44results about How to "Preserve biological activity" patented technology

The invention discloses a novel chondrocyte epimatrix membrane and a preparation method thereof. The membrane contains cell epimatrix ingredients which have bioactivity and cell activity, wherein the cell epimatrix comprises the following main ingredients in percentage by weight: 80 to 95 percent of bionic type II collagen in an acellular cartilage matrix and 10 to 20 percent of hyaluronic acid, 10 to 20 percent of aminopolysaccharide and the like which serve as a chondrocyte epimatrix; the chondrocyte epimatrix biological membrane prepared from the acellular cartilage matrix has the bionic chondrocyte epimatrix ingredients, is excellent in cell consistency, does not arouse immunological rejection of host cartilage tissue, can be used for repairing the damage of hyaline cartilage tissue of joints and reconstructing the hyaline cartilage tissue, and also can be used for a seed cell inoculating vector and a growth factor sustained-release vector; and the chondrocyte epimatrix contains blood vessel inhibiting factor ingredients, so the membrane also can be used for preventing the conglutination of tissue such as muscle tendons.

The invention discloses freeze-dried powder for repairing acne marks and scars. The freeze-dried powder is prepared by the steps of: A, preparing raw materials; B, dissolving mannitol, oligopeptide-3, disodium hydrogen phosphate and oligopeptide-2 in water at normal temperature, placing the solution in a 121 DEG C autoclave, and performing sterilization at a constant temperature for 20 minutes to form a solution A; C, sequentially adding sodium dihydrogen phosphate and serum albumin into the solution A, shaking the mixture uniformly, and performing filtration by using a filter membrane to obtain a solution B; D, standing the solution B for 18-36h, and then performing filling; E, performing freeze drying on the solution B after filling; and F, packaging the product after drying. The freeze-dried powder prepared by the invention can achieve multiple repair and protection of a skin damaged by acnes, and can promote skin microcirculation and improve the acne marks to ensure that the skin can recovered to a healthy state.

The invention relates to an ovarian large cortex piece vitrified cryopreservation protection liquid and a cryopreservation method thereof. Although the vitrified cryopreservation method is widely applied to experimental animals, a universal cryopreservation method and a standard cryopreservation liquid preparation scheme are absent at present, so that the sized transplantation subjected to vitrified cryopreservation is at a small-size stage, an ovarian small cortex piece has limited follicles, and the service life of the transplanted follicles is influenced. The invention provides vitrified cryopreservation protection liquid suitable for an ovarian large cortex piece, and gonadotropin is added on the basis of the common vitrified liquid. According to the vitrified cryopreservation protection liquid suitable for the ovarian large cortex piece, especially the ovaries of experimental animals, improved variety livestock and wild endangered species in sudden death are conveniently cryopreserved at the most proper osmotic equilibrium time of performing vitrified cryopreservation on the ovarian large cortex pieces of different sizes; and moreover, according to the method, the survival rate of the follicles can be improved, the blood reperfusion time of the transplant is shortened, and the survival rate of the cryopreserved ovarian transplantation in vivo is improved.

The invention discloses a preparation method for abelmoschus esculentus proteoglycan protein xerium, which includes the steps: 1) adding water into abelmoschus esculentus raw materials, crushing the abelmoschus esculentus raw materials with a tissue destructor, adding compound enzyme for enzymolysis and extraction prior to centrifugally removing large residue particles, and performing suction filtration to obtain abelmoschus esculentus proteoglycan protein extracting solution; and 2) adding maltodextrin into the prepared abelmoschus esculentus proteoglycan protein extracting solution, and obtaining the target product, namely the abelmoschus esculentus proteoglycan protein xerium by means of high-pressure homogenization and spray drying. As tested, essential components of the abelmoschus esculentus proteoglycan protein xerium refer to soluble polysaccharide and protein, wherein the content of the polysaccharide is >=75wt%, the content of the protein is >=10wt%, and the moisture content is >=4.2wt%. The abelmoschus esculentus proteoglycan protein xerium can effectively improve the human gastrointestinal function, and is high in active component and fine in physical characteristic, and can serve as raw materials to fill capsules or be tableted so as to produce functional food.

