitochondrial damage caused by anemia of chronic disease, infection or inflammation disease. The Yigong powder is applied to prepare a drug for treating splenomegaly and mitochondrial damage caused by anemia of chronic disease, infection or inflammation disease, the adaptation disease of the formula is enlarged, and a new treatment drug is provided for these patients with uncontrolled basic disease and anemia of chronic disease. The Yigong powder can improve the effect of hemoglobin ofanemia of chronic disease (ACD), and further correct the anemia of chronic disease; meanwhile, the Yigong powder can obviously reduce the splenomegaly caused by inflammation; through reducing spleen phagocytosis to red cells, the ACD anemia can be improved; besides, the Yigong powder also can protect mitochondria and lighten the mitochondria damage caused by inflammation; through the autophagy wayof mitochondria, the injured autophagy is removed, and the influence of inflammation on an organism is reduced.

The invention provides a method for constructing a spleen tissue cell line of acipenser dabryanus. The method mainly comprises the following steps: (1), an MEM (minimum essential medium) culture solution containing fetal calf serum, human epidermal growth factors, human fibroblast-like cell growth factors and acipenser dabryanus serum and having the pH of 7.2-7.4 is prepared and stored in a refrigerator at the temperature of 4 DEG C for standby application; (2), spleen tissue is separated from the acipenser dabryanus body and subjected to primary culture with a tissue block culture method; (3), when the cell growth forms a single layer and the bottom coverage rate is higher than 70%, 0.25% trypsin digestion is adopted for subculture; (4), vigorously growing spleen cells with uniform forms are subjected to liquid nitrogen cryopreservation and resuscitaion culture. The spleen tissue cell line constructed with the method is fast to reproduce, has multiple forms, is fibroblast-like, and can realize continuous passage and cryopreservation resuscitation. The construction method is simple in technology, easy to operate, can be popularized to culture of spleen cells of other sturgeons and can be expected to be used for acipenser dabryanus species resource conservation as well as separation and identification of sturgeon diseases especially virus pathogens.

The invention provides application of a PD1 molecule inhibitor in T lymphocytic leukemia treatment. Concretely, the invention provides application of the PD1 molecule inhibitor, and the PD1 molecule inhibitor is used for preparing a medicine composition for inhibiting T-ALL cell differentiation and proliferation, and / or treating acute T lymphoblastic leukemia. The invention indicates that anti-PD-1 antibody therapy inhibits proliferation of T-ALL cells in spleens, and provides a certain experimental basis for the anti-PD-1 antibody clinically treating T-ALL.

The invention relates to an in-vitro preparation method of transfer factors capable of resisting duck viral hepatitis. In the method, the transfer factors capable of resisting the duck viral hepatitis are prepared through the induction of duck hepatitis virus by utilizing an in-vitro lymphocyte cell culture method; and chicken spleen or peripheral blood is used as a basic raw material to prepare single-layer lymphocyte cells, and then the single-layer lymphocyte cells are induced and cultured through phytohemagglutinin and the duck hepatitis virus, so that a large number of specific transfer factors capable of resisting the duck hepatitis virus are generated in vitro.. The transfer factors can effectively inhibit the virus of duck viral hepatitis, so that the normal bodies cannot be infected with the duck viral hepatitis, and the radical pre-prevention action can be realized. The method provided by the invention has the characteristics of being simple and easy to operate and the like.

The invention discloses an application of LukS-PV protein in preparation of a medicine for inducing leukemia cell differentiation. The leukemia cell refers to a human promyelocyte leukemia cell HL-60 or human monocyte leukemia cell THP-1. The LukS-PV protein can be massively expressed by using a genetic engineering technology, can be specifically combined with a target cell but not cause the dissolution of the target cell, is small in molecular weight, is easily modified genetically and chemically; and through gene modification, the induction activity is expected to be improved and the toxic and side effects are expected to be reduced; experiment shows that LukS-PV can induce the HL-60 cell to differentiate to marrow monocyte in vitro and induce the THP-1 cell to differentiate to macrophage; LukS-PV can inhibit leukemia cell growth in vivo and reduce infiltration of the leukemia cell to the liver, the spleen and the peripheral blood; therefore, the LukS-PV protein is expected to be developed into a novel leukemia treatment medicine, thereby opening up a novel application field for bacterial toxin.

