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Two endophytic fungi strains having control effect on soft rot disease of dendrobium plants

An endophytic fungus and soft rot technology, applied in the field of bioengineering, can solve problems such as lack of standardized operation technology, environmental pollution, and failure to harvest cultivated crops.

Inactive Publication Date: 2011-11-09
INST OF MEDICINAL PLANT DEV CHINESE ACADEMY OF MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

When the disease is serious, soft rot can cause large-scale failure of cultivated crops, which seriously restricts the development of Dendrobium industry
At present, there are mainly two ways to prevent and treat the soft rot of Dendrobium plants: one is through cultivation management, such as controlling the humidity of the seedbed, ventilation, disinfection of water sources, and sunlight exposure treatment of the cultivation substrate, etc., which can play a role in the prevention and control of the disease. However, this kind of method is labor-intensive and time-consuming, the operation technology is not standardized, the controllability is poor, and the efficiency of disease control is not high; However, it is easy to cause drug resistance of pathogenic bacteria, and the extensive use of pesticides has a great effect on environmental pollution and ecological balance damage

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] Biocontrol of Dendrobium nobile soft rot by Dhs96

[0014] The endophytic fungus Dhs96 was cultured by PDA liquid fermentation and mixed evenly with wheat bran solid matrix to make solid inoculant. The spores of Dendrobium soft rot pathogen Pythium ultimum (Pythium ultimum) were collected after activation and cultured, and prepared into 5×10 5 / ml of spore suspension, mixed evenly with wheat bran solid matrix to make solid inoculant. The bark is used as the cultivation substrate, and the Dhs96 solid inoculant is inoculated into the roots of the Dendrobium nobile seedlings when the Dendrobium nobile seedlings are planted. The potted seedlings were placed in a solar greenhouse, and the temperature was maintained at 20±2°C. Spray water after planting to facilitate the growth of endophytic fungi. After that, spray water every three days. The solid inoculants of pathogenic bacteria were sprinkled around the Dendrobium nobile seedlings 3 days and 30 days after inoculating...

Embodiment 2

[0016] Biological control of soft rot in Dendrobium officinale seedlings by Dhs96

[0017] The endophytic fungus Dhs96 was cultured by PDA liquid fermentation and mixed evenly with wheat bran solid matrix to make solid inoculant. The spores of Dendrobium soft rot pathogen Pythium ultimum (Pythium ultimum) were activated and cultured to prepare 1×10 5 / ml of spore suspension, mixed evenly with wheat bran solid matrix to make solid inoculant. The bark is used as the cultivation substrate, and the Dhs96 solid inoculant is inoculated into the roots of the Dendrobium officinale seedlings when the Dendrobium officinale seedlings are planted. The potted seedlings were placed in a solar greenhouse, and the temperature was maintained at 20±2°C. Spray water after planting to facilitate the growth of endophytic fungi. After that, spray water every three days. The solid inoculants of pathogenic bacteria were sprinkled around the Dendrobium officinale seedlings 3 days and 30 days after...

Embodiment 3

[0019] Biological control of Dhs96 on soft rot of Dendrobium chrysanthemum seedlings

[0020] The endophytic fungus Dhs96 was cultured by PDA liquid fermentation and mixed evenly with wheat bran solid matrix to make solid inoculant. The spores of Dendrobium soft rot pathogen Pythium ultimum (Pythium ultimum) were collected after activation and cultured, and prepared into 5×10 4 / ml of spore suspension, mixed evenly with wheat bran solid matrix to make solid inoculant. The bark is used as the cultivation substrate, and the Dhs96 solid inoculant is inoculated to the roots of the Dendrobium chrysotox seedlings when planting the Dendrobium chrysotox seedlings. The potted seedlings were placed in a solar greenhouse, and the temperature was maintained at 20±2°C. Spray water after planting to facilitate the growth of endophytic fungi. After that, spray water every three days. The solid inoculants of pathogenic bacteria were sprinkled around the Dendrobium drumstick seedlings 3 da...

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Abstract

The invention discloses two endophytic fungi strains which have biological control effect on soft rot disease of orchid family dendrobium plants. The endophytic fungi strains can be colonized at a root of a dendrobium plant and used for seedling growing or cultivation of the dendrobium plant to effectively control soft rot disease of the dendrobium plant.

Description

technical field [0001] The invention relates to the technical field of bioengineering, in particular to two endophytic fungal strains with biological control effects on soft rot of Dendrobium plants. Using the two strains of fungi in the seedling raising or cultivation of the Dendrobium can effectively prevent the soft rot of the Dendrobium. Background technique [0002] There are many traditional and precious Chinese medicinal materials in Orchidaceae, such as Dendrobium officinale and Dendrobium nobile. According to the 2005 edition of "Chinese Pharmacopoeia", Dendrobium nobile Lindl., Dendrobium nobile Lindl. The fresh or dried stems of the species can be used as a traditional Chinese medicine Dendrobium, which has the effects of nourishing the stomach and promoting body fluid, nourishing yin and clearing heat, and can also relieve throat and improve eyesight, and regulate human immune function. [0003] Since the beginning of the 21st century, especially with the adven...

Claims

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Application Information

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IPC IPC(8): C12N1/14A01N63/04A01P3/00C12R1/845C12R1/885
Inventor 郭顺星李向东陈晓梅王卉
Owner INST OF MEDICINAL PLANT DEV CHINESE ACADEMY OF MEDICAL SCI
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