The invention provides a preparation method of cordyceps militaris nano powder, comprising the following steps: 1) separating wild cordyceps militaris paecilomyces, and purifying to prepare cordyceps militaris strain liquor; 2) selecting health and live insect pupae, sterilizing by ozone for later use; 3) infecting the insect pupae sterilized in step 2) by the strain liquor prepared by step 1); 4) performing bionic cultivation on infected insect pupae obtained in step 4) to obtain cordyceps militaris cells integrating pupalcells and sporocarps into a whole; and 5) carrying out microwave drying and nano grinding on the obtained cordyceps militaris cells cultured in step 4) to obtain the cordyceps militaris nano powder. The preparation method of the invention has low investment and low energy consumption, the prepared cordyceps militaris nano powder is not polluted by sterilization process, and the fineness of the powder can reach nano level, thus the powder can keep original functionalactivity and is easily absorbed by a human body.

The invention provides a method for separating and purifying cobra neurotoxin protein through dual-ion exchange chromatography. The method comprises the following steps of: (1) first separation and purification through ion exchange chromatography, namely, a) dissolution of crude cobra venom, and b) separation and purification through an SP-Sephodex-C25 gel column; and (2) second separation and purification through ion exchange chromatography, namely, a) balance of a SourceS(XK5030) column having a column volume of 500 ml by using 1000 ml of liquid A for future use; and b) SourceS(XK5030) column purification, and then collection of the cobra neurotoxin protein purified twice according to a standard purification chromatogram. The method provided by the invention is scientific and rational in process; and no organic reagent, no high-concentration salt and no protein modification method are used in production, so that the biological activity of cobratide can be maintained to an utmost extent. The method provided by the invention is advanced in process and simple to operate; the production period can be greatly shortened, the production time can be saved and the production cost can be reduced; and the method is completely suitable for an industrial production line.

The present invention discloses a novel anti-crop-epiphyte polypeptide and a preparation method thereof. A recombined anti-crop-epiphyte polypeptide is formed by linking gene which codes white candida pheromone and the gene which codes colicin. Compared with the prior anti-crop-epiphyte medicine, the novel anti-crop-epiphyte polypeptide of the present invention has the advantages that the novel anti-crop-epiphyte polypeptide directly forms ionic passage at epiphytic cell membrane to kill the epiphyte, so the killing efficiency is thousands times stronger than the prior anti-crop-epiphyte medicine; the epiphyte can hardly amend the geometrical damage at the cell membrane caused by the polypeptide by the mutation-expression form, so the polypeptide can not induce the epiphyte producing the traditional drug resistance; the polypeptide is a biological preparation and has target killing towards the epiphyte, so the novel anti-crop-epiphyte polypeptide does not have the toxic and side effects of the prior anti-crop-epiphyte medicine.

The invention discloses a plaster. The plaster is characterized in that the plaster comprises giant knotweed rhizome, clematis root, Chinese starjasmine stem, Szechuan lovage rhizome, doubleteeth pubescent angilica root, obscured homalomena rhizome, rhizoma corydalis, common burreed rhizome, zedoary tumeric, frankincense, myrrh, rhubarb, garden balsam stem, sargentgloryvine stem, cassia bark, fortune's drynaria rhizome, radix salviae miltiorrhizae, fourstamen stephania root, ground beetle, dahurian angelica root, gansui root, euphorbiales, nux vomica, common monkshood mother root, kusnezoff monkshood root, pedate pinallia jackinthepulpit rhizome, white mustard seeds, motherwort herb, dried ginger, pangolin scales, dragon's blood, radix notoginseng, acacia catechu, borneol, camphor and calcined calamine. The plaster comprises the following components in units by weight: 200 units of rhizoma corydalis, 200 units of frankincense, 300 units of myrrh, 50 units of euphorbiales, 60 units of borneol, 60 units of camphor and 120 units of calcined calamine. The plaster has the advantages that 1) the plaster is prepared by adding a modern advanced purification and extraction process on the basis of a traditional black plaster and then taking rosin as a matrix for improvement, and has the characteristics of no smell, no skin irritation, no allergy and high medicament loading amount; and 2) the plaster has significant curative effects on treatment of rheumatoid bone diseases, traumatic injuries, traumas, dysmenorrhea and other disease symptoms.

