The invention relates to the technical field of organic synthesis, in particular to parthenolide derivatives, and a preparation method and application thereof. The 11 parthenolide derivatives are synthesized by taking feverfew as an initial main raw material through a series of chemical reactions, and the derivatives have certain anti-proliferative activity on glioblastoma cells. It is also provedin the invention that the deuterium-containing derivative DMAPT-D6 can obviously induce accumulation of active oxygen in glioblastoma cells, thereby leading to DNA damage in the glioblastoma cells. Furthermore, the DMAPT-D6 promotes death receptor-mediated exogenous apoptosis dependent on cysteine aspartase, indicating that DNA damage induced by the DMAPT-D6 can induce apoptosis of the glioblastoma cells. Thus, ROS accumulation caused by DMAPT-D6 treatment causes DNA damage, and then death receptor-mediated apoptosis is caused, so it is proved that the DMAPT-D6 with novel composition has treatment potential for treating glioblastoma and can be applied to preparation of medicines for treating glioblastoma.