33 results about "Potassium penicillin g" patented technology

The invention discloses a synthesis method of penicillin G sulfoxide benzhydryl ester. Using penicillin G potassium salt as the starting material, first esterification, and then 2KHSO 5 ·KHSO 4 ·K 2 SO 4 The oxidative synthesis of penicillin G sulfoxide benzhydryl ester has a product purity of more than 99% and a total molar yield of more than 95%, and is suitable for industrial production. The invention has the advantages of readily available raw materials, high yield, less pollution, high safety factor, easy operation and the like.

The invention discloses a penicillin V potassium micro-capsule and a preparation method thereof. The penicillin V potassium micro-capsule consists of a core material penicillin V potassium and a capsule wall material ethyecellulose. The preparation method comprises the following steps: 1) smashing and dissolving ethyecellulose in dichloromethane to obtain a solution a; 2) dissolving the smashed penicillin V potassium under stirring at a rotating speed of 100-500rpm in the solution a to obtain a solution b, and continuously stirring for 20-30 minutes; 3) controlling temperature of water and dispersing a surfactant in water to obtain a solution c; and 4) under the condition of stirring at a speed of 100-500rpm, adding the solution b into the solution c to form the penicillin V potassium micro-capsule, continuously stirring till dichloromethane is fully volatilized, filtering under reduced pressure, and washing the micro-capsule three times by using distilled water, and drying to obtain the penicillin V potassium micro-capsule. According to the invention, the micro-capsule is prepared by a drying-in-liquid method to eliminate harmful smell of penicillin V potassium so as to improve the compliance of the patient. The preparation process of the micro-capsule uses easily available and low-price raw materials, is low in production cost, simple in process operation, easy to operate and implement and high in yield.

The invention discloses a Pseudomonas bacterium capable of degrading penicillin V potassium. The Pseudomonas bacterium is preserved in the General Microorganism Center of China Microbiological Culture Collection Management Committee with the preservation number CGMCC No. 15259. The pseudopallidum strain capable of degrading penicillin V potassium of the present invention can use penicillin V potassium as the sole carbon source for growth, and the degradation ability of the bacterial strain to a content of 0.6 g / L penicillin V potassium can reach 100% within 3 hours. The bacterial strain can be used for harmless treatment of fermented bacteria residues in the industrial production of penicillin V potassium. The treatment method is highly efficient, green and environmentally friendly, saves resources, has low treatment cost, and eliminates the adverse impact of penicillin V potassium waste mycelia on the environment.

The invention relates to a method for recovering phenylacetic acid from a benzylpenicillin potassium lysis solution and belongs to the technical field of pharmacy. The method for recovering the phenylacetic acid from the benzylpenicillin potassium lysis solution comprises the steps that the benzylpenicillin potassium lysis solution is extracted through an organic solvent; an organic phase is decolored through an activated carbon and alkalized through a sodium hydroxide solution; and the obtained alkalized water phase is oxidized with hydrogen peroxide and decolored with activated carbon so that a sodium phenylacetate aqueous solution with a light color can be obtained, the sodium phenylacetate aqueous solution is acidized and crystallized through concentrated sulfuric acid, washed with cold water and dried, and phenylacetic acid is obtained. The obtained phenylacetic acid product is high in quality, no waste difficult to deal with is generated in the recovering process, and the method is suitable for large-scale application.

The invention provides penicillin V potassium tablets and a preparation technology thereof. The penicillin V potassium tablets are prepared from, by weight, 267 parts of penicillin V potassium, 8-10 parts of povidone, 3-5 parts of talc powder and 2-4 parts of magnesium stearate. The preparation technology comprises the steps of granulating of penicillin V potassium through the povindone solution, drying, grain size stabilizing, total blending, tabletting and film coating. According to the penicillin V potassium tablets, the stability is good, the disintegration time meets the specification, and little effective substance content is decreased in the expiry date; the production cost and interference on detection are reduced while the variety and dosage of auxiliary materials are reduced. The penicillin V potassium tablets obtained through the preparation method have the advantages that the tablets are not burst, a small tablet weight difference is generated, and no sticking occurs in the tabletting process.