The invention relates to a production method of quickly-fermented high-umami seafood fermented soybean sauce, including; preparing small-molecular fish protein enzymatic hydrolysate by means of efficient biological enzymatic hydrolysis, and jointly fermenting through the small-molecular fish protein enzymatic hydrolysate with soybean meal and bran to obtain the seafood fermented soybean sauce. The method is low in cost and has a short production cycle, only 30 d, umami of the obtained soybean sauce can be improved, added value of low-value fishes can also be improved, low-value marine animal resources can be comprehensively utilized, and innovation is also made to zero addition of flavorings; meanwhile, the method of the invention is different from common low-salt solid fermented soybean sauces, the addition of the fish protein enzymatic hydrolysate enables the seafood soybean sauce to be healthy, environment-friendly, low in cost, good in property and excellent in umami, and compared with like products, the seafood soybean sauce will be popular with consumers owing to the excellent flavor.

The invention relates to a method for analyzing traditional Chinese medicine dimethyl sulfoxide extracting solution by a nuclear magnetic resonance technology. The invention is characterized in that the method comprises the following steps: preparing sample solution, weighting traditional Chinese medicines or Chinese herbal preparation according to the regulation, carrying out pretreatment on the traditional Chinese medicines or the Chinese herbal preparation, using dimethyl sulfoxide to replace an original extracting agent according to the extraction dosage specified in the same item, extracting and filtering by an extraction method frequently-used for traditional Chinese medicine at the temperature of 18.45 DEG C to 189 DEG C to obtain the sample solution, adopting a conventional nuclear magnetic resonance system, selecting parameters of each system according to the nuclear magnetic resonance system and the characteristics of the traditional Chinese medicine, determining the content of the sample solution qualitatively and quantitatively, thereby obtaining a nuclear magnetic resonance spectrum containing all compositions of the traditional Chinese medicine, a molecular structure and physical characteristics. In the method, the dimethyl sulfoxide is used as the extracting agent for extracting the traditional Chinese medicines and the Chinese herbal preparation; the method can totally extract all water soluble, oil soluble, volatile compositions of the traditional Chinese medicine at the room temperature of over 18.45 DEG C by an ultrasonic method, can avoid the volatilization, hydrolyzation, oxidation, denaturation of active ingredients and preserves the bioactivity of the active ingredients to the greatest extent. The method is simple, convenient and safe to operate and has short extraction time.

A miniature polypeptide resisting to EB virus tumor, its gene and its recombinant plasmid are disclosed. Its preparing process includes linking the gene for coding and leading peptide with the plasmid carrying the colicin ion channel structure domain gene, transferring engineering bacteria for amplifying, and separating and purifying by His-tag column. It can be used to kill tumor cells.

The embodiment of the invention discloses a wrinkle-removing composition. The wrinkle-removing composition comprises wrinkle-removing composition freeze-dried powder and a solvent, wherein the wrinkle-removing composition freeze-dried powder is prepared from, by mass, 0.001-2% of palmityl tripeptide-1, 0.01-2% of palmityl pentapeptide-4, 0.001-5% of collagen and 91-99.7% of mannitol. The wrinkle-removing composition comprises the wrinkle-removing composition freeze-dried powder, through the preservation of the freeze-dried powder, the bioactivity of polypeptide can be maintained to the greatest extent, and the best effect is achieved. The freeze-dried powder composition and the solvent are separately preserved, and when the wrinkle-removing composition is needed, what is only needed is touniformly mix the freeze-dried powder composition and the solvent. According to the wrinkle-removing composition, expression wrinkles and physiological static wrinkles can be inhibited and desalinatedsimultaneously, and the skin collagen is directly and quickly supplemented, so that the skin gets tightened and smooth.

