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Sturgeon egg preservation balance solution

A technology for balancing liquid and eggs, applied in the preservation, application, animal husbandry and other directions of human or animal body, can solve the problem of different time of oxytocin effect of artificial breeding sturgeon male and female, and achieve the effect of expanding the scope of application and overcoming different biochemical compositions.

Inactive Publication Date: 2019-02-01
CHINESE STURGEON RES INST CHINA THREE GOR
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In order to solve the above-mentioned technical problems, the present invention provides a balance solution for the preservation of sturgeon eggs, which realizes the short-term preservation of the eggs of Chinese sturgeon, Sturgeon's sturgeon and Dabry's sturgeon, and solves the technology brought about by the difference in time of induced labor between male and female sturgeon in artificial reproduction. problem

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Preparation of balance solution

[0020] The basic balance solution includes: glucose 1.0g / L, HEPES buffer salt 0.95g / L, NaH 2 PO 4 0.41g / L, KCl0.38g / L, MgSO 4 0.23g / L, NaCl 7.25g / L, CaCl 2 0.147g / L, penicillin: 200,000 IU / L, streptomycin sulfate: 0.2g / L, pH 7.0, body cavity fluid pH 7.36, storage temperature 16°C, storage time 2 hours. The results are shown in Table 1:

[0021] Table 1

[0022]

[0023] The preservation effect is very unsatisfactory. It is guessed that there is no protein in the basic balance solution. BSA0.8g / L, 1.0g / L, and 1.2g / L were added to the balance solution for experiment 2. The pH of the preservation solution was 7.0, and the pH of the body cavity fluid was 7.0. is 7.44, the storage temperature is 16°C, and the storage time is 2 hours and 6 hours, respectively. The results are shown in Table 2 below:

[0024] Table 2

[0025]

[0026] From the results in Table 2, it can be seen that the viability of eggs close to that of fres...

Embodiment 2

[0043] A balance liquid for preserving sturgeon ovum, said balance liquid comprising:

[0044] NaCl: 5-8g / L, HEPES buffer salt: 0.92-0.98g / L, NaH 2 PO 4 : 0.35-0.45g / L, glucose: 0.8-1.2g / L, KCl: 0.35-0.42g / L, bovine serum albumin BSA: 0.5-1.6g / L, CaCl 2 : 0.141-0.153g / L, Penicillin: 180,000-220,000 IU / L, MgSO 4 : 0.19-0.28g / L, streptomycin sulfate: 0.15-0.25g / L, adjust the pH value to be the same as the body cavity fluid.

Embodiment 3

[0046] A balance liquid for preserving sturgeon ovum, said balance liquid comprising:

[0047] NaCl: 5g / L, HEPES buffer salt: 0.92g / L, NaH 2 PO 4 : 0.35g / L, glucose: 0.8g / L, KCl: 0.35g / L, bovine serum albumin BSA: 0.5g / L, CaCl 2 : 0.141g / L, Penicillin: 180,000 IU / L, MgSO 4 : 0.19g / L, streptomycin sulfate: 0.15g / L, adjust the pH value to be the same as the body cavity fluid.

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Abstract

The invention relates to a sturgeon egg preservation balance solution, wherein the balance solution comprises: 1.0 g / L of glucose, 0.95 g / L of HEPES buffer salt, 0.8-1.2 g / L of bovine serum albumin BSA, 0.41g / L of NaH2PO4, 0.38g / L of KCl, 0.23g / L of MgSO4, 7.25g / L of NaCl, 0.147g / L of CaCl2, 200,000 IU / L of penicillin, and 0. 2g / L of streptomycin sulfate, wherein the pH value is adjusted to be thesame as the body cavity fluid. The balance solution of the invention can realize the short-term preservation of the egg of Chinese sturgeon, the egg of acipenser schrenckii, and the egg of acipenserdabryanus, the storage temperature is 16 DEG C, after 6 hours of storage, the fertilization rate of Chinese sturgeon egg is 77.35%, the hatching rate is 80. 06%, the abnormal rate is 0, the fertilization rate of acipenser schrenckii egg is 89.97%, the hatching rate is 91.24%, the abnormal rate is 0, the fertilization rate of acipenser dabryanus egg is 90.03%, the hatching rate is 89.45%, and the abnormal rate is 0. 47%, so that the solution can be used to solve the technical problems caused by the unsynchronized spawning induction effect time of artificial breeding of male and female sturgeon.

Description

technical field [0001] The invention belongs to the field of preparation and application of a short-term preservation balance liquid for aquatic organism eggs, and relates to a balance liquid for preservation of sturgeon eggs. Background technique [0002] After being excreted, fish eggs will become overripe due to morphological and biochemical changes, which will have a negative impact on the viability of the eggs. Therefore, how to prolong the viability of fish eggs is becoming more and more important in aquaculture. important. Sturgeons often have the problem of asynchrony between female spawning and male sperm production during the process of artificial induction. How to maintain the vitality of eggs in a short period of time, as well as a high fertilization rate and hatching rate, is a problem that must be solved in artificial breeding of sturgeons. Practical problems. Various factors such as temperature, light, oxygen, pH, and preservation solution will affect the ef...

Claims

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Application Information

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IPC IPC(8): A01N1/02
CPCA01N1/021A01N1/0226
Inventor 谭春杜合军刘雪清胡亚成刘娟娟
Owner CHINESE STURGEON RES INST CHINA THREE GOR
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