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192results about How to "Preserve activity" patented technology

RNA interference mediated inhibition of MAP kinase gene expression or expression of genes involved in MAP kinase pathway using short interfering nucleic acid (siNA)

The present invention concerns methods and reagents useful in modulating MAP kinase gene expression in a variety of applications, including use in therapeutic, diagnostic, target validation, and genomic discovery applications. Specifically, the invention relates to small nucleic acid molecules, such as short interfering nucleic acid (siNA), short interfering RNA (siRNA), double-stranded RNA (dsRNA), micro-RNA (miRNA), and short hairpin RNA (shRNA) molecules capable of mediating RNA interference (RNAi) against c-JUN, JNK, p38, and ERK gene expression, useful in the treatment of cancer, inflammation, obesity and insulin resistance (e.g. Type I and Type II diabetes).
Owner:MCSWIGGEN JAMES +5

RNA interference mediated inhibition of cholesteryl ester transfer protein (CEPT) gene expression using short interfering nucleic acid (siNA)

This invention relates to compounds, compositions, and methods useful for modulating cholesteryl ester transfer protein (CETP) gene expression using short interfering nucleic acid (siNA) molecules. This invention also relates to compounds, compositions, and methods useful for modulating the expression and activity of other genes involved in pathways of CETP gene expression and / or activity by RNA interference (RNAi) using small nucleic acid molecules. In particular, the instant invention features small nucleic acid molecules, such as short interfering nucleic acid (siNA), short interfering RNA (siRNA), double-stranded RNA (dsRNA), micro-RNA (miRNA), and short hairpin RNA (shRNA) molecules and methods used to modulate the expression of CETP genes.
Owner:SIRNA THERAPEUTICS INC

Sub-critical fluid extraction solvent and abstraction method

The present invention relates to a dissolvent with effective ingredient extracted from the plant-and-animal raw material and the extraction method thereof. One subcritical fluid extraction solvent includes an extraction solvent of a compound or composition extracted from the raw material containing compound or composition as component element, and the solvent is mainly characterized in that the liquid sulfur hexafluoride works as the extraction solvent. The invention also includes a method for extracting the subcritical fluid extraction solvent. The advantage of the invention is that the extraction and separation of the target component in the natural product are optionally executed to further increase the extraction rate of the intermediate product and the usage factor of the raw material while the unique dissolving character of the sulfur hexafluoride and the mixed solvent thereof to the liposoluble active ingredient is used. The physical features of strong dissolving capacity and low boiling point of the sulfur hexafluoride and the mixed solvent thereof are used to reduce the consumption quantity of the energy source, dissolvent of the system and the cost, and increase the market competition ability of the intermediate product.
Owner:韩延欣 +2

Method for preparing fibrin ferment-chitosan self-assembly nano particle and use thereof

The invention relates to a method for preparing thrombin-chitosan self-assembly nano particles and application thereof. The method comprises: adding chitosan into an acetic acid aqueous solution to form a chitosan solution; adding thrombin into a buffer solution to form a thrombin solution; uniformly mixing the thrombin solution and the chitosan solution, adding a crosslinking agent for stirring, pouring the mixture into liquid paraffin for stirring, adding a dispersant, and performing emulsification; and adding the crosslinking agent for crosslinking reaction through water bath, performing filtration and acetone washing, using ethanol for washing and dehydration, and finally performing vacuum drying to obtain the thrombin-chitosan self-assembly nano particles. The method feeds the thrombin and the chitosan according to proportion to prepare thrombin-chitosan which is self-assembled into nano particles for the first time, maintains the stability of the thrombin of the thrombin-chitosan self-assembly nano particles, and gives better play to the comprehensive hemostatic effect of the thrombin and the chitosan of the thrombin-chitosan self-assembly nano particles. The method can be widely applied to tissue hemostasis, tissue sealing and tissue adhesion in the surgical fields such as general surgery, orthopaedics, heat and chest surgery, neurosurgery and gynaecology and obstetrics.
Owner:GUANGZHOU BIOSEAL BIOTECH

Novel dispersant, preparation method thereof and application thereof

The invention discloses a preparation method of a novel dispersant, which comprises the steps of: taking crylic acid as a main reaction monomer; adding at least one of monomers such as methacrylic acid, maleic anhydride, fumaric acid, itaconic acid and methyl sodium allylsulfonate as comonomer; taking r-methacryloxypropyl trimethoxy silane, vinyl trimethoxy silane or vinyl triethoxysilane as organic silicon monomer to participate in polymerization; taking a redox system as an initiating system; and polymerizing by water solution, and neutralizing by alkaline substance to obtain the novel dispersant. Due to the novel dispersant, the grinding of calcium carbonate has the characteristics of being low in sizing agent viscosity, small in grain size, good in dispersibility and high in stability.
Owner:山东永港伟方环保材料科技有限公司

Plant drying and essential oil and hydrolat extraction system based on air energy

