A method for tissue sectioning of fertilized eggs of Acipenser dabryi
A technique for tissue sectioning and sturgeon, applied in the field of microscopic tissue sectioning, can solve the problems of small size, low success rate of sectioning, inability to completely remove the membrane, etc., achieve tough and dense egg membrane, reduce tedious operation steps, and enrich egg yolk material. Effect
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Embodiment 1
[0031] A method for slicing tissues at the cleavage stage of fertilized eggs of Dabry's sturgeon, comprising the following steps:
[0032] Material fixation: Put the eggs of Sturgeon dabryi's that have developed to the cleavage stage of fertilized eggs into 50 times the volume of Bouin's fixative solution for 24 hours, wash them repeatedly with 70% ethanol, and then store them in 70% ethanol every other day Replace the 70% alcohol once for three consecutive times to fully wash off the fixative and save it for future use.
[0033] Dehydration: Gradient dehydration of fertilized eggs stored in 70% ethanol, 80% ethanol dehydration once for 2 hours, 90% ethanol dehydration once for 2 hours, 95% ethanol dehydration twice for 2 hours each time, and 100% ethanol dehydration twice, Each dehydration 1h.
[0034] Transparent: Put the dehydrated fertilized eggs into the transparent transition solution with a volume fraction of terpineol: absolute ethanol = 1:1 for 1 hour, then treat wit...
Embodiment 2
[0040] A method for slicing gastrula stage tissue of the fertilized eggs of Dabry's sturgeon, comprising the following steps:
[0041] Material fixation: fertilized eggs of Acipenser dabryi at different stages were fixed in 10% formaldehyde fixative solution for 24 hours, washed repeatedly with 70% ethanol, and then stored in 70% ethanol every other day Replace the 70% alcohol once for three consecutive times to fully wash off the fixative and save it for future use.
[0042] Dehydration: Gradient dehydration of fertilized eggs stored in 70% ethanol, 80% ethanol dehydration once for 2 hours, 90% ethanol dehydration once for 2 hours, 95% ethanol dehydration twice for 2 hours each time, and 100% ethanol dehydration twice, Each dehydration 1h.
[0043] Transparent: Put the dehydrated fertilized eggs into the transparent transition solution with volume fraction terpineol: absolute ethanol = 1:1 for 2 hours, then treat with terpineol for the first time for 4 hours, and for the sec...
Embodiment 3
[0049] A method for tissue slicing at the fertilized egg stage of Dabry's sturgeon zygote, comprising the following steps:
[0050] Material fixation: fertilized eggs of Acipenser dabryi at different stages were fixed in 50 times the volume of Bouin's fixative solution for 24 hours, washed repeatedly with 70% ethanol, and then stored in 70% ethanol, replaced every other day 70% alcohol, three times in a row to fully wash off the fixative, and save for future use.
[0051] Dehydration: Gradient dehydration of fertilized eggs stored in 70% ethanol, 80% ethanol dehydration once for 2 hours, 90% ethanol dehydration once for 2 hours, 95% ethanol dehydration twice, each time for 1 hour, and then 100% ethanol dehydration twice, Each dehydration 1h.
[0052] Transparent: Put the dehydrated fertilized eggs into the transparent transition solution with a volume fraction of terpineol: absolute ethanol = 1:1 for 2 hours, and then treat them with terpineol for 5 hours for the first time a...
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