The invention relates to a preparation method for immortalized lymphocytes. Lymphocytes are obtained by infecting animal spleens with leukemia virus of the same animal; the lymphocytes which are infected by leukemia virus are cultivated; and then the immortalized lymphocytes are obtained.

The invention relates to a Sinocyclocheilus anshuiensis spleen cell line construction method. The construction method comprises the following steps: (1) obtaining a Sinocyclocheilus anshuiensis spleen: directly disinfecting a fish body by adopting ethyl alcohol; (2) performing primary culture: culturing by adopting B-15 culture solution containing glutamine, fetal calf serum, penicillin, streptomycin, gentamicin and amphotericin B; (3) subculturing: when culturing to the eighth generation, changing the cell culture solution into basic culture solution, thus successfully constructing a Sinocyclocheilus anshuiensis spleen cell line. The Sinocyclocheilus anshuiensis spleen cell line obtained by the construction method can realize continuous passage, is directly applied to biological characteristic research, and can meet the requirements of germplasm resource conservation and theoretical research on Sinocyclocheilus anshuiensis as a rare species; more importantly, the cell line is the first cell line of cave fish in China, and lays the foundation for the cellular level cave adaptability research.

The invention relates to application of black-bone chicken blood powder. The black-bone chicken blood powder can be used for regulating nonspecific immunological competence, and is used for promoting production of cytokines, promoting multiplication of immune cells of the spleen and promoting the activity of natural killer cells and macrophages.

The invention relates to an in vitro preparation method of an avian infectious laryngotracheitis virus resisting specific transfer factor. The in vitro production of the avian infectious laryngotracheitis virus resisting specific transfer factor is achieved by preparing a lymphocyte monolayer from chicken spleen or peripheral blood, and conducting induced culture to human lymphocytes by virtue of phytohemagglutinin and avian infectious laryngotracheitis viruses. The specific transfer factor, which is prepared by the preparation method disclosed by the invention, can be used for preventing and treating avian infectious laryngotracheitis, and meanwhile, the specific transfer factor can be used for enhancing chicken immunity. The invention, as a relatively ideal avian infectious laryngotracheitis virus resisting specific transfer factor preparation method, has the advantages of strong specificity, low in cost, high yield, good effect and the like.

The invention provides application of raspberry polysaccharide in the preparation of medicament for preventing or treating toxic and side effect of chemotherapeutics. After using chemotherapeutics, the human body certainly produces toxic side effects, such as liver, kidney and / or spleen injury. The research of the present invention find that raspberry polysaccharide can effectively reduce the side effects of organ damage caused by the chemotherapeutics, and in particular amount can reduce toxic and side effect but not reduce the curative effect of chemotherapeutics, and even enhance the curative effect, so as to provide novel options for clinical medication of cancer patients.

The invention discloses an application of salvia miltiorrhiza polysaccharide in preparation of anti-inflammatory drug or health care products. An applicant observes influence of salvia miltirorrhiza polysaccharide to DNFB (dinitrofluorobenzene) induced mice auricle allergic reaction, influence of salvia miltirorrhiza polysaccharide to in vitro mice spleen lymphopoiesis and influence of salvia miltirorrhiza polysaccharide to expression of a DNFB induced mice contact dermatitis spleen inflammatory factor by virtue of in vitro cells and molecular biological experiments and finds that the salvia miltirorrhiza polysaccharide has anti-inflammatory effect, thus new application of the salvia miltirorrhiza polysaccharide is found.

