206results about How to "High infection rate" patented technology

Novel burst-free, sustained release biocompatible and biodegrable microcapsules which can be programmed to release their active core for variable durations ranging from 1-100 days in an aqueous physiological environment. The microcapsules are comprised of a core of polypeptide or other biologically active agent encapsulated in a matrix of poly(lactide / glycolide) copolymer having a molar composition of lactide / glycolide from 90 / 10 to 40 / 60, which may contain a pharmaceutically-acceptable adjuvant, as a blend of uncapped free carboxyl end group and end-capped forms ranging to ratios from 100 / 0 to 1 / 99.

A system and a method of computer controlled irrigation and fertigation composed of one or more sensors positioned in order to quantify the amount of water and / or nutrients that a plant is consuming. By controlling the fertigation, the plant or a part thereof, has improved yield and quality,

The invention relates to a method for growing blueberry, which comprises soil modification, seedling cultivation, seedling planting and fertilization. In the invention, a soil modifier is applied to soil and the organic materials in the soil modifier increase the content of fatty acids such as acetic acid and propionic acid of the soil, so the rate of mycorrhizal infection, which is capable of promoting the growth of the blueberry, improving blueberry yield, contributing to productivity improvement and soil modification and improving quality and sugar content of fruits, of the blueberry is improved; meanwhile, pine soil and grass carbon are used to regulate the pH value of the soil. In the invention, a rooting medium which contains IAA growth hormone and glycine betaine is used to cultivate the seedlings, so the rooting rate of cuttings and transplanting survival rate are improved, the seedling cultivation time is reduced greatly, and the propagation efficiency is improved obviously. In the invention, a microbial fertilizer, a potassium sulfate type compound fertilizer, ammonium sulfate and diammonium phosphate are used for fertilizing the blueberry, wherein the ammonium sulfate can lower the pH value of the soil, namely the ammonium sulfate provides nutrients for the blueberry for growth and improves the growing environment of the blueberry; and thus, the blueberry yield is more improved effectively.

A surgical anchor is provided with one or more anchor holes distributed around the head of the anchor. Each anchor hole is inclined to allow attachment of one or more sutures to the anchor either before, during or after the anchor is seated in a bone. The upper and lower apertures of each anchor hole are chamfered, and the chamfered areas from the anchor hole to the outside edge shall be polished or somehow smoothed to remove sharp edges and rough areas which may cause friction and abrasion to soft tissue or suture material. The lower surface of the anchor head may be angled to further simplify the task of feeding a surgical needle through the anchor hole after the anchor is seated into the bone.

The invention relates to a Beaueria bassaria(Balsamo)Vuillemin SCWJ-2 strain and a culture method thereof, a spore powder prepared from the strain and application of the spore powder in preparing a biocontrol preparation. The biocontrol preparation can be used for preventing and treating sweetpotato whitefly, Frankliniella occidentalis, Neotoxoptera formosana and / or peach aphid. The biocontrol preparation can be used for preventing and treating insects in a high-temperature environment, and has favorable preventing and treating effects; and the field control effect is higher than 75%.

The invention discloses a mycorrhizomata fostering method of Chinese and Indian mushroom, which comprises the following steps: collecting mushroom bacteria; gathering host plant seed such as Yunnan pine, mountain Hua pine and chestnut; disposing the host plant seed; breeding the host plant; inoculating the bacteria; cultivating the bacteria; detecting. The invention reduces the infectious rate of bacteria, which satisfies the living need with wide market prospect.

The invention discloses a method for improving growth and drought-resistant ability of wheat by using arbuscular mycorrhizal fungi to bring the reciprocal symbiosis relation between AM fungi and host plants under drought stress into play reasonably and efficiently. According to the method, the AM fungi having a close symbiotic relation with the wheat are selectively used, and the symbiotic relation between the AM fungi and the wheat is built in a condition control mode to promote the wheat growth and improve drought adaptability. The AM fungi comprise one of glomus monosporum and acaulospora laevis, the two kinds of AM fungi are respectively propagated in an expanding mode in an expanding propagation matrix by using red clovers, and then two AM fungi inoculants and the wheat are cultured together in a symbiotic culture medium. The effective path for profitable symbiosis of the AM fungi and the host plants in adversity conditions is provided, the infection and symbiosis relation with the host plants of the AM fungi is improved in a man-made mode, and the yield of dry crops is improved.

