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Method for cultivating citrus container mycorrhiza seedlings by combined application of AMF and PGPR (Plant Growth Promoting Rhinoacteria) bacterium agents

A technology of combined application and mycorrhizal seedlings is applied in the field of citrus seedling cultivation, which can solve the problems of inability to meet the needs of large-scale seedling cultivation, low efficiency of single microorganism growth-promoting effect, etc. The effect of reducing the dosage

Active Publication Date: 2016-02-17
ZHEJIANG CITRUS RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, years of research have shown that the growth-promoting efficiency of a single microorganism is generally low, which cannot meet the actual needs of large-scale seedling cultivation.

Method used

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  • Method for cultivating citrus container mycorrhiza seedlings by combined application of AMF and PGPR (Plant Growth Promoting Rhinoacteria) bacterium agents
  • Method for cultivating citrus container mycorrhiza seedlings by combined application of AMF and PGPR (Plant Growth Promoting Rhinoacteria) bacterium agents
  • Method for cultivating citrus container mycorrhiza seedlings by combined application of AMF and PGPR (Plant Growth Promoting Rhinoacteria) bacterium agents

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] The AMF mycorrhizal inoculum is formulated with Moses capsule mold agent, and contains 25 mature and healthy spores per gram of substrate.

[0038] The PGPR bacterial agent is a liquid bacterial agent of Paenibacillus colloidus or Bacillus megaterium with a concentration of 10 8 CFU / ml.

[0039] Superphosphate is not added in the described nursery soil.

[0040] Comparison of the growth of Aurantium citrus seedlings without inoculation, single inoculation with AMF and joint inoculation with AMF and PGPR at 5 months (significant difference P<0.05).

[0041]

[0042] Note: Different letters after the values ​​in the table indicate significant differences (LSD analysis), the same below.

Embodiment 2

[0044] The AMF mycorrhizal inoculum is formulated with the root capsule mold agent, and contains 25 mature and healthy spores per gram of substrate.

[0045] The PGPR bacterial agent is a liquid bacterial agent of Paenibacillus colloidus or Bacillus megaterium with a concentration of 10 8 CFU / ml.

[0046] Superphosphate is not added in the described nursery soil.

[0047] Comparison of the growth of Aurantium citrus seedlings without inoculation, single inoculation with AMF and joint inoculation with AMF and PGPR at 5 months (significant difference P<0.05).

[0048]

Embodiment 3

[0050] The AMF mycorrhizal inoculum is formulated with Moses capsule mold agent, and contains 25 mature and healthy spores per gram of substrate.

[0051] The PGPR bacterial agent is a liquid bacterial agent of Paenibacillus colloidus or Bacillus megaterium with a concentration of 10 8 CFU / ml.

[0052] Add 0.75% superphosphate in the described nursery soil.

[0053] Comparison of the growth of Aurantium citrus seedlings without inoculation, single inoculation with AMF and joint inoculation with AMF and PGPR at 5 months (significant difference P<0.05).

[0054]

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Abstract

The invention discloses a method for cultivating citrus container mycorrhiza seedlings by combined application of AMF and PGPR (Plant Growth Promoting Rhinoacteria) bacterium agents. The method comprises the following steps: preparing an AFM mycorrhiza inoculant, preparing a PGPR bacterium agent, disinfecting seedling culturing soil, accelerating germination of trifoliate orange seeds, sowing the seeds and inoculating. According to the method for cultivating the citrus container mycorrhiza seedlings, designed by the invention, the mycorrhiza infection rate of citrus root systems can be improved, nutrients of nutrient soil mineral substances can be activated very well, and the absorption and utilization of nutrients and moisture by the citrus container seedlings are promoted; the citrus container seedlings which have higher stress resistance and survival rate are cultivated; the ordered and rapid growth of industrialized large-batch container seedlings is facilitated, and the seedling culturing period is shortened; citrus trees can benefit from the mycorrhiza growth promoting effect in the whole life and the application amount of chemical fertilizers in the container seedlings is reduced.

Description

technical field [0001] The invention relates to a citrus seedling cultivation technique, in particular to a method for cultivating citrus container mycorrhizal seedlings by combined application of AMF and PGPR bacterial agents. Background technique [0002] Arbuscular mycorrhizal fungi (AMF) exist in almost all ecological types of soil and are extremely widely distributed in nature. They can infect 80% of terrestrial higher plants to form mutualistic symbionts. Studies have shown that AMF fungi can promote the absorption of nutrients and water by host plants, and improve the resistance of host plants to various biotic and abiotic stresses; in addition, they can bioremediate degraded soil, improve soil structure, reduce soil pollution, protect and The use of resources and other aspects also play an irreplaceable important role. [0003] The rhizosphere growth-promoting bacteria (PGPR) are an important class of beneficial microorganisms in the rhizosphere soil microecology. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G17/00A01C1/02C12N1/14C12R1/645
CPCA01C1/02A01G17/005C12N1/14
Inventor 王鹏王引吴韶辉温明霞石学根
Owner ZHEJIANG CITRUS RES INST
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