115results about How to "Free from infection" patented technology

The invention discloses a grouper large scale total-artificial breeding method. The method comprises the steps of parent cultivation, artificial reproduction, offspring seed production, leaving pond of offspring seeds, and temporary rearing in a net cage. The grouper large scale total-artificial breeding method is suitable for seedling cultivation of orange-spotted groupers, or saddletail groupers, or giant groupers, or Hong Kong grouper, or East Star Groupers. The grouper large scale total-artificial breeding method solves the problem that the death rate of groupers in a current seedling cultivation technology is high, and broad prospect of scale development of grouper cultivation is achieved.

A milk substitute of calf for promoting growth, improving digestion and preventing diseases is prepared from 16 raw materials including corn, cotton seed cake, lysine, methionine, BFAB02, edible salt, stone powder, etc through respective pulverizing, proportional mixing, and stirring.

The present invention is applicable to the field of computer security, and provides a BIOS update protection method, device and system. The method includes: when the device is in the power-on self-test POST stage, after the programmable logic device is started, there is only one output The control signal controls the write protection pin of the ROM chip storing the BIOS to be in the write protection invalid state or the write protection effective state; when the BIOS update is not required and the write protection pin of the ROM chip is in the write protection effective state, the device enters operation The system prohibits BIOS updates through hardware protection. The write protection state of the ROM chip of the present invention can only be modified once in the power-on self-check state, and when the BIOS update is not needed, the BIOS program is protected from virus infection through hardware, effectively improving the safety of equipment operation.

The invention provides a method for preparing a biological bactericide for fruit trees by utilizing propolis and beewax. The method comprises the following steps of (1) soaking original raw glue or covering cloth for two times with edible ethanol, merging the soak solutions, adding CH3COOH solution for precipitation to remove lead, and filtering, then carrying out concentration in a vacuum concentration tank, controlling the material temperature, interlayer water temperature and atmospheric pressure of the tank and braising extract-shaped propolis in the tank after concentration is completed to obtain refined propolis; (2) smashing the refined propolis into small pieces and adding alcohol to dissolve the small pieces of refined propolis to prepare propolis solution; (3) decontaminating, drying and grinding eugenia caryophyllata, amur cork tree bark and rhizoma polygonati, mixing the powder according to parts by weight, extracting the mixture with alcohol according to the volume ratio and filtering the solution to obtain traditional Chinese medicine solution; (4) firstly melting lard, secondly stirring beewax and liquid paraffin in a stirrer and controlling the temperature and the rotating speed, thirdly adding and stirring the molten lard and propolis solution and controlling the temperature, rotating speed and time and fourthly adding and stirring the traditional Chinese medicine solution, stopping heating after a certain time, cooling the mixture while stirring so as to obtain the biological bactericide after complete cooling. The biological bactericide has the function of preventing fungi diseases of fruit trees and has the effects of improving the fruit quality and increasing the fruit yield.

The invention relates to cotton gauze sanitary towel which is immersed in or sprayed with Chinese medicinal herbal plant liquid. The Chinese medicinal herbal plant liquid for immersing or spraying the sanitary towel is prepared from Chinese medicinal herbal raw materials such as nacre, polygonum paleaceum wall, garden burnet root, common ducksmeat herb, cyrtomium rhizome and the like and water according to a ratio; and the cotton gauze sanitary towel is immersed in or sprayed with the Chinese medicinal herbal plant liquid, and after liquid medicine is absorbed by the sanitary towel fully, the sanitary towel is dried and thus, the Chinese medicinal herbal plant liquid cotton gauze sanitary towel is obtained. The Chinese medicinal herbal plant liquid cotton gauze sanitary towel has the effects of resisting bacteria, disinfecting, killing insects, relieving itching, dissolving stasis, and relieving pain, clearing heat, detoxicating, activating meridians and promoting blood circulation, regulating qi and blood, smoothing menstrual period and the like, can balance the pH value of female vaginae effectively and inhibit bacterial breeding, and has the excellent treatment effect of abnormal menstruation, dysmenorrheal and pudendal pain, pruritus vulvae, swelling, pudendal hygro-erosion, peculiar smell and the like which are caused by multiple vulvar diseases. Except for the menstrual period, the Chinese medicinal herbal plant liquid cotton gauze sanitary towel also produces the obvious curative effect on various common gynecological diseases when worn at ordinary times, and achieves the effects of treatment with external application and positive prevention.

