126 results about "Streptomycin Sulfate" patented technology

The invention provides a frozen semen diluent formula for flocks and herds, and the formula of each 100mL of diluent comprises the following components: 1.35-1.55g of citric acid, 2.4-2.6g of trihydroxymethyl aminomethane (tris), 1.0-1.2g of glucose, 4.5-6.8ml of glycerol, 18.0-21ml of yolk, 4.5-7.5ml of bovine serum albumin (BSA), 0.45-0.75ml of vitamin B12, 50000-100000IU of benzylpenicillin potassium, 50000-100000IU of streptomycin sulfate and the balance of distilled water. The formula provided by the invention is low in cost, simple and convenient in preparation and using and operation steps, capable of effectively improving the sperm motility, the acrosome integrity rate and the plasmalemma integrity rate, and capable of reducing the sperm aberration rate.

The invention discloses an anti-freezing agent and an anti-freezing diluent for freezing and storing livestock sperm and a preparation method thereof. The anti-freezing agent for freezing and storing bovine and porcine sperm is algin, the amount of the algin added into the conventional diluent is 60 to 110 milligrams, wherein the conventional diluent is prepared from 1.1 grams of glucose, 1.48 grams of citric acid, 2.42 grams of Tris, 10 percent of fresh egg yolk, 0.06 gram of penicillin sodium and 0.1 gram of streptomycin sulfate, which are dissolved in 100 millimeters of distilled water. The adding amount of the algin for cattle is 100 to 110 milligrams, and the adding amount of the algin for pigs is 60 to 80 milligrams. The preparation method comprises the following steps of: adding 100 to 110 milligrams of algin and 4 percent glycerin (for cattle) or 60 to 80 milligrams of algin and 1 percent glycerin (for pigs) into the conventional diluent to prepare the anti-freezing diluent; and adjusting the pH value of the solution to be between 6.5 and 7.5, filtering and sterilizing the anti-freezing diluent under the osmotic pressure of 286mOsm / L, and putting the anti-freezing diluent into a 4 DEG C refrigerator for later use. The algin is used for freezing and storage of the bovine and porcine sperm for the first time, and after unfreezing, the sperm motility rate, the acrosome completeness rate and the plasma membrane completeness rate are 65 percent, 69 percent and 72 percent (for cattle) respectively and 58 percent, 62 percent and 63 percent (for pigs) respectively.

The medicine ointment for treating burns and scalds is prepared with rhubarb, bletilla tuber, garden burnet, clove, frankincense and other nine kinds of Chinese medicinal materials, as well as streptomycin sulfate and medicinal vaseline in certain weight proportion. The Chinese medicinal materials have the functions of eliminating heat toxin, resisting bacteria, dissipating blood clots, eliminating swelling, relieving pain, etc., so that the medicine ointment of the present invention compounded based on Chinese medicine theory has the advantages of fast wound healing, elimination of skin grafting operation, less pain, no scar and pigmentation, no skin adhesion, etc.

A method for culturing the non-seed crossed Oumei grape includes such steps as preparing the solution from streptomycin sulfate, gibberellin and distilled water, immersing the bunches of flower in the solution from the 6 days before blossoming to the 3 days after blossoming, preparing the solution of gibberellin or forchlorfenuron, and treating the grapes with the solution about 14 days after blossoming. Its non-seed ratio can reach more than 95%.

The invention belongs to the technical field of pig genetic breeding, and specifically relates to a culture solution capable of improving in vitro maturation rate of pig oocytes and application thereof. The culture solution capable of improving the in vitro maturation rate of pig oocytes is prepared from the following raw materials with the following concentrations: 9.5 g / L of TCM-199, 2.2 mg / mL of NaHCO3, 3.05 mmol / L of D-glucose, 0.91 mmol / L of Sodium Pyruvate, 0.5 g / L of Streptomycin sulfate, 0.05% of PVA, 0.57 mmol / L of Cysteine and 0.075 g / L of Penicillin G. The 0.05% of PVA and 5% of a follicular fluid are added on the basis of pig oocyte in vitro maturation culture solution widely used in our country, so that the in vitro maturation rate of the pig oocytes can be improved to more than 90%, and matured oocytes with higher quality and more quantity can be provided for porcine somatic cell nuclear transfer technology.

