76results about How to "Low homology" patented technology

The present invention provides recombinant lentiviral vectors and gene transfer systems which produce said vectors, cell lines utilized in the production of said recombinant lentiviral vectors, and Bovine Immunodeficiency Virus DNA sequences utilized in the recombinant vectors and gene transfer systems.

The invention discloses a multiple RT-PCR primer group for simultaneously detecting porcine gatahvirus (GETV), porcine epidemic diarrhea virus (PEDV), porcine deltacoronavirus (PDCoV) and porcine A rotaviruses (PoRV), which has a nucleotide sequence as shown in SEQ ID NO:1-SEQ ID NO:10. The invention further discloses a multiple RT-PCR detection method for detecting GETV, PEDV, TGEV, PDCoV and PoRV from a sample in one time by utilizing the multiple RT-PCR primer group. Compared with an existing conventional RT-PCR, the detection method has strong specificity and high sensitivity, can realizesimultaneous identification of five viruses including GETV, PEDV, TGEV, PDCoV and PoRV, and has accurate detection result and high detection efficiency.

The invention discloses a multiplex fluorescence PCR (polymerase chain reaction) universal adapter for microsatellite detection, which comprises an adapter 1, an adapter 2, an adapter 3 and an adapter 4, wherein the nucleotide sequences of the adapters 1-4 are disclosed as SEQ ID:1-4. The universal adapter has the advantages of high PCR amplification efficiency and no interference, and can not interfere with microsatellite primers to be amplified. The invention also discloses a method for carrying out microsatellite multiplex fluorescence PCR detection by using the multiplex fluorescence PCR universal adapter for microsatellite detection. In the multiplex PCR process, primer screening and typing experiments can be performed only after the four or three fluorescence adapters are synthesized. The method has the advantages of simple steps, short experimental period and low experimental cost.

The invention discloses a multiplex PCR (polymerase chain reaction) detection reagent of porcine circovirus type II, porcine parvovirus and porcine pseudorabies virus and special primers thereof. The invention provides the special primers for detecting the virus, and the special primers comprise a primer pair A, a primer pair B and a primer pair C, wherein the primer pair A comprises a primer 1 and a primer 2, the primer pair B comprises a primer 3 and a primer 4, the primer pair C comprises a primer 5 and a primer 6, and the nucleotide sequences of the primer 1, the primer 2, the primer 3, the primer 4, the primer 5 and the primer 6 are respectively as shown in the sequence 1, the sequence 2, the sequence 3, the sequence 4, the sequence 5 and the sequence 6 in a sequence table. Experiments prove that the multiplex PCR detection method of the porcine circovirus type II, the porcine parvovirus and the porcine pseudorabies virus provided by the invention is simple, convenient, fast, economical and efficient and has great application prospects in clinical diagnosis.

The present invention relates generally to constructs and their use in gene expression or gene regulation assays. More particularly, the present invention provides expression vectors and / or reporter vectors providing kinetics of protein expression with improved temporal correlation to promoter activity. Even more particularly, the invention provides expression vectors comprising a transcribable polynucleotide which comprises a sequence of nucleotides encoding a RNA element that modulates the stability of a transcript corresponding to the transcribable polynucleotide. The present invention provides, inter alia, novel vectors, useful for identifying and analysing cis- and trans-acting regulatory sequences / factors as well as vectors and genetically modified cell lines or organisms that are particularly useful for drug screening and drug discovery.

The invention discloses a stress-resistant gene SiRLK35 of setaria italica L.Beauv. as well as encoding protein and an application. Receptor-like protein kinase SiRLK35 is separated from setaria italica L.Beauv., the nucleotide sequence of the receptor-like protein kinase SiRLK35is shown in SEQ ID NO.1, and the gene encoding protein product belongs to serine / threonine protein kinase. The expression pattern of the gene in a stress condition is researched, the prokaryotic expression vector of the gene is established, the gene can be expressed in the protein level as is verified, the functions of the gene are primarily verified with a dot assay through a gene prokaryotic expression system, meanwhile, the growth curve of the gene under a salt treatment condition is detected, the gene is heterologously transformed into rice, a corresponding transgenetic plant is obtained, the functions of the transgenetic plant in salt resistance and stress resistance are identified primarily, targeted quality and character improvement on setaria italica L.Beauv. is further performed purposefully, the new stress-resistant germplasm of setaria italica L.Beauv. is innovated, and a guidance function is performed.

The present invention relates to immunogenic peptides, including variants and analogs derived from Streptococcus pneumoniae (S. pneumoniae) proteins, to peptide-multimers, conjugates and fusion proteins that include such peptides, and to vaccines that include such immunogenic entities. In particular, the present invention relates to the use of such vaccines for eliciting protective immunity to S. pneumoniae.