The invention provides a liquid woundplast and a preparation method thereof, and belongs to the field of medical supplies. The liquid woundplast is prepared by mixing nitrocellulose, castor oil, hydrogenated castor oil, dioctyl phthalate, phenylcarbinol, ethyl acetate, butyl acetate and isopropyl alcohol and then adjusting a pH value to 6-8. After the prepared liquid woundplast is applied on a wood surface, the liquid woundplast can rapidly form a film in 1-2 min, thereby effectively isolating invasion of moisture, dust, dirt and bacteria, protecting wounds against infection and accelerating healing; and the liquid woundplast is soft, smooth, good in fitting property and not easy to fall off.

It is a process method of hemolysin in special cell device and its application, which belongs to medical test agent technique field. The hemolysin is composed of NaCI, Na#-[2] HPO#-[4]íñ12H#-[2] O, KH#-[2] PO#-[4], cavaform and double stilled water. Each component is mixed and is put in the water for four to five hours and is shaken every thirty to forty minutes and to be filtered to get the agent. The hemolysin in this invention is used as surface marks of leucocyte in blood tested by the flow cell device.

The invention belongs to the field of prevention and treatment of crop disease damage and particularly relates to a compound composition for biologically preventing and treating crop wilt and application thereof. The compound composition for biologically preventing and treating crop wilt comprises an organic fertilizer and strain powder having a weight ratio of 100:1 to 20:1. The compound composition is applicable to various crops with high wilt attack frequency in agricultural planting, such as common banana and dwarf banana. The compound composition for biologically preventing and treating crop wilt and application thereof have the advantages that the cost is low, the prevention and treatment stability is good, disease resistance rarely occurs, the advantages of biological prevention andtreatment are made full use, prevention and treatment effect is improved, daily management for crop cultivation is improved, and economic loss and prevention and treatment cost are reduced.

The invention discloses a preparation method for transferring gene of a natural effective pure biological active matter for resisting a newcastle disease virus. The invention is carried out according to the following steps: inoculating the newcastle disease virus into a chick embryo allantois liquid of ten days to prepare a virus antigen; extracting the virus antigen; using the extracted virus antigen for immunizing a pig; extracting the transferring gene for resisting the newcastle disease virus from the immunized pig; therefore, the transferring gene of the invention can be used for playing the role of preventing the infection of the newcastle disease virus and protecting a normal organism cell from being infected by the newcastle disease virus, thereby reducing the disease rate.

The invention provides a novel plant virus inoculation method, and in particular provides a field large-scale virus inoculation method by using a bacterium, mainly targeting at infectious clone of a plant virus which can be replicated in agrobacterium. The principle of the method is as follows: infectious clone of the plant virus is transformed into agrobacterium, and the virus can be brought into a plant cell by using the characteristic of natural wound and wound plant infection of the agrobacterium. The method is simple to operate, low in cost, high in efficiency, and suitable for field large-scale application, and can be used for the inoculation of a weak virus strain of the virus to protect a plant from the infection by a strong virus strain, and heterologous protein with high added value can be expressed in the plant using a virus vector.

The invention relates to a breeding method of a specific pathogen free (SPF for short) reed vole, which comprises the following steps in sequence: (1) breeding a male reed vole and a female reed vole as a pair in each cage and making the female reed vole pregnant; (2) determining the laparotomy time of the pregnant reed vole through palpation and observation of the pregnant reed vole; (3) disinfecting to prepare for laparotomy and extraction; (4) carrying out laparotomy and extraction for the reed vole; (5) breeding the newly born reed vole with a SPF CR mice within 1-5 day(s) and breeding the newly born reed vole with a SPF SD rat within 6-15 days; and (6) carrying out microbiologic and parasite detection for items necessary to be detected in GB14922.1-2001 and GB14922.2-2001. The laparotomy and extraction are carried out under sterile conditions, and two types of breeding mouse completely different from the carcass supply mice are adopted for breeding and purification, thereby achieving the purification effect with more advantages than the medical purification method, successfully obtaining the SPF reed vole for the first time, and laying a foundation for the establishment of an ideal animal model for schistosomiasis resistance, non-alcoholic fatty liver and other diseases and the research on pathogenic mechanisms of the diseases.