The invention relates to a Beaueria bassaria (Balsamo) Vuillemin bacterial strain GZGY-1-3, a culture method of the bacterial strain, spore powder prepared from the bacterial strain and application of the spore powder in preparing a biocontrol agent, wherein the biocontrol agent can be used for preventing and controlling bemisia tabaci, frankliniella occidentalis, green onion aphidand / or green peach aphid. The biocontrol agent can be used for preventing and controlling insects under a high temperature environment, and is good in prevention and control effect with field control efficiency over 75%.

The invention discloses an arbuscular mycorrhizal (AM) fungal inoculant. The AM fungal inoculant is prepared by the following steps: taking white clover seeds and dwarf lilyturf seeds as the host plants, taking glomus mosseae, glomus reticulatum, glomus etunicatum, or glomus intraradices as the inoculant, carrying out culture under specific nutritional conditions, cutting off the parts above the root of plants in the harvest period, grinding the roots and matrix parts, evenly mixing so as to obtain the AM fungal inoculant. According to the preparation method, white clover and dwarf lilyturf are combined according to a specific ratio, the combination is taken as the carrier for fungal propagation, and the propagation carrier is cultured under specific nutritional conditions so as to produce AM fungal inoculant having the advantages of high infection rate and large spore density. The quality of AM fungal inoculant is the best, when glomus mosseae is taken as the inoculant, the infection rate is the highest, and the spore density is the largest. The AM fungal inoculant can be widely applied to scientific research, ecological control, soil improvement, and the like.

The invention relates to a method for the horizontal transfection of bemisia tabaci of the exogenous endosymbiotic bacteria, which pertains to the field of agricultural biotechnology. The preparation method includes: the purification of the exogenous endosymbiotic bacteria Wolbachia, the collection and fixation of a bemisia tabaci receptor, the horizontal transfection of the exogenous endosymbiotic bacteria Wolbachia to the bemisia tabaci receptor, the collection and fixation of an injection individual and the detection of the endosymbiotic bacteria. The invention is simple and quick, which can successfully transfect the exogenous endosymbiotic bacteria to a bemisia tabaci individual, and a bemisia tabaci offspring has higher infection rate.

The invention discloses a sand seedling planting method. The sand seedling planting method includes the following steps of (1) soaking bare roots of seedlings to be planted in water for 6-12 hours, (2) mixing clayed soil and water according to the volume ratio of 1:3, and adding arbuscular mycorrhizal fungi in the mixture to form a slurry, wherein the mass ratio of the mixture and the arbuscular mycorrhizal fungi is 100:5-10, (3) dipping the bare roots of the seedlings to be planted in the slurry of step (2) for 3-5 minutes after the bare roots are soaked in the step (1), and then planting. Experimental results show that compared with a traditional planting method, the method is used for planting the seedlings in sand soil, the infection rate of mycorrhizae can be improved by 10%, and the surviving rate of the seedlings can be improved by 20-30%. Therefore, the sand seedling water-retaining inoculation planting method has important practical significance in sand ecological restoration.

The invention relates to methods and kits for assessing the risk, for an individual, of developing an adverse event upon treatment with a therapeutic antibody which is capable of Fab-arm exchange, said method comprising the steps of: a) providing a sample from an individual who is a candidate for treatment with said therapeutic antibody, b) assaying said sample for the presence of circulating IgG4 antibodies that binds an antigen known or suspected to be associated with a causative agent of said adverse event, and c) assessing, on the basis of the outcome of the assay of step b), the risk that the individual will develop said adverse event upon treatment with the therapeutic antibody, wherein the risk of development of said adverse event increases with increased level of said circulating IgG4 antibodies.

