190results about How to "Enhance humoral immunity" patented technology

The present invention is directed to novel adjuvant compositions, which target the cellular and / or humoral arms of immunity. According to an embodiment of the invention, an oil-in-water adjuvant composition is provided that comprises: (a) water; (b) a metabolizable oil comprising at least one branched or unbranched long-chain hydrocarbon moiety; (c) a steroid; and (d) a saponin.

The invention discloses a fusion protein of a novel coronavirus envelope protein and an application of the fusion protein. The fusion protein (SARS2-RBD-Fc) is obtained by fusing an RBD structural domain of a novel coronavirus envelope protein S with an antibody Fc fragment; and as a subunit vaccine, the fusion protein can induce an organism to generate an efficient neutralizing antibody through nasal drip immunization and intramuscular injection. It indicates that the SARS2-RBD-Fc can be used as a candidate vaccine for preventing and treating new coronavirus infection.

The invention provides a compound feed additive which is prepared by compounding hawthorn powder, portulaca oleracea herb micro powder, honeysuckle extract, dandelion extract, grape seed extract, echinacea extract, astragalus extract, common andrographis herb extract, numerous types of plant materials subjected to ultramicro grinding or extracting. The plant extract feed additive is prepared by selecting natural plants through the processes of micro grinding, extracting, concentrating and the like, and has the characteristics of wide raw material sources, high biological utilization rate, no toxicity and no residue, and antibiotics are prevented from being accumulated in the livestock body at the source. The compound feed additive can obviously reduce the piglet diarrhea which is caused by various reasons, the daily increased weight of the piglet is increased, and the humoral immunity and cell immunity functions of the piglet are improved.

The present invention relates to a streptococcus pneumoriae polysaccharide-outer membrane protein combined vaccine and its preparation method, belonging to the field of streptococcus pneumoriae vaccine. The main antigen component of said vaccine is a streptococcus pneumoriae capsular polysaccharide-outer membrane protein combined product obtained by covalently connecting the capsular polysaccharide produced by streptococcus pneumoriae with its outer membrane protein. Said capsular polysaccharide is the capsular polysaccharide of one or several kinds of streptococcus pneumoriae, its molecular weight is about 200-500 KDa, every polysaccharide molecule has about 300-700 repeating units. The outer membrane protein is the outer membrane protein of one or several kinds of streptococcus pneumoriae, its molecular weight is about 30-100 Kda. Said vaccine can be used for preventing or curing the diseases induced by streptococcus pneumoriae.

The invention belongs to the field of feed and in particular relates to a pig feed for the latter fattening period. The pig feed disclosed by the invention comprises corn flour, distillers' grains of Baijiu, wheat bran, soybean meal, peanut meal, peanut vine meal, sesame seed meal, rapeseed dregs, palm meal, rice bran, sweet potatoes, potatoes, sweet potato vine meal, brown seaweed, a complex enzyme preparation, a complex-probiotic agent, citric acid, sodium carboxymethyl cellulose, edible salt, fishbone dust, shell powder, calcium carbonate, lysine, methionine, ginger powder, premix, soybean dietary fiber flour, green feed and chitosan. The feed formula disclosed by the invention has the innovation that the green feed is added, so that the absorption and digestion ability of pigs in the latter fattening period is improved, the growth speed of the pigs is improved, and the disease resistance of the pigs in the latter fattening period is improved. In addition, a variety of feeds which are difficult to digest and absorb are fermented and transformed into components which are easy to digest and absorb, and the growth of the pigs in the latter fattening period is facilitated.

