Pleurotus eryngii liquid strain with small and many mycelium pellets and fermentation method of pleurotus eryngii liquid strain
A technology of liquid strains and fermentation methods, which is applied in the fields of botany equipment and methods, fungi, mushroom cultivation, etc. It can solve the problems of small diameter of mycelium balls, shorten the age of strains, and the gap in multiplication algebra, and achieve mycelium The effect of small ball diameter, shortening the age of bacteria and the gap between multiplication algebra, and uniform distribution
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Embodiment 1
[0051] Embodiment 1 Raw material preparation
[0052] 1. Preparation of Chinese herbal medicine extract:
[0053] The preparation method of the Chinese herbal medicine extract comprises the steps of: weighing 30 parts of astragalus, 30 parts of dried ginger, 30 parts of tangerine peel, 25 parts of bezoar, 25 parts of paeonol, 20 parts of licorice, and 18 parts of anemarrhena in parts by weight. 18 parts of honeysuckle, 17 parts of Houttuynia cordata, 15 parts of red peony, 15 parts of scutellaria baicalensis, 10 parts of Chuanxiong, and 8 parts of angelica; put them in an ultrasonic cleaner filled with 0.2% sodium bicarbonate solution for 12 minutes at 200W and 30KHz, Drain, crush the above-mentioned Chinese herbal medicine to a particle size of less than 2 mm, put it in a container and mix evenly and add 5 times the weight of water to obtain a mixed material, control the temperature at 80°C for 3 hours, then lower the temperature to 45°C, and adjust the pH value with lactic a...
Embodiment 2
[0061] A method for fermenting liquid strains of Pleurotus eryngii with small and many mycelium balls, comprising the steps of:
[0062] 1) Take the preserved Pleurotus eryngii slant strain 0.2cm 2 Inoculate 2 pieces of the strain block of the above-mentioned bacteria on the plate medium for activation, and culture at a constant temperature of 25°C for 10 days, and activate twice in this way to obtain plate seeds;
[0063] 2) Use a puncher with a diameter of 0.5 cm to intercept the flat seed 0.2 cm 2 3 pieces of the strain block were inserted into a 250mL Erlenmeyer flask, and the amount of liquid seed culture medium was 100mL. They were cultured at a constant temperature of 25°C for 2 days, then placed in a constant temperature shaker, and shaken at 25°C and 100r / min for 3 days. liquid seeds;
[0064] 3) The liquid seeds were inoculated in a 500 mL shake flask with an inoculum amount of 10%, and the liquid volume of the shake flask culture medium was 250 mL, and cultured at...
Embodiment 3
[0075] A method for fermenting liquid strains of Pleurotus eryngii with small and many mycelium balls, comprising the steps of:
[0076] Step 1) to 4) with embodiment 2;
[0077] 5) First inoculate the seeds of the seed tank with 8% inoculation amount in a 30L fermenter, and ferment at 30°C with a ventilation rate of 3L / min for 12 hours at a constant temperature of 15L, and then cool down to 18°C at a rate of 0.15°C / min. ℃, add 13g coated chitosan and 900mL seed tank seeds into the fermenter, ferment at constant temperature for 10h under the condition of ventilation rate 6L / min, and finally raise the temperature to 24℃ at the rate of 0.05℃ / min, Ferment at constant temperature for 45 hours under certain conditions to obtain the liquid strain of Pleurotus eryngii with small and many mycelium balls;
[0078] Described plate medium composition is the same as embodiment 2;
[0079] The liquid seed medium consists of: glucose 30g / L, yeast extract juice 5g / L, peptone 2g / L, agar 2...
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