38 results about "Juglone" patented technology

The invention discloses a method for extracting juglone from walnut green husk residue by microwave reflux, which ueses chloroform solvent for extraction by a microwave reflux extraction method, preferably adopting walnut epicarpe (green husk) residue with higher juglone content and can be obtained easily as raw materials for extraction. The method is carried out under the optimal extraction condition that the solid-to-liquid ratio of walnut green husk with average grain size of 50 mesh to the chloroform solvent is between 1:5 and 1:8, and comprises the steps of: subjecting walnut green husk residue to extraction and pumping filtration at soft fire in microwave power level and washing the filter residue with solvent to obtain juglone extraction liquid, collecting all solutions, and preparing the juglone by decompression, rotary evaporation and concentration. After all index measurements, the extraction ratio of the juglone is 92%, the purity of product is 81.5%, and the product yield is 0.20%. The method is rapid and convenient, and has high extraction ratio, and devices in the method are easy to operate, have low energy consumption, consume less solvents which are easy to be recovered, and are suitable for the expanded production in factories. Meanwhile, the problem that juglone is heated and decomposed in a traditional method can be effectively avoided, which guarantees the effectiveness of extractives. The method satisfies the environmental protection and industrialization demands. The extracted juglone can be widely applied, and active ingredients in the juglone have the effects of antibacterial, anticancer and sedation of central nervous system, and have wide application prospects in such fields as medicine, pesticide, dye, pH indicator and the like.

The invention relates to an extraction method of chemical components in walnut green tangerine peel, particularly an extraction method of juglone and derivative in the walnut green tangerine peel. The extraction method comprises the following steps: pealing fresh walnut green tangerine peel, and sieving by a screen mesh of 40-80 meshes after washing, drying and crushing; putting the sieved walnut green tangerine peel powder into a container, and adding pure water according to a solid-liquid quality ratio of 1: (8-12); adding pectase and cellulase in the container, sealing the container after regulating the ph values of the solution in the container to 5.5-6.7, and then oscillating the resultant at the constant temperature of 20-40 DEG C for 36-60h; reducing pressure of and condensing the solution after enzymatic hydrolysis to obtain a concentrate; and extracting the resultant by petroleum ether after freezing and drying the concentrate to obtain juglone and derivative. In the invention, walnut green tangerine peel cell wall is destroyed by enzymatic hydrolysis, and recovery rate is raised by microwave ultrasonic wave processing; the technology of obtaining juglone and derivative by petroleum ether extraction is simple with high recovery rate, and the problems of low pericarp salvaging and low quinone substance recovery rate in walnut plucking and processing are solved.

Multifunctional compositions and methods are provided for therapeutic treatment of bacteria and cancers and for fluorescence diagnosis. Systems generate in situ reactive oxygen species such as singlet oxygen (1O2), hydroxyl radical (OH) and Juglone, and other chemotherapeutic agents. Methods provided selectively produce greater amounts of one reactive oxygen species over others. Variations are effective in aerobic, anaerobic or H2O2 rich environments and in presence of, or absence of, light. In H2O2 rich environment in absence of light, variations decompose H2O2 into O2 gas to remove excess H2O2 for elimination of hypoxic environment. Variations are formed of porphyrins, naphthalene derivatives, and metal ions, for illustration, free base tetrakis Ar substituted porphyrine core without metal or halide substitution but having hydroxyphenyl and alkyl pyridyl substituents at meso positions combined with dihydroxynaphthalene and +3 hydrated metal ions.

The invention belongs to the field of traditional Chinese medicine preparations, relates to a novel use of juglone having a structure shown in a formula (I) in pharmacy, and particularly relates to a use of the juglone in preparation of medicines treating brain tumor. In-vitro U87 spongioblastoma cell line killing tests and animal tests on a spongioblastoma model are performed. Results of the tests show that: the IC50 of the juglone in the killing tests is 6 [mu]g / mL; in the animal tests, the lifetime of a juglone group is longer than that of a contrast group; and by systematic antitumor medicine evaluation, the results prove that the juglone has significant inhibiting functions for the spongioblastoma in vitro and in vivo, and can be used for preparation of chemotherapeutics for treating the brain tumor especially the spongioblastoma. The formula (I) is shown in the specification.