Arbuscular mycorrhizal fungal inoculant

Abstract

The invention discloses an arbuscular mycorrhizal (AM) fungal inoculant. The AM fungal inoculant is prepared by the following steps: taking white clover seeds and dwarf lilyturf seeds as the host plants, taking glomus mosseae, glomus reticulatum, glomus etunicatum, or glomus intraradices as the inoculant, carrying out culture under specific nutritional conditions, cutting off the parts above the root of plants in the harvest period, grinding the roots and matrix parts, evenly mixing so as to obtain the AM fungal inoculant. According to the preparation method, white clover and dwarf lilyturf are combined according to a specific ratio, the combination is taken as the carrier for fungal propagation, and the propagation carrier is cultured under specific nutritional conditions so as to produce AM fungal inoculant having the advantages of high infection rate and large spore density. The quality of AM fungal inoculant is the best, when glomus mosseae is taken as the inoculant, the infection rate is the highest, and the spore density is the largest. The AM fungal inoculant can be widely applied to scientific research, ecological control, soil improvement, and the like.

Description

technical field [0001] The invention relates to a microbial inoculum, in particular to an arbuscular mycorrhizal fungus inoculum. Background technique [0002] Arbuscular mycorrhiza is the most widespread symbiont between plant roots and fungi in nature. Arbuscular mycorrhizal fungi are the most widely distributed type of fungi in soil symbiotic fungi, and can form fungi with most land plants in nature. root. Arbuscular mycorrhiza can not only promote the absorption of mineral elements and water by plants, but also significantly improve the stress resistance and disease resistance of plants, which is beneficial to the growth and development of plants, and is of great significance to agricultural production and desertification control. For example, after AM fungi were inoculated on the roots of field crops such as soybeans, wheat, and cotton, as well as fruit tree crops such as apples and citrus, the stress resistance and yield of plants were significantly improved. In addi...

AI Technical Summary

Problems solved by technology

[0003] However, at present, due to the obligate living symbiotic nature of AM fungi, AM fungi cannot be isolated and purely cultured so far, which greatly limits the application of AM fungi.

At this stage, there are many methods for the propagation of AM fungi, such as living host pot culture method, glass bead compartment culture method, tissue culture method, etc. The advantages of glass bead compartment culture method and tissue culture method are that they can combine culture substrate with AM fungi are separated to obtain pure AM fungal cells; however, its technical content is relatively high, and it is only used for scientific research and small-scale inoculant production; the advantage of using live host plant pot culture method to cultivate AM fungi is that it is easy to operate and simple Reliable, it is currently the main method for pure culture of AM fungi, but the dissemination rate and spore density of the AM inoculum prepared by the current pot culture method are generally low, and the quality of the inoculum is poor, which cannot meet the needs of the industry

Images