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Colorectal cancer early hepatic metastasis animal model and construction method and application thereof

A technology of colorectal cancer and animal models, applied in the direction of biochemical equipment and methods, applications, and medical raw materials derived from mammals, etc., can solve the problems of high incidence of intestinal obstruction, short survival time of models, and inability to adapt to long-term experiments, etc. question

Inactive Publication Date: 2017-04-26
HARBIN MEDICAL UNIVERSITY
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Problems solved by technology

[0003] The purpose of the present invention is to provide an animal model of early liver metastases of colorectal cancer and its construction method and application, aiming at solving the problems of high incidence of intestinal obstruction, short survival time of the model and inability to adapt to long-term development of colorectal cancer liver metastases. The Problem with the Flaws of Time Experiments

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  • Colorectal cancer early hepatic metastasis animal model and construction method and application thereof
  • Colorectal cancer early hepatic metastasis animal model and construction method and application thereof
  • Colorectal cancer early hepatic metastasis animal model and construction method and application thereof

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[0027] In order to make the object, technical solution and advantages of the present invention more clear, the present invention will be further described in detail below in conjunction with the examples. It should be understood that the specific embodiments described here are only used to explain the present invention, not to limit the present invention.

[0028] The application principle of the present invention will be described in detail below in conjunction with the accompanying drawings.

[0029] Such as figure 1 As shown, the method for constructing an animal model of early liver metastasis of colorectal cancer provided by the embodiments of the present invention includes the following steps:

[0030] S101: Select human colorectal cancer cell lines respectively, and finally determine that the concentration of the cell suspension is about 2.5×10 7 / ml, slowly inject 0.1ml of colon cancer cells through the spleen, and the injection time is about 3 minutes;

[0031] S10...

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Abstract

The invention discloses a colorectal cancer early hepatic metastasis animal model and a construction method and application thereof. Cell suspension concentration of the colorectal cancer early hepatic metastases animal model is 2.5*107 / ml, about 0.1ml of colorectal cancer cells are injected intrasplenically for 3min; a Vetbond 3M tissue adhesive is adopted for sealing an incision, and then the spleen is put back. The colorectal cancer early hepatic metastases animal model in different metastatic characteristics is constructed innovatively; differences of in-vitro nuclein uptake ratios of cancer cells in different metastatic potentials are verified; statistic differences of biological characteristic indexes of different cancer strains and metastatic nodules are found; early hepatic metastatic tumor development biological characteristics are reflected completely, and a novel method is provided for discovery and diagnosis of early hepatic metastasis.

Description

technical field [0001] The invention belongs to the technical field of colorectal cancer, and in particular relates to an animal model of early liver metastasis of colorectal cancer and its construction method and application. Background technique [0002] At present, the implantation model of colorectal cancer liver metastases has a high incidence of intestinal obstruction, short survival time of the model, cannot adapt to long-term experiments, fails to simulate the whole process of tumor metastasis, does not conform to the clinical characteristics of the mechanism of liver metastases, and has a relatively long induction cycle. long-term defects. Contents of the invention [0003] The purpose of the present invention is to provide an animal model of early liver metastases of colorectal cancer and its construction method and application, aiming at solving the problems of high incidence of intestinal obstruction, short survival time of the model and inability to adapt to l...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61D7/00A61D1/00A61B8/00A61B6/03A61B5/055C12Q1/02A61K35/13G01N1/30G01N21/25A01K67/027
CPCA01K67/027A61B5/055A61B6/03A61B6/481A61B6/508A61B8/00A61B2503/40A61D1/00A61D7/00A61K35/13G01N1/30G01N21/25G01N2001/305
Inventor 姜慧杰闫林林姜昊徐海龙李鑫
Owner HARBIN MEDICAL UNIVERSITY
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