Kit and method for detecting porcine parvovirus, porcine circovirus type 2 and mixed infection of porcine parvovirus and porcine circovirus type 2

A porcine circovirus and parvovirus technology, applied in the biological field, can solve problems such as mixed infection, economic loss in pig farming, and difficulty in distinguishing, and achieve good application prospects and the effect of large-scale detection

Inactive Publication Date: 2014-05-28
BEIJING UNIV OF AGRI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] Diseases caused by the above viruses exist in many pig farms, causing huge economic losses to the pig industry
Clinically, mixed infections often appear, mainly manifested as stillbirths, deformed fetuses, mummified fetuses and sick piglets in infected sows, which are not easy to distinguish

Method used

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  • Kit and method for detecting porcine parvovirus, porcine circovirus type 2 and mixed infection of porcine parvovirus and porcine circovirus type 2
  • Kit and method for detecting porcine parvovirus, porcine circovirus type 2 and mixed infection of porcine parvovirus and porcine circovirus type 2
  • Kit and method for detecting porcine parvovirus, porcine circovirus type 2 and mixed infection of porcine parvovirus and porcine circovirus type 2

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Embodiment 1

[0019] Example 1 Porcine parvovirus (PPV), porcine circovirus type 2 virus (PCV2), and double PCR detection methods were established.

[0020] 1. Primer design: search for multiple virus strains in GenBank, perform homology analysis on them, use Primer5 to design primers for the above-mentioned conserved regions, and perform primer-dimer analysis on the designed two virus primers to avoid primers. Form a stable primer-dimer between them, and conduct homology or complementarity analysis on the amplified sequence of each pair of primers to avoid high homology or complementarity between them, so as to determine the primer sequences of the two viruses , see Table 1.

[0021] Table 1

[0022]

[0023] 2. Optimization of PCR reaction conditions

[0024] Use the DNA extraction reagent of this kit to extract porcine parvovirus (PPV) and porcine circovirus type 2 (PCV2) genomic DNA respectively, and the extraction method is carried out according to the operating instructions of t...

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Abstract

The invention aims to provide nucleotide primers for detecting porcine parvovirus and porcine circovirus type 2, which respectively are PPVF, PPVR, PCV2F and PCV2R, wherein the sequences PPVF and PPVR are a sense primer and an antisense primer for detecting the porcine parvovirus; the sequences PCV2F and PCV2R are a sense primer and an antisense primer for detecting the porcine circovirus type 2. A porcine parvovirus and porcine circovirus type 2 double-PCR detection method provided by the invention can simultaneously detect two viruses of PPV and PCV2 by one-time reaction; two pairs of used primers have good specificity; homology and complementarity between the primers are low; the primers have good sensitivity. The method has the advantages of simplicity, convenience, rapidness, economy, high efficiency and the like, is beneficial for a great quantity of clinic detection and has a good application prospect in clinical diagnosis.

Description

technical field [0001] The invention provides a kit and method for detecting porcine parvovirus, porcine circovirus type 2 and mixed infection thereof, belonging to the field of biotechnology. technical background [0002] Porcine parvovirus (PPV) and porcine circovirus type 2 (PCV2) are common DNA viruses that cause reproductive disorders in pigs. Currently, [0003] The diseases caused by the above-mentioned viruses exist in many pig farms, causing huge economic losses to the pig industry. Clinically, mixed infections often appear, mainly manifested as stillbirths, deformed fetuses, mummified fetuses and sickly piglets in infected sows, which are not easy to distinguish. Contents of the invention [0004] An object of the present invention is to provide a nucleic acid for detecting porcine parvovirus and porcine circovirus type 2 virus [0005] The nucleotide primers are respectively PPVF, PPVR, PCV2F and PCV2R, wherein the sequences PPVF and PPVR are sense primers a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/70C12Q1/68C12R1/93
CPCC12Q1/686C12Q1/70C12Q2531/113C12Q2537/143
Inventor 张志刚董剑辉张文利张晓杰李爽
Owner BEIJING UNIV OF AGRI
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