49results about How to "The test result is sensitive" patented technology

The invention belongs to the technical field of molecular biotechnologies and particularly discloses an application of an EB1 gene to detection of nosema bombycis. Researches prove that the transcriptional expression of the EB1 gene is related to the cell division of the nosema bombycis and the development of a host. A specific primer is designed by taking the EB1 gene as a target gene, whether silkworm eggs are infected by the nosema bombycis is detected through PCR (Polymerase Chain Reaction) amplification, and the detected result proves that if the EB1 gene is used as the detecting target gene, the interference effects of inhibiting substances in silkworm egg extracts to the effective PCR amplification of pathogenic gene DNA (Deoxyribose Nucleic Acid) are very low, the detected result is more accurate and sensitive, and most importantly, whether the silkworm eggs are infected by the nosema bombycis can be rapidly detected at the early stage that the silkworm eggs are infected. The guarantee is provided for the detection and safe egg production of poisonous silkworm eggs in silkworm egg production.

The invention provides bias voltage temperature instability detection circuit and detection method. The bias voltage temperature instability detection circuit comprises an odd number of fundamental oscillation units. Each fundamental oscillation unit comprises a first transistor, a second transistor, a first control transistor, a second control transistor, an input end and an output end. The detection circuit further comprises third transistors which are located between adjacent fundamental oscillation units. The fundamental oscillation units and the third transistors are connected in series to form an annular oscillator. According to the embodiment of the invention, the bias voltage temperature instability detection circuit can respectively detect the degree of threshold voltage degradation, which is caused by negative bias voltage temperature instability, of a PMOS transistor and the degree of threshold voltage degradation, which is caused by positive bias voltage temperature instability, of an NMOS transistor; by using the third transistors, the degree of threshold voltage degradation, which is caused by bias voltage temperature instability, of an MOS transistor can be amplified; and the final detection result is sensitive and the detection precision is high.

The invention belongs to the technical field of organic photoelectric functional materials, and particularly relates to an ionic type iridium complex with double phosphorescence emission properties as well as a preparation method and an application of the ionic type iridium complex. The complex comprises a metal center and cyclometalated ligands. The preparation method comprises steps as follows: dichloro bridge is prepared from a phenylpyridine derivative and iridium(III) chloride trihydrate through a coordination reaction, and the ionic type iridium complex with the double phosphorescence emission properties is prepared from dichloro bridge and a dipyridyl derivative or sodium carbonate through coordination reaction. The ionic type iridium complex with the double phosphorescence emission properties can be applied to the fields of biological detection and biological imaging for hypoxic oxygen-enriched conditions; a diagnosis and treatment integrated multifunctional probe based on the ionic type iridium complex with the double phosphorescence emission properties has great potential in further biomedicine application.

The invention discloses an automatic feeding device for washing additives and a washing machine. The device comprises a container for containing the washing additives and a mounting part for carryingthe container, wherein the container is arranged in the mounting part and capable of being picked and placed; the device also comprises at least one group of liquid level detection structures for detecting the liquid level in the container. The container is placed in the mounting part and capable of being picked and placed, so that the additives can be supplemented conveniently, and when the container is placed back in the mounting part, the at least one group of liquid level detection structure can continue to detect the liquid level sensitively and accurately.

The invention belongs to the technical field of photoelectric functional organic materials and in particular relates to a preparation method and application of a metallic phosphorescence complex. Themetallic phosphorescence complex comprises a metal center and a cyclic metallic complex. The preparation method comprises the following step: performing a complexing reaction on N-N ligand and a C-N ligand with an amino chain, thereby obtaining the metallic phosphorescence complex. Through variations of service lives, the content of oxygen in organelle is relatively effectively judged, and different types of organelle can be well separated by using an FLIM (Fluorescence-Lifetime Imaging Microscopy) technique, so that the metallic phosphorescence complex has great significance for oxygen detection in cells, cytomembrane and mitochondria can be effectively separated by using the FILM technique, and the invention further discloses application of the metallic phosphorescence complex in fieldssuch as biological imaging, cell marking and oxygen detection.

