Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Anti-oxidative cattle frozen semen diluent and preparation method thereof

A technology for freezing semen and diluent, which is applied in the preservation, application, animal husbandry and other directions of human or animal body, and can solve the problem of insufficient antioxidant damage ability, reduce the survival rate, survival rate and acrosome integrity rate, and reduce the quality of semen, etc. question

Inactive Publication Date: 2014-09-03
山西省生态畜牧产业管理站
View PDF4 Cites 9 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Artificial insemination is a mature technology, but when using frozen semen for artificial insemination, its fertilization effect is lower than that of using fresh semen. Quality change is an important factor affecting the success of artificial insemination
[0004] The existing frozen semen dilutions are usually added with diluents, nutrients, antibacterial, antifreeze, anti-shock and buffer protection agents, etc., while the substances that can resist oxidative damage during the freezing and thawing of semen are limited to vitamins. E, vitamin C and glutathione and other substances, due to insufficient anti-oxidative damage ability, reduce the survival rate, survival rate and acrosome integrity rate after thawing, and reduce the quality of semen

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Antioxidant bovine frozen semen diluent, composed of the following raw materials in parts by mass: 20 parts of trishydroxymethylaminomethane, 10 parts of trisodium citrate, 7 parts of fructose, 700 parts of distilled water, 230 parts of fresh egg yolk, sodium selenite 0.125 parts, 2 parts of vitamin E, 90 parts of glycerin, 1 part of penicillin potassium for injection (1.6 million units / g), 1 part of streptomycin sulfate for injection (1 million units / g).

[0046] The steps for preparing the antioxidant bovine frozen semen dilution are as follows:

[0047] (1) Preparation of basic buffer solution Take 20 parts of Tris, 10 parts of trisodium citrate, and 7 parts of fructose, mix well, add 700 parts of distilled water and stir well, set aside:

[0048] (2) Prepare the basic dilution solution Take 230 parts of fresh egg yolk and mix evenly with the basic buffer solution prepared in (1), then add 1 part of penicillin potassium for injection (specification: 1.6 million units...

Embodiment 2

[0052] Antioxidant bovine frozen semen diluent, composed of the following raw materials in parts by mass: 26 parts of trishydroxymethylaminomethane, 15 parts of trisodium citrate, 15 parts of fructose, 800 parts of distilled water, 260 parts of fresh egg yolk, sodium selenite 0.375 parts, 32 parts of vitamin E, 95 parts of glycerin, 2 parts of penicillin potassium for injection (1.6 million units / g), 2 parts of streptomycin sulfate for injection (1 million units / g).

[0053] The steps for preparing the antioxidant bovine frozen semen dilution are as follows:

[0054] (1) Preparation of basic buffer solution Take 26 parts of Tris, 15 parts of trisodium citrate, and 15 parts of fructose, mix well, add 800 parts of distilled water and stir well, set aside:

[0055] (2) Prepare the basic dilution solution Take 260 parts of fresh egg yolk and mix evenly with the basic buffer solution prepared in (1), then add 2 parts of penicillin potassium for injection (1.6 million units / g) and 2...

Embodiment 3

[0059] Antioxidant bovine frozen semen diluent, composed of the following raw materials in parts by mass: 24 parts of trishydroxymethylaminomethane, 12 parts of trisodium citrate, 10 parts of fructose, 750 parts of distilled water, 240 parts of fresh egg yolk, sodium selenite 0.25 parts, 15 parts of vitamin E, 92 parts of glycerin, 1.5 parts of penicillin potassium for injection (1.6 million units / g), 1.5 parts of streptomycin sulfate for injection (1 million units / g).

[0060] The steps for preparing the antioxidant bovine frozen semen dilution are as follows:

[0061] (1) Preparation of basic buffer solution Take 24 parts of Tris, 12 parts of trisodium citrate, and 10 parts of fructose, mix well, add 750 parts of distilled water and stir well, and set aside:

[0062] (2) Prepare the basic dilution solution Take 240 parts of fresh egg yolk and mix evenly with the basic buffer solution prepared in (1), then add 1.5 parts of penicillin potassium for injection (1.6 million units...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses anti-oxidative cattle frozen semen diluent. The anti-oxidative cattle frozen semen diluent is composed of the following raw materials by mass: 20-26 parts of trihydroxy methyl-aminomethane, 10-15 parts of trisodium citrate, 7-15 parts of fructose, 700-800 parts of distilled water, 230-260 parts of fresh yolk, 0.125-0.375 part of sodium selenite, 2-32 parts of Vitamin E, 90-95 parts of glycerol, 1-2 parts of penicillin potassium for injection (specification of 1.6 million units / g) and 1-2 parts of streptomycin sulfate for injection (specification of 1.0 million units / g). The preparation method of the anti-oxidative cattle frozen semen diluent comprises the steps of preparing a basic buffer solution, a basic diluent and a semen diluent; then transferring the prepared semen diluent to a condition of 4 DEG C, and balancing for 5-20 min to obtain the anti-oxidative cattle frozen semen diluent. Compared with a conventional anti-oxidative cattle frozen semen diluent, the anti-oxidative cattle frozen semen diluent provided by the invention increases semen motility rate by 24.56%, increases 4 h semen motility rate by 12.39%, increases semen perforatorium integrity rate by 38.72%, increases GSH-PX activity by 260%, and reduces semen aberration rate by 5.49%.

Description

technical field [0001] The invention belongs to the production technology of animal frozen semen, and in particular relates to a preparation method of frozen semen diluent added with trace elements selenium and vitamin E. Background technique [0002] The reproduction of cattle is an important link in the production process of cattle breeding. The increase in the number and quality of cattle must be realized through the process of reproduction. Artificial insemination and embryo transfer technology are two major technological revolutions in the production of cattle breeding, which can give full play to the reproductive potential of excellent breeding bulls and cows, showing a good application prospect. The premise of artificial insemination and embryo transfer technology is the application of bovine semen preservation technology. The success of cryopreservation of semen is a major innovation in artificial insemination technology. It not only solves the problem of long-term ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): A01N1/02
Inventor 白元生张拴林杨子森刘晓妮师周戈赵宇琼
Owner 山西省生态畜牧产业管理站
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products