34 results about "Potassium penicillin g" patented technology

The invention discloses anti-oxidative cattle frozen semen diluent. The anti-oxidative cattle frozen semen diluent is composed of the following raw materials by mass: 20-26 parts of trihydroxy methyl-aminomethane, 10-15 parts of trisodium citrate, 7-15 parts of fructose, 700-800 parts of distilled water, 230-260 parts of fresh yolk, 0.125-0.375 part of sodium selenite, 2-32 parts of Vitamin E, 90-95 parts of glycerol, 1-2 parts of penicillin potassium for injection (specification of 1.6 million units / g) and 1-2 parts of streptomycin sulfate for injection (specification of 1.0 million units / g). The preparation method of the anti-oxidative cattle frozen semen diluent comprises the steps of preparing a basic buffer solution, a basic diluent and a semen diluent; then transferring the prepared semen diluent to a condition of 4 DEG C, and balancing for 5-20 min to obtain the anti-oxidative cattle frozen semen diluent. Compared with a conventional anti-oxidative cattle frozen semen diluent, the anti-oxidative cattle frozen semen diluent provided by the invention increases semen motility rate by 24.56%, increases 4 h semen motility rate by 12.39%, increases semen perforatorium integrity rate by 38.72%, increases GSH-PX activity by 260%, and reduces semen aberration rate by 5.49%.

The invention provides a method for synthesizing penicillin G sulfoxide by using a continuous flow reactor, and the method comprises the following steps: (1) providing the continuous flow reactor which comprises a peroxidation reaction system and an oxidation reaction system; (2) respectively putting hydrogen peroxide and glacial acetic acid into a first containing bottle and a second containing bottle in the peroxidation reaction system, and enabling the hydrogen peroxide to be in contact with the glacial acetic acid so as to obtain peracetic acid; and (3) enabling the peracetic acid to enterthe oxidation reaction system through a pre-cooling module, and contacting the peracetic acid with a penicillin G potassium salt aqueous solution so as to obtain the penicillin G sulfoxide and a penicillin G sulfoxide aqueous solution. The method can be used for effectively synthesizing the penicillin G sulfoxide, and in the whole process of the method, reaction materials are easy to obtain, treatment after reaction is simple, industrial three wastes are easy to treat, the yield of a target product is high, and the method is very suitable for industrial production.

The invention discloses a penicillin V potassium micro-capsule and a preparation method thereof. The penicillin V potassium micro-capsule consists of a core material penicillin V potassium and a capsule wall material ethyecellulose. The preparation method comprises the following steps: 1) smashing and dissolving ethyecellulose in dichloromethane to obtain a solution a; 2) dissolving the smashed penicillin V potassium under stirring at a rotating speed of 100-500rpm in the solution a to obtain a solution b, and continuously stirring for 20-30 minutes; 3) controlling temperature of water and dispersing a surfactant in water to obtain a solution c; and 4) under the condition of stirring at a speed of 100-500rpm, adding the solution b into the solution c to form the penicillin V potassium micro-capsule, continuously stirring till dichloromethane is fully volatilized, filtering under reduced pressure, and washing the micro-capsule three times by using distilled water, and drying to obtain the penicillin V potassium micro-capsule. According to the invention, the micro-capsule is prepared by a drying-in-liquid method to eliminate harmful smell of penicillin V potassium so as to improve the compliance of the patient. The preparation process of the micro-capsule uses easily available and low-price raw materials, is low in production cost, simple in process operation, easy to operate and implement and high in yield.

The invention discloses bacillus tequilensis Am101 capable of degrading various beta-lactam antibiotics and an application of the bacillus tequilensis Am101. The strain is preserved in China General Microbiological Culture Collection Center on November 18, 2019, and a preservation number is CGMCC NO. 18965. The strain Am101 disclosed by the invention can be used for simultaneously degrading various beta-lactam antibiotics and can be used for efficiently degrading benzylpenicillin potassium, amoxicillin, cefuroxime sodium and ceftiofur sodium. The strain Am101 can be applied to degradation treatment of residual beta-lactam antibiotics in livestock and poultry manure, can also be applied to restoration of water and soil polluted by the beta-lactam antibiotics, and has the wide application prospect.

