35 results about "Acipenser dabryanus" patented technology

The invention provides a wild-training method for improving the feeding ability of released Acipenser dabryanus. The wild-training method comprises the following steps that fish bait is produced; feeding domestication is conducted, wherein feeding domestication starts when are hatched, feeding domestication is conducted on the Acipenser dabranus which are fed with artificial feed for 2-4 months and feeding domestication is conducted on the Acipenser dabranus which are fed with the artificial feed for over 5 months; Through the wild-training method for improving the feeding ability of the released Acipenser dabrayanus, after training is over, both the feeding capability and the predation capability of the Acipenser dabryanus with different specificications are obviously enhanced and improved. After analog wild-release is conducted, the trained Acipenser dabryanus is released to the wild or an experimental pond, the capability of preying on wild life live baits is obviously improved, and the growth rate and the survival rate are obviously higher than the released Acipenser dabryanus which is not trained.

The invention discloses a torrent fish domesticating method. After first filial generation of acipenser dabryanus of 1-5 ages is raised in a culture pond, and then the torrent fishes to domesticated are added to the pond for raising. According to the domesticating method, the acipenser dabryanus and the torrent fishes are raised together, so that the stress response of wild fishes can be reduced, and the ichthyophthirius multifiliis infection resistance and the large individual characteristics of the acipenser dabryanus are utilized, and torrent is created by fishtailing to drive the activity of the wild fishes; the large cluster of the wild fishes is reduced and the probability of reciprocal infection of the ichthyophthirius multifiliis is reduced; as a result, the ichthyophthirius multifiliis can be effectively prevented, the survival rate is increased, the drug use is reduced and the pollution to the water environment is reduced.

The invention relates to the technical field of aquaculture, in particular to an artificial breeding method for acipenser dabryanus. Oxytocin is injected into female acipenser dabryanus two times, wherein the interval between two times of injection is 12 hours, and the injection amount of the first time accounts for 1 / 3 to 1 / 2 of the total dose. The one-time injection method is adopted for male acipenser dabryanus, wherein injection of the male acipenser dabryanus is synchronous with the first time of injection of the female acipenser dabryanus, and the dose of the male acipenser dabryanus is half that of the female acipenser dabryanus. Fertilization is conducted according to a half-dry method, water, eggs and seminal fluid are mixed according to the proportion of 75:50:1, and the mixture is stirred for 2 minutes to 3 minutes. A complete acipenser dabryanus artificial breeding technique process is provided, and technical support is provided for large-scale artificial breeding; due to the adoption of the method, the hatchability of acipenser dabryanus can reach 85% or higher at most, and restrict factors in the acipenser dabryanus artificial breeding process are eliminated.

The present invention relates to a microstructure slicing technology, and specifically relates to an acipenser dabryanus oosperm tissue slicing method. The acipenser dabryanus oosperm tissue slicing method mainly comprises seven steps of material fixation, dehydration, transparentizing, wax dipping, embedding, slicing and staining; a fixing liquid used in the material fixation is a Bouin's fixing liquid and a formaldehyde fixing liquid with the mass fraction of 10%; and the total dehydration time is 10-20h. The acipenser dabryanus oosperm tissue slicing method aims at sturgeon oosperm which belongs to a viscid egg, has the characteristics of being rich in vitellogenin, tough and dense in egg envelope, narrow in perivitelline space, and the like, a complete serial tissue slicing method suitable for sturgeon oosperm is established, the sturgeon oosperm can be efficiently processed, and better paraffin sections can be made.

The invention discloses a real-time quantitative PCR detection primer set and method for differentially expressed genes in the gonads of Acipenser dabkini. The primer set includes a pair of SOX9 primers, a pair of FST primers, a pair of DF primers or a pair of β-actin primers. The detection method comprises the following steps: extracting the RNA of the sample of Acipenser dabryi to be tested, using the total RNA as a template, synthesizing the cDNA of the sample of Acipenser dabryi to be tested; The primer set for real-time quantitative PCR detection of the gene is used for real-time fluorescent quantitative PCR detection, and the amplified product is detected according to the results of agarose gel electrophoresis and the melting curve of the amplified product. The primers of the real-time quantitative PCR target gene and the internal reference gene and the quantitative PCR detection method of the present invention are not only applicable to Dabry's sturgeon, but also can be used for other sturgeons with similar genus and kinship. research provides a basis.

The invention discloses a method for artificially regulating and realizing the natural mating and spawning of Dabry's sturgeon. For the first time, the present invention adopts artificial regulation technical measures such as B-ultrasound parent non-damage identification, selection of sexually mature fish, riverbed quality regulation, water flow regulation, etc., thereby realizing natural Ovulation, natural ejaculation, and simultaneous male and female maturation. It avoids the traditional use of artificial hormone induction, artificial extrusion of the abdomen for egg collection, artificial insemination, damage to the fish body and artificial selection process, which is a major breakthrough in the protection of Dabry's sturgeon. Utilizing the method provided by the present invention, the fertilization rate of Dabry's sturgeon is comparable to artificial propagation (60-80%), and due to the agglomeration and adhesion of fertilized eggs, the seedling hatching rate (20-50%) is lower than artificial propagation, However, the survival rate (80%-90%) of hatched seedlings is higher than that of artificially propagated seedlings.

The invention relates to the technical field of artificial breeding, in particular to a secondary debonding method for acipenser dabryanus. The method includes the steps of placing fertilized eggs in a container after the fertilized eggs of acipenser dabryanus are hatched to the gap-shaped blastopore period-wide neural plate period and are hatched for 46 hours to 54 hours at the temperature of 18 DEG C, adding talcum powder with the mass concentration of 8% to 12% or milk powder with the mass concentration of 13% to 17%, stirring the mixture in the same direction for 15 minutes to 30 minutes, and placing the fertilized eggs in a sturgeon hatching device directly or after washing the fertilized eggs to continue to be hatched. By means of the method, stability of water flow in the hatching process is ensured, man-made stimulation of the critical period of fertilized egg hatching is reduced, the probability of fertilized egg blocking is greatly lowered, the probability of blocky water molds is lowered, the loss ratio of live eggs is decreased, and the hatchability is improved.