The invention relates to a technical filed of biological drugs and a multivalence recombinant protein subunit vaccine in chicken coccidiosis. The vaccine comprises four recombinant proteins in the chicken coccidiosis: TA4 (E.tenella), NA4 (E.necatrix), LDH (E.acervulina) and Em8 (E.maxima). It is proved through an animal immunity protective experiment that the vaccine can effectively resist four chicken coccidiosis infections and can be used in preparation of drug formulations for prevention against the chicken coccidiosis.

The invention relates to selection of a cucumber green mottle mosaic virus low virulent strain line and application of the cucumber green mottle mosaic virus low virulent strain line in cross protection. A wild type CGMMV-JN (GenBank No. KR232571) infectious clone is adopted as a basis, and mutation is introduced in fixed points in a genome of CGMMV through a site-directed mutation technique, so that four CGMMV mutants such as CP89A, CP114A, Rd68A, Rd869A and Rd1069A are obtained, wherein the pathogenicity of the mutants such as CP89A and Rd1069A is significantly reduced. The low virulent strain lines such as CP89A and Rd1069A can protect plants against being infected by CGMMV virulent strains.

The invention provides a biological safety enzyme-linked immunosorbent assay workstation and a method for performing enzyme-linked immuno sorbent assays by utilizing the same. The biological safety enzyme-linked immunosorbent assay workstation comprises a sample injecting device, a cleaning device, a warm bathing device, a colorimetric analyzing device and a biological safety protecting device. The sample injecting device comprises a three-dimensional-motion manipulator and an injection mechanism. The cleaning device comprises an injecting needle washing device, a plate cleaning device and cleaning buckets. The biological safety protecting device comprises a fan, an upper filtering mechanism and a lower filtering mechanism. The method for performing the enzyme-linked immuno sorbent assays by utilizing the biological safety enzyme-linked immunosorbent assay workstation includes that 1) reagent are sucked by the injecting needle; 2) samples or the reagent are distributed to cuvettes to perform reactions; 3) health statues of the assaying samples are determined by the colorimetric analyzing device according to absorbance values; 4) the cuvettes are cleaned by the plate cleaning device; 5) entire pipeline and the injecting needle is washed by cleaning fluid; 6) other samples or reagent are added; and 7) ultraviolet lamps are turned on to perform disinfection.

The invention belongs to the field of foods, and particularly discloses hypoallergenic infant formula goat milk powder. The hypoallergenic infant formula goat milk powder is prepared from raw goat milk, high-oil desalted goat whey powder, lactose, goat whey protein powder, soybean oil, 1, 3-dioleic acid-2-palmitoyl triglyceride, linseed oil, anhydrous butter, arachidonic acid powder, fish oil powder, composite mineral substances, decavitamin, lactoferrin, calcium carbonate, osteopontin, potassium hydroxide, choline chloride, nucleotide, phospholipid and lactobacillus paracasei N1115. The composition of the hypoallergenic infant formula goat milk powder is close to breast milk, nutrition for growth and development of infants is guaranteed, milk protein allergy of newborn infants is avoided,immunity of the newborn infants is enhanced, and therefore the newborn infants are prevented from being infected and getting sick. The hypoallergenic infant formula goat milk powder is suitable for being eaten by infants of 0-6 months old, and is particularly suitable for being eaten by newborn infants with low resistance.

The invention provides a microbial agent for preventing and treating apple ring rot and a preparation method thereof. The microbial agent is obtained by using a gel carrier loaded with compound microbial powder. The compound microbial agent comprises bacillus cereus ACCC03288 powder, trichoderma harzianum ACCC30371 powder, pseudomonas chlororaphis ACCC19853 powder, paecilomyces lilacinus ACCC30639powder and thorn spore streptomyces hygroscopicus ACCC40018 powder. The growth and reproduction of apple ring rot germs are synergistically inhibited, nutrition support is provided for apple trees, and the growth of apples is promoted. The gel carrier is obtained by utilizing a cross-linking reaction of acrylamide and sodium acrylate, cross-linking sodium alginate and calcium chloride and introducing polyvinyl alcohol, can be naturally degraded, has a slow release effect on the compound microbial powder, and provides nutrition support for the microbial growth, so that the compound microbial powder has a better control effect. The microbial agent only needs to be applied once, thereby reducing the labor intensity.