The invention provides a prescription for treating bladder calculi. The prescription comprises, by weight, 30g of salix babylonica leaf, 30g of phaseolus calcaratus, 30g of stigma maydis, 15g of talcum powder and 15g of cortex phellodendri. The prescription for treating the bladder calculi has the advantages that the prescription is applicable to patients, of various ages, with the bladder calculi, and the prescription is capable of comprehensively regulating the functions of internal organs such as the kidney, spleen and liver by using the concept of communicating the whole body, tonifying the kidney and benefiting qi, restoring the water controlling and qi transformation managing functions of the kidney, further preventing feculence from precipitating to form the calculi, strengthening and activating the spleen, dispelling dampness and promoting diuresis, allowing urine not to be heated to form the calculi, soothing the liver and regulating qi at the same time, allowing qi formation and qi rise and fall to be performed orderly, and allowing water to circulate normally to make calculi formation difficult; after the passages for forming new calculi possibly are blocked, the medicine effects of regulating qi and relieving pain, promoting diuresis and removing blood stasis, eliminating stagnation and dredging blocking, and treating stranguria and discharging the calculi of the prescription are actually brought into play, and the bladder calculi is cured comprehensively.

The present invention discloses a preparation method of a duck natural immune enhancer based on a traditional Chinese medicine extract. The preparation method comprises the following steps: (1) establishment of a rutin standard curve; (2) extraction of astragalus membranaceus flavonoids; and (3) calculation of astragalus membranaceus flavonoid concentration. The provided duck natural immune enhancer based on the traditional Chinese medicine extract can significantly increase levels of duck serum cytokines of IL-1-beta, IFN-alpha and IFN-beta, up-regulates tissue expression levels of natural immune-related genes RIG-I, TLR3, TLR7, IFN-alpha, IFN-beta, IL-1-beta and TNF-alpha, inflammation-related genes COX2 and iNOS, and cell apoptosis-related gene Caspase3 in duck immune organs of liver, spleen, thymus and bursa of fabricius, and enhances duck natural immune response and disease resistance performance.

The invention provides an application of omeprazole in preparing a medicine for treating autoimmune disease. Based upon experiments, the omeprazole, through oral administration, can take a concrete effect on relieving the autoimmune disease, in particular inflammatory injury caused by the autoimmune disease. Further experiments show that the omeprazole can down-regulate mRNA levels of four proinflammatory cytokines, namely [gamma] interferon in a spleen with EAN (experimental allergic neuritis), tumor necrosis factor [alpha], interleukin 6 and interleukin 17, and meanwhile, the omeprazole can up-regulate mRNA levels of two proinflammatory cytokines, namely interleukin 4 and interleukin 10. Moreover, cytokine expression profile of ELISA detection also shows a similar trend in a spleen mononuclear cell culture supernatant. On the basis of the pharmacological properties, the omeprazole, which can take the effect on treating the autoimmune disease, is applicable to preparation of the medicine for treating the autoimmune disease.

The invention relates to application of black-bone chicken blood powder. The black-bone chicken blood powder can be used for regulating nonspecific immunological competence, and is used for promoting production of cytokines, promoting multiplication of immune cells of the spleen and promoting the activity of natural killer cells and macrophages.

The invention provides a method for extracting hyaluronidase from a bovine spleen. The method comprises the following steps: soaking the bovine spleen in normal saline with a certain concentration, adding extracting liquid to extract, triturate and extract, centrifuging and taking supernatant, adding sodium chloride to remove impurities, adding salt to perform salting-out, and performing dialysis to prepare the refined hyaluronidase. The method is used for extracting the hyaluronidase from the bovine spleen, simplifies the steps, is easy to operate, is high in extraction rate, purity and enzyme activity of the hyaluronidase, and explores a new approach for extraction of the hyaluronidase.

The invention relates to traditional Chinese medicine dissolved granules for changing a focus of diabetes through conditioning transportation and transformation of a human spleen. The dissolved granules are prepared from traditional Chinese medicines such as prunella vulgaris, psidium guajava, glycyrrhiza uralensis, lycium barbarum, radix astragali, raw rehmannia glutinosa and radix glycyrrhizae preparata. According to the dissolved granules, by the clinical thinking of treatment according to syndrome differentiation, the treatment according to syndrome differentiation does not regards static and morphological-seen disease as an inspection target and lays stress on the theory that five internal organs are all the weak and are good at eliminating diabetes; through regulating the yin and yang and deficiency and excess of a human body, improving the status of derangement and malnutrition of qi and blood and regulating metabolic balance so as to help recover the functions of islet cells, clear out a diabetes pathogenesis source, i.e., sugar toxin, break through a bioshield around an islet beta cell and an insulin receptor, rapidly eliminate harmful acid peptides on an islet receptor face and activate the insulin receptor in blood, the metabolic disorder of in-vivo glucose, protein and lipids can return to normal condition, so that the regulation on cell secretion is strengthened, the cell parasecretion induced by the long-term usage of blood sugar reducing Western medicines is restored, the human immunity is enhanced, and thus, the aims of treating the disease and improving health are achieved.