The present invention relates to compositions and methods for creation of vector nucleic acid sequences (e.g., retroviral nucleic acid sequences) that comprise two or more exogenous nucleic acid sequences that encode highly homologous (e.g., identical) polypeptide sequences, yet wherein at least one of the exogenous nucleic acid sequences has been mutated using degenerate codons for purpose of reducing homology between the two or more exogenous nucleic acid sequences while maintaining the encoded polypeptide sequence. Preferred nucleic acid sequences include those encoding multi-chimeric immune receptor (CIR) genes. Specific nucleic acid sequences of such CIR genes are also disclosed.

The present invention discloses a specific primer sequence of cattle Y-chromosome and its application in cattle embryo six identification. Cattle ear tissue DNA is first extracted to perform primer specificity identification; and embryo cell sample DNA is then extracted to perform PCR sex identification. The bull sample may be amplified to 255 bp segment when external primer is used in PCR reaction and to 205 bp when internal primer is used, while cow sample has no any amplified product, and this shows the bull specificity of the primer and the applicability of the primer in identifying cattle embryo sex. The present invnetion has the advantages of less pollution, high accuracy and high sensitivity and is suitable for field operation.

The invention discloses dual RT-PCR (reverse transcription-polymerase chain reaction) detection for a CDV (canine distemper virus) and a CPIV (canine parainfluenza virus) and a special primer for the detection. The invention provides the special primer for virus detection. The primer comprises a primer pair A and a primer pair B, wherein the primer pair A comprises a primer 1 and a primer 2; the primer pair B comprises a primer 3 and a primer 4; and nucleotide sequences of the primer 1, primer 2, primer 3 and the primer 4 are sequence 1, sequence 2, sequence 3 and sequence 4 respectively in a sequence table. Experiments prove that the special primer for dual RT-PCR detection for the CDV and the CPIV has good sensibility, can at least detect the RNA (ribonucleic acid) content to be about 1*10<-3>ng / mu L, the sensitivity is far higher than that of 1 ng / mu L. A dual RT-PCR detection method is simple, convenient, fast, economical and efficient, and has good application prospect.

The present invention relates to a novel anti-HER2 antibody or antigen-binding fragment thereof used in the prevention or treatment of cancer, a chimeric antigen receptor comprising the same, and usesthereof. The antibody of the present invention is an antibody that specifically binds to HER2 which is highly expressed in cancer cells (particularly, breast cancer or gastric cancer cells), and binds to an epitope that is different from an epitope to which conventional trastuzumab binds. Compared to transtuzumab, the antibody of the present invention exhibits a better killing ability with respect to kill HER2-unexpressed cancer cells which have non reactivity (or resistance) to a trastuzumab antibody or have reduced sensitivity. In addition, when the anti-HER2 antibody of the present invention is administered in combination with trastuzumab, a synergistic killing ability with respect to a cancer cell line on which the trastuzumab antibody acts. Therefore, a composition of the present invention can be very usefully used for combined administration with a trastuzumab antibody for the treatment of cancer, and for the treatment of cancer not treated by trastuzumab.

The invention discloses a high-temperature-resistant cabbage type rape glyoxalase gene and protein as well as applications thereof, and relates to the technical field of mutation and genetic engineering. The nucleotide sequence of the high-temperature-resistant cabbage type rape glyoxalase gene namely a BnGLYI gene is shown by SEQ ID NO1; and the amino acid sequence of the protein of the high-temperature-resistant cabbage type rape glyoxalase gene namely the BnGLYI gene is shown by SEQ ID NO2. The invention lays an experimental foundation for boosting the resource development of good genes of cabbage type rapes, and improving the heat resistance and cold resistance of the cabbage type rapes; heat-resistant and cold-resistant gene resources are further expanded; the BnGLYI gene is converted into yeast cells to ensure that the heat resistance and cold resistance of the yeast cells are significantly improved, so that the heat resistance and cold resistance of other microorganisms and plants are expected to be further improved.

The invention relates to a pathogenic microorganism DNA detection chip and the chip comprises a carrier and a nucleic acid probe on the carrier. The nucleic acid probe is provided with a target detection probe used for detecting target gene of pathogenic microorganism. The pathogenic microorganism comprises but not limits to vibrio cholerae, pathogenic escherichia coli, campylobacter jejuni, Yersinia enterocolitica, parahemolytic vibrio, salmonella, and shigella and Listeria monocytogenes. The invention also relates to a preparation method of the pathogenic microorganism DNA detection chip andprovides applications of the pathogenic microorganism DNA detection chip in detection of pathogenic microorganism. Also a pathogenic microorganism DNA detection chip kit is provided.