The invention belongs to the field of feed, and particularly relates to pig feed for the early stage of fattening. The pig feed comprises corn flour, beer vinasse, wheat bran, soybean meal, peanut meal, peanut vine bran, sesame seed meal, rapeseed meal, palm meal, rice bran, sweet potatoes, potatoes, sweet potato vine bran, brown seaweed, complex enzyme preparations, compound probiotic preparations, citric acid, sodium carboxymethylcellulose, salt, fishbone meal, shell powder, calcium carbonate, lysine, methionine, ginger powder, premix, soybean dietary fiber flour, green feed and chitosan. The pig feed has the advantages that the green feed is added into the formula of the feed innovatively, digestion and absorption capabilities of pigs in the early stage of fattening are improved, growth speed of the pigs is increased, and disease resistance of the pigs in the early stage of fattening is improved; various kinds of feed not easy to digest and absorb is fermented into ingredients easy to digest and absorb, and growth of the pigs in the early stage of fattening is facilitated.

The invention relates to a compound plant essential oil-type feed additive capable of replacing antibiotics. The additive is capable of regulating intestinal microbial floras, preventing bacterial diarrhea and respiratory diseases, increasing feed intakes, raising body health levels as well as improving animal production performances. The compound plant essential oil-type feed additive is prepared from the following ingredients: cinnamaldehyde, ginger essential oil, carvacrol, geranium essential oil, common origanum oil, folium eucalypti oil and carriers.

The invention discloses a preparation method of a complex live vaccine for porcine reproductive and respiratory syndrome, belonging to the technical field of veterinary biological products. The preparation method comprises the following steps: (1) culturing master cells; (2) performing suspension culture on vaccine preparation cells; (3) culturing and harvesting viruses; (4) performing mixed adsorption on the harvested full-suspension virus solution and chicken anti-porcine reproductive and respiratory syndrome virus egg yolk antibody; (5) adding the adsorbed antigen-antibody compound into an immunoenhancer-containing freeze-drying protecting agent, sub-packaging and then performing freeze vacuum drying to prepare the complex live vaccine for the porcine reproductive and respiratory syndrome. The method is used for preparing the vaccine, and has the characteristics that the production period can be shortened, the personnel allocation is reduced, the pollution probability is low, the floor area is small, the virus titer is high, the difference between the prepared vaccine batches is small, the product has stable and high-efficiency quality, the side effect is small and the quality of the vaccine can be completely improved.

A Chinese medicine as an immune regulator used for preparing the antineoplastic Chinese medicine is prepared from astragalus root and acanthopanax bark through decocting, concentrating, depositing inalcohol, and extracting from its deposit by alcohol. Its advnatage is high effect on improving immunity and playing its synergistic role in chemicotherapy and radiotherapy.

The invention provides medicinal liquor for improving immunity. The medicinal liquor is obtained by soaking the following raw materials in parts by weight in grain liquor: 1-20 parts of codonopsis pilosula, 1-20 parts of cornua cervi pantotrichum, 1-20 parts of astragalus mongholicus, 1-20 parts of pseudo-ginseng, 1-20 parts of rehmannia glutinosa, 1-10 parts of cordyceps sinensis, 1-10 parts of yam, 1-10 parts of medlar, 1-10 parts of ginseng, 1-10 parts of carthamus tinctorius, 1-10 parts of eucommia ulmoides, 1-10 parts of lucid ganoderma, 1-10 parts of gecko, 1-10 parts of red date, 1-10 parts of sea horse, 1-10 parts of cistanche, 1-10 parts of herba epimedii, 1-10 parts of radix polygonati officinalis and the like. The medicinal liquor has the advantages of remarkably improving the tumor weight inhibition ratio and the NK (Natural Killer) cell activity, reducing the TNF (tumor necrosis factor) alpha content in the serum and promoting the IFN (Interferon) gamma and IL-2 (interleukin-2) contents to rise, so that the medicinal liquor is capable of boosting the humoral immunity and cell immunity of the human body with remarkable effect; and at the same time, the medicinal liquor is obtained by soaking natural animal and plant ingredients as well as pure traditional Chinese medicine materials, so that the color of the liquor is natural amber, no toxic or side effects are caused and the preparation is convenient.