The invention relates to a plant drying and essential oil and hydrolat extraction system based on air energy.The plant drying and essential oil and hydrolat extraction system comprises a drying oven, a condenser, an oil-water separator and an air energy heating and cooling unit.The drying oven is used for drying plants and volatilizing moisture and essential oil in the plants to form oil-water gaseous state mixture.The condenser is connected with the drying oven and used for lowering the temperature of the gaseous state mixture and converting the gaseous state mixture into liquid state mixture.The oil-water separator is used for separating the liquid state mixture into essential oil and hydrolat.The air energy heating and cooling unit heats the drying oven and cools the condenser.Compared with the prior art, the system improves device production efficiency and greatly lowers device energy consumption; due to high system integration, the device site requirements are lowered, the device structure is simplified, the device investment is lowered, the device operation difficulty and maintenance cost are lowered, and environmental pollution caused by exhaust emission is avoided.
Owner:上海依肯机械设备有限公司

RNA Interference Mediated Inhibition of Adenosine A1 Receptor (ADORA1) Gene Expression Using Short Interfering Nucleic Acid (siNA)

The present invention concerns methods and reagents useful in modulating adenosine A1 receptor (ADORA1) gene expression in a variety of applications, including use in therapeutic, diagnostic, target validation, and genomic discovery applications. Specifically, the invention relates to small interfering RNA (siRNA) molecules capable of mediating RNA interference (RNAi) against ADORA1 and related receptors.
Owner:SIRNA THERAPEUTICS INC

Saliva protection agent

InactiveCN105039306AWidening the scope of protection conditionsGuaranteed integrity and long-term stabilityMicrobiological testing/measurementDNA preparationAmmonium sulfateChemistry
The invention relates to the field of biology, and in particular relates to a saliva protection agent as well as a preparation method and application thereof. Each 1000ml of the saliva protection agent consists of 10-50mmol of tris-hydroxymethyl aminomethane, 0.5-20mmol of ethylenediaminetetraacetic acid and / or sodium ethylene diamine tetracetate, 200-1000mmol of ammonium sulfate and 1-50mmol of glucose. Saliva, preserved in the saliva protection agent disclosed by the invention, is nearly free from change in the first 60 days, still complete in DNA and free from obvious degradation; the saliva is remained so after 6 months; and after 12 months, the saliva still remains the integrity of DNA as before. The saliva protection agent disclosed by the invention can be used for quite effectively preserving a saliva sample and for guaranteeing the integrity and long-term stability of the sample.
Owner:SHANGHAI MAJORBIO BIO PHARM TECH

Low-temperature refining method of tea seed oil

InactiveCN101914413AAchieve the purpose of refiningHigh yieldFatty-oils/fats refiningFatty acidImpurity
The invention discloses a low-temperature refining method of tea seed oil, comprising an absorbing and refining step and a filtering step. The invention adopts the core technology of utilizing a mixed absorption agent to absorb and filter impurities in crude oil, such as free fatty acid, various pigments, phospholipid, and the like to achieve the aim of refining the tea seed oil so as to substitute traditional procedures, such as degumming, deacidifying, bleaching, deodorizing, and the like. The invention adopting a low-temperature physical absorption technology only needs an absorbing and refining pot and filtering equipment, reduces the traditional refining procedures, shortens the production period, lessens the refining loss of the tea seed oil, avoids the execution of the high-temperature process of the traditional refining process, reserves biological active substances and nutrient contents in the tea seed oil, such as vitamins, squalene, and the like and improves the product quality and the health function. The tea seed oil obtained through refining is colorless and transparent and has high yield, equipment investment is small, and the method is simple and suitable for industrial production, keeps the pure natural characteristics of the tea seed oil and greatly improves the added values of the tea seed oil.
Owner:FUJIAN NORMAL UNIV

Microcapsule peruvian ginseng tea and preparation method thereof

InactiveCN105053325AImprove eating experienceExpand the scope of application and consumption methodsPre-extraction tea treatmentTea substituesChemical preservativesActive component
The invention discloses a microcapsule peruvian ginseng tea and a preparation method thereof. The microcapsule peruvian ginseng tea comprises the following components in parts by weight: 10-50 parts of peruvian ginseng raw powder, 0.52-2.61 parts of a microcapsule wall material, 1-20 parts of a reorganizing pelleting agent, and 10-100 parts of tea leaves or a tea replacement beverage. The preparation method of the microcapsule peruvian ginseng tea comprises the following steps: S1, microencapsulated embedding; S2, pelleting composite slow-release particles; and S3, compounding peruvian ginseng composite slow-release particles, the tea leaves or the tea replacement beverage, and mixing the peruvian ginseng composite slow-release particles, the tea leaves or the tea replacement beverage so as to obtain finished products. The microcapsule peruvian ginseng tea prepared by the preparation method completely preserves the overhead price nutrition and the active components of the peruvian ginseng raw powder, does not contain any chemical preservatives or pigment, and is a natural green health-care beverage.
Owner:JINDA BIOENG BIOTECH DEV FUZHOU

Method to produce a medicinal product comprising a biologically active protein and the resulting product