The present invention relates to freeze dried powder for injection with ginseng stem and leaf extract as main material and its preparation process. The freeze dried powder for injection may be used in treating coronary heart disease and climacteric syndrome, and can strengthen physique and strengthening immunity. It contains ginseng stem and leaf extract in 3-20 weight portions and supplementary material and water in 25-250 weight portions. The preparation process includes adding supplementary material and water into ginseng stem and leaf extract through stirring to dissolve, packing, and freeze drying via quick lowering temperature to -40 to -55 deg.c and pumping to 5-10 Pa, heating to 25-40 deg.c and maintaining for 5-20 hr. The preparation process has less damage of the effective medicine components and stable product quality, and the product has long preservation period and other advantages.

The invention discloses a method for preparing active collagen polypeptides from fresh pig skin. The method comprises the following preparation steps of 1, selection of a material, wherein the fresh pig skin which is qualified in inspection and quarantine is selected and purchased from a regular large meat factory on the market; 2, rough processing of the pig skin, wherein the selected and purchased pig skin is preliminary processed; 3, cleaning of the pig skin, wherein the pig skin after being cut flat is cleaned by adopting a mode of combining mechanical and physical methods; 4, enzymatic hydrolysis of the pig skin, wherein the clean pig skin after shredding and cleaning is placed in an enzymatic hydrolysis reactor, and pure water is added into the enzymatic hydrolysis reactor accordingto the amount of the pig skin; 5, purification separation and concentration, wherein an enzymatic hydrolysate obtained after the enzymatic hydrolysis of the pig skin material is filtered through a filter cloth; 6, drying, wherein a collagen polypeptide concentrate after concentration is placed in a front box of freeze-drying equipment for freeze-drying for 40-50 hour; smashing and packaging.

The invention discloses a preparation method of passion fruit shell extracts. The method mainly comprises the following steps of peeling passion fruit shells; performing drying, grinding and sieving;weighing a certain amount of passion fruit shell powder; performing steam-microwave two-section respective extraction; finally merging collected precipitates and concentrates; next, performing freeze-drying; obtaining the passion fruit shell extracts.

The invention discloses a preparation method of a multifunctional biological chip. The preparation method comprises the following steps: step one, manufacturing an adhesive layer to obtain the adhesive layer with a through hole; step two, fixing the adhesive layer on a substrate; step three, fixing the AAO film on the base through the adhesive layer, wherein the through hole location of the adhesive layer coincides with a center point of the AAO film; step four, depositing gold-nano-particles on the AAO film; step five, taking off an Au-AAO film with the gold-nano-particles from the substrate;step six, dropwise adding PEG buffer solution at one side of the Au-AAO film with gold-nano-particles; step seven, keeping the Au-AAO film dropwise added with the PEG buffer solution in dark place toobtain a PEG-Au-AAO film for manufacturing a biological chip after modifying the biomolecule. The prepared biological chip prepared through the preparation method disclosed by the invention can quickly detect the specific protein or extracellular vesicles while separating the specific protein or extracellular vesicles, and the operation is simple and efficient. The preparation process is free from using the organic chemical reagent, the pollution on the protein and extracellular vesicles is avoided, the biological activity can be saved, and a use demand on the biological application field issatisfied.

The present invention relates to recipe and making process of one health food with shallot bulb as main material. The health food is prepared with shallot bulb in 55-98 wt% and one of fleeceflower root, notoginseng and red sage in 0.1-45 wt%, and through freeze drying, superfine crushing and other steps. It is prepared into tablet, granule, capsules or other form. It has the functions of regulating blood fat and preventing cardiac and cerebral vascular diseases.

The invention relates to folium mori polysaccharide, a separation and purification preparation method thereof and an application of folium mori polysaccharide in preparation of anticoagulant healthcare products and medicine. The preparation method of folium mori polysaccharide comprises following steps: folium mori is subjected to smashing, aqueous extraction and alcohol precipitation, passes a polyamide column for deproteinization, is subjected to H2O2 decoloration and then is subjected to separation and purification by a DEAE-sepharose CL-6B ion exchange column and a sepharose CL-6B gel column to obtain folium mori polysaccharide. Further classified separation and purification of folium mori polysaccharide are realized, a pure product of folium mori polysaccharide with an average molecular weight of (4.5-8.0)*10<4>Da is obtained, and experiments prove that folium mori polysaccharide has high coagulation activity, is safe and non-toxic, and can be developed with other raw materials and auxiliary materials which are acceptable in healthcare product and medicine processes to form healthcare products and medicine with the good anticoagulant function, so that folium mori polysaccharide has good development and application prospect.

