The invention discloses a preparation method and applications of a young repair-type fibroblast, and belongs to the technical field of cell biology. The JAK-STAT signal path in the repair-type fibroblast provided by the invention is inhibited, and the cell has the characteristic of being young. The preparation method adopts a small molecular compound, a cytokine combination or a recombinant protein combination to inhibit a corresponding signal path in a common fibroblast, and can be used for the reprogramming of cells, tissues, organs and organisms or the rejuvenation of cells, tissues, organsand organisms. The repair-type fibroblast and a cell product thereof can be used for constructing tissue engineering materials, repairing the damage of mammalian tissues and organs and repairing aging and degenerative tissues and organs, delaying or reversing the aging process of cells, tissues, organs and organisms, and can be used for the immunoregulation of cells, tissues, organs and organisms. The repair-type fibroblast prepared by the technology has the characteristics of skin fibroblasts, and has the characteristics of mesenchymal stem cells, so the fibroblast is named as the repair type skin fibroblast.