The invention belongs to the technical field of traditional Chinese medicine moxibustion, and particularly relates to a far infrared medicinal moxibustion substrate, a far infrared medicinal moxibustion product containing the far infrared medicinal moxibustion substrate and a preparation method of the far infrared medicinal moxibustion product. The far infrared medicinal moxibustion substrate mainly comprises the following raw materials, by weight, including 150-250 parts of bee wax, 30-50 parts of chocolate, 10-30 parts of honey, 100-200 parts of far infrared ceramic powder volcano energy mud, 20-40 parts of agaropectin, 16-18 parts of vaseline and 2-4 parts of capsaicin. The far infrared medicinal moxibustion product mainly comprises the following raw materials, by weight, including 1 part of the far infrared medicinal moxibustion substrate, 7-9 parts of ginseng, 5-7 parts of saffron crocus, 6-8 parts of rhodiola rosea, 8-10 parts of herba cistanche and 9-11 parts of dioscorea nipponica makino. The preparation method of the far infrared medicinal moxibustion product comprises the following steps of preparation of the far infrared medicinal moxibustion substrate, preparation of a drug mixture and preparation of a finished product; the preparation method is simple, convenient to use, relatively low in cost, and beneficial to popularization and application.

The invention provides a heat-resistant freeze-drying protective agent applied to animal freeze-dried live vaccine. The heat-resistant freeze-drying protective agent comprises 1%-3% of glutin, 1%-8% of lactose and the like, wherein, all substances are dry components, and distilled water takes all the rest percentage. The invention also provides a method for preparing the protective agent. ND live vaccine prepared by the protective agent of the invention can be conserved for more than 24 months at the temperature of 2 DEG C to 8 DEG C. Immunity test shows that the average antibody level of immunized chicken is 1 to 2 titers higher than the average antibody level of tested chicken immunized by the ND live vaccine which is freeze-dried by ordinary protective agent, and the antibody level is uniform. Therefore, the freeze-drying protective agent can be widely applied to the production and conservation of various animal live vaccines and has wide application prospect.

The present invention proposes one kind of organic composite Chinese medicine immunopotentiator / adjuvant for preventing and treating animal contagious diseases and its preparation process. The Chinese medicine immunopotentiator / adjuvant is prepared with propolis and eight kinds of Chinese medicinal materials, including astragalus root, fortune euonymus stem or leaf, Dangshen, glossy ganoderma, ginseng, etc. and through compounding the extracted liquid of the Chinese medicinal materials and the alcohol leaching liquid of propolis. It can enhance the immunity of available vaccine and strengthen animal's disease resistance.

The present invention relates to a microorganism source liver-protecting natural nutrient health care product and a preparation method thereof, which is characterized in that prebiotics and fungi strains which are allowed to be used by National Drugs Supervision Bureau are adopted; the traditional Chinese medicines that are normally used and the health care foods of microorganism source can produce a great deal of active protective protein and are taken as raw materials to carry out compound ferment; the product contains a great deal of active nutrient components which can improve detoxification and oxidation resistance capability of the liver. The present invention provides a biological anti-oxygen source nutrient product which is produced by fermenting microorganism and is a green health care product enriched in renieratene, B vitamins, reduction type vitamin C, queretin-3-D glucopyranose (quercitrin), queretin (flavonoid), phaseomannite and the metal derivative with various microelements, so as to obviously improve the restoring function of human liver after being damaged, enhance the ability of organism for eliminating free radical, reduce the damage on organism caused by the free radical, thus being a safe green liver-protecting natural nutrient health care product.

