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Strain Am101 capable of degrading various beta-lactam antibiotics and application of strain Am101

A technology of lactams and antibiotics, applied in the field of microorganisms, achieves the effects of wide degradation spectrum, convenient use and simple process

Active Publication Date: 2020-02-14
BEIJING INSTITUTE OF TECHNOLOGYGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are few reported strains that can rapidly and efficiently degrade β-lactam antibiotics, especially those that degrade multiple β-lactam antibiotics at the same time

Method used

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  • Strain Am101 capable of degrading various beta-lactam antibiotics and application of strain Am101
  • Strain Am101 capable of degrading various beta-lactam antibiotics and application of strain Am101
  • Strain Am101 capable of degrading various beta-lactam antibiotics and application of strain Am101

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] 1. Screening of β-lactam antibiotic-degrading strain Am101

[0021] 1. Material preparation

[0022] The source of bacteria samples: fresh pig manure and chicken manure from a livestock and poultry breeding enterprise.

[0023] Antibiotics: penicillin potassium, amoxicillin, cefuroxime sodium, ceftiofur sodium.

[0024] Beef extract peptone medium: 5.0g beef extract, 10.0g peptone, 5.0g NaCl, distilled water, pH7.4-7.6, sterilized at 121°C for 15min. Add 10g-15g of agar to the solid medium.

[0025] Medium No. Ⅱ: beef extract 1.5g, peptone 6.0g, yeast extract powder 6.0g, glucose 1.0g, water 1L, sterilized at 115°C for 20min. Add 10g-15g of agar to the solid medium.

[0026] Inorganic salt medium: (NH 4 ) 2 NO 4 2.0g, K 2 HPO 4 0.5g, KH 2 PO 4 0.5g, MgSO 4 ·7H 2 O 0.5g, NaCl 0.2g, CaCl 2 0.1g, MnSO 4 h 2 O 0.01g, FeSO 4 ·7H 2 O 0.01g, distilled water 1L, sterilized at 121°C for 15-20min. Add 10g-15g of agar to the solid medium.

[0027] Martin's ...

Embodiment 2

[0046] Growth and degradation characteristics of strain Am101

[0047] 1. Growth characteristics of strain Am101

[0048] The strain Am101 was inoculated in different solid media such as beef extract peptone medium, Martin's medium, potato medium, and inorganic salt medium. Potassium, amoxicillin, cefuroxime sodium, and ceftiofur sodium can also grow in the beef extract peptone medium, and the cultivation is easy.

[0049] The suitable culture conditions of the strain Am101 were studied by single factor test, the settings included temperature 35°C-55°C, pH value 5-9, concentration of β-lactam antibiotics 50mg / L-150mg / L, culture time 3-7 Today, it was found that the strain Am101 can grow at 55°C, and can grow at a pH value of 5-9 and a concentration of β-lactam antibiotics of 50mg / L-150mg / L.

[0050] Inoculate the strain Am101 in the beef extract peptone liquid medium, place it at 28--30°C, shake it at 150-180r / min, take samples regularly, measure the OD value at 600nm, and o...

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Abstract

The invention discloses bacillus tequilensis Am101 capable of degrading various beta-lactam antibiotics and an application of the bacillus tequilensis Am101. The strain is preserved in China General Microbiological Culture Collection Center on November 18, 2019, and a preservation number is CGMCC NO. 18965. The strain Am101 disclosed by the invention can be used for simultaneously degrading various beta-lactam antibiotics and can be used for efficiently degrading benzylpenicillin potassium, amoxicillin, cefuroxime sodium and ceftiofur sodium. The strain Am101 can be applied to degradation treatment of residual beta-lactam antibiotics in livestock and poultry manure, can also be applied to restoration of water and soil polluted by the beta-lactam antibiotics, and has the wide application prospect.

Description

technical field [0001] The invention relates to a degrading strain Bacillus tequilensis Am101 capable of degrading multiple β-lactam antibiotics, belonging to the technical field of microorganisms. Background technique [0002] β-lactam antibiotics refer to a large class of antibiotics containing a β-lactam ring in their molecular structure, including penicillins with 6-APA as their nucleus and cephalosporins with 7-ACA as their nucleus. It is widely used in animal husbandry, fishery and clinical because of its broad antibacterial spectrum, strong antibacterial effect and low price. It is the most widely used class of existing antibiotics. [0003] During the fermentation and production of β-lactam antibiotics, a large amount of bacterial residue and waste water will be produced. If they are not properly treated and discharged into the environment, they may remain in soil, water and food; during the use of antibiotics, only a small amount Antibiotics are metabolized by huma...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F3/34B09C1/10A62D3/02C12R1/07C02F101/38A62D101/28
CPCA62D3/02A62D2101/28B09C1/10C02F3/34C02F2101/38C12N1/205C12R2001/07
Inventor 李艳菊王梓诺顾金刚杨正礼张爱萍
Owner BEIJING INSTITUTE OF TECHNOLOGYGY
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