The invention relates to a method for recovering phenylacetic acid from a benzylpenicillin potassium lysis solution and belongs to the technical field of pharmacy. The method for recovering the phenylacetic acid from the benzylpenicillin potassium lysis solution comprises the steps that the benzylpenicillin potassium lysis solution is extracted through an organic solvent; an organic phase is decolored through an activated carbon and alkalized through a sodium hydroxide solution; and the obtained alkalized water phase is oxidized with hydrogen peroxide and decolored with activated carbon so that a sodium phenylacetate aqueous solution with a light color can be obtained, the sodium phenylacetate aqueous solution is acidized and crystallized through concentrated sulfuric acid, washed with cold water and dried, and phenylacetic acid is obtained. The obtained phenylacetic acid product is high in quality, no waste difficult to deal with is generated in the recovering process, and the method is suitable for large-scale application.

The invention provides a penicillin V acylase mutant, an encoding array, a recombinant expression vector, a genetically engineered bacterium and application. Penicillin V acylase derived from Bacillussphaericus is mutated, the specific activity of the obtained mutant PVA-3 is improved by nine times, the immobilization activity is improved to 820U / g from 92U / g, and after being incubated for one hour under a condition of 30% penicillin V sylvite, the mutant has activity residue of 89.1%; the reaction time that the immobilized mutant PVA-3 is adopted to prepare 6-APA (6-Aminopenicillanic Acid) by splitting 25% penicillin V under a condition that the pH value is 6.0 at 25 DEG C is shortened to 60 minutes from 180 minutes, a substrate conversion rate is increased to 99.5% or greater, the activity is not greatly lost after continuous use for 600 times or greater, and good operation stability can be achieved. By adopting the mutated PVA-3, the production cost can be greatly reduced, the production efficiency can be improved, and the mutant is applicable to industrial application.

The present invention is directed to a kit for evaluating on the skin of a patient the sensitivity to penicillin, comprising (A) a first vial containing a major determinant mixture, the major determinant mixture comprising benzylpenicilloyl polylysine; (B) a second vial containing lyophilized minor determinant mixture, the minor determinant mixture comprising a lyophilized mixture of neutralized (1) penicillin G potassium; (2) penicilloic acid; and (3) penilloic acid; (C) a third vial containing amoxicillin sodium; and (D) instructions for carrying out a method to evaluate on the skin of a patient the sensitivity to penicillin. The present invention is also directed to a method to rule out penicillin allergy using the disclosed kit.

The invention provides penicillin V potassium tablets and a preparation technology thereof. The penicillin V potassium tablets are prepared from, by weight, 267 parts of penicillin V potassium, 8-10 parts of povidone, 3-5 parts of talc powder and 2-4 parts of magnesium stearate. The preparation technology comprises the steps of granulating of penicillin V potassium through the povindone solution, drying, grain size stabilizing, total blending, tabletting and film coating. According to the penicillin V potassium tablets, the stability is good, the disintegration time meets the specification, and little effective substance content is decreased in the expiry date; the production cost and interference on detection are reduced while the variety and dosage of auxiliary materials are reduced. The penicillin V potassium tablets obtained through the preparation method have the advantages that the tablets are not burst, a small tablet weight difference is generated, and no sticking occurs in the tabletting process.

The invention provides a preparation method of 6-APA (6-amino-penicillanic acid). 6-APA is prepared from a potassium carbonate reversed-extraction liquid of benzylpenicillin potassium directly, a molecular formula of 6-APA is C8H12N2O3S, and the molecular weight is 216.26. With the adoption of the process, a crystallization step can be omitted, and crystallization yield loss is avoided; extracting and washing processes of a penicillin solvent are optimized in a technical process; with the adoption of the advanced preparation process, the preparation method is higher in yield and lower in cost.

The invention discloses a preparation method of 7-ADCA. The method includes the steps that penicillin G potassium salt serves as a raw material, hydrogen peroxide serves as an oxidizing agent, liquid BSU serves as a protective agent, a combination of pyridine hydrobromide and oxalic acid serves as a ring enlargement catalyst, and 7-ADCA is obtained through the steps of oxidization, ring enlargement, enzymolysis and the like. The method has the advantages that safety of the production process is high, production cost is low, emission of three wastes is small, operation steps are fewer, the yield is high, and quality is good.