The invention discloses an improved efficient white muscardine silkworm cultivating method. The method includes the following steps that silkworms are bred, beauveria bassiana spore suspension is sprayed on the surfaces of the silkworms within the 0-2h after the four-instar moulting silkworms molt, the silkworms die due to attack of diseases after five days to six days, the dead silkworms are picked out for further muscardine, and the silkworms are aired or dried to obtain muscardine silkworms. The varieties of the silkworms are Haoyue, Taiyuan and Hang 7. The silkworm varieties poor in tolerance of beauveria bassiana are screened out to serve as the varieties special for white muscardine silkworm production, so that the infection rate of the silkworms is improved. The extreme early stage of the five-instar silkworms newly molting is selected as the optimal period for inoculating the silkworms with the beauveria bassiana, and therefore the infection rate of the silkworms and the yield of the white muscardine silkworms are increased.

The invention provides a preparation method of cordyceps militaris nano powder, comprising the following steps: 1) separating wild cordyceps militaris paecilomyces, and purifying to prepare cordyceps militaris strain liquor; 2) selecting health and live insect pupae, sterilizing by ozone for later use; 3) infecting the insect pupae sterilized in step 2) by the strain liquor prepared by step 1); 4) performing bionic cultivation on infected insect pupae obtained in step 4) to obtain cordyceps militaris cells integrating pupalcells and sporocarps into a whole; and 5) carrying out microwave drying and nano grinding on the obtained cordyceps militaris cells cultured in step 4) to obtain the cordyceps militaris nano powder. The preparation method of the invention has low investment and low energy consumption, the prepared cordyceps militaris nano powder is not polluted by sterilization process, and the fineness of the powder can reach nano level, thus the powder can keep original functionalactivity and is easily absorbed by a human body.

The invention refers to the technical field of soil recovery, and particularly relates to a method for repairing soil heavy metal pollution by synergy of plants, mycorrhiza and earthworm. The method comprises the following steps: inoculating mycorrhiza fungi to the soil polluted by heavy metal, and then planting specific plants in the soil; at last, inoculating earthworm in the soil at the seedling stage of the plant, and forming a plants-mycorrhiza-earthworm system; repairing the soil. By adopting the plants-mycorrhiza-earthworm system, the technology of repairing soil heavy metal at an original position and a fixed point is established, and the purpose of repairing the farmland soil heavy metal pollution can be realized. The earthworm can improve the infection rate of the mycorrhiza, so as to enhance the repairing function of the mycorrhiza on the heavy metal polluted soil, lighten soil pollution and protect the farm crops from being polluted; and thereby, human health is protected; besides, the method is low in investment, easy to master the technology, high in product additional value, quick in investment return, and very suitable for our actual condition, and can better promote the soil sustainable use and development.

A method and apparatus for reattaching the opposed ends of a member, such as a tendon, ligament or bone, during preparing and healing of the member using a surgical repair device that can be securely attached to the member and then safely guided through tortuous anatomy for reattachment and repair. The repair device further includes structural means so as to secure opposed ends of the member against separation during healing. Devices for aiding in the positioning of the surgical repair device are provided.

The invention discloses a method for cultivating citrus container mycorrhiza seedlings by combined application of AMF and PGPR (Plant Growth Promoting Rhinoacteria) bacterium agents. The method comprises the following steps: preparing an AFM mycorrhiza inoculant, preparing a PGPR bacterium agent, disinfecting seedling culturing soil, accelerating germination of trifoliate orange seeds, sowing the seeds and inoculating. According to the method for cultivating the citrus container mycorrhiza seedlings, designed by the invention, the mycorrhiza infection rate of citrus root systems can be improved, nutrients of nutrient soil mineral substances can be activated very well, and the absorption and utilization of nutrients and moisture by the citrus container seedlings are promoted; the citrus container seedlings which have higher stress resistance and survival rate are cultivated; the ordered and rapid growth of industrialized large-batch container seedlings is facilitated, and the seedling culturing period is shortened; citrus trees can benefit from the mycorrhiza growth promoting effect in the whole life and the application amount of chemical fertilizers in the container seedlings is reduced.

