Method for detecting patulin in apple juice

Abstract

The invention discloses a method for detecting patulin in apple juice. The method for detecting the patulin in the apple juice comprises the following steps that A, the concentrated apple juice is diluted; B, a liquid sample is obtained and is placed in a centrifugal pipe; C, an ethyl acetate / normal hexane extracting solution is added to the liquid sample, and then anhydrous sodium sulfate is added to the liquid sample; D, a cover of the centrifugal pipe is tightened, the centrifugal pipe is oscillated, then ultrasonic waves and vortexes are applied to the centrifugal pipe, and centrifugation is conducted; E, 2.5 ml of organic liquid is placed in the centrifugal pipe, and blowing is conducted for five to eight minutes by introducing nitrogen to the centrifugal pipe; F, water is added to the organic liquid added to the centrifugal pipe in the step E, and then ultrasonic waves are applied to the centrifugal pipe; G, the liquid sample obtained in the step F is placed in a sample bottle, an internal label solution is added to the sample bottle, and a sample is obtained after purification; H, a solution is extracted from the sample obtained in the step G, detection is conducted through a liquid chromatogram series mass spectrometer in the multi-reaction detection mode, and then detection is completed. According to the method, both time and labor are saved during detection, production cost is reduced, a detection result is more accurate, and detection sensitivity is higher.

Description

technical field [0001] The invention relates to the application field of a method for determining patulin, in particular to a method for determining patulin in apple juice. Background technique [0002] Patulin is a class of toxic compounds in mycomycins. It is highly toxic to animal cells and tissues, and has potential carcinogenicity, teratogenicity and mutagenicity. It mainly exists in apples, pears, grapes, etc. Waiting for fruits and vegetables. [0003] At present, for the detection method of patulin in apple juice, it often requires very cumbersome steps and needs to use a large amount of auxiliary solution reagents and solid samples, which not only makes the operation steps time-consuming and laborious, but also increases the amount of reagents and Samples increase the cost of production and processing. At the same time, this detection method also has defects such as inaccurate detection results and insensitivity. Contents of the invention [0004] In order to so...

AI Technical Summary

Problems solved by technology

[0003] At present, for the detection method of patulin in apple juice, it often requires very cumbersome steps and needs to use a large amount of auxiliary solution reagents and solid samples, which not only makes the operation steps time-consuming and laborious, but also increases the amount of reagents and Samples increase the cost of production and processing. At the same time, this detection method also has defects such as inaccurate and insensitive test results.