The invention belongs to the technical field of chemistry and chemical engineering, and particularly relates to a method for preparing penicillin sulfoxide ester through continuous flow, which comprises the following steps: mixing hydrogen peroxide and a catalyst solution to prepare an oxidant, and carrying out continuous flow oxidation reaction on the oxidant and penicillin G potassium ester, and carrying out continuous flow quenching reaction on the oxidation reaction liquid by using a sodium hydrogen sulfite aqueous solution, and carrying out post-treatment to obtain the penicillin sulfoxide ester (GESO). According to the present invention, the method has advantages of simple operation, improved safety of the reaction unit, sustainability, high unit area production capacity compared with the traditional intermittent reaction mode, environmental protection and the like, the conversion rate of the preparation method can achieve 100%, the selectivity can achieve 99%, the separation yield can achieve 96%, and the method is suitable for industrial production. The content is greater than 99.5%, and the single impurity content index reaches the original research process standard.

The invention discloses the endophytic fungus Letendraea sp. WZ07 and its application in nano-silver synthesis. The fungal strain was isolated from the invasive plant Trichosanthes marinum, and was deposited in the China Center for Type Culture Collection on August 4, 2020, with the preservation number CCTCC NO: M2020395. The strain was identified by the ITS sequence, which had the highest similarity (100%) to Letendraea sp.1 NV-2015, and was identified as Letendraea sp.WZ07, and the GenBank accession number of the ITS gene sequence of the strain was MN712504. The fungal strain can grow in the potato dextrose medium containing streptomycin sulfate and penicillin potassium, and its optimum temperature is 28°C and optimum pH is 5. Strain Letendraea sp.WZ07 has the ability to synthesize nanosilver and catalyze AgNO 3 The solution synthesizes nano-silver with different particle sizes and shapes. This strain expands the application of fungi in the green synthesis of nanomaterials, and also provides a new microbial resource for the biological preparation of silver nanomaterials.

The invention discloses a synthesis method of penicillin G sulfoxide benzhydryl ester. Using penicillin G potassium salt as the starting material, first esterification, and then 2KHSO 5 ·KHSO 4 ·K 2 SO 4 The oxidative synthesis of penicillin G sulfoxide benzhydryl ester has a product purity of more than 99% and a total molar yield of more than 95%, and is suitable for industrial production. The invention has the advantages of readily available raw materials, high yield, less pollution, high safety factor, easy operation and the like.

The invention discloses an intermediate isomer for preparing cefovecin by taking penicillin potassium salt as a raw material and a preparation method therefor. An intermediate ring-opening rearrangement material is used as a raw material to perform isomerization reaction to obtain the intermediate isomer; the method specifically comprises the following steps: adding a dehydrated reaction organic solvent into a reaction container, then adding intermediate oxides, regulating temperature to 90-100 DEG C, stirring until the solution is clear, adding trimethyl phosphite, adjusting the vacuum degree, controlling the reaction temperature to be 90-100 DEG C, reacting until the reaction is complete, cooling to 30 DEG C or lower under the protection of nitrogen, adding organic alkali into the reaction container, and reacting in a dark place at the temperature of 30 DEG C or below under the protection of nitrogen until the reaction is complete to obtain the intermediate isomer. Compared with theprior art, when the intermediate is synthesized, a one-pot method is adopted for preparation, and the operation difficulty is reduced. Because the stability of the cephalosporin mother nucleus is poorer, the reaction selectivity is improved by strictly controlling the reaction conditions, and finally, the reaction yield is high and the product quality is good.

A rapid method for quickly identifying penicillin potassium (sodium) for injection which is converted into penicillin acid with white deposit when combined with acid, and the white deposit disappears through the molecule synthesis by shaking rapidly. And it is a quick and accurate detecting method for penicillin potassium (sodium) for injection by non-professional person in non- lab circumstance .

