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Pseudochrobactrum strain capable of degrading penicillin V potassium, mutant, cell fraction and composition

A technology of Bacillus pallidum and penicillin V, applied in the field of microorganisms, can solve problems such as high cost, air pollution, environmental resistance to antibiotics, etc., and achieve the effects of low treatment cost, resource saving, and elimination of adverse effects

Active Publication Date: 2020-10-27
HEBEI UNIVERSITY OF SCIENCE AND TECHNOLOGY
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Landfill disposal of fungal residues, the residual antibiotics will cause environmental drug resistance, the incineration method is not only costly, but also the carbon oxides, nitrogen oxides, and sulfur oxides produced by the combustion will cause air pollution, such as smog and acid rain question

Method used

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  • Pseudochrobactrum strain capable of degrading penicillin V potassium, mutant, cell fraction and composition
  • Pseudochrobactrum strain capable of degrading penicillin V potassium, mutant, cell fraction and composition

Examples

Experimental program
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Effect test

Embodiment 1

[0029] Example 1 Screening of Pseudomonas pallidum strains.

[0030] Step 1: Take 10 g of sludge collected from the surrounding areas of Shijiazhuang City, Hebei Province, inoculate it into 100 mL of inorganic salt medium containing 20 mg of penicillin V potassium, and culture it on a shaker at 30° C. and 160 rpm. Transfer once every 3 days after the start of culture, and transfer to a medium containing penicillin V potassium at a higher concentration with a 10% (V / V) inoculum during transfer, and the drug concentration of penicillin V potassium is 30 mg in turn / L, 60mg / L, 90mg / L, 120mg / L, 150mg / L and so on, to 900mg / L, to obtain different concentrations of enriched culture solution containing bacteria.

[0031] Wherein, the composition and the proportioning ratio of the inorganic salt culture medium in the present embodiment are (g / L): K 2 HPO 4 1.6g, KH 2 PO 4 0.4g, MgSO 4 ·7H 2 O 0.2g, CaCl 2 2H 2 O 0.025g, FeCl 3 ·6H 2 O 0.0023, NH 4 NO 3 0.5g, 20.0g agar, a...

Embodiment 2

[0039] Example 2 The screening of Pseudochrobactrum sp. in this example and its degradation effect on penicillin V potassium.

[0040] Step 1: Take 10g of sludge and put it in a sterilized 250mL Erlenmeyer flask, add 100mL of penicillin V potassium solution with a concentration of 0.3g / L, and culture on a shaker at 30°C with a rotation speed of 160r / min. After the culture starts, transfer once every 3 days, inoculate into fresh penicillin V potassium solution acclimation medium with 10% (V / V) inoculum amount during transfer, and gradually increase the content of penicillin V potassium. Detect the degradation rate of penicillin V potassium, and select the best group for the isolation of strains.

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Abstract

The invention discloses a Pseudochrobactrum strain capable of degrading penicillin V potassium. The Pseudochrobactrum strain is preserved in the China General Microbiological Culture Collection Center(CGMCC) with the preservation number of CGMCC No. 15259. The Pseudochrobactrum strain capable of degrading the penicillin V potassium can grow by utilizing the penicillin V potassium as a unique carbon source, and the degradation capacity of the strain to the penicillin V potassium with the content of 0.6g / L can reach 100% within 3 hours. The strain can be used for harmless treatment of fermentation bacterial residues in industrial production of the penicillin V potassium, the treatment method is efficient, green and environment-friendly, resources are saved, the treatment cost is low, and the adverse effect of waste hyphae of the penicillin V potassium on the environment is eliminated.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to a pseudopallidum strain capable of degrading penicillin V potassium, as well as a culture method, mutant, cell fraction and composition of the strain. Background technique [0002] Potassium penicillin V belongs to β-lactam antibiotics. Its antibacterial spectrum is similar to that of penicillin G. It can destroy bacterial cell walls and has bactericidal effect. It is also an important raw material drug and pharmaceutical precursor. Production of penicillins and cephalosporins. [0003] At present, the industrial production of penicillin V potassium is mainly made of bean cake powder, corn steep liquor, starch, etc., through fermentation, extraction, and purification. During the production process, a large amount of bacterial residue will be produced, which contains the remaining medium, metabolites of the fermentation process of antibiotic-producing bacteria, and degrada...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/34C12R1/01C02F101/38
CPCC02F3/34C02F2101/40C12R2001/01C12N1/205
Inventor 刘守信徐凯莉王鹏黄净甄小丽
Owner HEBEI UNIVERSITY OF SCIENCE AND TECHNOLOGY
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