51results about How to "Reduce virus content" patented technology

The invention belongs to the technical field of animal virology and immunology, in particular to a double-antibody sandwich ELISA (Enzyme-Linked Immuno Sorbent Assay) kit for detecting Japanese encephalitis virus antigens in swine, human and mosquitoes. Core reagents of the kit comprise a Japanese encephalitis virus E protein monoclonal antibody serving as a primary antibody and a rabbit polyclonal antibody serving as a secondary antibody. The invention discloses a preparation and purification method of the Japanese encephalitis virus E protein monoclonal antibody and the rabbit polyclonal antibody. The invention also discloses a detection method of the double-antibody sandwich ELISA. A hybridoma cell strain secreting the monoclonal antibody is preserved in China Center for Type Culture Collection with CCTCC NO. C2010114.

The invention belongs to the field of biotechnology, and discloses a preparation method and application of specific antibody containing milk or serum produced by novel coronavirus immunized cows. Theinactivated novel coronavirus hCov-19 / Wuhan / ZY38-1 / 2020 is used for immunizing dairy cows, and a large amount of immune milk and serum can be obtained. Both the immune milk and the serum contain highcontent of antibodies, viruses can be effectively neutralized and stopped from invading cells, and the significant anti-SARS-CoV-2 virus effect is achieved; after Igs protein purified from immune milkis injected into mice, the mortality of novel coronavirus infected mice can be significantly reduced, the influence of novel coronavirus infection on weight loss of the mice is reduced, and the content of viruses in lung tissue of the mice is reduced. The immunized milk and serum can be used as sources of antibodies for preparations for preventing and treating novel coronavirus pneumonia, and thelimitation of the sources of plasma for rehabilitation of novel coronavirus pneumonia.

The invention relates to a heat treatment detoxification method for sweet potatoes. The heat treatment detoxification method specifically comprises the following steps: (1) preferably selecting sweet potato seedlings; (2) pretreating the sweet potato seedlings; and (3) detoxifying the sweet potato seedlings. Proved by experiments, detoxified sweet potato seedlings obtained by using the heat treatment detoxification method for sweet potatoes, provided by the invention, are better in detoxification effect, and the yield per unit area reaches up to more than 2940kg, while the yield per unit area of the planted detoxified seedlings obtained by carrying out stem tip tissue culture on detoxified stem tips obtained by using the existing heat treatment detoxification method for sweet potatoes by using the same method is only 2258kg; and meanwhile, the method disclosed by the invention is shorter in time consumption and better in detoxification effect; and the existing heat treatment detoxification method often takes 2-4 weeks or even longer, while the method disclosed by the invention only takes about 10 days.

The invention provides a system and method for exhausted air sterilization and aeration and comprehensive heating disinfection of a highly-contaminated building space. The system comprises a contaminated area negative pressure metal ventilation pipe, any position thereon communicates with an inlet end of a high-resistance thermal-insulation-layer-carried ventilation pipeline, an outlet end of thehigh-resistance thermal-insulation-layer-carried ventilation pipeline is connected to an inlet end of an outdoor negative pressure metal ventilation pipe through an electric anti-backflow valve, and an outlet end of the outdoor negative pressure metal ventilation pipe is connected with an exhaust port of a high-efficiency variable-frequency fan. Various virus killing devices are sequentially disposed at the rear end of the high-efficiency variable-frequency fan. The system disinfects the air that may contain viruses in the highly-contaminated building space at high temperature through an electric heating pipeline and then guides the air that is disinfected at high temperature to the various outdoor virus killing devices through negative pressure pipelines to diversely and thoroughly disinfect the guided air, thereby thoroughly removing viruses in the air in the highly-contaminated building space.

The invention discloses a prescription, which contains aqueous extracts of shiitake mushrooms, poria cocos, zingiber officinale and dried tangerine peels and plays a role in resisting H1N1 viruses, and application thereof. The prescription is mainly made up of the shiitake mushrooms, the poria cocos, the zingiber officinale and the dried tangerine peels. Proven by antiviral tests, the prescription disclosed by the invention can be used for remarkably improving the immunologic function of H1N1 virus infected mice and improving the pneumonia pathological change degree of the H1N1 virus infected mice and can be used for remarkably improving the specific antibody level in blood serum of the H1N1 virus infected mice, remarkably lowering the content of viruses in lungs of the H1N1 virus infected mice and improving the level of lung cell factors IL-2, so that the prescription has a good H1N1 influenza virus resisting action.

The invention provides an exhaust aeration disinfection and sterilization system and method for a high-pollution building space. The system involves a polluted area negative pressure metal ventilationpipe, wherein any position of the polluted area negative pressure metal ventilation pipe communicates with the inlet end of a high-resistance ventilation pipeline with a thermal isolation and insulation layer, and the outlet end of the high-resistance ventilation pipeline with the thermal isolation and insulation layer is connected to the inlet end of an outdoor negative pressure metal ventilation pipe through an electric backflow prevention valve; and the outlet end of the outdoor negative pressure metal ventilation pipe is connected to an extraction opening of an efficient variable-frequency fan, and a plurality of virus disinfection and sterilization devices are sequentially arranged at the rear end of the efficient variable-frequency fan. According to the exhaust aeration disinfectionand sterilization system and method for the high-pollution building space, air possibly containing viruses in the high-pollution building space is subjected to high-temperature disinfection and sterilization through an electric heating pipeline firstly, then the air subjected to high-temperature disinfection and sterilization is guided to the outdoor through a negative pressure pipeline, and theplurality of virus disinfection and sterilization devices carry out diversified thorough disinfection and sterilization on the introduced air so that the viruses in the air in the high-pollution building space can be thoroughly removed.

