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Application of ciclopirox olamine in preparation of medicine for treating tumor, composition medicine with ciclopirox olamine and application

A technology of ciclopirox olamine and its composition, which is applied in the preparation of drugs for treating tumors, the field of ciclopirox olamine

Active Publication Date: 2019-10-22
WENZHOU MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in the prior art, there is no research report on the sensitivity of tumor cells to ciclopirox olamine when bortezomib and ciclopirox olamine are combined to treat tumors.

Method used

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  • Application of ciclopirox olamine in preparation of medicine for treating tumor, composition medicine with ciclopirox olamine and application
  • Application of ciclopirox olamine in preparation of medicine for treating tumor, composition medicine with ciclopirox olamine and application
  • Application of ciclopirox olamine in preparation of medicine for treating tumor, composition medicine with ciclopirox olamine and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Example 1 Effect of ciclopirox olamine on the survival rate of cultured glioblastoma cells

[0037] 1. Cell Culture

[0038] The medium used for normal culture of glioblastoma cells U118, U251 and SF126 is DMEM medium containing 1% double antibody (penicillin / streptomycin) and 10% FBS. The amount of medium used is 7 mL for a 10 cm dish, 2.5 mL for a 6 cm dish, 2 mL for a 6-well plate, and 0.1 mL for a 96-well plate. The cells were cultured in a 37°C, 5% CO2 incubator, and an appropriate amount of autoclaved water was placed in the incubator to maintain a certain humidity. The confluence of cell growth should be less than 95%, otherwise it needs to be subcultured or frozen.

[0039] 2. Cytotoxicity experiment

[0040] (1) Cell plating: After U251, SF126 and U118 cells were cultured in the culture dish for 48 hours, the cells were in good condition under the microscope, and the cell density was about 80%. The original medium was discarded, and 2 mL of PBS was added to ...

Embodiment 2

[0052] Example 2 Drug efficacy experiment of ciclopirox olamine acting on glioblastoma xenograft tumor in nude mice

[0053] (1) Expansion of cultured cells: Human glioblastoma cells U251 and U118 were expanded until the desired number of cells was reached.

[0054] (2) Construct a tumorigenic model in nude mice: digest U251 and U118 cells, collect and count the cell pellets, resuspend the pellets with serum-free DMEM medium, and add an equal volume of serum-free DMEM medium to the cell pellets after counting Matrigel was used to make cell suspension, and each 5-week-old male nude mouse was subcutaneously injected with 150 μL containing 1×10 8 cell suspension of cells.

[0055] (3) Observation and grouping: measure the transverse and longitudinal diameters of the transplanted tumors with a vernier caliper, and wait until the tumor volume (volume V=1 / 2×a×a×b, where a is the transverse diameter and b is the longitudinal diameter) grows to 75-100 mm 3 The nude mice were randoml...

Embodiment 3

[0059] Example 3 The effect of combined use of ciclopirox olamine and bortezomib on glioblastoma cells

[0060] 1. Cell culture, as in the cultivation method in embodiment 1.

[0061] 2. Cell Proliferation Experiment

[0062] (1) Cell plating: U251 and SF126 cells were digested and counted, respectively inoculated into 96-well plates at a density of 2×10 3 cells / well, medium 0.1mL / well, culture overnight in a cell culture incubator.

[0063] (2) Cell dosing: After the cells adhere to the wall, add the medium containing the corresponding medicine, and keep away from light when adding the medicine. The drug concentrations used for U251 and SF126 were 0 μM, 20 μM CPX, 20 μM CPX+24nM BTZ, and 24nM BTZ, respectively. Six replicate wells were set for each concentration, and five time points were determined in total: 0h, 12h, 24h, 36h, and 48h.

[0064] (3) Detection of cell proliferation with MTT cytotoxicity and cell proliferation kit: the method is the same as the cytotoxicity ...

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Abstract

The invention discloses application of ciclopirox olamine in preparation of a medicine for treating glioblastoma multiforme and provides a composition medicine. The composition medicine comprises theciclopirox olamine and bortezomib. Through the bortezomib, the sensibility, of resisting growth of tumor cells, of the ciclopirox olamine is improved, particularly, the sensibility, of resisting growth of glioblastoma multiforme cells, of the ciclopirox olamine is improved, the ciclopirox olamine and the bortezomib are in combined utilization and can be used as a medicine for tumor-resistance treatment and particularly used as a medicine for glioblastoma multiforme resistance treatment, and accordingly the medicine tolerance of the ciclopirox olamine as the medicine for treating the tumor is reduced.

Description

technical field [0001] The invention relates to the field of tumor medicines, in particular to the application of ciclopirox olamine in the preparation of medicines for treating tumors and related combined medicines and uses. Background technique [0002] In recent years, the incidence of intracranial tumors is on the rise. According to statistics, intracranial tumors account for about 5% of systemic tumors and 70% of childhood tumors. Regardless of benign or malignant brain tumors, they can squeeze and push normal brain tissue, causing increased intracranial pressure and threatening human life. Among them, glioblastoma is the most common primary malignant tumor in the brain, mostly showing invasive growth. At present, the main treatment methods for glioblastoma are surgery, adjuvant radiotherapy and chemotherapy, but the prognosis is poor, the recurrence rate is high, the median survival time is only 14.6 months, and the growth is infiltrative. Most patients cannot be trea...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/4412A61P35/00A61K38/05
CPCA61K31/4412A61P35/00A61K38/05A61K2300/00
Inventor 吕斌韩胜男上官福根
Owner WENZHOU MEDICAL UNIV
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