The invention relates to a transgenic cotton detection chip, which comprises a substrate and a nucleic acid probe positioned on the substrate, wherein the nucleic acid probe may be one or more of nucleic acid probes having the sequences SEQ ID No.3, SEQ ID No.5, SEQ ID No.8, SEQ ID No.11, SEQ ID No.14, SEQ ID No.17, SEQ ID No.20 and SEQ ID No.23. The invention also relates to a kit having the transgenic cotton detection chip, a method for detecting transgenic cotton and the use of the transgenic cotton detection chip and the kit in the detection of the transgenic cotton. The transgenic cottondetection chip can be used to detect the transgenic cotton simply, quickly, specifically and sensitively with high throughput.

The invention discloses a method for rapidly determining melamine in milk and dairy products by liquid chromatography-tandem mass spectrometry (LC-MS / MS). According to the invention, an internal standard is added to a dairy product requiring detection; melamine is extracted by using acetonitrile; a normal-phase chromatographic column is adopted; a solution of acetonitrile and an ammonium acetate buffer salt is adopted as a mobile phase, and melamine is subject to reversed-phase separation; with tandem mass spectrometry, melamine in the milk and dairy products is subject to MRM qualitative and quantitative analysis. With a national standard method, purification is carried out by using an SPE columella, and a final constant volume is 1mL. With the rapid method provided by the invention, a final volume is 25mL. The adopted curve linearity range is 0.25ng*mL<-1> to 5.00ng*mL<-1>. When the value is multiplied 25 times the dilution difference, the value is 6.25mg / kg to 125mg / kg, which reaches and satisfies the requirement of the national standard. With the method, the sensitivity is improved, and the possibility of an overloaded sample is reduced.

The invention relates to an internal quality control kit for detecting transfusion compatibility and a production process thereof. The kit comprises a body (1), a test tube stand (2), a sample tube I (3), a sample tube II (4), a sample tube III (5) and a sample tube IV (6), wherein the preparation process of the sample tube I (3) and the sample tube II (4) comprises filtration, washing, suspension, subpackage and labelling; and the preparation process of the sample tube III (5) and the sample tube IV (6) comprises thawing, filtration, liquid preparation, subpackage and labelling. The kit and the production process have the following beneficial effects: the quantity of sample tubes is small; and the kit is simple and convenient to operate, saves time, has sensitive detection results and is suitable for various detection methods.

The invention discloses a primer group, a kit and a method for detecting six mouse viruses by multiple immunity fluorescence. The primer group is designed by the aid of multiple RT-PCR (reverse transcription-polymerase chain reaction) technologies and Luminex liquid chip technologies and can simultaneously detect six high-infection viruses such as TMEV, MHV, MNV, Reo-3, MVM and MPV of mice. By the aid of the primer group, target amplification products are acquired through specific PCR, the amplification products, fluorescence encoding microspheres and streptavidin-phycoerythrin are hybridized, and different types of viruses are distinguished when a mean fluorescence intensity value is read by a Luminex detector. The method has the advantages of rapidness, high efficiency, high specificity and sensitivity, good repeatability and the like and can be applied to quality monitoring, epidemiological investigation and early warning of test mice. The primer group is good in flexibility, and varieties detecting the viruses can be increased and decreased as required on the basis.

The invention discloses an enhanced chemiluminiscence detection kit. The enhanced chemiluminiscence detection kit comprises a solution A and a solution B, wherein the solution A is 10-200 mM of a trihydroxymethyl aminomethane buffer salt solution comprising 0.1-10 mM of luminol, 1-10 mM of 3-(10'-phenothiazinyl)propane-1-sulfonate, and 0.5-3 mM of 4-morpholinyl pyridine; and the solution B is 1-10mM of a urea peroxide solution. A novel enhancer and an electron transfer intermediate are adopted, the proportion of the components is adjusted, the more effective peroxide is matched, so that a more sensitive detection result is acquired, a target protein is detected from the pic level to the femtogram level; relatively high light intensity is kept within one hour after the reaction starts, thethermal acceleration experiment is conducted at the temperature of 37 DEG C for 2 weeks, but the product effect is not obviously affected, and the enhanced chemiluminiscence detection kit can be stored stably at the temperature of 4 DEG C for 1 year.