The invention relates to a biological gel implant for maxillary sinus and a preparation method of the biological gel implant. A porous surface structure and an internal hollow structure are manufactured by adopting a 3D printing technology combined with a CNC (Computer Numerical Control) method, wherein a lot of biological gel with osteoinductive factors is stored in the porous surface structure and the internal hollow structure; the biological gel implant comprises an implant section, a drill base, a center screw, biological gel and an aseptic packaging bottle; the top end of the implant section is connected with the drill base by virtue of screws; an outside cap is arranged at the top of the implant section; the neck of the implant section is provided with micro threads; the lower part of the implant section is of a porous surface structure and an internal hollow structure; a texture surface is formed in the hole wall; the porous structure is communicated with the internal hollow structure; a lot of biological gel with osteoinductive factors can be stored; and the concentration and release time of the biological gel are much superior to those of a biological coating implant at present. The biological gel implant has a skeleton-like hollow structure capable of inducing bone tissues to actively grow into the implant, the new bone fusion area and bone-formation strength of the implant can be greatly increased, and the biological gel implant is suitable for a maxillary sinus implant surgery under the condition of osteoporosis.

The invention provides a method for adding natural astaxanthin to baked food. The method comprises the following steps: S1, feeding the laying hen with the haematococcus pluvialis algae powder as a raw material, and obtaining the astaxanthin egg rich in natural L-esterified astaxanthin; S2, using astaxanthin egg as a base material, processing the material into mayonnaise, egg yolk powder and egg butter rich in animal astaxanthin under low temperature conditions; S3, astaxanthin egg yolk powder: directly mixing the egg yolk powder obtained in S2 with the flour, and performing operation according to the normal operation process; S4, astaxanthin mayonnaise: directly mixing the mayonnaise obtained in S2 with flour, or being used for post-moulding and seasoning after setting the noodles; S5, astaxanthin egg butter: directly mixing the egg butter obtained in S2 with cream and an oily filler of salad oil; and S6, astaxanthin egg: breaking the astaxanthin egg obtained in S1 and directly mixingthe material with the flour, and performing operation according to the normal operation process. The method ensures better biological activity, avoids fishy smell and improves body absorption rate.

The present invention provides one kind of fusion protein, which consists of one chain avidin and one interleukin-2 connected through one junctional peptide in the amino acid sequence of SerSerGlyGlySerGlyGlyGlyGlySerGlyGlyGlyGly Ser. The fusion protein has the activity of both chain avidin and interleukin-2, and can have its interleukin-2 anchored to the surface of biotinylated tumor cell by means of the powerful joint between the chain avidin and the biotin and exist stably on the surface of gamma ray deactivated tumor cell while maintaining the activity of interleukin-2. The tumor vaccine surface modified with the fusion protein has the functions of preventing and treating tumor.

The present invention relates to freeze dried powder for injection prepared with the extract of root bark and stem bark of girald daphne. The preparation process is used in treating gall, stabbing pain, psychroalgia, etc. of limbs and trunk. It is prepared through a percolation process including extracting barked root and stem of girald daphne, filtering, decompression distillation to concentrate while recovering alcohol, re-filtering, regulating pH to 6.5-7.5 and resulting in mannitol concentration in the solution of 50-200mg / ml, filtering for the third time, and freeze drying. The freeze dried powder for injection has high curative effect and fast acting, and the low temperature vacuum drying process avoids the decomposition and oxidation of effective medicine components and results in high medicine stability.