The invention relates to a special matrix formula suitable for culturing mycorrhizal tobacco seedling in the condition of flue-cured tobacco seedling by floating system; all the constituents and the volume thereof are as follows: 60 percent of peat, 20 percent of perlite and 20 percent of vermiculite. The formula can improve the comprehensive quality of the tobacco seedling, cultures sound seedlings and has the advantages of wide material sources, easy acquisition, easy matrix preparation, simple production, simple inoculating operation, little fungicide dosage and easy promotion, etc. The culture medium, the inoculating technique and the corresponding Glomus intraradices Smith and Schenck are combined together, infection rate of mycorrhiza is up to over 40 percent and the effect of the mycorrhiza is good.

The invention discloses a cultivation method of Cordyceps militaris (pupa). The method includes the steps of 1, subjecting properly-aged live silkworms to crymo-anesthesia; 2, pouring pore suspension of Cordyceps militaris into the heads of the silkworms; 3, cultivating the treated and survived silkworms at 4-6 DEG C under the humidity of 65-75% until death and stiffing; 4, embedding the dead, stiff silkworms in sandy soil to cultivate for 6-8 days; 5, cultivating at 17-19 DEG C under the humidity higher than 75%, decreasing the humidity to 60-70% when obvious mycelia grow from the heads and primordium of fruiting bodies differentiates, continuing to cultivate until the length of the fruiting bodies on the heads of the silkworms grow to more than 2cm long, and taking out Cordyceps militaris (pupa) before drying. The method has the advantages that inoculation infection rate, stiffing rate and yield are high; nutrients of the obtained product are approximate to those of natural Cordyceps; the shape of the obtained product is approximate to that of wild Cordyceps; the fruiting bodies are full and golden yellow with uniform size, so that standard packaging is facilitated and added value of products is higher.

The invention provides a herpes simplex virus which is separated from yellow race. The virus has high infection rate on the specificity of the host cells of yellow race, has high reproduction speed; so that can be directly used for infecting and dissolve the tumour cells of yellow race, and can also be used as a carrier to carry gene medicine into the cells of yellow race to conduct gene treatment.

The present invention provides, among other things, implants including a biocompatible substrate, wherein the substrate includes a plurality of pores, such as trabecular and / or dodecahedral pores, and wherein said implant is configured as a transcutaneous implant. In some embodiments, provided implants may comprise multiple layers with differing pore densities, geometries, and / or distributions.

The invention belongs to the field of microbiology and provides a mycorrhizal fungi strain efficiently growing with a rhododendron seedling. The problems of the prior art that the seedling growth is slow, the death rate is high, the leaves are yellow, and the like, during the artificial seedling culturing process for the rhododendron are solved. The preservation number of the mycorrhizal fungi strain provided by the invention is CGMCCNo:6010; the mycorrhizal fungi strain belongs to ascomycetes, chactomium globosum, discellaceae, oidiodendron; the mycorrhizal fungi strain provided by the invention can efficiently grow together with the rhododendron seedling; the rhododendron seedling is inoculated in a seedling stage; the infection rate of mycorrhiza is high; the growth of the seedling is obviously boosted; the survival rate of the seedling is increased; and when the pH of soil is 5.5-7.2, the strain inoculated on the seedling can effectively promote the growth of the seedling.

The invention provides a herpes simplex virus carrier which is separated from the yellow race and lacks one or more than one gene of the gene of coded infection cell multi-peptide 34.5, the gene of coded infection cell multi-peptide 6 and the gene of coded infection cell multi-peptide 47; the virus carrier has high specificity infection rate to the host cells of the yellow race with high reproduction speed and low toxicity, so the virus carrier can be directly used for injecting and dissolving the tumor cells of the yellow race, and carry genomic medicine into the cells of the yellow race to have gene therapy.