The invention discloses a primer group for simultaneously detecting five pathogens of rats, a kit and a multiple immunofluorescence analysis method. A multiple PCR (polymerase chain reaction) technology and a Luminex liquid phase chip technology are combined; the primer group capable of simultaneously detecting the five pathogens including RTV (rat theilovirus), TMEV (Theiler's murine encephalomyelitis virus), RCV (rat coronavirus), PVM (pneumonia virus of mice) and M. pulmonis of rats is designed. The primer group uses the specific PCR to obtain a target amplification product; then, the amplification product, fluorescence coded microspheres and streptavidin-phycoerythrin are hybridized; different types of pathogens are distinguished by reading an MFI value through an Luminex detection instrument. The method has the beneficial effects of high speed, high efficiency, high specificity, high sensitivity, good repeatability and the like, and can be applied to the experiment rat quality monitoring, epidemiology survey and early-stage early warning. The primer group has the advantages that the flexibility is good; the pathogen detection types can be increased or decreased on the basis according to requirements.

The invention discloses a phosphorescent iridium complex and a preparation method and application thereof. The phosphorescent iridium complex is prepared from a metal center and ring metal ligands, and ester groups are modified in different positions. The preparation method comprises the following steps: taking 2-phenylquinoline and derivatives thereof as C^N ligands, taking bipyridyl derivativesor phenanthroline derivatives as N^N ligands, and performing coordination reaction to synthesize the phosphorescent iridium complex. The phosphorescent iridium complex can target the cell nucleus, haslight stability superior to commercial cell nucleus dye, and can perform imaging and detection on each stage in a cell cycle. The phosphorescent iridium complex can realize effective removal of short-life cell background fluorescence signals through a time resolution technology to reduce the interference of background signals to probe light signals, thereby obtaining more sensitive and reliable detection results. The phosphorescent iridium complex disclosed by the invention is a multifunctional probe integrating diagnosis and treatment, realizes accurate detection in complicated environments,and has great potential in future biomedical application.

The invention provides an SNP (single nucleotide polymorphism) typing detecting method for utilizing a locking-type probe for rolling ring amplification. At first, the locking-type probe and an amplification primer are designed, then the designed locking-type probe is phosphorylated, then a locking-type probe cyclization reaction is carried out, an enzyme digestion reaction is adopted for digesting an uncyclized locking-type probe and genomic DNA to obtain a cyclized locking-type probe, the cyclized locking-type probe is subjected to a rolling ring amplification reaction, a reactant obtained through the rolling ring amplification reaction is taken and subjected to 1% agarose gel electrophoresis detection, and the gene type of SNP can be judged through an electrophoresis detecting result. According to the method, SNP typing detection can be directly carried out on biological samples like peripheral blood, the genomic extraction process is omitted, the operation steps are greatly reduced, detecting time is greatly shortened, cost is saved, the risk of cross infection of operators is reduced, the detecting method has great popularization prospects, and the detecting result is accurate and sensitive.

The invention provides an indirectly-heated silicon-based film catalytic hydrogen sensor, which is obtained by an MEMS processing technology and comprises a silicon substrate. The upper surface of thesilicon substrate is provided with a heat insulation layer, and the lower surface of the silicon substrate is provided with one or a pair of heat insulation grooves extending to the heat insulation layer. A pair of heating coils made of high-resistance temperature coefficient materials is arranged on the surface of the heat insulation layer, catalytic film layers are arranged on the inner sides,the outer sides or the upper portions of the heating coils, a high-temperature-resistant medium layer covers one surface of each catalytic film layer, a gap is reserved in the other surface of each catalytic film layer, and precious metal film resistors are arranged on the outer ring of the heat insulation layer. The hydrogen sensor provided by the invention can work in a catalytic combustion modeand a thermal conductivity mode at the same time, and is small in size, low in power consumption, quick in response and long in service life.

The invention belongs to the technical field of tick detection, and particularly relates to a method and kit for detecting tick pathogen nucleic acid based on a suspension array. The method for detecting tick pathogen based on the suspension array comprises the following steps of S1, designing a PCR primer, a Tag probe sequence and a double-binding probe sequence, wherein the PCR primer is used for amplifying six target gene segments and one reference gene, and the Tag probe is a probe which is modified by AminolinkerC12 at the 5' tail end, is designed aiming at the six target genes and the reference gene and is used for marking the fluorescent microspheres. According to the method and the kit for detecting the tick pathogen nucleic acid based on the suspension array, high-flux multiple detection is performed on six common tick-borne pathogens by adopting a suspension array technology, and the method and the kit have the advantages of high detection speed, high sensitivity, complete pathogen coverage and the like, and can be used as a favorable diagnostic tool for tick detection and evaluation of human and animal infection.