The invention relates to a health care type bath liquid containing the extract of walnut green husk, which pertains to the plant extract bath liquid taking surface active compounds as base materials.The invention smashes the fresh walnut green husk after flushing thereof, then diluted ethanol solution is used immediately for leaching, and the extract can be obtained by rotating evaporation and concentration; then surfactant, cleaning agent, flavor, deionized water, etc. are utilized to be prepared into the walnut green husk bath liquid. The bath liquid of the invention contains plant polyphenols and other plant chemical ingredients which are beneficial to human body, and the state of the invention is semi-fluid colloidal liquid with light brown and dark green luster. The invention can bepreserved for a plurality of months without adding preservative, which has good effects of decontaminability, foaming property, skin clean and skin care, thus providing a way to make use of the resources of walnut green husk.

The invention discloses a kit for determining an amino-terminal pro-brain natriuretic peptide. The kit is characterized by comprising a reagent card, a reaction substance and a reaction buffer solution, a detection line of the reagent card is coated with an amino-terminal pro-brain natriuretic peptide monoclonal antibody, and a quality control line of the reagent card is coated with one of a goatanti-chicken IgY antibody, a goat anti-rabbit IgG antibody or a goat anti-mouse IgG antibody. Before the kit is used, a reaction buffer solution is adopted to redissolve a reaction substance, and thena sample to be detected is added so that the amino-terminal pro-brain natriuretic peptide in the sample to be detected fully reacts with the amino-terminal pro-brain natriuretic peptide monoclonal antibody marked by the fluorescent microspheres, and the kit is high in detection accuracy, strong in specificity, good in reproducibility and rapid to operate.

The invention relates to a grinding device suitable for frozen tissue. The grinding device suitable for the frozen tissue comprises a base, wherein a tool accommodating box is arranged in the base, adrawer-type powder collecting box with an open upper part is arranged on the base, a low-temperature crushing box body penetrating up and down is arranged on the upper part of the powder collecting box, dry ice-type low-temperature ice bags are arranged in four side walls of the box body, a drawable screen is arranged at the bottom of the box body, a sealing cover is arranged at the top end of thebox body, a feeding port is fixedly arranged on the sealing cover, the top end of the feeding port is hinged with a sealing feeding cover, a grinding and crushing mechanism is horizontally arranged in the middle of the box body, and the grinding and crushing mechanism is manually driven to realize low-temperature crushing on the frozen tissue. The grinding device suitable for the frozen tissue has the advantages of simple structure and ingenious design, ensures the activity of an experimental sample, reduces the loss of a tissue sample, protects the safety of experimental personnel, and greatly improves the grinding efficiency.

The invention discloses a novel chondrocyte epimatrix membrane and a preparation method thereof. The membrane contains cell epimatrix ingredients which have bioactivity and cell activity, wherein the cell epimatrix comprises the following main ingredients in percentage by weight: 80 to 95 percent of bionic type II collagen in an acellular cartilage matrix and 10 to 20 percent of hyaluronic acid, 10 to 20 percent of aminopolysaccharide and the like which serve as a chondrocyte epimatrix; the chondrocyte epimatrix biological membrane prepared from the acellular cartilage matrix has the bionic chondrocyte epimatrix ingredients, is excellent in cell consistency, does not arouse immunological rejection of host cartilage tissue, can be used for repairing the damage of hyaline cartilage tissue of joints and reconstructing the hyaline cartilage tissue, and also can be used for a seed cell inoculating vector and a growth factor sustained-release vector; and the chondrocyte epimatrix contains blood vessel inhibiting factor ingredients, so the membrane also can be used for preventing the conglutination of tissue such as muscle tendons.

The invention provides a fusion protein. The fusion protein is composed of strepavidin and soluble CD40L which are bonded by a joint peptide, wherein the amino acid sequence of the joint peptide is Thr Ser Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Glu Phe. The fusion protein has the combined activities of both the strepavidin and the CD40L; the CD40L can be anchored on the surface of biotinylated tumor cell through the strong bonding between the strepavidin and biotin and exist stably on the surface of the tumor cell inactivated by gamma rays while retaining the activity of the CD40L. The fusion protein has a function of treating tumor by locally anchoring tumor and can be used for preparing vaccine for preventing and treating tumor.