Seedling culture device and method for culturing mycorrhizal seedlings

Abstract

The invention discloses a seedling culture device and method for culturing mycorrhizal seedlings. The device comprises an upper layer and a lower layer, a seedling culture chamber is formed in the upper layer, and an inoculant chamber is formed in the lower layer. An opening is formed in the upper portion of the seedling culture chamber, the bottom of the seedling culture chamber is in a screen shape, and a PVC screen or an iron wire screen with the hole diameter being 2 mm is arranged at the bottom of the seedling culture chamber. An opening is formed in the upper portion of the inoculant chamber, the bottom of the inoculant chamber is sealed, and an arbuscular mycorrhizal fungal inoculant is put in the inoculant chamber. During seedling culture, the surfaces of seeds are disinfected with10% sodium hypochlorite or other disinfectants, and the surfaces of the seeds are completely washed with sterile water. A culture matrix or soil is subjected to hot-pressing sterilization, and then sowing and seedling culturing are carried out. The mycorrhizal seedlings are cultured through the seedling culture device, the seedling culture matrix is not mixed with the arbuscular mycorrhizal fungal inoculant, the inoculant only makes contact with root systems of the seedlings, the density of the inoculant around the root systems is increased, the root systems fully make contact with the inoculant, the infection rate of mycorhiza is increased, infection of other infectious microbes is reduced, the use quantity of the seedling culture matrix can be reduced, and the cost can be reduced.

Description

technical field [0001] The invention relates to the technical field of seedling breeding, in particular to a seedling raising device and method for cultivating mycorrhized seedlings. Background technique [0002] Mycorrhizal seedlings refer to adding mycorrhizal fungal agents to the seedling substrate or rhizosphere soil when cultivating seedlings, so that mycorrhizal fungi can infect the roots of seedlings and form mycorrhizal symbionts, which are successfully infected by mycorrhizal fungi. Seedlings are mycorrhizal seedlings. The use of mycorrhizal seedlings in afforestation has the advantages of high survival rate, fast growth rate, and strong stress resistance. Mycorrhizal fungi are divided into ectomycorrhizal fungi and endomycorrhizal fungi. Endomycorrhizal fungi are also called vesicular arbuscular mycorrhizal fungi, or arbuscular mycorrhizal fungi for short. Ectomycorrhizal fungi mainly infect a few higher plants such as Pinaceae and Fagaceae, while endomycorrhizal...

AI Technical Summary

Problems solved by technology

There are still some defects in the mycorrhizal seedlings cultivated by this method: (1) The seedling substrate needs to be sterilized before cultivating the mycorrhizal seedlings. Due to the large amount of seedling substrate required, the sterilization cost is high

However, most of the afforestation seedlings cannot be obtained through tissue culture and rapid propagation technology at present. They are mainly obtained by growing seedlings in seedbeds or nutrient cups. In this case, more seedling substrates are used, and the cost of sterilization is high.

(2) At present, arbuscular mycorrhizal fungi cannot reproduce in vitro, and the propagation period of the bacterial agent is long. If seedlings are raised in a seedbed or a nutrient cup, a large proportion of the inoculated bacterial agent needs to be added to the soil matrix, and a large dose of the bacterial agent is required. The cost is high, and the arbuscular mycorrhizal fungus can only infect the plant root system through the full contact of the mycelia and spores with the plant root system. When using a seedbed or a nutrient cup for seedling cultivation, the density of the fungus around the root system is small, and the spores and mycelium cannot communicate with the root system. Full contact will greatly affect the inoculation effect, and even cannot infect the root system

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