The invention discloses a fast screening method of an anti-bacilus tuberculosis medicine. The method comprises the following steps: adding a PHBS solution containing a medicine to be screened and serum into a bacilus tuberculosis suspension; incubating the mixture in an incubator at 20-36 DEG C for 3-5 days, then adding a Nitrocefin solution and continuing the incubation for 20-40 minutes; and measuring the OD500 value, wherein when the OD500 value is less than 0.64, the medicine to be screened is the anti-bacilus tuberculosis medicine. The method is substantially based on an in-vitro screening model of bacilus tuberculosis cellular level with the best representativeness, and the detection result has strong representativeness; the method can test through an elisa plate, has the advantages of small sample dosage, short detection period and relatively sensitive detection result, and can realize high-flux screening; the screening period is short, the incubation time of the bacilus tuberculosis is shortened to about 4 days, and the screening cost is low; and meanwhile, the pollution is avoided, and the screening accuracy is high.

The invention discloses a DMD gene exon copy number variation detection method and application thereof and belongs to the technical field of molecular biology. 79 exons of the DMD gene are amplified in a PCR reaction solution, meanwhile, one or more than two groups of reference genes are used for monitoring the PCR reaction efficiency, PCR products are analyzed through capillary electrophoresis, calculation is carried out according to DMD female controls with the DMD genes being 2 copy numbers, relative quantitative detection is carried out on the copy numbers of the 79 exons of the DMD gene, the DMD genes with the copy numbers of 0, 1, 2 and 3 are calculated, normal exon copy number, deletion mutation and repeated mutation of the DMD gene are distinguished, and small deletion and mutation can be prompted. A result can be obtained within 4 hours from a DNA sample, the condition of the exon copy number of the DMD gene can be detected only through one-time PCR experiment, the detection technology is simple in process, and standardization is easy to achieve.

The invention relates to a method and a kit for detecting respiratory infectious disease viruses based on a liquid chip system. A Tag probe and a double-binding probe for detecting eight human respiratory infectious disease viruses are designed, the probes are respectively cross-linked and mixed with fluorescent coding microspheres with different colors to obtain the liquid chip for detecting theviruses, and the double-binding probe is combined with a target fragment to realize fluorescence detection, so that clinical virus detection and diagnosis time can be greatly shortened, diagnosis efficiency and diagnosis accuracy are improved, in addition, the flux is high, and the market potential is large.

The invention discloses a method for detecting patulin in apple juice. The method for detecting the patulin in the apple juice comprises the following steps that A, the concentrated apple juice is diluted; B, a liquid sample is obtained and is placed in a centrifugal pipe; C, an ethyl acetate / normal hexane extracting solution is added to the liquid sample, and then anhydrous sodium sulfate is added to the liquid sample; D, a cover of the centrifugal pipe is tightened, the centrifugal pipe is oscillated, then ultrasonic waves and vortexes are applied to the centrifugal pipe, and centrifugation is conducted; E, 2.5 ml of organic liquid is placed in the centrifugal pipe, and blowing is conducted for five to eight minutes by introducing nitrogen to the centrifugal pipe; F, water is added to the organic liquid added to the centrifugal pipe in the step E, and then ultrasonic waves are applied to the centrifugal pipe; G, the liquid sample obtained in the step F is placed in a sample bottle, an internal label solution is added to the sample bottle, and a sample is obtained after purification; H, a solution is extracted from the sample obtained in the step G, detection is conducted through a liquid chromatogram series mass spectrometer in the multi-reaction detection mode, and then detection is completed. According to the method, both time and labor are saved during detection, production cost is reduced, a detection result is more accurate, and detection sensitivity is higher.

The invention provides a reaction system for detecting an EML4-ALK fusion gene based on digital PCR and applications of the reaction system. A detection system comprises a specific primer and a probe of the EML4-ALK fusion gene. By screening and optimizing primer and probe sequences and concentration proportion combination in a PCR amplification system, the detection rate of fusion mutation is increased, to assist a special RNA sample pretreatment process, so as to further reduce false positive probability. In addition, a digital PCR platform is used. The detection rate of various types of samples is remarkably improved. The detection on trace samples, such as free nucleic acid and exosome RNA, shows higher sensitivity and detection success rate.