The invention discloses a seedling culture device and method for culturing mycorrhizal seedlings. The device comprises an upper layer and a lower layer, a seedling culture chamber is formed in the upper layer, and an inoculant chamber is formed in the lower layer. An opening is formed in the upper portion of the seedling culture chamber, the bottom of the seedling culture chamber is in a screen shape, and a PVC screen or an iron wire screen with the hole diameter being 2 mm is arranged at the bottom of the seedling culture chamber. An opening is formed in the upper portion of the inoculant chamber, the bottom of the inoculant chamber is sealed, and an arbuscular mycorrhizal fungal inoculant is put in the inoculant chamber. During seedling culture, the surfaces of seeds are disinfected with10% sodium hypochlorite or other disinfectants, and the surfaces of the seeds are completely washed with sterile water. A culture matrix or soil is subjected to hot-pressing sterilization, and then sowing and seedling culturing are carried out. The mycorrhizal seedlings are cultured through the seedling culture device, the seedling culture matrix is not mixed with the arbuscular mycorrhizal fungal inoculant, the inoculant only makes contact with root systems of the seedlings, the density of the inoculant around the root systems is increased, the root systems fully make contact with the inoculant, the infection rate of mycorhiza is increased, infection of other infectious microbes is reduced, the use quantity of the seedling culture matrix can be reduced, and the cost can be reduced.

The invention belongs to the field of botany, and provides a method for the quick mycorhiza formation of azalea aseptic seedlings. By the method, the problems of high death rate, low growth speed, yellowing of laminae and the like of azalea seedlings in the prior art are solved. The method comprises the following steps of: preparing a cultivation medium and a nutrient solution; and transplanting rooted seedlings which are sown or subjected to tissue culture into the cultivation medium which is subjected to aseptic treatment, inoculating at least one bacterial block, and placing the seedlings in a culture chamber for culturing, wherein the cultivation medium consists of grass carbon and yellow sand in a weight ratio of (2-3):1; in the nutrient solution, the concentration of glucose is 0.1 to 0.3 percent; and the bacterial block is selected from any one of CGMCC NO: 6010 or 6031, and the inoculating position of the bacterial block is 0.5 to 1.0 centimeter away from a root system. By themethod, mycorrhiza of the azalea seedlings can be formed quickly and is high in infection rate, and the growth of the seedlings is quick, so the method can be used for scientific study and also can be used for the production of annual seedlings of azalea.

The invention discloses a method for earthing-cultivating silkworm insect grass by simulating an ecological environment. The method comprises the following steps: (1) selecting a strain host; (2) enlarging preparation solution of cultivated strain liquid; (3) preprocessing and postprocessing inoculation; (4) preparing an embedding substrate; and (5) management of earthing-cultivating: inoculatingthe strains optimized in the step (2) in a fluid nutrient medium added with 5-8 wt% of silkworm chrysalis meal, after enlarging the cultivation, inoculating in healthy silkworm larvae in the step (1)for infecting and rigidifying, and embedding infected and rigidified silkworm bodies to be developed in the step (3) in a transparent plastic box filled with the plant ash embedding substrate; after sporophore is grown to be matured, taking out the silkworm bodies from the plant ash embedding substrate, and cleaning up plant ash adhered to the periphery of the silkworm bodies, to obtain the matured silkworm insect grass. The method has the characteristics of low sundry fungus contamination rate, high infection rigidifying rate, high production rate, good stability of the obtained silkworm insect grass, higher yield, low cost, and full and regular appearance of the silkworm insect grass, is suitable for the large-scale production, and has the extensive development prospect.

The invention provides a method for preparing AMF (Arbuscular Mycorrhizal Fungus) capsule fungicide. The method is characterized in that sodium alga acid is used as a basic carrier, a mixture is obtained by matching and mixing multiple fillers such as chitosan, gelatin, glycerol, rice, peat, coal cinder and malt sprout in the basic carrier, a fungicide mixture can be obtained by embedding the fungicide in the mixture after the mixture is sterilized, a spherical object can be prepared by dripping the fungicide mixture into calcium chloride solution in a physical extrusion way, and the AMF capsule fungicide can be obtained after the spherical object is solidified and cleaned. According to the method disclosed by the invention, spores, mycorrhiza and hypha of inner glomus intraradices fungus are embedded in the sodium alga acid, a complete infection process can be completed when the spores, the mycorrhiza and the hypha are seeded in unpasteurized soil, and the infection rate can be increased with the prolonging of the infection time. The method disclosed by the invention has the advantages of simpleness, low cost and low toxicity; an embedding material of the AMF capsule fungicide prepared by the invention can be easily decomposed by microorganism, the preservation time is longer, and the AMF capsule fungicide can be simultaneously and directly seeded in a big field.