The invention relates to a traditional Chinese medicine composition for treatment of chronic kidney failure and a preparation method, a preparation and use thereof, and belongs to the field of clinical treatment of traditional Chinese medicines. The traditional Chinese medicine composition for treatment of chronic kidney failure includes the following four groups of raw materials by weight: an A group selected form 10-25 parts of torrefied rhubarb and / or raw rhubarb; a B group selected form 11-18 parts of salvia miltiorrhiza and / or cortex moutan; a C group selected form 11-18 parts of red peony root and / or cortex moutan; and a D group including 10-14 parts of Chinese angelica, 25-50 parts of raw bone fossil of big mammals, 25-50 parts of raw oysters and 10-20 parts of dandelion. The traditional Chinese medicine composition used in treatment can improve clinical symptoms, reduces serum creatinine, increases endogenous creatinine clearance rate, increases the content of hemoglobin, achieve the curative effect in the treatment of chronic renal failure and complications thereof, delays the progression of the chronic renal failure, and protects the residual renal function, delays dialysis of patients, improves the survival rate and life quality of the patients, reduces medical expenses, reduces family and society burden, and is worthy of popularization and application.