94results about How to "Not pathogenic" patented technology

The invention relates to an Asia1 type foot-and-mouth disease recombinant virus without pathogenicity for a host and a preparation method and application thereof. A saving system is efficient eukaryotic plasmids which are constructed by gene engineering and can express exact foot-and-mouth disease virus genome RNA (Ribonucleic Acid), and therefore the foot-and-mouth disease recombinant virus can be constructed and prepared; vaccine strains with high titer and good antigen matching property can be prepared by using the plasmids, can be prepared into live vaccines or inactivated vaccines and can effectively stimulate bodies to produce immune response after being used for immunizing pigs and cattle, provide an immune protective effect on the pigs and the cattle and effectively protect GV and GII prevalent strains, the immune protection rate can reach 100 percent, and the median protective dose (PD50) is 6.34 to 13.59; and the recombinant virus has the advantages of high titer, high antigen matching property with the prevalent strains, wide antigen spectrum and high immune protection rate, does not have pathogenicity for pig and cattle hosts, does not form toxemia or expel toxin, and can be applied to prevention and control of Asia1 type foot-and-mouth disease viruses of China and neighboring countries.

The invention discloses a virus (PRRSV) recombinant adenovirus rAd-GF35 of porcine reproduction and respiratory syndrome, which belongs to the technical field of high-tech biotechnology. Highly pathogenic PRRSV SY0608 separation strains of GP3 and GP5 protein genes and porcine colony sell stimulating factor (GMCSF) whole gene sequences are amplified, connected in series and cloned into pShuttle-CMV to be transformed into escherichia coli BJ5183 together with pAdEasy-1 so as to obtain recombinant plasmids; and HEK293A cells are transfected to obtain the recombinant adenovirus so as to successfully express PRRSV SY0608 strains GP3 and GP5 and a GMCSF protein. The recombinant virus can express the GMCSF correctly, and play a role of an adjuvant so as to improve the immune activity of the PRRSV GP3 and GP5 proteins and stimulate the immune protective reaction of an organism more effectively. The recombinant adenovirus vaccine has the safety of a subunit vaccine and the antigen proliferating ability of an attenuated vaccine, thereby having wide development and application prospect.

The invention discloses an immunization composition, comprising foot-and-mouth disease virus O structural proteins VP3 and VP1, and foot-and-mouth disease virus O structural proteins VP2 and / or VP4. Further, the immunization composition also comprises a foot-and-mouth disease virus O structural protein VP0. The immunization composition is applicable to the preparation of a novel genetically-engineered subunit vaccine to foot-and-mouth disease virus O; the novel genetically-engineered subunit vaccine is similar to natural proteins in terms of antigenicity, immunogenicity and functionality, the expression level is high, the immunogenicity is high, and the novel genetically-engineered subunit vaccine is non-pathogenic to an animal. In addition, the novel genetically-engineered subunit vaccine is suitable for large-scale serum-free suspension culture through biosensors; vaccine production cost is reduced greatly.

The invention provides an immunological composition and a subunit vaccine. The immunological composition comprises a protein which is selected from one or an arbitrary combination of two or more of mycoplasma gallisepticum-associated proteins encoded with nucleic acid molecules of SEQ ID NO: 1 or 3 or 5 or 7 or 9 or nucleic acid molecules which are 95% or above identical to the nucleotide sequenceof SEQ ID NO: 1 or 3 or 5 or 7 or 9. The vaccine adopts eukaryotic expression, the antigenicity and immunogenicity of the product are similar to those of a natural protein, the expression level is high, the immunogenicity is strong, the protective effect is good, and the vaccine has no pathogenicity to chickens; besides, large-scale serum-free suspension culture preparation of the vaccine can berealized through a bioreactor, and meanwhile the vaccine production cost is greatly reduced.

The invention discloses a method for genetic targeting of stem spermatogonium of poultry. By using an adenovirus of poultry as a targeting vector, the method performs targeting operation on the stem spermatogonium of the poultry; the obtained genetically modified stem spermatogonium can be used for transplanting testis and obtaining a transgenetic gene; and by mating the normal poultry, the stem spermatogonium can be further used for breeding the transgenetic poultry. The method has the advantages that: the method can effectively realize the high-efficiency genetic targeting of the stem spermatogonium of the poultry; the used homologous arm is short, and the vector is easily established; and after the exogenous objective albumen is directly integrated in an albumin expression albumen high-efficiency promotor, the expressional specificity of the exogenous albumen of the transgenetic offspring is high, the yield of albumen is high. Moreover, the method also has the advantages of simple operation, safety, no pathogenicity, low immunogenicity and less cell toxins.