The invention belongs to the technical field of medicines and aims at providing an efficient anti-inflammation and pain-easing drug with low side effects and a preparation method of the drug. According to the technical scheme, the anti-inflammation and pain-easing drug is an oral preparation prepared from the following traditional Chinese medicinal materials in parts by weight: 30-40 parts of purpleflower holly leaves, 8-12 parts of fructus forsythia, 60-70 parts of tangerine leaves, 4-6 parts of jasmine, 2 parts of common threewingnut root and 2 parts of lappaconitine. The drug has very remarkable pain-easing effect, is capable of strongly inhibiting acute and chronic inflammation, exudative inflammation and connective tissue hyperplasia inflammation, has no obvious influence to spleens and thymus glands and is low in toxic and side effects.

The invention relates to a preparation method of an avian infectious laryngotracheitis virus resisting specific transfer factor. The avian infectious laryngotracheitis virus resisting specific transfer factor is prepared by the following steps: preparing a lymphocyte monolayer from chicken spleen or peripheral blood; then, inducing and culturing lymphocytes by virtue of phytohemagglutinin and avian infectious laryngotracheitis viruses; and promoting in vitro, so that the avian infectious laryngotracheitis virus resisting specific transfer factor is obtained. The specific transfer factor prepared by the invention can be used for preventing and treating avian infectious laryngotracheitis, and meanwhile the transfer factor can be used for enhancing the immunity of chickens. The preparation method of the avian infectious laryngotracheitis virus resisting specific transfer factor, which is relatively ideal, has the advantages of being strong in specificity, low in cost, high in yield, good in effect and the like.

The invention relates to application of 3beta-acetyl oleanolic acid in preparing a drug for treating autoimmune diseases and belongs to the technical field of biomedicines. The 3beta-acetyl oleanolicacid can inhibit functions of a transcription factor RORgammat, differentiation of a Th17 cell, transcriptional expression of target genes IL-17A and IL-17F molecules of RORgammat in RNA level, secretion of an IL-17A cell factor and level of an anti-dsDNA antibody in blood serum, reduce the total quantity of IL-17A cells in spleen and reduce the renal degree of injury. The 3beta-acetyl oleanolic acid provided by the invention has an obvious function of inhibiting activity of the Th17 cell and provides a piloting structure for research and development of a new drug for treating autoimmune diseases.

The invention relates to the field of biotechnology, in particular to a recombinant expression vector, a host and a construction method thereof, and an animal model construction method. The recombinant expression vector consists of nucleotide for encoding soluble humanized HLA-A2 proteins and a prokaryotic expression vector. By virtue of the special prokaryotic expression vector, 'sufficient' soluble humanized HLA-A2 proteins can be synthesized; by taking the purified HLA-A2 proteins as an immunogen, the 'accuracy' of an experiment is guaranteed; the HLA-A2 proteins at different concentrations can promote a recipient mouse to generate preformed HLA-A2 antibodies at different titers, so that the generating strength of the preformed antibodies becomes 'controllable'; and the change of 'lymphocytes in marrow and spleen' of a preformed antibody animal model can be successfully observed.

The invention relates to a preparation method of an anti-duck hepatitis virus specific transfer factor. The method includes: inoculating a duck hepatitis virus to the allantoic fluid of a 9-11 days old chicken embryo or duck embryo to prepare a virus antigen, and extracting a duck hepatitis virus antigen; then taking chicken spleen or peripheral blood as the raw material to prepare a lymphocyte monolayer, then using phytohemagglutinin and the duck hepatitis virus to perform induction culture of lymphocyte, thus generating the anti-duck hepatitis virus specific transfer factor in vivo. The specific transfer factor prepared by the method provided by the invention can be used for prevention and treatment of duck hepatitis disease, and also can improve the immunity of ducks. The method provided by the invention has the advantages of strong specificity, low cost, high yield and good effect, etc., and is an ideal preparation method of duck hepatitis virus transfer factor.