The invention belongs to the technical field of DNA methylation analysis, and discloses an electrochemical analysis method based on a tetrahedral DNA nanoprobe and application, and the electrochemical analysis method comprises a tetrahedral probe structure sequence, different DNA methylation target sequences, a signal amplification sequence and a comparison sequence. According to the present invention, the optimal performance is represented under the conditions of the tetrahedral probe fixation time of 3 h, the target sequence hybridization time of 1 h and the HpaII incision enzyme digestion time of 2 h, the good detection ability is represented within the range of 1 amol / L to 1 pmol / L, the detection limit reaches 0.93 amol / L, and the sensitivity is high; the electrochemical biosensing technology has the unique advantages in the aspect of DNA methylation detection: specific analysis on a methylated DNA target sequence can be completed within a short time, the operation is convenient, the price is low, the sensitivity is high, the specificity is good, the stability is strong, and the electrochemical biosensing technology can be effectively used for DNA methylation detection in complex systems such as serum.

The invention provides a push rod displacement monitoring mechanism of a vehicle clutch. The push rod displacement monitoring mechanism comprises a clutch and a push rod, wherein the push rod is slidably mounted on a shell of the clutch, and the push rod receives thrust transmitted by a clutch pedal to extrude a diaphragm spring of the clutch to deform. The push rod displacement monitoring mechanism is characterized by further comprising a rotating column and an encoder, wherein the rotating column is rotatably mounted on a bracket of a chassis, the rotating column is located on the side edgeof the push rod, the encoder for detecting the rotating angle of the rotating column is mounted on the rotating column, a connecting rod is mounted in the center of the top end of the rotating column,a follower is arranged at the other end of the connecting rod, an arc-shaped main limiting groove matched with the follower is formed in the push rod, the main limiting groove and the rotating columnare concentric, the follower is inserted into the main limiting groove, and when the push rod is translated, the follower of the connecting rod moves in an arc-shaped trajectory along the main limiting groove to drive the rotating column to rotate. The push rod displacement monitoring mechanism can solve the problems that an existing push rod displacement monitoring mechanism is not compact enough in structure and the detection precision needs to be improved.

The invention discloses a Chinese Holstein cow paternity testing method and the special primer and reagent box thereof. And the primer comprises the following primer pairs: 1) primer pair composed ofnucleotide sequences 1 and 2 in sequence list; 2) primer pair composed of nucleotide sequences 3 and 4; 3) primer pair composed of nucleotide sequences 5 and 6; 4) primer pair composed of nucleotide sequences 7 and 8; 5) primer pair composed of nucleotide sequences 9 and 10; 6) primer pair composed of nucleotide sequences 11 and 12; 7) primer pair composed of nucleotide sequences 13 and 14; 8) primer pair composed of nucleotide sequences 15 and 16; and 9) primer pair composed of nucleotide sequences 17 and 18.

The embodiment of the invention discloses a method for detecting intracellular cytochrome c based on near-infrared fluorescence lifetime imaging, which comprises the following steps: mixing a gold nanorod particle solution marked with a nucleic acid aptamer and a near-infrared organic fluorescence molecular solution marked with a nucleic acid aptamer, centrifuging, dispersing the obtained precipitate in a buffer solution, and drying to obtain the intracellular cytochrome c based on near-infrared fluorescence lifetime imaging. The nucleic acid aptamers marked on the near-infrared organic fluorescent molecules are nucleotide sequences specifically combined with the cytochrome c, and the nucleic acid aptamers marked on the gold nanorod particles are nucleotide sequences capable of being combined with the nucleic acid aptamers marked on the near-infrared organic molecules; incubating and mixing the detection agent and the cells for at least 20 hours, exciting by adopting near-infrared light after the cells uptake the nano detection system, and testing the fluorescence lifetime in a near-infrared window; and obtaining the content of the cytochrome c in the cell according to a pre-detected relationship between the fluorescence lifetime and the content of the cytochrome c.

