Preparation method of cordyceps militaris nano powder
A technology of Cordyceps militaris and nanopowder, applied in food preparation, application, food science, etc., can solve the problems of easy denaturation and high production cost, and achieve the effects of preserving biological activity, increasing output rate, and high infection rate
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Embodiment 1
[0031] 1) Collect wild Cordyceps militaris in Changbai Mountains, isolate Paecilomyces militaris species, carry out liquid expansion, shaker culture for 7 days, and obtain Paecilomyces militaris species liquid.
[0032] 2) Inoculate the strain prepared in step 1) on tussah silkworm chrysalis soaked in 0.7ppm ozone water for 15 minutes. The inoculation point is from the tail to the head of the pupal body, and each silkworm chrysalis is inoculated with 0.4ml.
[0033] 3) After inoculation, under the conditions of 25°C, 1000 Lx light, and 12 hours of light per day, culture for 60 days, the fruiting body of Cordyceps militaris grows and obvious ascus shells are formed on the upper part of the fruiting body, that is, high-quality Cordyceps militaris is cultivated.
[0034] 4) Harvest the Cordyceps militaris obtained in step 3), place them in a microwave tunnel with a wavelength frequency of 2450 MHz, and a temperature of 50°C to 60°C to dry until the moisture content reaches 7%.
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Embodiment 2
[0038] 1) Collect silkworm pupae and soak them in 0.8ppm ozone water for 10 minutes.
[0039] 2) Inoculate the sterilized silkworm chrysalis with the Paecilomyces militaris seed solution prepared in step 1) of Example 1. The inoculation point is from the tail to the head of the pupal body, and each silkworm chrysalis is inoculated with 0.2ml of the bacterial solution.
[0040] 3) After inoculation, culture for 70 days under the conditions of 25°C, 1000 Lx light, and 12 hours of light per day. When the fruiting body of Cordyceps militaris grows and obvious ascus shells are formed on the upper part of the fruiting body, high-quality mulberry pupae are obtained. Cordyceps.
[0041] 4) Harvest the mulberry Cordyceps militaris obtained in step 3), place it in a microwave tunnel with a wavelength frequency of 2450 MHz and a temperature of 50°C to 60°C, and take it out when the moisture content is 8%.
[0042] 5) The dried Cordyceps militaris in step 4) is coarsely crushed to 40 ...
Embodiment 3
[0044] 1) Collect tussah silkworm chrysalis and soak them in 1.0ppm ozone water for 20 minutes.
[0045] 2) Inoculate the sterilized tussah silkworm chrysalis with the Paecilomyces militaris solution prepared in step 1) of Example 1. The inoculation point is from tail to head at the pupal body, and each silkworm chrysalis is inoculated with 1.0 ml of the solution.
[0046] 3) After inoculation, culture for 80 days under the conditions of 18°C, 1000 Lx light, and 12 hours of light per day. When the fruiting body of Cordyceps militaris grows and obvious ascus shells are formed on the upper part of the fruiting body, high-quality tussah pupae are obtained. Cordyceps.
[0047] 4) After harvesting the Cordyceps militaris cultivated in step 3), place it in a microwave tunnel with a wavelength frequency of 2450MHz and a temperature of 50°C~60°C to dry until the moisture content is 5%.
[0048] 5) The dried tussah Cordyceps militaris obtained in step 4) is coarsely pulverized to 20...
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