The invention discloses a penicillin V potassium micro-capsule and a preparation method thereof. The penicillin V potassium micro-capsule consists of a core material penicillin V potassium and a capsule wall material ethyecellulose. The preparation method comprises the following steps: 1) smashing and dissolving ethyecellulose in dichloromethane to obtain a solution a; 2) dissolving the smashed penicillin V potassium under stirring at a rotating speed of 100-500rpm in the solution a to obtain a solution b, and continuously stirring for 20-30 minutes; 3) controlling temperature of water and dispersing a surfactant in water to obtain a solution c; and 4) under the condition of stirring at a speed of 100-500rpm, adding the solution b into the solution c to form the penicillin V potassium micro-capsule, continuously stirring till dichloromethane is fully volatilized, filtering under reduced pressure, and washing the micro-capsule three times by using distilled water, and drying to obtain the penicillin V potassium micro-capsule. According to the invention, the micro-capsule is prepared by a drying-in-liquid method to eliminate harmful smell of penicillin V potassium so as to improve the compliance of the patient. The preparation process of the micro-capsule uses easily available and low-price raw materials, is low in production cost, simple in process operation, easy to operate and implement and high in yield.

The invention discloses a Pseudomonas bacterium capable of degrading penicillin V potassium. The Pseudomonas bacterium is preserved in the General Microorganism Center of China Microbiological Culture Collection Management Committee with the preservation number CGMCC No. 15259. The pseudopallidum strain capable of degrading penicillin V potassium of the present invention can use penicillin V potassium as the sole carbon source for growth, and the degradation ability of the bacterial strain to a content of 0.6 g / L penicillin V potassium can reach 100% within 3 hours. The bacterial strain can be used for harmless treatment of fermented bacteria residues in the industrial production of penicillin V potassium. The treatment method is highly efficient, green and environmentally friendly, saves resources, has low treatment cost, and eliminates the adverse impact of penicillin V potassium waste mycelia on the environment.

The invention relates to a method for recovering phenylacetic acid from a benzylpenicillin potassium lysis solution and belongs to the technical field of pharmacy. The method for recovering the phenylacetic acid from the benzylpenicillin potassium lysis solution comprises the steps that the benzylpenicillin potassium lysis solution is extracted through an organic solvent; an organic phase is decolored through an activated carbon and alkalized through a sodium hydroxide solution; and the obtained alkalized water phase is oxidized with hydrogen peroxide and decolored with activated carbon so that a sodium phenylacetate aqueous solution with a light color can be obtained, the sodium phenylacetate aqueous solution is acidized and crystallized through concentrated sulfuric acid, washed with cold water and dried, and phenylacetic acid is obtained. The obtained phenylacetic acid product is high in quality, no waste difficult to deal with is generated in the recovering process, and the method is suitable for large-scale application.

The invention discloses a culture medium for quickly detecting the population quantity of isaria cateinannulata in soil. The culture medium comprises the following raw materials in parts by weight of40 parts of glucose, 10 parts of peptone, 18 parts of agar powder, 0.1g / L streptomycin sulfate, 0.1g / L potassium benzylpenicillin, 1 part of CuSO4-5H2O powder and 1000ml of water. The culture medium prepared from the glucose, the peptone and the agar powder provides nutrient substances and ample energy sources of a carbon source, a nitrogen source, growth factors and the like for the isaria cateinannulata; through the killing effects of dual antibiotics (streptomycin sulfate and potassium benzylpenicillin) on bacteria, the tolerance of the isaria cateinannulata on Cu2+ in the CuSO4-5H2O powder, and restraining of the Cu2+ to non-objective fungi, the capacity of the culture medium for resisting infectious microbes is high, the separation efficiency of an objective fungus namely the isaria cateinannulata reaches 100%, the shortcoming of being large in deviation of detected data caused by interference of the infectious microbes is avoided, the population quantity of the isaria cateinannulata in soil can be quickly detected, the isaria cateinannulata can be accurately applied to fields, and the preventing and controlling effects of pests can be increased.