The present invention relates to an adenovirus vector vaccine for the prevention of an SARS-CoV-2 Ombke strain. According to the present invention, codon preference is adopted to optimize to obtain the new S gene sequence, and the new S gene sequence can be efficiently expressed in human cells, can efficiently express the S antigen after immunizing the body, can produce the neutralizing antibody aiming at the Ombucus chinensis strain SARS-CoV-2, and can effectively protect the body from the infection of the Ombucus chinensis strain.

The invention discloses a preparation method of a gel spray for wound repairing and the gel spray. The preparation method comprises the following steps of (1) dispersing locust bean gum into deionized water, adding sodium periodate, carrying out stirring reaction at 30-45 DEG C for 4-8h, transferring a reaction liquid to a dialysis bag of which the molecular cut off is 5,000 for dialysis to obtain oxidized locust bean gum; and (2) adding the oxidized locust bean gum obtained in the step (1) and polyoxyethylene diphenacylamine to a calcium chloride water solution, carrying out ultrasonic treatment for 10-30min and standing to obtain hydrogel for promoting healing of a wound. The hydrogel is capable of effectively promoting healing of the wound; meanwhile, the hydrogel is safe and free of thrill, has good biocompatibility and is used for wound treatment, and is capable of fully absorbing percolate of the wound due to good hygroscopic property and maintaining the wound surface physiologically moist; meanwhile, the hydrogel also has good antibacterial activity to avoid bacterial infection.

The invention provides a zizyphus maurtiana planting method and relates to the field of planting of zizyphus maurtiana. The zizyphus maurtiana planting method comprises the steps of field planting and water-fertilizer management, and further comprises the following step: spraying a certain amount of a microbicide in each growth period of zizyphus maurtiana. The microbicide comprises the following components: a bacillus subtilis agent, a trichoderma harzianum agent, a photosynthetic bacterium agent, a yeast agent, a bacillus thuringiensis agent and an enzyme enhancer preparation, wherein the enzyme enhancer preparation comprises such components as chitinase, glucanase and protease. By adoption of the zizyphus maurtiana planting method provided by the invention, the occurrence and spread of diseases and insect pests can be effectively controlled, the number of fruits with disease spots and the fruit dropping rate can be reduced; the quality and the yield of the zizyphus maurtiana can be improved; and the pollution-free planting effect can be achieved.

The invention relates to the field of strawberry planting and discloses a pollution-free strawberry planting method. The method comprises the following steps: (1) seedling culture: a seedling obtained through tissue culture and virus-free treatment is hardened and cultured, the seedling with good tolerance is screened layer by layer, and meanwhile, the infection of the seedling by viruses and pests is also avoided before transplanting, so that the survival rate is increased, and the infection rate of diseases and pests is reduced; (2)transplantation: the invasion of the strawberry seedling by diseases and pests hidden in the planting soil is mainly isolated, and meanwhile, a root protection material is used to prevent breeding of bacteria and pests on the strawberry leaf in a fertilizing process; and (3) maintenance: possible diseases of the strawberry are prevented and controlled by a plant disease and pest resistance preparation, and meanwhile, an artificial insect trap is used to solve the problems of fungal diseases and pests like red spiders and whiteflies bred quite easily in a greenhouse. The pollution-free strawberry planting method has the prominent advantages that no hormone pesticide is applied onto the strawberry, and the obtained strawberry is natural, pollution-free, large, solid, and high in sugar content.