The invention relates to a compound preparation for treating the virosis of birds and the preparation method of the compound preparation. The compound preparation is prepared by mixing raw materials by weight portions, 20 to 50 parts of milk veteh and 30 to 50 parts of grifola frondosa. Preparation procedures are as follow: The raw materials are extracted by soaking, decocted by heating and precipitated by filtering; therefore the Chinese herbal medicine is prepared. The compound preparation has the beneficial effect as follows: (1) the new preparation that can control the virosis of the birds and strengthen the disease resistance of organism is created for clinical treatment; (2) the compound mode is reasonable, the antiviral capability is strong and all the selected medicine is the effective medicine for treating the virosis of birds; (3) the compound preparation has the advantages of advanced technology, stable preparation and long storage life; (4) the compound preparation has the advantages of being safe to use, definite healing effect and controllable quality; (5) the compound preparation has the advantages of wide source of the raw materials, being avirulent, no side effect and being uneasy to cause drugfastness, so as to meet the standard of international hygienic examination. The compound preparation is especially applicable for treating infectious bursal disease, atypical newcastle disease and infective bronchitis, and the treating effect is remarkable.

The invention relates to a traditional Chinese medicine feed addictive for controlling the degradation of the laying rate of chicken, which is powder prepared from the following raw materials in parts by weight: 4-6 parts of dyer woad leaf, 4-6 parts of cyrtomium fortunei, 7-9 parts of leonurus heterophyllus, 2-4 parts of fructus ligustri lucidi, 2-4 parts of fllase asiabelI root, 2-4 parts of astragalus root and 1-3 parts of liquorice. The traditional Chinese medicine feed addictive is prepared by crushing and mixing the raw materials. The invention is used for controlling the degradation of the laying rate of chicken, has favorable effect, and also has the characteristics of convenient use, safety and no medicine residue.

Modified HIV Env, Gag, Pol, or Nef DNA with improved ability to elicit antibody and CTL responses to HIV antigens have been identified as prototype immunogens for the treatment and prevention of HIV infections.

The invention relates to a Chinese medicine composition with the function of immunological enhancement and application thereof. The Chinese medicine composition comprises the following Chinese medicines in parts by weight: 200-400 parts of Astragalus membranaceus, 100-300 parts of Chinese angelica, 100-300 parts of epimedium herb, 100-200 parts of Chinese wolfberry fruit, 100-200 parts of Chinese atractylode rhizome and 100-200 parts of hawthorns. Animal experiments prove that the Chinese medicine composition can obviously improve the cellular immunity function and the humoral immunity function of livestock and poultry to promote growth, obviously promote lymphopoiesis in vitro and has no toxicity and clinical application safety. The composition disclosed by the invention has the advantages of high efficiency, safety, quality controllability and the like.

The invention discloses an application of a yeast dextran injection for improving the immune efficiency of a foot-and-mouth disease vaccine of a dairy cow. Simultaneously, the invention also discloses an application of the yeast dextran injection for enhancing the cellular immune function of the dairy cow, wherein the yeast dextran injection is a 2%-6% carboxymethyl yeast dextran water solution. The yeast dextran injection prepared by the invention is safe, does not have adverse reaction, can effectively enhance the cellular immune function of the dairy cow organism and improve the antibody level of the dairy cow inoculated with an foot-and-mouth disease inactivated vaccine, and can enhance the disease resistance of animals.

The present invention relates to a preparation method of polyporus polysaccharide sulfate. Said method includes the following steps: (1). preparing esterification reagent; (2). selecting and using polyporus polysaccharide powder and anhydrous methanamide or dimethyl methanamide according to mass ratio of 0.4-2.0:36-48, making the polyporus polysaccharide powder be suspended in anhydrous methanamide or dimethyl methanamide to obtain polyporus polysaccharide suspension; (3). making esterification reaction; (4). precipitation; (5). purifications and (6). secondary precipitation. Besides, said invention also provides the concrete requirement of every step.

