Anti-oxidative cattle frozen semen diluent and preparation method thereof
A technology for freezing semen and diluent, which is applied in the preservation, application, animal husbandry and other directions of human or animal body, and can solve the problem of insufficient antioxidant damage ability, reduce the survival rate, survival rate and acrosome integrity rate, and reduce the quality of semen, etc. question
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Embodiment 1
[0045] Antioxidant bovine frozen semen diluent, composed of the following raw materials in parts by mass: 20 parts of trishydroxymethylaminomethane, 10 parts of trisodium citrate, 7 parts of fructose, 700 parts of distilled water, 230 parts of fresh egg yolk, sodium selenite 0.125 parts, 2 parts of vitamin E, 90 parts of glycerin, 1 part of penicillin potassium for injection (1.6 million units / g), 1 part of streptomycin sulfate for injection (1 million units / g).
[0046] The steps for preparing the antioxidant bovine frozen semen dilution are as follows:
[0047] (1) Preparation of basic buffer solution Take 20 parts of Tris, 10 parts of trisodium citrate, and 7 parts of fructose, mix well, add 700 parts of distilled water and stir well, set aside:
[0048] (2) Prepare the basic dilution solution Take 230 parts of fresh egg yolk and mix evenly with the basic buffer solution prepared in (1), then add 1 part of penicillin potassium for injection (specification: 1.6 million units...
Embodiment 2
[0052] Antioxidant bovine frozen semen diluent, composed of the following raw materials in parts by mass: 26 parts of trishydroxymethylaminomethane, 15 parts of trisodium citrate, 15 parts of fructose, 800 parts of distilled water, 260 parts of fresh egg yolk, sodium selenite 0.375 parts, 32 parts of vitamin E, 95 parts of glycerin, 2 parts of penicillin potassium for injection (1.6 million units / g), 2 parts of streptomycin sulfate for injection (1 million units / g).
[0053] The steps for preparing the antioxidant bovine frozen semen dilution are as follows:
[0054] (1) Preparation of basic buffer solution Take 26 parts of Tris, 15 parts of trisodium citrate, and 15 parts of fructose, mix well, add 800 parts of distilled water and stir well, set aside:
[0055] (2) Prepare the basic dilution solution Take 260 parts of fresh egg yolk and mix evenly with the basic buffer solution prepared in (1), then add 2 parts of penicillin potassium for injection (1.6 million units / g) and 2...
Embodiment 3
[0059] Antioxidant bovine frozen semen diluent, composed of the following raw materials in parts by mass: 24 parts of trishydroxymethylaminomethane, 12 parts of trisodium citrate, 10 parts of fructose, 750 parts of distilled water, 240 parts of fresh egg yolk, sodium selenite 0.25 parts, 15 parts of vitamin E, 92 parts of glycerin, 1.5 parts of penicillin potassium for injection (1.6 million units / g), 1.5 parts of streptomycin sulfate for injection (1 million units / g).
[0060] The steps for preparing the antioxidant bovine frozen semen dilution are as follows:
[0061] (1) Preparation of basic buffer solution Take 24 parts of Tris, 12 parts of trisodium citrate, and 10 parts of fructose, mix well, add 750 parts of distilled water and stir well, and set aside:
[0062] (2) Prepare the basic dilution solution Take 240 parts of fresh egg yolk and mix evenly with the basic buffer solution prepared in (1), then add 1.5 parts of penicillin potassium for injection (1.6 million units...
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