The invention discloses spore suspension for improving the yield of white muscardine silkworms and application of the spore suspension. The spore suspension for improving the yield of the white muscardine silkworms comprises the following raw materials: muscardine spore powder with 1x106-5x106 muscardine spores per mL, 0.2 part by mass of polysorbate-80, 1-3 parts by mass of cane sugar, 0.4-0.6 part by mass of peptone, 0.05 part of auxiliary materials and the balanced sterile water. The auxiliary materials can be magnesium sulfate, chloramphenicol and streptomycin sulfate. By the spore suspension for improving the yield of the white muscardine silkworms, the concentration of the optimized muscardine suspension is increased, the stickiness and the activity of the muscardine suspension are improved, the germination rate of the muscardine suspension is increased, and the yield and the quality of the white muscardine silkworms are improved obviously.

The invention discloses an antifreeze agent and a diluent for livestock semen refrigeration preservation and application thereof. The antifreeze agent for livestock semen refrigeration preservation is betaine. The diluent for livestock semen refrigeration preservation includes a conventional diluent and an antifreeze agent, the antifreeze agent is betaine, and the conventional diluent formula is preferred as follows: 1.1g of glucose, 1.48g of citric acid, 2.42g of Tris, 10% of fresh egg yolk, 0.06g of penicillin sodium, 0.1g of streptomycin sulfate and 100ml of distilled water. The antifreeze agent for livestock semen refrigeration preservation is especially suitable for artificial insemination of cattle, pigs, sheep tubule frozen semen, and semen deposition after long-term storage and long-distance transportation of super-low temperature frozen semen, sperm motility rate, perforatorium integrity rate and plasmalemma integrity rate after freezing-thawing can be significantly improved, and the sperm fertilization vitality can be maintained.

A cell cryopreservation liquid for human umbilical cord mesenchymal stem cells is disclosed. Components of the liquid include 41.8-51.8% of a serum-free culture solution, 40-50% of a serum substitute,8% of dimethyl sulfoxide, 0.1% of streptomycin sulfate having a concentration of 200 [mu]g / mL and 0.1% of an additive. A cryopreservation method utilizing the cell cryopreservation liquid for the human umbilical cord mesenchymal stem cells is further provided. The method includes centrifuging suspension cells after subculture, then putting the cells into a centrifuge tube, adding the cryopreservation liquid into the centrifuge tube containing the cells, transferring the mixture to a cryopreservation tube, putting the cryopreservation tube requiring cryopreservation into a programmed cooling box containing isopropanol, allowing the cryopreservation tube to stay overnight at -80 DEG C and then transferring the cryopreservation tube to a liquid nitrogen tank the next day. A problem that fetal calf serum in conventional cryopreservation liquids brings about animal-sourced components and pathogen contamination can be solved. The added streptomycin sulfate can reduce the content of antibiotics, reducing human umbilical cord mesenchymal stem cell poisoning caused by antibiotics, reducing cell death and maintaining characteristics of the stem cells.

The invention discloses anti-oxidative cattle frozen semen diluent. The anti-oxidative cattle frozen semen diluent is composed of the following raw materials by mass: 20-26 parts of trihydroxy methyl-aminomethane, 10-15 parts of trisodium citrate, 7-15 parts of fructose, 700-800 parts of distilled water, 230-260 parts of fresh yolk, 0.125-0.375 part of sodium selenite, 2-32 parts of Vitamin E, 90-95 parts of glycerol, 1-2 parts of penicillin potassium for injection (specification of 1.6 million units / g) and 1-2 parts of streptomycin sulfate for injection (specification of 1.0 million units / g). The preparation method of the anti-oxidative cattle frozen semen diluent comprises the steps of preparing a basic buffer solution, a basic diluent and a semen diluent; then transferring the prepared semen diluent to a condition of 4 DEG C, and balancing for 5-20 min to obtain the anti-oxidative cattle frozen semen diluent. Compared with a conventional anti-oxidative cattle frozen semen diluent, the anti-oxidative cattle frozen semen diluent provided by the invention increases semen motility rate by 24.56%, increases 4 h semen motility rate by 12.39%, increases semen perforatorium integrity rate by 38.72%, increases GSH-PX activity by 260%, and reduces semen aberration rate by 5.49%.