The invention discloses a composition for strengthening the immunity function of an organism and a preparation method thereof. The composition is mainly prepared from the following raw materials in parts by weight: 67-201 parts of glossy ganoderma spore powder, 33.5-100.5 parts of astragalus membranaceus extracts, 25-75 parts of glossy privet fruit extracts, and 17.5-52.5 parts of turmeric extracts. A modern preparation technology is used, and an advanced quality control method is used, so that products are safe, stable and controllable. Animal experiments prove that compared with a preparation made of single glossy ganoderma spore powder and a preparation made of single astragalus membranaceus extracts, the composition disclosed by the invention has more excellent effects of strengthening the immunity of the organisms under the condition of the same dosage, and in addition, the composition does not have any toxic or side effects, so that the composition has higher development and utilization values and market application prospects.

The invention discloses carp feed which comprises soybean meal, yellow soybean meal, black soybean powder, wheat flour, millet flour, glutinous millet flour, polished glutinous rice flour, corn flour, rice bran powder, oat powder, sesame powder, linseed powder, egg shells, crab shells, chicken breast, sunflower seeds, rapeseed, fish oil, spirulina, seaweeds, porphyra tenera, carrots, composite trace elements, citric acid, lactic acid, oyster powder, fish meal, glutathione, phospholipid, vitamin C, vitamin E, vitamin A, calcium gluconate, zinc gluconate, acetic acid, DHA (docosahexaenoic acid), plant enzyme and algin. The fish feed disclosed by the invention is rich in nutrition and enriched in various amino acids, proteins and microelements, meanwhile, a variety of organic acids and enzymes are enriched, so that the survival rate of fishes is improved, and the meat quality of fishes can be effectively improved.

The invention relates to a Chinese herbal medicine oral liquid for treating piglet diarrhea. The Chinese herbal medicine oral liquid is prepared through the steps: by taking 40-60 parts by weight of dried medicinal material downy rosemyrtle root and 20-30 parts by weight of dried medicinal material herba fibraureae recisae, obtaining a downy rosemyrtle root extract through poach extraction, gradient alcohol precipitation and drying, obtaining a coarse herba fibraureae recisae product through percolation extraction, precipitation and drying, and adding water to the downy rosemyrtle root extract and the coarse herba fibraureae recisae product to prepare the Chinese herbal medicine oral liquid. The Chinese herbal medicine oral liquid for treating piglet diarrhea is wide in raw material source, simple and easy in production process, low in energy consumption, easy to control in quality, stable in quality of product, and applicable to large-scale production.

The present invention discloses a processing method of health-care muraenesox cinereus. The processing method includes 6 steps. By increasing a processing step 5 of vacuum infiltration, muraenesox cinereus blocks and pre-wine lee pickling essence are mixed, and the mixture is subjected to vacuum infiltration in a vacuum infiltration machine. Related seasoning materials and sorghum wine are added into the pre-wine lee pickling essence used in the step 5 of the vacuum infiltration, which increases the fresh and sweet tastes of the wine lee pickled muraenesox cinereus products and enables the muraenesox cinereus blocks to be compact in meat quality, full of elasticity, fresh, tender and delicious, appropriate in saltiness and unsaltiness and mellow in aroma, wine fragrance and fish flavor. By a step 7 of packaging and sterilizing, the wine lee pickled muraenesox cinereus mature products are subjected to sterilization which can prevent an excessive fermentation of the products and can also improve the shelf life of the products. 2-5% of bulbus lilii and 3-8% of mulberries are added into the pre-wine lee pickling essence in the step 5 of the vacuum infiltration and the mixture is boiled into soup by adding water, so that the health-care function of the pre-wine lee pickled muraenesox cinereus is increased. The wine lee pickled muraenesox cinereus products obtained by the above method have efficacies of supplementing deficiency, tonifying blood, removing dampness, strengthening spleen and stomach, and aiding digestion.