The invention discloses a gene chip for detecting an environmental pollutant DEHP (di-2-ethylhexyl phthalate), and a preparation method and application thereof. The gene chip is characterized by comprising a probe 1, a probe 2 and a probe 3 of which the nucleotide sequences are disclosed as SEQ ID NO.1-3. The preparation method comprises the following steps: total RNA (ribonucleic acid) extraction, cDNA (complementary deoxyribonucleic acid) synthesis, PCR (polymerase chain reaction) amplification, product segment recovery and chip preparation, wherein the PCR amplification primers have amino acid sequences disclosed as SEQ ID NO.4-9. The application method comprises the following steps: after the sample and gene chip are hybridized, if the gene expressions corresponding to the probe 2 and probe 3 decrease and the gene expression corresponding to the probe 1 does not change obviously, the gene chip is responsive and sensitive to 0.4 mg / L DEHP; and if the gene expressions corresponding to the probe 1 and probe 3 increase and the gene expression corresponding to the probe 2 does not change obviously, the gene chip is responsive and sensitive to 4 mg / L DEHP. The gene chip has the advantages of high specificity, high sensitivity and high detection speed.

The invention discloses a primer group, a kit and a method for detecting six mouse viruses by multiple immunity fluorescence. The primer group is designed by the aid of multiple RT-PCR (reverse transcription-polymerase chain reaction) technologies and Luminex liquid chip technologies and can simultaneously detect six high-infection viruses such as TMEV, MHV, MNV, Reo-3, MVM and MPV of mice. By the aid of the primer group, target amplification products are acquired through specific PCR, the amplification products, fluorescence encoding microspheres and streptavidin-phycoerythrin are hybridized, and different types of viruses are distinguished when a mean fluorescence intensity value is read by a Luminex detector. The method has the advantages of rapidness, high efficiency, high specificity and sensitivity, good repeatability and the like and can be applied to quality monitoring, epidemiological investigation and early warning of test mice. The primer group is good in flexibility, and varieties detecting the viruses can be increased and decreased as required on the basis.

The invention relates to a method for detecting the activity of benzyl alcohol acetyltransferase. According to the method provided by the invention, a reaction mechanism that DTNB (5,5'-dithiobis-(2-nitrobenzoic acid) can have a mutual effect with sulfydryl to form colored thiophenol anions; the activity of the benzyl alcohol acetyltransferase in a protein sample is determined through detecting the generation amount of the sulfydryl in a reaction product. The method provided by the invention has the advantages that experiment operation steps are simple, the dosage of the sample is relatively less and a detection result is sensitive and accurate. When the method provided by the invention is applied, the efficient benzyl alcohol acetyltransferase can be relatively easily screened from different protein samples.

The invention relates to a method for detecting the activity of benzyl alcohol acetyltransferase. According to the method provided by the invention, a reaction mechanism that DTNB (5,5'-dithiobis-(2-nitrobenzoic acid) can have a mutual effect with sulfydryl to form colored thiophenol anions; the activity of the benzyl alcohol acetyltransferase in a protein sample is determined through detecting the generation amount of the sulfydryl in a reaction product. The method provided by the invention has the advantages that experiment operation steps are simple, the dosage of the sample is relatively less and a detection result is sensitive and accurate. When the method provided by the invention is applied, the efficient benzyl alcohol acetyltransferase can be relatively easily screened from different protein samples.

The invention discloses a method used for measuring content of furfural in transformer oil via high performance liquid chromatography. The method comprises following steps: 1, pretreatment of transformer oil, wherein oxidation of the transformer oil with an oxidizing agent is carried out, after oxidation, ethanol is taken as an extraction agent for extraction so as to obtain an extracted solution 1, and ethanol is also taken as an extraction agent to extract the untreated transformer oil so as to obtain an extracted solution 2; and 2, yield measurement of oxidation of the transformer oil into furfural, wherein the oxidation method and the oxidation conditions in the step 1 are adopted, and the reaction yield of oxidation of furfural in the transformer oil into furoic acid is carried out; 3, high performance liquid chromatography measurement, wherein a phenylpropyl bonded silica gel chromatographic column is adopted, a sodium citrate aqueous solution and methanol are taken as the mobile phase, wherein the pH value of the sodium citrate aqueous solution is controlled to be 4 to 5; and 4, measurement of furfural content. The method can be used for measuring furfural in transformer oil accurately.