The invention discloses a compound composition containing methanesulphonyl myclobutanil and an antibiotics bactericide and a preparation. The compound composition consists of the methanesulphonyl myclobutanil and the antibiotics bactericide, wherein the weight ratio of the methanesulphonyl myclobutanil to the antibiotics bactericide is (100:1)-(1:30), and preferably (30:1)-(1:10); the antibiotics bactericide is selected from any one of kasugamycin, streptomycin sulfate, validamycin, polyoxin, tetramycin, blasticidin, antifungalmycine, ningnanmycin, cytosintetidemycin and oxytetracycline hydrochloride. The compound composition and the preparation provided by the invention have a remarkable synergistic interaction effect in comparison with single dosage, and are capable of effectively controlling the bacterial diseases and / or fungal diseases such as cucumber bacterial angular leaf spot, tobacco bacterial wilt and rice blast so as to improve the control effect, reduce the dosage of the pesticides, reduce the control cost and be more widely to sterilize.

The invention provides a nano-gold-based method for visually and rapidly detecting antibiotics in milk. The method disclosed by the invention is mainly characterized in that pyrocatechol violet is used for reducing chloroauric acid to prepare the nano-gold. In a nano-gold synthesizing process, the addition of the antibiotics (including kanamycin mono-sulfate, neomycin sulfate, streptomycin sulfate and bleomycin sulfate) influences the synthesis of the nano-gold so that the color is changed; and the antibiotics can be quantitatively detected through naked eyes and an ultraviolet visible absorption spectrometer. According to the method, the operation is simple and rapid, the sensitivity is high, the selectivity is good, and the method can be applied to the detection of the antibiotics in the milk.

The invention discloses 3-substituted-1-methyl-quinazoline-2,4-dione compounds shown in formula 1, a preparation method and application of the 3-substituted-1-methyl-quinazoline-2,4-dione compounds. Through in-vitro anti-microbial activity test, it is found that these compounds have certain inhibitory activity on gram-positive bacteria (Staphylococcus aureus, methicillin-resistant Staphylococcus aureus, Bacillus subtilis), gram-negative bacteria (Escherichia coli, proteusbacillus vulgaris, Pseudomonas aeruginosa) and fungus (Nostoc, Candida Albicans). Inhibitory activities of partial compounds on partial tested strains are approximate to or even superior to those of existing drugs such as streptomycin sulfate and polyoxin D. The compounds are especially sensitive to methicillin-resistant Staphylococcus aureus, can be prepared into antibacterial and / or anti-fungal drugs, and have the advantages of simple preparation method, easily-accessible materials and low cost.

The invention discloses an artificial feed of proxenus lepigone larva. According to part by weight, the feed comprises the following constituents of 200-220 parts of corn starch, 118-125 parts of soybean meal, 40-43 parts of yeast powder, 27-29 parts of sugar, 3.5-4 parts of sorbic acid, 28-30 parts of agar, 40-42 parts of casein, 10-12 parts of composite vitamin, 2-3.5 parts of vitamin C, 0.1-0.2 part of streptomycin sulfate, 2.5-5 parts of methanol, 4-6 parts of alcohol, 1-3 parts of vegetable oil and 1000-1500 parts of sterilized distilled water. The artificial feed of proxenus lepigone larva, disclosed by the invention, is available in raw material and low in cost, is convenient to prepare, can be stored for long time, has comprehensive nutritional ingredient, stable feed effect, highpracticability and obvious economical benefit, and is favorable for raising the proxenus lepigone larva on a large scale.