The invention discloses an aviadenovirus Hexon and infectious bursal disease virus VP2 fusion antigen and subunit vaccine, and a preparation method and application of the aviadenovirus Hexon and chicken infectious bursal disease virus VP2 fusion antigen and subunit vaccine. The aviadenovirus Hexon and chicken infectious bursal disease virus VP2 fusion antigen comprises an aviadenovirus Hexon protein and an chicken infectious bursal disease virus VP2 protein. The fusion antigen has the following amino acid sequence: (1) a protein formed by an amino acid sequence as shown in SEQ ID No.1; or (2) an amino acid sequence of a functional protein with the same code and homology being 95 percent to 100 percent as the protein amino acid sequence limited by the sequence SEQ ID No.1. A protective antigen of the subunit vaccine prepared through the invention is the fusion antigen, and the fusion antigen is prepared by adopting a gene engineering fermentation method, and has the advantages of low cost, high antigen purity, good immunogenicity and high safety.

The invention provides a preparation method of an anti-neweastle disease virus specific transfer factor. The method comprises the following steps: selecting a healthy chicken as an experiment chicken to carry out supplementary immunization of a newcastle disease inactivated vaccine; monitoring the level of an immune antibody of the newcastle disease vaccine inside the experiment chicken body; taking splenic organs of the experiment chicken in a sterile condition when the level of the antibody achieves 28-29; and processing the splenic organs of the chicken, so as to obtain the anti-neweastle disease virus specific transfer factor. By adopting the anti-neweastle disease virus specific transfer factor disclosed by the invention, the specific immune effect of the chicken body on a neweastle disease virus antigen can be improved; normal cells of the body can be prevented from being infected by the virus. Thus, the transfer factor disclosed by the invention can play the roles in preventing the neweastle disease virus infection and protecting normal cells of the body from being damaged by the neweastle disease virus, so as to reduce the morbidity; meanwhile, the chicken infected by the neweastle disease virus can be treated by using the transfer factor. Therefore, the clinical symptoms can be effectively improved; the recovery rate is increased; the mortality is reduced.

The invention belongs to the technical field of veterinary biological products, and discloses a fused gene. A base sequence is as shown in a sequence table SEQ ID NO: 1. An avian influenza H9 subtypeHA gene, an adenovirus type 4 fiber2 gene and a specific connecting peptide segment gene are fused to form the fused gene. An expression protein antigen corresponding to the fused gene can induce poultry to generate high-level specific antibodies, and the poultry is protected from infection of avian influenza and avian adenoviruses. Besides, the invention further discloses a recombinant expressionvector, an antigen, a preparation method and an application.

The invention relates to a process for preparing anti-swine flu virus transfer factors. The transfer factors of the invention can play a role in preventing swine flu and protecting normal body cells from being invaded by swine flu viruses so as to reduce the morbidity. Meanwhile, the transfer factors for treating a swine suffering from swine flu virus infection can effectively improve the clinical symptoms, improve the cure rate and reduce the death rate. The process for preparing the transfer factors comprises the steps of preparing virus antigens, extracting the virus antigens, immunizing the swine by using the virus antigens, extracting the anti-swine flu virus transfer factors from the spleen of the immunized swine and the like.

The invention provides a brassica chinensis cultivation method with using wormcast produced from waste of a sugar refinery, and belongs to the technical field of vegetable plantation. The method comprises the steps that (1) a cultivation matrix is prepared by mixing bagasse, sugarcane filter mud, coal ash, wine slag, a calcium magnesium phosphate fertilizer, peanut bran and a fermented strain uniformly and stacking the same for fermentation, and adding an active nutrition agent, including cytex and thiamine hydrochloride, to wormcast 50-60 days after introduction of earthworm to obtain a cultivation matrix; (2) seeds are soaked and germination is accelerated; (3) sowing and seedling growing are carried out, plantation soil is sprayed with a weak trichoderma harzianum solution after sowing;and (4) thinning and field management is carried out by a foliar fertilizer is applied once in a six-leaf-one-center period of the brassica chinensis. According to the method, waste of the sugar refinery is changed into valuables, the waste is fermented and used for earthworm culturing, the wormcast is used for cultivation of brassica chinensis, the application amount of inorganic fertilizers forbrassica chinensis can be reduced, and the output and taste of brassica chinensis are ensured.