The invention provides penicillin V potassium tablets and a preparation technology thereof. The penicillin V potassium tablets are prepared from, by weight, 267 parts of penicillin V potassium, 8-10 parts of povidone, 3-5 parts of talc powder and 2-4 parts of magnesium stearate. The preparation technology comprises the steps of granulating of penicillin V potassium through the povindone solution, drying, grain size stabilizing, total blending, tabletting and film coating. According to the penicillin V potassium tablets, the stability is good, the disintegration time meets the specification, and little effective substance content is decreased in the expiry date; the production cost and interference on detection are reduced while the variety and dosage of auxiliary materials are reduced. The penicillin V potassium tablets obtained through the preparation method have the advantages that the tablets are not burst, a small tablet weight difference is generated, and no sticking occurs in the tabletting process.

The invention provides a penicillin V acylase mutant, an encoding array, a recombinant expression vector, a genetically engineered bacterium and application. Penicillin V acylase derived from Bacillussphaericus is mutated, the specific activity of the obtained mutant PVA-3 is improved by nine times, the immobilization activity is improved to 820U / g from 92U / g, and after being incubated for one hour under a condition of 30% penicillin V sylvite, the mutant has activity residue of 89.1%; the reaction time that the immobilized mutant PVA-3 is adopted to prepare 6-APA (6-Aminopenicillanic Acid) by splitting 25% penicillin V under a condition that the pH value is 6.0 at 25 DEG C is shortened to 60 minutes from 180 minutes, a substrate conversion rate is increased to 99.5% or greater, the activity is not greatly lost after continuous use for 600 times or greater, and good operation stability can be achieved. By adopting the mutated PVA-3, the production cost can be greatly reduced, the production efficiency can be improved, and the mutant is applicable to industrial application.

The invention discloses a strain of Bacillus tequila Am101 capable of degrading various β-lactam antibiotics and its application. The strain was deposited in the General Microorganism Center of China Committee for Microorganism Culture Collection on November 18, 2019, with the preservation number CGMCC NO.18965. The bacterial strain Am101 of the present invention can simultaneously degrade multiple β-lactam antibiotics, and can efficiently degrade penicillin potassium, amoxicillin, cefuroxime sodium and ceftiofur sodium. Strain Am101 can be applied to the degradation treatment of residual β-lactam antibiotics in livestock and poultry manure, and can also be used to restore water and soil polluted by β-lactam antibiotics, with broad application prospects.

The invention provides a preparation method of high-purity penicillin potassium reference substance, which comprises the steps of dissolving the penicillin potassium intermediate in purified water at 5±1°C, adding an organic solvent at 5±1°C, and keeping the prepared mixed solution at 5°C. ±1°C. While stirring, slowly add an organic solvent, crystallize, filter with suction, wash and dry. The reference substance prepared by the method has high purity, high content, high light transmittance, good crystal form and convenient weighing.

The invention discloses a Pseudochrobactrum strain capable of degrading penicillin V potassium. The Pseudochrobactrum strain is preserved in the China General Microbiological Culture Collection Center(CGMCC) with the preservation number of CGMCC No. 15259. The Pseudochrobactrum strain capable of degrading the penicillin V potassium can grow by utilizing the penicillin V potassium as a unique carbon source, and the degradation capacity of the strain to the penicillin V potassium with the content of 0.6g / L can reach 100% within 3 hours. The strain can be used for harmless treatment of fermentation bacterial residues in industrial production of the penicillin V potassium, the treatment method is efficient, green and environment-friendly, resources are saved, the treatment cost is low, and the adverse effect of waste hyphae of the penicillin V potassium on the environment is eliminated.

The invention belongs to the field of synthesis of cephalosporin drug intermediates and disclosesa preparation method of 7-phenylacetamide-3-chloromethyl-cephalosporanic acid p-methoxybenzyl ester (short for GCLE). According to the method, penicillin G potassium salt taken as an initial raw material reacts with 4-methoxybenzylchloride firstly, penicillin sulfoxide ester is prepared through oxidation with peracetic acid and reacts with 2-mercaptobenzothiazole firstly, then a product reacts with benzene sulfinic acid, a ring opening product is obtained and reacts with chlorine, sodium methoxide is used for ring closing under the action of a methanol solvent, and 7-phenylacetamide-3-chloromethyl-cephalosporanic acid p-methoxybenzyl ester is obtained. The solvent is simple and easy to recover, reaction conditions are mild, operation is simple and convenient, and industrial production is easy.