The invention discloses a method for reducing explant pollution in tea tree tissue culture process. The treatment method comprises the following steps: sterilizing a selected explant, cutting off the discolored cut part of the explant due to sterilization, cutting the part to a 1-1.5 cm long stem section with an axillary bud, inoculating the axillary bud on a culture medium containing antibiotic, inoculating 1-2 stem sections in each bottle, and placing the stem sections in a culture room for culture after being inoculated. The method disclosed by the invention combines ampicillin with streptomycin sulfate to reduce the pollution rate of the explant in the tea tree tissue culture process so as to improve the survival rate.

The invention discloses a special medicine fertilizer for konjak. The special medicine fertilizer is characterized by being formed by the following components in mass percentage: 15-27% of a base material and 73-85% of a medicine fertilizer. The medicine fertilizer is formed by the following raw materials in parts by weight: 16-21 parts of urea, 22-28 parts of monoammonium phosphate, 34-36 parts of potassium sulfate, 0.08-0.1 part of nitryl fulvic acid salt, 0.06-0.08 part of agricultural streptomycin sulfate and 0.1-0.12 part of chlorothalonil. When the special medicine fertilizer is used, 50-80kg of the medicine fertilizer is applied to each 666.7 m<2>. The medicine fertilizer disclosed by the invention can improve the anti-disease capability of a konjak plant and prevents main konjak disease bacteria from invading konjak plants and corms; the medicine fertilizer ensures the sufficient requirements of the growth of the konjak on the a main fertilizer; and the medicine and the fertilizer are combined so that the special medicine fertilizer prevents diseases, balances nutrition, improves the soil, improves the medicine fertilizer effects, enhances the konjak resistance, accelerates the growth of the konjak and has the very obvious social, economic and ecological comprehensive benefits.

The invention discloses a streptomycin sulfate detection method. The method is implemented through a specific reaction of a chemical fluorescent probe with streptomycin sulfate and comprises steps as follows: 1) dissolving the chemical fluorescent probe in an organic solvent to obtain a solution A, and dissolving alkali in water to obtain a solution B; 2) dissolving the streptomycin sulfate in water to prepare a series of streptomycin sulfate aqueous solutions with different concentrations, that is, standard solutions; 3) mixing and oscillating the solution A and the solution B with the standard solutions with the different concentrations respectively, leaving the mixtures to stand for layering, transferring water layers, measuring the fluorescence intensity, and establishing a correlation curve about the fluorescence intensity and the concentrations of the standard solutions; 4) mixing and oscillating the solution A and the solution B with a to-be-detected sample solution, leaving the mixture to stand for layering, transferring a water layer, measuring the fluorescence intensity, comparing the curve with the curve established in Step 3), and calculating the content of the streptomycin sulfate in a sample.

The invention discloses a preparation method of an anti-disease medicine against potato bacterial wilt, which comprises the following steps: (A) material preparation: preparing streptomycin sulfate, 25% cuaminosulfate aqueous solution, 2% formalin, glycyrrhizic acid, chlorogenic acid, polyoxin, radix ampelopsis and dried orange peel; (B) alcohol digestion of the extraction liquid of radix ampelopsis and dried orange peel: crushing the radix ampelopsis and dried orange peel, washing and airing; putting into a closed container, adding 70% ethanol solution by mass, and soaking for 12 hours; putting into a percolator, wherein the percolator is of a bucket structure with a cone at the bottom, the cone is downward connected with equipment which can be opened / closed to drop water, and the percolator is used for extracting the components of the medicinal materials; uniformly putting the medicinal materials into the bucket, and adding the soaking liquid water or wine slowly and dropwise; collecting the percolation liquid; and heating to recover ethanol to obtain the extraction liquid; and (C) compounding the streptomycin sulfate, 25% cuaminosulfate aqueous solution, 2% formalin, glycyrrhizic acid, chlorogenic acid and polyoxin with the extraction liquid obtained by the step (B), stirring uniformly and performing ultraviolet sterilization to obtain the anti-disease medicine against potato bacterial wilt. The preparation method disclosed by the invention is simple, and the prepared product is simple in formula and convenient to use and realizes a good effect in treating the potato bacterial wilt.

