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Frozen semen diluent formula for flocks and herds as well as making method

A technology for freezing semen and diluents, which is used in applications, preservation of human or animal bodies, animal husbandry, etc. It can solve problems such as low sperm motility, reduce sperm deformity rate, have a wide range of applications, and improve sperm motility and acrosome. The effect of completeness

Inactive Publication Date: 2012-10-17
尹旭升
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although domestic frozen semen production and artificial insemination techniques have been used for many years, the sperm motility (35%~40%) after thawing frozen semen is still lower than that of foreign countries (50%~60%)

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] (1) Preparation of base liquid: Take 1.353 g of citric acid, 2.422 g of tris (tris), and 1.1 g of glucose, and add 68.5 ml of distilled water to prepare base liquid A.

[0023] (2) Preparation of yolk liquid: select fresh eggs, sterilize the eggshells with 75% alcohol, remove the eggshells and egg whites under aseptic conditions; blot the egg whites on the surface of the yolk membrane with sterilized filter paper, and use a sterile syringe Extract the yolk.

[0024] (3) After filtering the base solution A, pasteurize it (65°C for 30 minutes). After the temperature is lower than 30°C, add 5.0ml of sterilized glycerin, 20ml of egg yolk, 6ml of bovine serum albumin (BSA) and 0.5ml of vitamin B12, and then stir evenly with a magnetic stirrer, adjust the pH value to between 6.5 and 7.5, that is, to prepare B liquid.

[0025] (4) Before use, add 50,000 IU of penicillin potassium and streptomycin sulfate to solution B respectively, and then it can be used.

[0026] (5) This ...

Embodiment 2

[0028] (1) Preparation of base liquid: Take 1.45 g of citric acid, 2.56 g of tris (tris), and 1.0 g of glucose, and add 68.4 ml of distilled water to prepare base liquid A.

[0029] (2) Preparation of yolk liquid: select fresh eggs, sterilize the eggshells with 75% alcohol, remove the eggshells and egg whites under aseptic conditions; blot the egg whites on the surface of the yolk membrane with sterilized filter paper, and use a sterile syringe Extract the yolk.

[0030] (3) After filtering the base solution A, pasteurize it (65°C for 30 minutes). After the temperature is lower than 30°C, add 6.5ml of sterilized glycerin, 18ml of egg yolk, and 6.5ml of bovine serum albumin (BSA) and 0.6ml of vitamin B12, then stir evenly with a magnetic stirrer, and adjust the pH value to between 6.5 and 7.5, that is, to prepare B liquid.

[0031] (4) Before use, add 75,000 IU of penicillin potassium and streptomycin sulfate to solution B respectively, and then it can be used.

Embodiment 3

[0033] (1) Preparation of base liquid: Take 1.55 g of citric acid, 2.48 g of tris (tris), and 1.2 g of glucose, and add 64.8 ml of distilled water to prepare base liquid A.

[0034] (2) Preparation of yolk liquid: select fresh eggs, sterilize the eggshells with 75% alcohol, remove the eggshells and egg whites under aseptic conditions; blot the egg whites on the surface of the yolk membrane with sterilized filter paper, and use a sterile syringe Extract the yolk.

[0035] (3) After filtering the base solution A, pasteurize it (65°C for 30 minutes). After the temperature is lower than 30°C, add 6.0ml of sterilized glycerin, 21ml of egg yolk, and 7.5ml of bovine serum albumin (BSA) and 0.7ml of vitamin B12, and then stir evenly with a magnetic stirrer, adjust the pH value to between 6.5 and 7.5, and then prepare B liquid.

[0036] (4) Before use, add 100,000 IU of penicillin potassium and streptomycin sulfate to solution B respectively, and then it can be used.

[0037] It shou...

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Abstract

The invention provides a frozen semen diluent formula for flocks and herds, and the formula of each 100mL of diluent comprises the following components: 1.35-1.55g of citric acid, 2.4-2.6g of trihydroxymethyl aminomethane (tris), 1.0-1.2g of glucose, 4.5-6.8ml of glycerol, 18.0-21ml of yolk, 4.5-7.5ml of bovine serum albumin (BSA), 0.45-0.75ml of vitamin B12, 50000-100000IU of benzylpenicillin potassium, 50000-100000IU of streptomycin sulfate and the balance of distilled water. The formula provided by the invention is low in cost, simple and convenient in preparation and using and operation steps, capable of effectively improving the sperm motility, the acrosome integrity rate and the plasmalemma integrity rate, and capable of reducing the sperm aberration rate.

Description

technical field [0001] The invention relates to a formula and a preparation method of a frozen semen diluent for cattle and sheep. Background technique [0002] The diluent is an essential component in the freezing of bovine, sheep and other semen, which can dilute, buffer, nourish and protect the sperm during the freezing process, and effectively reduce the impact of low temperature on the sperm. Although domestic frozen semen production and artificial insemination techniques have been used for many years, the sperm motility (35%-40%) after thawing frozen semen is still lower than that of foreign countries (50%-60%). After the semen is frozen and thawed at ultra-low temperature, the motility of the sperm is reduced, and the number of deaths and the deformity rate are increased. and fertilization capacity. Contents of the invention [0003] The object of the present invention is to provide a frozen semen diluent formula and preparation method for cattle and sheep that ca...

Claims

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Application Information

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IPC IPC(8): A01N1/02
Inventor 尹旭升牛华星张淑二田宁宁霍道坦
Owner 尹旭升
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