The invention pertains to a novel preparation method of p-nitrobenzyl-7-phenoxy-ace-tamide-3-exomethylenecepham-4-carboxylate-1-Beta-oxide. Penicillin V potassium salt and peracetic acid react to obtain penicillin V sulfoxide by reverse titration; the penicillin V sulfoxide and p-monobromo-methylbenzeneare nitrate are treated with reflux reaction under the action of triethylamine in acetone solution to prepare penicillin V sulfoxide p-nitrobenzyl ester group; the penicillin V sulfoxide p-nitrobenzyl ester group under the action of N-chlorosuccinimide and calcium oxide opens five ring and closes hexatomic ring again under the action of stannic chloride and then reacts with methanol to prepare the p-nitrobenzyl-7-phenoxy-ace-tamide-3-exomethylenecepham-4-carboxylate- 1-Beta-oxide. The preparation method has the advantages of short preparation and reaction time and production cycles, low cost of raw materials, less dosage and high yield.

The present invention provides a kind of penicillin V potassium high-yielding bacterium and its application in fermentative production of penicillin V potassium, the penicillin V potassium high-yielding bacterium is Penicillium chrysogenum ( Penicillium Chrysogenum ) B181126 strain, the preservation date is October 14, 2019, the preservation number is CCTCC NO: M2019823, and the preservation unit is the China Center for Type Culture Collection (CCTCC). When Penicillium chrysogenum B181126 is used in production, with the optimal fermentation process, its fermentation unit can reach more than 80,000 U / mL, which is 36.4% higher than the starting strain, and the fermentation unit of penicillin V potassium is greatly increased. At the same time, 4 ‑ Hydroxypenicillin V potassium impurity content is less than 1.2%.

The invention relates to a method for preparing ampicillin from penicillin potassium, which belongs to the technical field of pharmacy. The method for preparing ampicillin by penicillin potassium according to the present invention, penicillin potassium is cracked into 6-APA and phenylacetic acid under the action of penicillin acylase, and the lysate is extracted with dichloromethane, so that 6-APA is separated from phenylacetic acid . Extract the aqueous phase and add ammonia water, adjust the pH of the feed liquid to neutral, remove methylene chloride, and carry out nanofiltration and concentration of the obtained feed liquid, and the effect of the concentrated liquid and D-phenylglycine methyl ester hydrochloride on amoxicillin synthase Synthesis of ampicillin. It overcomes the inhibitory effect of phenylacetic acid remaining in the 6-APA solution obtained after extraction from the lysate on the enzyme activity of the synthetase. The concentration of 6-APA in the 6-APA solution is high, and there are few colored impurities in the extraction process. The quality of ampicillin products is easy to be applied in production.

The invention discloses a preparation method of 7-ADCA. The method includes the steps that penicillin G potassium salt serves as a raw material, hydrogen peroxide serves as an oxidizing agent, liquid BSU serves as a protective agent, a combination of pyridine hydrobromide and oxalic acid serves as a ring enlargement catalyst, and 7-ADCA is obtained through the steps of oxidization, ring enlargement, enzymolysis and the like. The method has the advantages that safety of the production process is high, production cost is low, emission of three wastes is small, operation steps are fewer, the yield is high, and quality is good.

The invention relates to a degrading bacterial strain PGB1 capable of degrading β-lactam antibiotics and an application thereof, belonging to the technical field of microorganisms. The strain was deposited on September 27, 2017 in the General Microbiology Center of the China Committee for the Collection of Microbial Cultures, and the preservation number is CGMCC NO.14760. The bacterial strain PGB1 of the present invention has a better degradation effect on β-lactam antibiotics, and has a degradation rate of 100.00% for penicillin potassium as the only carbon source and a concentration of 50-300 mg / L, and can be degraded and removed Inhibition of penicillin potassium against Staphylococcus aureus. The strain can be applied to degrade the relevant β-lactam antibiotics left in the production enterprise waste and the environment.