Cultivation method of Cordyceps militaris (pupa)
A cultivation method and technology of Cordyceps silkworm, applied in the field of cultivation of Cordyceps silkworm, can solve the problems of inappropriate cultivation conditions, cocooning and pupation, low rigidity rate, etc. Effect
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Embodiment 1
[0022] (1) Selection of live silkworms
[0023] Select 3-5 instar silkworms, the bigger the better.
[0024] (2) Preparation of Cordyceps militaris spore suspension
[0025] The Cordyceps militaris strains covered with mycelium were eluted with sterile water to make a spore suspension with a concentration of 200 mg / mL, and stored at low temperature for future use.
[0026] (3) Hypothermic anesthesia before inoculation
[0027] Live silkworms are placed in a constant temperature incubator at 4-6°C for 1 day without feeding during the period, so that the silkworms enter a semi-dormant state due to low temperature and hunger, and their life activities are reduced.
[0028] (4) Inoculation
[0029] Use a sterilized syringe to extract the spore suspension, carefully pierce it through the head of the silkworm, and slowly inject about 2 mL. And spray a small amount of spore suspension on the silkworm body surface.
[0030] The experimental results showed that the inoculation inf...
Embodiment 2
[0036] (1) Selection of live silkworms
[0037] Select 3-5 instar silkworms, the bigger the better.
[0038] (2) Preparation of Cordyceps militaris spore suspension
[0039] The Cordyceps militaris strains covered with mycelium were eluted with sterile water to make a spore suspension with a concentration of 300 mg / mL, and stored at low temperature for later use.
[0040] (3) Hypothermic anesthesia before inoculation
[0041] Live silkworms are placed in a constant temperature incubator at 4-6°C for 2 days without feeding during the period, so that the silkworms enter a semi-dormant state due to low temperature and hunger, and their life activities are reduced.
[0042] (4) Inoculation
[0043] Use a sterilized syringe to extract the spore suspension, carefully pierce it through the head of the silkworm, and slowly inject about 3 mL. And spray a small amount of spore suspension on the silkworm body surface.
[0044] The experimental results showed that the inoculation inf...
Embodiment 3
[0050] (1) Selection of live silkworms
[0051] Select 3-5 instar silkworms, the bigger the better.
[0052] (2) Preparation of Cordyceps militaris spore suspension
[0053] The Cordyceps militaris strains covered with mycelium were eluted with sterile water to make a spore suspension with a concentration of 400 mg / mL, and stored at low temperature for future use.
[0054] (3) Hypothermic anesthesia before inoculation
[0055] Live silkworms are placed in a constant temperature incubator at 4-6°C for 3 days without feeding during the period, so that the silkworms enter a semi-dormant state due to low temperature and hunger, and their life activities are reduced.
[0056] (4) Inoculation
[0057] Use a sterilized syringe to extract the spore suspension, carefully pierce it through the head of the silkworm, and slowly inject about 4 mL. And spray a small amount of spore suspension on the silkworm body surface.
[0058] The experimental results showed that the inoculation in...
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