The invention discloses an alga oligosaccharide fish feed additive and relates to an alga oligosaccharide. Aiming at solving the problems of the prior art, the invention provides the alga oligosaccharide fish feed additive. The alga oligosaccharide fish feed additive improves a feed utilization rate, promotes growth, improves disease resistance, reduces a cost and improves fish quality. The alga oligosaccharide of the alga oligosaccharide fish feed additive is gracilaria lemaneiformis agar oligosaccharide and is a mixture which comprises tetrasccharide to octasaccharide and is obtained through co-culture of a Flammeovirga Pacifica H2 strain and gracilaria lemaneiformis so that gracilaria lemaneiformis is directly degraded through an enzyme produced through microorganism. The Flammeovirga Pacifica H2 has a preservation number of CCTCC NO: M2012229.

The invention discloses a Chinese medicinal herb-containing feed additive capable of preventing weaned pig multisystem failure syndrome and pig feed containing the Chinese medicinal herb-containing feed additive. The Chinese medicinal herb-containing feed additive comprises the following ingredients: root of hairy asiabell, bighead atractylodes rhizome, tuckahoe, Chinese yam, radix astragali, white hyacinth bean, coix seed, lotus seed, fructus amomi, dried tangerine or orange peel, parched medicated leaven, parched malt, parched hawthorn fruit, Platycodon grandiflorum, lily, radix stemonae, Polygonum multiflorum, herba epimedii, glossy privet fruit, herba ecliptae, Coptis chinensis, rhizoma picrorhizae, the fruit of Rangoon creeper and liquorice. The Chinese medicinal herb-containing feed additive is 1.5-2wt% of the pig feed, can improve immunity of the weaned pig, prevent weaned pig multisystem failure syndrome, and has the characteristics of cheap price, non-toxic effect and non-side effect.

Catfish feed is disclosed. The feed includes soybean powder, soybean powder, black bean powder, wheat flour, millet powder, broomcorn millet flour, flour of polished glutinous rice, corn flour, rice bran powder, oat meal, sesame powder, linseed meal, eggshell, crab shells, chicken breast, sunflower seed, rapeseed, fish oil, carrots, compound trace elements, citric acid, lactic acid, oyster powder, fish meal, glutathione, phospholipid, vitamin C, vitamin E, vitamin A, calcium gluconate, zinc gluconate, acetic acid, DHA, plant enzymes, and algin. The feed is rich in nutrition, rich in a plurality of amino acids, proteins and trace elements, and rich in a plurality of organic acids and enzymes, increases the survival rate of fishes and effectively improves meat quality of fishes.

The instant invention comprises a process for selecting and manufacturing antibodies useful for therapeutic, prophylactic, diagnostic or research purposes using epitope peptide mixtures synthesized by the solid phase synthesis, such process defined by a set of rules regarding the identity and the frequency of occurrence of amino acids that substitute a base or native amino acid of a known epitope. The resulting antibodies are related to but distinct from antibodies that bind to the known epitope.

A refractory immunopotentiator for the freeze-dried living vaccine of animal in order to elongate its storage period to more than 24 months at 2-8 deg.C and improve its immunizing level and power is proportionally prepared from gelatin, VC, lactose, beef peptone, PVP, sorbitol, sodium glutamate, bovine serum albumin, EDTA, compound cell peptide and distilled water through high-pressure removal of bacteria, filtering and proportional mixing.

The invention belongs to the field of biotechnology and particularly relates to a schistosoma japonica vaccine salmonella secretion expression vector, which is a recombinant salmonella expression vector that is controlled by a bacterial promoter, guided by effector protein secreted by salmonella and secretion signal of the effector protein and used for secretion expression of schistosoma japonica antigen. The schistosoma japonica vaccine salmonella secretion expression vector can induce secretion expression of a schistosoma japonica vaccine in a hypoxic microenvironment in antigen presenting cells, can exist in vivo stably and continuously and is insusceptible to being lost. The schistosoma japonica vaccine salmonella secretion expression vector, by using an attenuated strain as a carrier, can perform the antigen carrying of the recombinant vaccine representing bacteria by means of oral taking. The schistosoma japonica vaccine salmonella secretion expression vector can be used for preparing schistosoma japonica vaccine for oral taking for preventing and treating schistosomiasis.