The invention belongs to the technical field of organisms and discloses a method for removing explant endophyte during plant tissue culturing. The cuttage soil can be disinfected by 50% of carbendazim, cuttage twigs are disinfected by sterile water solution containing 200 mg / L of amphotericin and 100 mg / L of streptomycin sulfate, and new semi-lignified twigs can be treated in advance by sterile water solution containing 100 mg / L of amphotericin and 50 mg / L of streptomycin sulfate. Accordingly, bacterial and fungi on the surface of and inside the explant such as aspen can be effectively removed, and sufficient vigor of the explant can be kept.

The invention discloses a secondary metabolite of gingko endophytic fungus Nigrospora sphaerica. The metabolite is 9 alpha-hydroxyl dihydrodeoxyspiroxin. The 9 alpha-hydroxyl dihydrodeoxyspirotrichinenot only has an inhibiting effect on ralstonia solanacearum, but also has a good inhibiting effect on common typical gram-negative food-borne pathogenic bacteria escherichia coli, typical gram-positive food-borne pathogenic bacteria staphylococcus aureus and bacillus subtilis, the effect of the 9 alpha-hydroxyl dihydrodeoxyspirotrichine is equivalent to that of antibiotic streptomycin sulfate, and the metabolite has important application in the aspects of agricultural biocontrol and food preservation.

The invention discloses a using method of a potato bacterial wilt-resistant drug. The using method comprises the following steps: A, preparing a stock solution comprising the following components: streptomycin sulfate, a 25% cuaminosulfate aqueous solution, a 2% formalin solution, licorice acid, chlorogenic acid, polyoxin, radix ampelopsis and dried orange peel; B, cultivating potatoes: selecting disease-free seed potatoes, soaking the seed potatoes for 1.5-2 hours by using 400-fold stock solution in a volume ratio, taking out and rinsing the seed potatoes with clear water, and planting, wherein when the seed potatoes are cut into blocks, a cutter is soaked and sterilized by 75% alcohol; C, in case of the bacterial wilt, adding water to dilute the stock solution to 600 folds for spraying potato plants; D, adding water to dilute the stock solution to 500 folds for irrigating roots at the same time of spraying in the step C. The using method is simple; used components are simple in mixture ratio; the using method is convenient; through the adoption of the method, a good effect of treating the bacterial wilt of the potatoes can be achieved.

The invention relates to application of ZnO nanomaterial in inhibiting activity of Erwinia carotovora subsp. Carotovora. The application includes using ZnO independently, or mixing ZnO and streptomycin sulfate and ultrasonically treating for 30 min; adding into a suspension containing Erwinia carotovora subsp. Carotovora; and acting for 2 hr, to inhibit Erwinia carotovora subsp. Carotovora. Compared with prior arts, the application has the advantages of easily accessible materials, low cost, simple operation, simple equipment, and suitability for mass production.

The invention relates to a sheep seminal fluid diluent, and a preservation method. Each 100ml of the sheep seminal fluid diluent comprises Y-27632 2HCl 0.16 to 3.20mg, vitamin E 3.5 to 4.5ml, fresh yolk 19 to 21ml, fructose 1.2 to 1.3g, citric acid monohydrate 1.7 to 1.8g, trimethylolaminomethane 3.5 to 3.6g, vitamin C 0.4 to 0.6 g, bovine serum albumin 0.4 to 0.6 g, penicillin sodium 10000 to 15000 IU, streptomycin sulfate 1000 to 15000 IU, glycerin 3.5 to 4.5ml, and the balance double distilled water. The sheep seminal fluid diluent is capable of prolonging preservation time of sheep seminalfluid under low temperature conditions, and increasing the quality of preserved sperm.