The invention provides a penicillin V acylase mutant, an encoding array, a recombinant expression vector, a genetically engineered bacterium and application. Penicillin V acylase derived from Bacillussphaericus is mutated, the specific activity of the obtained mutant PVA-3 is improved by nine times, the immobilization activity is improved to 820U / g from 92U / g, and after being incubated for one hour under a condition of 30% penicillin V sylvite, the mutant has activity residue of 89.1%; the reaction time that the immobilized mutant PVA-3 is adopted to prepare 6-APA (6-Aminopenicillanic Acid) by splitting 25% penicillin V under a condition that the pH value is 6.0 at 25 DEG C is shortened to 60 minutes from 180 minutes, a substrate conversion rate is increased to 99.5% or greater, the activity is not greatly lost after continuous use for 600 times or greater, and good operation stability can be achieved. By adopting the mutated PVA-3, the production cost can be greatly reduced, the production efficiency can be improved, and the mutant is applicable to industrial application.

The invention discloses a strain of Bacillus tequila Am101 capable of degrading various β-lactam antibiotics and its application. The strain was deposited in the General Microorganism Center of China Committee for Microorganism Culture Collection on November 18, 2019, with the preservation number CGMCC NO.18965. The bacterial strain Am101 of the present invention can simultaneously degrade multiple β-lactam antibiotics, and can efficiently degrade penicillin potassium, amoxicillin, cefuroxime sodium and ceftiofur sodium. Strain Am101 can be applied to the degradation treatment of residual β-lactam antibiotics in livestock and poultry manure, and can also be used to restore water and soil polluted by β-lactam antibiotics, with broad application prospects.

The invention provides a preparation method of high-purity penicillin potassium reference substance, which comprises the steps of dissolving the penicillin potassium intermediate in purified water at 5±1°C, adding an organic solvent at 5±1°C, and keeping the prepared mixed solution at 5°C. ±1°C. While stirring, slowly add an organic solvent, crystallize, filter with suction, wash and dry. The reference substance prepared by the method has high purity, high content, high light transmittance, good crystal form and convenient weighing.

The invention relates to a method for preparing ampicillin from penicillin potassium, which belongs to the technical field of pharmacy. The method for preparing ampicillin by penicillin potassium according to the present invention, penicillin potassium is cracked into 6-APA and phenylacetic acid under the action of penicillin acylase, and the lysate is extracted with dichloromethane, so that 6-APA is separated from phenylacetic acid . Extract the aqueous phase and add ammonia water, adjust the pH of the feed liquid to neutral, remove methylene chloride, and carry out nanofiltration and concentration of the obtained feed liquid, and the effect of the concentrated liquid and D-phenylglycine methyl ester hydrochloride on amoxicillin synthase Synthesis of ampicillin. It overcomes the inhibitory effect of phenylacetic acid remaining in the 6-APA solution obtained after extraction from the lysate on the enzyme activity of the synthetase. The concentration of 6-APA in the 6-APA solution is high, and there are few colored impurities in the extraction process. The quality of ampicillin products is easy to be applied in production.

The invention discloses a preparation method of 7-ADCA. The method includes the steps that penicillin G potassium salt serves as a raw material, hydrogen peroxide serves as an oxidizing agent, liquid BSU serves as a protective agent, a combination of pyridine hydrobromide and oxalic acid serves as a ring enlargement catalyst, and 7-ADCA is obtained through the steps of oxidization, ring enlargement, enzymolysis and the like. The method has the advantages that safety of the production process is high, production cost is low, emission of three wastes is small, operation steps are fewer, the yield is high, and quality is good.

The invention and a method for preparing amoxicillin and phenylacetic acid from penicillin potassium belong to the technical field of pharmacy. According to the method of the present invention, penicillin potassium is cleaved into 6-APA and phenylacetic acid under the action of penicillin acylase, and the lysate is extracted with dichloromethane, so that 6-APA and phenylacetic acid are separated. Extract the aqueous phase and add ammonia water, adjust the pH of the feed solution to neutral, remove methylene chloride, and carry out nanofiltration and concentration of the obtained feed solution, and the concentrated solution and D-hydroxyphenylglycine methyl ester are under the action of amoxicillin synthetase Synthetic amoxicillin. The dichloromethane obtained from the extraction of the lysate is alkalized to obtain an aqueous sodium phenylacetate solution, which is oxidized, decolorized, crystallized, suction filtered, washed and dried to obtain phenylacetic acid. There are few colored impurities in the lysate, the yield of amoxicillin is high, the product quality is good, and the phenylacetic acid with qualified product quality is recovered at the same time, which is easy to be applied in production.