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Endophytic fungus letendraea sp.WZ07 and its application in the synthesis of nano-silver

An endophytic fungus and nano-silver technology is applied in the field of microorganisms to achieve the effects of simple equipment and low production cost

Active Publication Date: 2022-06-07
HUAQIAO UNIVERSITY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the study on the synthesis of AgNPs by fungi of the genus Letendraea has not been reported so far.

Method used

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  • Endophytic fungus letendraea sp.WZ07 and its application in the synthesis of nano-silver
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  • Endophytic fungus letendraea sp.WZ07 and its application in the synthesis of nano-silver

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Experimental program
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Embodiment 1

[0034] The acquisition of embodiment 1 bacterial strain

[0035] Letendraea sp. WZ07 in this example is isolated from the invasive plant Ageratum conyzoides L. . After washing the stems of the red thistle collected on the campus of Huaqiao University in Xiamen City, Fujian Province with tap water, the stems were cut into 4 cm with a sterile knife in an ultra-clean workbench. The treated stems were subjected to surface disinfection according to the following operation method: soak them in 5% ethanol for 2 minutes, dip them in sterile absorbent paper, then soak them in 4 percent sodium hypochlorite solution for 5 minutes, rinse them with sterile water for 4 times, and cut them. Remove the browned parts at both ends, cut the middle part into tissue pieces of about 0.5 cm, inoculate them in potato dextrose solid medium containing 0.15 g / L streptomycin sulfate and 0.12 g / L penicillin potassium, and culture them upside down at 28 °C. Observe once every 12 hours. After mycelium grow...

Embodiment 2

[0037] ITS sequence molecular identification of embodiment 2 bacterial strain WZ07

[0038] Inoculate the purified WZ07 strain into a potato dextrose liquid medium containing 0.15g / L streptomycin sulfate and 0.12g / L penicillin potassium for 5 days. After the fermentation broth is centrifuged, the hyphae are ground and crushed, and the reagents are quickly extracted according to the fungal genomic DNA. DNA was extracted using the method described in the box. The sequences were amplified using universal primers ITS1 (5'-TCCGTAGGTGAACCTGCGG-3') (shown in SEQ ID No.2) and ITS4 (5'-TCCTCCGCTTATTGATATGC-3') (shown in SEQ ID No.3). The PCR reaction system is 50 μL, including: 1 μL each of ITS1 and ITS4 primers, 1 μL template DNA, 25 μL 2×PCRmix, ddH 2O 22 μL. The reaction program was: denaturation at 95°C for 3 min; denaturation at 95°C for 30 s, annealing at 58°C for 1 min, extension at 72°C for 1 min, and 30 cycles of such treatment; finally, extension at 72°C for 10 min. The PC...

Embodiment 3

[0041] The influence of embodiment 3 different temperatures on the growth of bacterial strain WZ07

[0042] Five WZ07 bacterial blocks with a diameter of 6mm were inoculated into potato dextrose liquid medium (100mL / 250mL) containing 0.15g / L streptomycin sulfate and 0.12g / L penicillin potassium, and then placed in 20, 24, 28, 32 and 36 ℃ constant temperature incubator culture in the dark for 5 days, measure the dry weight of mycelia, such as figure 2 shown. The results showed that strain WZ07 could grow at 20°C to 36°C, and the maximum growth was at 28°C, with a dry weight of 5984.33±85.51mg / L.

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Abstract

The invention discloses the endophytic fungus Letendraea sp. WZ07 and its application in nano-silver synthesis. The fungal strain was isolated from the invasive plant Trichosanthes marinum, and was deposited in the China Center for Type Culture Collection on August 4, 2020, with the preservation number CCTCC NO: M2020395. The strain was identified by the ITS sequence, which had the highest similarity (100%) to Letendraea sp.1 NV-2015, and was identified as Letendraea sp.WZ07, and the GenBank accession number of the ITS gene sequence of the strain was MN712504. The fungal strain can grow in the potato dextrose medium containing streptomycin sulfate and penicillin potassium, and its optimum temperature is 28°C and optimum pH is 5. Strain Letendraea sp.WZ07 has the ability to synthesize nanosilver and catalyze AgNO 3 The solution synthesizes nano-silver with different particle sizes and shapes. This strain expands the application of fungi in the green synthesis of nanomaterials, and also provides a new microbial resource for the biological preparation of silver nanomaterials.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, in particular to nanometer materials synthesized by microorganisms. Background technique [0002] Silver nanoparticles (AgNPs) are important noble metal nanoparticles, which have high application value in biosensor materials, low-temperature superconducting materials, catalyst materials, and biomedicine. Therefore, the synthesis and preparation of nano-silver has become a hot spot in recent analysis. There are various synthesis methods of nano-silver, mainly including physical, chemical and biological methods. Physical synthesis requires high equipment, expensive production, and high energy consumption; one of the biggest advantages of chemical synthesis is that it can synthesize a large number of nanoparticles in a short period of time, but usually relies on toxic and expensive chemical reagents, It will produce unenvironmental by-products, which limits its application in food testing, ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14C12P3/00C12R1/645
CPCC12N1/14C12P3/00C12R2001/645C12N1/145
Inventor 乔自鹏代光明王奇志
Owner HUAQIAO UNIVERSITY
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