Preparation method of 3D tissue engineering cell ring

Abstract

The invention discloses a preparation method of a 3D tissue engineering cell ring. By utilizing a relatively narrow growth space and a surface non-adhesion characteristic of a culture mold, added cells are self-assembled into a ring in a culture solution for promoting cell aggregation and leave the surface of a cell culture medium, and a macroscopic tissue block or organoid with a three-dimensional structure is formed. A naturally-formed extracellular matrix exists between the cells prepared by the method, the exogenous scaffold interference does not exist, and the communication and the information exchange between the cells are more complete and direct so as to reduce the difference between a culture environment and an in-vivo environment and more truly simulate an in-vivo cell growth environment. Moreover, the prepared cell ring is a cell body with a regular annular structure, can simulate the in-vivo cell microenvironment, links a 2D cell culture system with the research of tissuesand organs, and is beneficial to research of organoids.

Description

technical field [0001] The invention relates to the field of cell engineering, in particular to a method for preparing 3D tissue engineering cell rings. Background technique [0002] Traditional medical research and cell biology research mostly adopt the two-dimensional cell culture model, which provides a flat culture plane. However, under physiological conditions, tissue cells grow in three-dimensional space, and cells not only contact each other, but also contact with supporting structures such as extracellular matrix. The two-dimensional cell culture model cannot really reflect the extracellular matrix in the tissue, so many physiological reactions in this model, such as receptor expression, transcription expression, cell migration and apoptosis, have been confirmed to be consistent with those that occur in actual organs or tissues. The situation is different. Moreover, the entire process of cell division, proliferation, migration and apoptosis is precisely regulated, ...

AI Technical Summary

Problems solved by technology

Two-dimensional cell culture models are still far from accurately reproducing the function of cells in tissues

The cell scaffold is mainly to construct a porous structure in three-dimensional space for cell attachment and growth. Cells attach to the scaffold for three-dimensional growth and migration. The main scaffold materials include collagen and hydrogel. The three-dimensional culture technology that does not require scaffolds is mainly through Physical methods suspend adherent cells in the medium to achieve the purpose of three-dimensional culture. At present, the main technologies include microcarrier, magnetic levitation, hanging drop plate and magnetic three-dimensional bioprinting. These methods are relatively complicated to operate and require a large initial investment. At present, the vast majority of three-dimensional cultures rely on scaffold materials

The current hanging drop culture method can only grow microspheres with a particle size of hundreds of microns, and its geometric size is far from the real 3D tissue structure.

In addition, the space occupation of the gel or scaffold affects the formation of a regular and dense three-dimensional structure of the cells, and its degradation products also affect the growth and differentiation of the cells. The high cost is also a disadvantage, and it is still not an ideal three-dimensional culture strategy.

Most of the above methods still have problems such as complicated operation process, scaffolds affecting cell growth and differentiation, small cultured cells, and inconvenient follow-up operations. There is still a big gap from the ideal 3D cell culture model, and most of the current products come from foreign companies. , the product is expensive, which limits its wide application in China and hinders the development of domestic medical research

At present, there are no competing 3D cell culture solutions and products in China

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