Kit and method for detecting porcine parvovirus, porcine circovirus type 2 and mixed infection of porcine parvovirus and porcine circovirus type 2
A porcine circovirus and parvovirus technology, applied in the biological field, can solve problems such as mixed infection, economic loss in pig farming, and difficulty in distinguishing, and achieve good application prospects and the effect of large-scale detection
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[0019] Example 1 Porcine parvovirus (PPV), porcine circovirus type 2 virus (PCV2), and double PCR detection methods were established.
[0020] 1. Primer design: search for multiple virus strains in GenBank, perform homology analysis on them, use Primer5 to design primers for the above-mentioned conserved regions, and perform primer-dimer analysis on the designed two virus primers to avoid primers. Form a stable primer-dimer between them, and conduct homology or complementarity analysis on the amplified sequence of each pair of primers to avoid high homology or complementarity between them, so as to determine the primer sequences of the two viruses , see Table 1.
[0021] Table 1
[0022]
[0023] 2. Optimization of PCR reaction conditions
[0024] Use the DNA extraction reagent of this kit to extract porcine parvovirus (PPV) and porcine circovirus type 2 (PCV2) genomic DNA respectively, and the extraction method is carried out according to the operating instructions of t...
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