The present invention pertains to a method for producing a medicinal product comprising a biologically active protein comprising the steps of providing an aqueous composition comprising a solvent, the biologically active protein and between 20% w / w and 60% w / w of a non-polymeric sugar, freezing the composition, thereby forming at least one frozen body comprising the solvent in frozen form, putting the frozen body in a drying apparatus while being carried by a support, the support comprising one or more restraining elements that define one or more boundaries of the support, wherein at most 30% of the surface of the body is contiguous with the one or more restraining elements, reducing the pressure in the drying apparatus below atmospheric pressure, providing heat to the body in order to sublimate the frozen solvent of the body and obtain a dried body. The invention also pertains to a product obtainable by this method.
Owner:INTERVET INC

High-pressure homogeneous honey and preparation method for product thereof

The invention provides high-pressure homogeneous honey and a preparation method for a product thereof. Specifically, the method comprises the following steps: crushing and filtering pure honey or royal jelly, pollen and bee pupa respectively, taking filter liquor of any one or a filter liquor mixture of any two or any three of the materials to be mixed with 80 to 95 percent honey after vacuum concentration at the temperature of between 10 DEG C below zero and 30 DEG C, decompressing and stirring the honey or the mixture repeatedly, and delivering the honey or the mixture into a high-pressure homogenizer of 70MPa to be compressed and homogenized to be even. The royal jelly, the pollen, the bee pupa and the honey are stirred in vacuum and then are compressed and homogenized by adopting the high-pressure homogenizer of 70MPa to form a stable combination so as to be prepared into nutrient honey. The method adopts the high-pressure homogenizer of 70MPa and uses different parameter indexes to achieve the aims of improving the grain diameter and granularity size of the honey, improving the refined mouthfeel of the honey, enhancing the absorption of the human body to nutrient substances, realizing the cooperative application of the high-pressure homogenizing technology and the prior ultra-filtration and purification technology, and greatly improving the quality of the honey.
Owner:CHENGDU JIANSHENGTANG IND

Process for extracting both active embryonic peptide and natural corn oil from embryo bud of corn

InactiveCN1396175AImprove physiological function and nutritional potencyGood economic benefitsPeptide preparation methodsPlant peptidesPelletizingPeptide
A process for preparing the active embryonic peptide with coproduct of corn oil from the embryo bud of corn at the same time includes such steps as wet and thermal pretreating, grinding by colloid mill, multi-stage zymolysis, centrifugal separation to obtain fibres, corn oil and milk, concentrating the milk by nanometre filtering membrane, further concentrating, homogenizing, emulsifying, drying and granulating.
Owner:徐昌洪

Preparation of umbilical cord mesenchymal stem cell factors with hair growth effect

The invention relates to the field of cytokine germination, in particular to preparation of umbilical cord mesenchymal stem cell factors with hair growth effect. The preparation includes the followingsteps: primary isolation and culture of human umbilical cord mesenchymal stem cells, collection of a cell culture supernatant, centrifugation, ultrafiltration and concentration to obtain a liquid containing the umbilical cord mesenchymal stem cell factor complex, and a freeze-drying process to prepare umbilical cord mesenchymal stem cell factor lyophilized powder. By the preparation method of theumbilical cord mesenchymal stem cell factors, activity of the cell factors can be preserved to the maximum and the cell factors are convenient to store and use. Hair follicle cells are fundamentallyactivated, and the hair regeneration effect is promoted remarkably and is more durable.
Owner:陕西中鸿科瑞再生医学研究院有限公司

Health-care noodles containing canna edulis ker and processing method thereof

InactiveCN104068324AGreat tasteHigh phosphorus and bound phosphorus contentDough treatmentFood preparationAdditive ingredientClean water
The invention discloses health-care noodles containing canna edulis ker and a processing method thereof. The health-care noodles containing the canna edulis ker, provided by the invention, are prepared by adding 5-50 parts of canna edulis ker starch and 0.1-1 part of alkaline powder into every 100 parts of flour. The processing method comprises the following steps: mixing the canna edulis ker starch, the flour and the alkaline powder according to the proportion of the formula, and adding clean water, wherein the proportion of the clean water to mixed powder is 5:2; putting the mixture into a dough mixer to be blended evenly, mixing the mixture into a dough, curing the dough for 30 minutes at the environment of 25 DEG C and the humidity of 30%, sending the dough to a noodle press to be pressed into a flat cake, and cutting the flat cake into strips to make wet noodles; and drying, cutting off and packaging the wet noodles to obtain the health-care noodles containing the canna edulis ker. The processing method comprises the steps of mixing ingredients, mixing flour, curing, tabletting, extending, cutting into strips, drying, cutting off and packaging. Compared with general noodles, the health-care noodles containing the canna edulis ker, disclosed by the invention, are better in mouth feel, are richer in nutrition, are good in product quality, are stable in quality, and are better pleased and welcomed by consumers; at the same time, the health-care noodles containing the canna edulis ker, disclosed by the invention, have the characteristics of simple processing technology and low production cost.
Owner:NANJING LEHEHE MANOR AGRI SCI & TECH
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