The invention relates to an in-vitro preparation method of transfer factors capable of resisting duck viral hepatitis. In the method, the transfer factors capable of resisting the duck viral hepatitis are prepared through the induction of duck hepatitis virus by utilizing an in-vitro lymphocyte cell culture method; and chicken spleen or peripheral blood is used as a basic raw material to prepare single-layer lymphocyte cells, and then the single-layer lymphocyte cells are induced and cultured through phytohemagglutinin and the duck hepatitis virus, so that a large number of specific transfer factors capable of resisting the duck hepatitis virus are generated in vitro.. The transfer factors can effectively inhibit the virus of duck viral hepatitis, so that the normal bodies cannot be infected with the duck viral hepatitis, and the radical pre-prevention action can be realized. The method provided by the invention has the characteristics of being simple and easy to operate and the like.

Disclosed herein are methods and compositions comprising compounds capable of normalizing neuronal calcium dyshomeostasis. Also disclosed are methods comprising these compounds for treating neuronal or neurological disorders, including Alzheimer's disease, Parkinson's disease, Huntington's disease, fronto-temporal dementia, Pick's disease, chronic traumatic encepholopathy, traumatic brain injury, stroke, cerebellar ataxia, multiple sclerosis, Down syndrome, and aging-related CNS disorders.

The invention discloses health tea capable of adjusting the blood circulation of spleen. The health tea is prepared from red dates, angelica sinensis, fleece-flower root, liquorice, mulberry leaves and the like through crushing and baking. The health tea has the effects of enhancing the immunity of the organism, increasing coronary flow, softening blood vessels, lowering blood vessel resistance, regulating blood pressure and blood lipid and alleviating arteriosclerosis, has very great effects on the blood circulation and metabolism of the liver and spleen, has auxiliary treatment effect on patients with liver and spleen diseases, and arteriosclerosis, and can be used for early preventing the diseases.

The invention discloses an application of juglone to preparation of a medicament for treating autoimmune and inflammatory diseases. As proved by an experimental result, the juglone can be used for lowering the disease incidence, average clinical score, highest clinical score and total clinical scores of mouse experimental autoimmune encephalomyelitis, lowering the percentages of an IFN (Interferon)-gamma secretion-relevant cell Th1 and a 1L-17A secretion-relevant cell Th17 in spinal marrow of mouse experimental autoimmune encephalomyelitis in a CD4+T cell, inhibiting the proliferation of a cell T induced by MOG (Myelin Oligodendrocyte Glycoprotein) of the mouse experimental autoimmune encephalomyelitis, and lowering the expression of CD11b+ angtigen presentation cells CD83 and MHCII in the spleen of the mouse experimental autoimmune encephalomyelitis. The juglone for preparing a medicament for inhibiting an immune system related to the invention is expected to become a medicament for clinically treating autoimmune inflammatory diseases such as multiple sclerosis, acute disseminated encephalomyelitis and the like.

The invention discloses traditional Chinese medicine composition for treating the Alzheimer's disease as well as a preparation method and an application of the traditional Chinese medicine composition. The traditional Chinese medicine composition comprises milkvetch roots, Chinese angelica, red peony roots and prince's-feather fruits. Research indicates that the traditional Chinese medicine composition can improve the spatial learning ability and the spatial exploration ability of Kunming mice suffering from the Alzheimer's disease, increase visceral indexes of brains and spleens of the mice suffering from the Alzheimer's disease, reduce the permeability of Alzheimer's disease blood brain barriers, decrease Alzheimer's disease brain tissue hippocampal Abeta, IL-6 and IL-12 protein expression levels and have no obvious effect on weight of Alzheimer's disease models, namely, the Kunming mice. The results indicate that the traditional Chinese medicine composition is good in treatment effect on the Alzheimer's disease, so that the traditional Chinese medicine composition has the broad application prospect in the field of treatment of the Alzheimer's disease.