The invention discloses a planting method for saffron crocus tissue culture micro bulbotubers, and technical service is provided for seedling hardening planting in the later period of saffron crocus plant tissue culture. The planting method for saffron crocus tissue culture micro bulbotubers includes the following steps of seed source preparing, wherein seed selecting and sterilizing are involved; soil preparation, wherein the substeps of planting in a field, applying base fertilizer, disinfecting soil and carrying out furrow opening and ridging are included; planting and observing, wherein two substeps of sowing and observing are included. As the bulbotubers and soil are sterilized and disinfected by adopting multiple disinfecting methods, damage to the tissue culture micro bulbotubers by microorganisms is eliminated or reduced, and the planting survival rate is large; the method of two-time deep ploughing is adopted, and the soil is loosened, which is more beneficial for growth of saffron crocus. The planting method is easy to operate and low in cost, and large-scale industrial production is easier to achieve.

The invention belongs to the technical field of biology and discloses a novel coronavirus, a preparation method for specific-antibody-containing milk or serum produced by a dairy cow immunized by the novel coronavirus and application of the specific-antibody-containing milk or serum. A large volume of immunized milk and serum can be obtained through immunizing the dairy cow by using an inactivated novel coronavirus SARS-CoV-2 / ZY38-1, both the immunized milk and the serum contain high-content antibodies, viruses can be effectively neutralized, the viruses are prevented from invading cells, and the immunized milk and serum have an obvious SARS-CoV-2 virus resisting effect; and after an Igs protein purified from the immunized milk is injected into a mouse, the death rate of the mouse infected by the new coronavirus can be remarkably reduced, the influence of new coronavirus infection on the weight loss of the mouse is reduced, and the content of viruses in lung tissue of the mouse is lowered. The immunized milk and serum provided by the invention can be used as an antibody source of preparations for preventing and treating novel-coronavirus pneumonia, and the limitation of sources of rehabilitation blood plasma for the novel-coronavirus pneumonia is made up.

The invention relates to the technical field of sewage treatment, aims to the problem that municipal wastewater is hard to recycle or use after common physical filtering, and provides a municipal wastewater treatment method. According to the technical scheme, the method comprises the following steps: S1, performing primary filtering so as to obtain filtered water; S2, adding a flocculant into thefiltered water so as to obtain a flocculant mixed liquid; S3, heating the flocculant mixed liquid till boiling; S4, performing ultraviolet radiation, and performing sterilization and disinfection through vapor; S5, performing condensation; and S6, performing secondary filtering, wherein the flocculant comprises 100 parts of polyacrylamide, 20-25 parts of methylcellulose, 5-10 parts of casein and 0.01-0.02 part of 2-amino isonicotinic acid. The flocculant mixed liquid is heated till boiling to kill a part of bacteria, the vapor is radiated through an ultraviolet lamp, then a good permeation effect is achieved, a very good ultraviolet sterilization and disinfection effect can be achieved, the content of bacterial viruses in final pure water can be greatly reduced, a good sewage treatment effect can be achieved, the treated pure water can be recycled, waste source waste can be reduced, and meanwhile, pollution to the environment and water resources can be reduced.

The invention relates to a super-sensitivity method, primer group and kit for capturing whole genome of novel coronavirus. The method can conveniently and quickly amplify the whole genome of the novelcoronavirus by using a small amount of RNA, and can directly dock with a second-generation sequencing library-building reagent and a third-generation sequencing platform so as to obtain a whole genome sequence of the novel coronavirus.

The invention relates to micromolecular polypeptide capable of specifically inhibiting swine fever virus proliferation and application thereof and provides structure of the micromolecular polypeptide.The micromolecular polypeptide has the advantages that the micromolecular polypeptide is low in synthesis cost, convenient safe to use and capable of effectively inhibiting proliferation of swine fever virus and can be used with vaccines, thereby greatly improving vaccine antibody valence, prolonging time of antigen in vivo and prolonging immune protection period of vaccines.

The invention discloses a temporary emergency hospital ventilation system and method, including a negative pressure ventilation system and a ventilation protection system for medical staff; the negative pressure ventilation system includes a first air supply pipe connecting the medical corridor and the ward area; the ventilation protection system for the medical staff is arranged in the ward In the area and communicated with the outlet end of the first air supply pipe, it is used to track and locate the range or coordinates of the position of the human body in the ward area, and guide the air outlet direction of the first air supply pipe to point to the range of the position of the human body or coordinates. The beneficial effects of the temporary emergency hospital ventilation system of the present invention are: by setting a ventilation protection system for medical personnel at the outlet end of the first air supply pipe of the negative pressure ventilation system; the medical personnel in the ward area and the surrounding air are always affected by the flowing air flow , can effectively reduce the virus content in the surrounding air, thereby effectively protecting the safety of medical staff in the ward.

The invention relates to a medicine for preventing and treating the epidemic hemorrhagic fever. The medicine comprises the active pharmaceutical ingredients of ribavirin, prednisone and enalapril, and the three active pharmaceutical ingredients produce a synergetic effect. The medicine effectively kills the virus, the attach time of the symptom is remarkably shortened, the crossing stage rate is increased, the occurrence rate of the complication is reduced, and the treatment needs of a patient are better met.

The invention belongs to the field of biological preparations, and in particular relates to the application of 3-indoleacetonitrile in the preparation of drugs for inhibiting novel coronavirus SARS-CoV-2. The present invention finds for the first time that 3-indoleacetonitrile can inhibit the new coronavirus at the cell and animal levels, and can relieve the body's adverse symptoms caused by the new coronavirus infection. 2. It is effective in the infection model, which can significantly reduce the virus content in the lung tissue of mice, reduce the degree of weight loss of mice, and improve the survival rate of mice, which makes 3-indoleacetonitrile have great potential for clinical treatment.