Eureka AIR delivers breakthrough ideas for toughest innovation challenges, trusted by R&D personnel around the world.

Mycorrhizal fungi strain efficiently growing with rhododendron seedling

A technology of mycorrhizal fungi and strains, applied in the field of microbiology, can solve the problems of affecting nutrient absorption of azalea seedlings, slow growth of azalea seedlings, and high mortality, so as to improve the survival rate of seedlings, increase the infection rate of mycorrhizal plants, and promote seedlings. effect of growth

Active Publication Date: 2012-10-03
SHANGHAI ACADEMY OF LANDSCAPE ARCHITECTURE SCI & PLANNING
View PDF1 Cites 9 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, Rhododendron is an acidic soil plant, and the optimum soil pH value is 4.5~5.5. In the artificial breeding environment, it is difficult to maintain the acidic environment of pH 4.5~5.5 in the cultivation soil. Often the soil pH is too high, which affects Rhododendron seedlings. nutrient absorption
Due to the above two reasons, rhododendron seedlings often grow slowly, with yellow leaves and high mortality, which has become a bottleneck for wild rhododendron hybrid breeding and seedling breeding.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Mycorrhizal fungi strain efficiently growing with rhododendron seedling
  • Mycorrhizal fungi strain efficiently growing with rhododendron seedling
  • Mycorrhizal fungi strain efficiently growing with rhododendron seedling

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] Example 1 Isolation and identification of rhododendron mycorrhizal fungi:

[0014] 1. Separation of root materials: Take the living roots of the rhododendron species ‘Purple Crane’ in Shanghai Binjiang Forest Park, put them in an ice bucket together with soil moisture, and store them in a 4°C refrigerator for later use.

[0015] 2. Separation and identification medium:

[0016] (1) Separation medium: Use Martin-Bengal red medium (referred to as MA) to adjust and improve its pH. The formula is: 10 grams of glucose, 5 grams of tryptone, 1 gram of dipotassium hydrogen phosphate, 0.5 grams of magnesium sulfate, 0.033 grams of Bengal red, 20 grams of agar, distilled water to 1000 ml, adjust the pH value to 5.0, and then 121 ℃ high pressure Sterilize for 20 minutes, add 0.03 g of streptomycin when cooled to below 60°C, mix well, and pour it into a plate for later use.

[0017] (2) Strain identification medium: use malt extract medium (MEA), the formula is: 20g of malt extra...

Embodiment 2

[0032] The strain of the present invention is isolated from the root system of the rhododendron variety 'Purple Crane' in Shanghai Binjiang Forest Park. It is Ascomycetes Oidiodendron (Oidiodendron) OBJF31. It can be cultured on plates and liquids, using MEA medium, and the formula is: malt Extract 20g, tryptone 1g, glucose 20g, suitable culture medium pH value 5.1, culture temperature 22~24℃.

[0033] The macroscopic characteristics of the culture of the strain are as follows: the colony grows slowly, and it is off-white at first. After 10 days, the color of the center of the colony becomes darker and brown. cm. Microscopic characteristics: Mycelia diameter 2.0~3.0μm, with unicellular conidia, oval, smooth surface, size 2.5~3.5μm*1.5~2.0μm, with conidiophores, length 80~120μm. According to the ITS sequence comparison of the strain, the closest species is the fungus of the genus Oidiodendron, which is consistent with the identification results of the morphological characteris...

Embodiment 3

[0038] Seedlings of Rhododendron yunnanensis cultured for 9 weeks in aseptic culture, with a height of about 2-4 cm, were transplanted in substrates with different pH values ​​that were sterilized. The adjustment method of the pH value of the cultivation medium: based on the original pH value of the cultivation medium, a certain amount of CaCO is added according to the volume ratio of the substrate. 3 (addition ratio according to 0, 3, 5kg / m 3 ), adjust the pH value of the cultivation substrate to 5.5, 6.8, 7.2 respectively. Seedlings were inoculated after transplanting. The inoculated strains were OBJF31 (preservation number: CGMCC No.6010) and HS04. The inoculation method and seedling culture conditions were the same as in Example 1. 30 plants were inoculated for each treatment, and no inoculation controls were set respectively. After 20 weeks of inoculation, the infection rate and biomass of the seedlings were detected (Table 2). The biomass and survival rate of the inocul...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
Diameteraaaaaaaaaa
Diameteraaaaaaaaaa
Login to View More

Abstract

The invention belongs to the field of microbiology and provides a mycorrhizal fungi strain efficiently growing with a rhododendron seedling. The problems of the prior art that the seedling growth is slow, the death rate is high, the leaves are yellow, and the like, during the artificial seedling culturing process for the rhododendron are solved. The preservation number of the mycorrhizal fungi strain provided by the invention is CGMCCNo:6010; the mycorrhizal fungi strain belongs to ascomycetes, chactomium globosum, discellaceae, oidiodendron; the mycorrhizal fungi strain provided by the invention can efficiently grow together with the rhododendron seedling; the rhododendron seedling is inoculated in a seedling stage; the infection rate of mycorrhiza is high; the growth of the seedling is obviously boosted; the survival rate of the seedling is increased; and when the pH of soil is 5.5-7.2, the strain inoculated on the seedling can effectively promote the growth of the seedling.

Description

Technical field: [0001] The invention relates to the field of microbiology, in particular to a fungal strain, in particular to a rhododendron-like mycorrhizal fungal strain, in particular to a mycorrhizal fungal strain that efficiently symbioses with rhododendron seedlings. Background technique: [0002] Rhododendron is one of the top ten traditional famous flowers in my country. It has the reputation of "the beauty of flowers" and has high ornamental value. It has always been loved by people. At the same time, my country is the main distribution center of Rhododendron in the world, with about 70% of Rhododendron species resources distributed in the world, and many precious Rhododendron resources are unique to my country. Rhododendron is an important component of vegetation in many nature reserves, forest tourist attractions or forest parks, forming a characteristic and prominent vegetation landscape, which has important ecological value and tourism development value. Moreo...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N1/14A01N63/04A01P21/00C12R1/645
Inventor 张春英黄芳尹丽娟陈真杨兵
Owner SHANGHAI ACADEMY OF LANDSCAPE ARCHITECTURE SCI & PLANNING
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com