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Culture medium for detecting population quantity of isaria cateinannulata in soil, and preparation method and use method of culture medium

A technology of I. clavulae and culture medium, which is applied in the field of farmland microbial detection, can solve problems such as poor control effect, inability to clearly understand the dynamic changes in the number of I. annulatans, precision application of bacteria, and difficulties in precise prevention and control. It achieves the effects of fast detection speed, easy storage, and strong anti-bacterial ability

Pending Publication Date: 2020-05-22
贵州省烟草公司安顺市公司
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  • Application Information

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Problems solved by technology

[0003] At present, due to the complex and changeable environmental influence on the field control and application of I. annulatans, it is impossible to clearly understand the dynamic changes in the number of I. annucleans in the field, and the control effect is not good; Control brings difficulties

Method used

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  • Culture medium for detecting population quantity of isaria cateinannulata in soil, and preparation method and use method of culture medium
  • Culture medium for detecting population quantity of isaria cateinannulata in soil, and preparation method and use method of culture medium
  • Culture medium for detecting population quantity of isaria cateinannulata in soil, and preparation method and use method of culture medium

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Embodiment Construction

[0026] Hereinafter, preferred embodiments of the present invention will be described in detail with reference to the accompanying drawings. It should be understood that the preferred embodiments are only for illustrating the present invention, but not for limiting the protection scope of the present invention.

[0027] 1. Preparation of culture medium for rapid detection of I. anteroides populations in soil

[0028] Scheme 1: Production of culture medium The raw materials are as follows in parts by weight: Take 40g of glucose, 10g of peptone and 18g of agar powder into a container, add 200mL of water to dissolve, set the volume to 1000ml, keep the pH natural, and put it into a high-pressure sterilization at 121°C Sterilize the pot for 30 minutes. When it is cooled to below 40°C, add 0.1 g / L streptomycin sulfate, 0.1 g / L penicillin potassium, and 1 g of ground CuSO4·5H2O powder. Mix the base thoroughly, pour it into a petri dish before it is hot, and pour the amount to cover t...

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Abstract

The invention discloses a culture medium for quickly detecting the population quantity of isaria cateinannulata in soil. The culture medium comprises the following raw materials in parts by weight of40 parts of glucose, 10 parts of peptone, 18 parts of agar powder, 0.1g / L streptomycin sulfate, 0.1g / L potassium benzylpenicillin, 1 part of CuSO4-5H2O powder and 1000ml of water. The culture medium prepared from the glucose, the peptone and the agar powder provides nutrient substances and ample energy sources of a carbon source, a nitrogen source, growth factors and the like for the isaria cateinannulata; through the killing effects of dual antibiotics (streptomycin sulfate and potassium benzylpenicillin) on bacteria, the tolerance of the isaria cateinannulata on Cu2+ in the CuSO4-5H2O powder, and restraining of the Cu2+ to non-objective fungi, the capacity of the culture medium for resisting infectious microbes is high, the separation efficiency of an objective fungus namely the isaria cateinannulata reaches 100%, the shortcoming of being large in deviation of detected data caused by interference of the infectious microbes is avoided, the population quantity of the isaria cateinannulata in soil can be quickly detected, the isaria cateinannulata can be accurately applied to fields, and the preventing and controlling effects of pests can be increased.

Description

technical field [0001] The invention relates to a culture medium for rapidly detecting the population quantity of I. annulatans in soil and a preparation method thereof, belonging to the field of farmland microorganism detection. Background technique [0002] For a long time, in agricultural production, due to the excessive use of chemical pesticides, serious "3R problems" (Residue residue, Resistance, Resurgence rampant), and green prevention and control technology, because it does not pollute the environment, has strong sustainability and is Many advantages such as human and animal safety are favored by plant protection workers. Among them, the use of entomopathogenic fungi to control agricultural pests has a long history. Among these fungi that have a killing effect on pests, I. annuli is an important entomopathogenic fungus. According to reports, I. annuli widely exists in forests. In ecosystems such as soil and soil, it has been reported that the bacterium can also be ...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/06
CPCC12Q1/06C12Q1/045
Inventor 薛原许忠顺万春华黄锡才李兴华张权王真娣
Owner 贵州省烟草公司安顺市公司
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