The invention relates to the technical field of influenza prevention and control, and provides a method, system and device for recommending a path based on an influenza risk, and a storage medium. Themethod comprises the following steps: detecting influenza symptom data in a target area, and acquiring risk users with influenza symptoms and risk-free users without influenza symptoms in the targetarea; generating a plurality of dynamic risk areas according to the risk users and the influenza symptoms thereof; calculating parameter values of advancing position points in the target area according to each dynamic risk area; and recommending a first path which can at least avoid the dynamic risk areas to the risk-free users and recommending a second path which can at least avoid the risk-freeusers to the risk users according to the parameter values of every advancing position point. According to the invention, the influenza risk areas are dynamically marked, a safe advancing path is guided, influenza risk diffusion in the target area is avoided, and the risk-free users are protected against infection.

The invention discloses an influenza new coronary combined vaccine. The influenza new coronary combined vaccine is prepared from the following raw materials in mass concentration: 1 to 100 micrograms / mL of recombinant new coronary vaccine containing RBD-Fc fusion protein, 1 to 50 micrograms / mL of influenza subunit vaccine containing H1N1 type influenza virus, 1 to 50 micrograms / mL of influenza subunit vaccine containing H3N2 type influenza virus, 1 to 50 micrograms / mL of influenza subunit vaccine containing B type influenza virus, an aluminum hydroxide solution and the balance of PBS phosphate buffer, wherein the final concentration of aluminum ions in the influenza new coronary combined vaccine is 0.5 to 2.0mg / mL. A preparation method comprises the following steps of: weighing all the raw materials; diluting the four vaccines with the PBS phosphate buffer respectively and then mixing with the aluminum hydroxide solution; and mixing according to an equal volume ratio to obtain the influenza new coronary combined vaccine. The influenza new coronary combined vaccine disclosed by the invention is a combined vaccine of a novel coronavirus vaccine containing an aluminum adjuvant and the influenza subunit vaccine containing the aluminum adjuvant; and after combination, the two antigen component vaccines are not mutually inhibited and can be very well compatible.

The invention relates to a sterilizing composite. The composite is characterized in that the effective ingredients of the composite contain ethirimol and at least one protective germicide B, wherein the germicide B can be sulfur, chlorothalonil, thiram, ziram, zineb, mancozeb, ambam, propineb or metiram. The result of indoor tests shows that the synergism of the composite to the powdery mildews is obvious, the cotoxicity coefficient is more than 130; and in the field actual application, the effect for controlling the resistant powdery mildews is obviously better than a single agent, no phytotoxicity is caused by the composite to crops, especially melons, and the pesticide residue is less, thus the composite is a practical and safe sterilizing composite for controlling the powdery mildews.

The invention discloses formula powder suitable for females at the pregnancy period and a preparation method of the formula powder, and belongs to the technical field of concoction milk powder and a nutrient food. The formula powder consists of lactoferrin, casein peptide phosphate, folic acid, ferrous gluconate, whey protein powder, galactooligosaccharide, fructo-oligosaccharide, docosahexaenoicacid, choline, taurine, vitamin A, vitamin D, vitamin C, vitamin E, vitamin K, vitamin B1, vitamin B2, vitamin B6, vitamin B12, niacin, biotin, calcium, magnesium, phosphorus, sodium, potassium, zinc,selenium and fresh milk spray-dried powder. The formula powder is suitable for females at the pregnancy period, various nutrient substances can well promote absorption with each other and provide nutrients necessary for females at the pregnancy period without additional nutrient enrichment, and the formula powder can enrich the calcium, can resist anemia, can improve the immune function and disease resistance of the females at the pregnancy period, can make early-stage preparation for the females at the pregnancy period, and finally, can realize the purpose of prenatal and postnatal care.

The invention discloses an chimeric antigen receptor gene for HIV-1. The gene comprises a guide subregion, a structural domain, a hinge region, a trans-membrane structural domain, a co-stimulation structural domain, a signal transduction structural domain and a suicide gene system region, wherein the structural domain is combined with a CD4 binding site of a gp120. The invention further disclosesa plasmid with the gene, a T cell, a kit and application. According to the chimeric antigen receptor gene for the HIV-1, the plasmid with the gene, the T cell, the kit and the application, when the Tcell is applied, HIV-1 infection can be prevented, and meanwhile, the lethality on an HIV-1 infected cell can be improved; and once an HIV-1-CAR-T cell is input, adverse reactions occur, the cell in abody can be cleared at once through a suicide gene system, and therefore the preventive effect can be achieved.