The invention discloses a surface plasmon resonance sensor chip for detecting gram-negative bacteria and a preparation method and application thereof. The chip comprises a glass substrate layer, a gold film layer and a probe molecular layer. The gold film layer is disposed on the glass substrate layer. The probe molecular layer is disposed on the gold film layer. A probe molecule of the probe molecule layer contains at least one of drug molecules of streptomycin sulfate, kanamycin sulfate, gentamicin sulfate, gentamycin-micronomicin, vistamycin sulfate, neomycin, polymyxin B, rifampicin, chloramphenicol and fosfomycin. The invention also discloses a preparation method and application of the chip. The surface plasmon resonance sensor chip creatively realizes detection of gram-negative bacteria based on surface plasmon resonance, has high sensitivity and good selectivity, has a gram-negative bacterium concentration detection linear range of 10-10<6> CFU / mL and can be used for detecting gram-negative bacteria in solutions such as detection water, drinking water or human body fluids.

The invention relates to a method for establishing liver cell lines of semilabeo obscurus. The method includes steps of 1), acquiring the livers of the semilabeo obscurus: disinfecting the bodies of the semilabeo obscurus by methylene blue and alcohol jointly; 2), carrying out primary culture: carrying out culture on the livers by the aid of high-glucose DMEM (dulbecco modified eagle medium) culture fluid with kanamycin sulfate and streptomycin sulfate; 3), carrying out passage culture: replacing the cell culture fluid by basic culture fluid when the sixth generation of livers is subjected to passage culture, and successfully establishing the liver cell lines of the semilabeo obscurus. The method has the advantages that the obtained liver cell lines of the semilabeo obscurus are irregularly polygonal, can be subjected to continuous culture transfer and can be directly applied to research on biological characteristics, and requirements on conservation of resources of semilabeo obscurus species and theoretical research and application can be met.

The invention belongs to the field of biology and particularly relates to a method applicable to in-vitro mature culture of young rabbit oocytes and a formulation of a culture medium. According to the method, a cumulus-oocytes complex is cultured in vitro by using a TCM-199 basic culture system; based on a TCM-199 culture medium, 10% of FBS, 0.11 g / L of sodium pyruvate, 10 Mug / ml of LH, 10 Mug / ml of FSH, 1 Mug / ml of E2, 100 IU / ml of penicillin, 100 IU / ml of streptomycin sulfate and 15-20 Mum of epigallocatechin gallate (EGCG). According to the invention, the EGCG is added into the TCM-199 culture medium to study the influence of the EGCG on in-vitro maturation of young New Zealand rabbit oocytes, so that a simple and practical method for in-vitro maturation of the young rabbit oocytes is established.

The invention relates to a preparation method of a diluent for storing sheep semen at a normal temperature. The preparation method comprises the following steps: 1, dissolving 30.2 g to 31.2 g of Tris, 15.9 g to 16.9 g of citric acid, 19.5 g to 20.5 g of fructose, 0.3 g to 0.4 g of penicillin sodium and 0.6 g to 0.7 g of streptomycin sulfate into 1000mL of sterilized ultrapure water to obtain a basic diluent, 2, when 95 mL of the basic diluent is taken, adding 5 mL of yolk into 95 mL of the basic diluent so that a first diluent is obtained, when 85mL of the basic diluent is taken, adding 15mLof yolk into the 85mL of the basic diluent to obtain a second diluent, when 80 mL of the basic diluent is taken, adding 20 mL of yolk into 80 mL of the basic diluent so that a third diluent is obtained, putting the first diluent, the second diluent and the third diluent into a refrigerator to be fully dissolved, then centrifuging for 20 minutes at the speed of 10,000 r / min and the temperature of 4DEG C, and taking out a supernatant after centrifuging, so as to obtain a yolk diluent with the concentration of 5%, a yolk diluent with the concentration of 15% and a yolk diluent with the concentration of 20%. Through the diluent provided by the invention, the semen preservation effect is improved.